prrsv antibody enzyme linked immunosorbent assay elisa kit  (IDEXX)

Journal: The Journal of Veterinary Medical Science

Article Title: Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors

doi: 10.1292/jvms.15-0126

Figure Lengend Snippet: Test results from two commercial PRRS ELISA kits applied to 476 serum samples sequentially collected from PRRS-negative pigs challenged with 7 different PRRSV strains. *The horizontal line indicates the cut-off value (0.4) for positivity.

Article Snippet: In this study, the sensitivity and specificity of two commercial PRRSV antibody (Ab) ELISA kits, IDEXX PRRS 3XR Ab ELISA (Westbrook, ME, U.S.A.) and Bionote PRRS Ab ELISA 4.0 (Hwasung, South Korea), are compared using samples collected from animal challenge studies with various PRRSV strains as well as field samples from Korean PRRS-negative swine farms.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Sustaining Interferon Induction by a High-Passage Atypical Porcine Reproductive and Respiratory Syndrome Virus Strain

doi: 10.1038/srep36312

Figure Lengend Snippet: Serological testing of serum samples from pig studies. Four pigs from each group were infected. Mock-infected pigs (PBS) were included as controls. ( A ) ELISA of PRRSV antibodies in serum samples of 35 days post-infection (DPI). The S/P ratio above 0.4 is considered positive. Error bars represent standard errors of the ratios among the four pigs in each group. A2: A2MC2. ( B ) Virus-neutralization antibody assay against PRRSV VR-2332. The virus neutralization (VN) titers are shown as reciprocals of serum dilutions shown VN activity. Significant differences between the A2MC2 groups and the group of MLV-infected pigs are denoted by *and **, which indicate P

Article Snippet: To assess the antibody response against PRRSV in the pigs, serum samples of DPI35 was tested with a commercial PRRSV ELISA kit (IDEXX PRRS X3 Ab Test; IDEXX Inc., Westbrook, MA, USA) according to the manufacturer’s instructions.

Techniques: Infection, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay

Journal: Journal of Veterinary Science

Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

doi: 10.4142/jvs.2018.19.3.358

Figure Lengend Snippet: Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

Article Snippet: PRRSV-specific antibodies were detected by using a commercial PRRSV antibody enzyme-linked immunosorbent assay (ELISA) kit (HerdChek PRRS X3; IDEXX Laboratories, USA), according to the manufacturer's protocols, and a serum neutralization test as described previously [ ].

Techniques: In Vivo, Infection, Enzyme-linked Immunosorbent Assay

Journal: The Open Virology Journal

Article Title: Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

doi: 10.2174/1874357901711010073

Figure Lengend Snippet: Serum immune cytokine profiles after PRRSV infection. Cytokine expression profiles in the sera (A) of challenge pigs 6 days post infection (DPI) and supernatants of lung homogenates (B) were tested by quantitative ELISA. Data were shown as mean ± SEM for 5 pigs per group. One asterisk denotes a statistically significant difference (P

Article Snippet: Serum was used for evaluation of viral titer and PRRSV-specific ELISA antibody titers (Herdchek Porcine Reproductive and Respiratory Syndrome Antibody test Kit, IDEXX Laboratories) as previously described [ ].

Techniques: Infection, Expressing, Enzyme-linked Immunosorbent Assay

Journal: The Open Virology Journal

Article Title: Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

doi: 10.2174/1874357901711010073

Figure Lengend Snippet: Viremia and PRRSV-specific ELISA Ab profiles after PRRSV infection. (A) PRRSV viral RNA in the serum was determined by real time RT-PCR. (B) Pig serum was assayed for PRRSV-specific antibodies with IDEXX HerdCheck ELISA. The threshold for seroconversion was set at a sample-to-positive (s/p) ratio of 0.4 according to manufacturers’ instructions. Each bar represents the average of five pigs ± SEM. *p

Article Snippet: Serum was used for evaluation of viral titer and PRRSV-specific ELISA antibody titers (Herdchek Porcine Reproductive and Respiratory Syndrome Antibody test Kit, IDEXX Laboratories) as previously described [ ].

Techniques: Enzyme-linked Immunosorbent Assay, Infection, Quantitative RT-PCR