prrsv antibody enzyme linked immunosorbent assay elisa kit  (IDEXX)

 
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    Structured Review

    IDEXX prrsv antibody enzyme linked immunosorbent assay elisa kit
    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked <t>immunosorbent</t> assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p
    Prrsv Antibody Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prrsv antibody enzyme linked immunosorbent assay elisa kit/product/IDEXX
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prrsv antibody enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages"

    Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

    Journal: Journal of Veterinary Science

    doi: 10.4142/jvs.2018.19.3.358

    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p
    Figure Legend Snippet: Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

    Techniques Used: In Vivo, Infection, Enzyme-linked Immunosorbent Assay

    2) Product Images from "Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages"

    Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

    Journal: Journal of Veterinary Science

    doi: 10.4142/jvs.2018.19.3.358

    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p
    Figure Legend Snippet: Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

    Techniques Used: In Vivo, Infection, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors
    Article Snippet: .. In this study, the sensitivity and specificity of two commercial PRRSV antibody (Ab) ELISA kits, IDEXX PRRS 3XR Ab ELISA (Westbrook, ME, U.S.A.) and Bionote PRRS Ab ELISA 4.0 (Hwasung, South Korea), are compared using samples collected from animal challenge studies with various PRRSV strains as well as field samples from Korean PRRS-negative swine farms. .. Four hundred seventy-six serum samples were selected from 7 different animal challenge studies that were conducted with 7 different North America (type II) PRRSV strains [VR2332 (GenBank accession number: JF430265), JA142 (AY424271), SDSU73 (JN654458), MN184 (EF488739), K07-2273 (JQ656251), K08-1053 (JQ656266) or Ingelvac PRRS MLV (hereafter “MLV”, AF159149)] that share 88–99% nucleotide sequence homology based on ORF5 sequences.

    Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages
    Article Snippet: Viremia and serology PRRSV levels in serum samples were assessed by virus titration using MARC-145 or PAM-pCD163 cells as described previously [ ]. .. PRRSV-specific antibodies were detected by using a commercial PRRSV antibody enzyme-linked immunosorbent assay (ELISA) kit (HerdChek PRRS X3; IDEXX Laboratories, USA), according to the manufacturer's protocols, and a serum neutralization test as described previously [ ]. .. Nucleotide sequence analysis The full-length genomic sequences of CA-2-P100 strains at the 10th and 20th passages in PAM-KNU cells, designated CA-2-MP110 and CA-2-MP120, were determined by next-generation sequencing (NGS) technology as previously described [ ].

    Article Title: Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain).
    Article Snippet: .. The classical swine fever (CSF) vaccine, which is derived from the LOM strain of the CSF virus (CSFV), induces protective immunity against CSFV infection. .. The classical swine fever (CSF) vaccine, which is derived from the LOM strain of the CSF virus (CSFV), induces protective immunity against CSFV infection.

    Article Title: Ginsenoside Rg1 Suppresses Type 2 PRRSV Infection via NF-κB Signaling Pathway In Vitro, and Provides Partial Protection against HP-PRRSV in Piglet
    Article Snippet: The group Rg1 was injected with Rg1 (10 mg/kg/day) and continued administration for 10 days, being used as the drug control group. .. Blood samples of each piglet were obtained by venipuncture at 0, 2, 4, 7, 10 and 14 days post-infection (dpi) for the detection of the PRRSV viral load and anti-PRRSV antibody (Idexx PRRSV Elisa Kit, Idexx, US). ..

    Article Title: Immune Response of Multiparous Hyper-Immunized Sows against Peptides from Non-Structural and Structural Proteins of PRRSV
    Article Snippet: For the IFN-γ ELISPOT assay, the peptides were used at a final concentration of 10 µg/mL in the single peptide solution and in the pooled peptides solution. .. IDEXX ELISA Antibodies against PRRSV were detected using a commercial ELISA kit (Herdchek Porcine Reproductive and Respiratory Syndrome Antibody Test Kit, IDEXX Laboratories, Westbrook, Maine) according to the manufacturer’s directions. .. Peptide ELISA Sera samples were used to detect peptide-specific antibodies as reported by de Lima et al. [ ].

    Neutralization:

    Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages
    Article Snippet: Viremia and serology PRRSV levels in serum samples were assessed by virus titration using MARC-145 or PAM-pCD163 cells as described previously [ ]. .. PRRSV-specific antibodies were detected by using a commercial PRRSV antibody enzyme-linked immunosorbent assay (ELISA) kit (HerdChek PRRS X3; IDEXX Laboratories, USA), according to the manufacturer's protocols, and a serum neutralization test as described previously [ ]. .. Nucleotide sequence analysis The full-length genomic sequences of CA-2-P100 strains at the 10th and 20th passages in PAM-KNU cells, designated CA-2-MP110 and CA-2-MP120, were determined by next-generation sequencing (NGS) technology as previously described [ ].

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    IDEXX prrsv antibody ab elisa kits
    Test results from two commercial PRRS <t>ELISA</t> kits applied to 476 serum samples sequentially collected from PRRS-negative pigs challenged with 7 different <t>PRRSV</t> strains. *The horizontal line indicates the cut-off value (0.4) for positivity.
    Prrsv Antibody Ab Elisa Kits, supplied by IDEXX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prrsv antibody ab elisa kits/product/IDEXX
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prrsv antibody ab elisa kits - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    99
    IDEXX prrsv elisa kit
    Serological testing of serum samples from pig studies. Four pigs from each group were infected. Mock-infected pigs (PBS) were included as controls. ( A ) <t>ELISA</t> of <t>PRRSV</t> antibodies in serum samples of 35 days post-infection (DPI). The S/P ratio above 0.4 is considered positive. Error bars represent standard errors of the ratios among the four pigs in each group. A2: A2MC2. ( B ) Virus-neutralization antibody assay against PRRSV VR-2332. The virus neutralization (VN) titers are shown as reciprocals of serum dilutions shown VN activity. Significant differences between the A2MC2 groups and the group of MLV-infected pigs are denoted by *and **, which indicate P
    Prrsv Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prrsv elisa kit/product/IDEXX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prrsv elisa kit - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    86
    IDEXX prrsv antibody enzyme linked immunosorbent assay elisa kit
    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked <t>immunosorbent</t> assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p
    Prrsv Antibody Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prrsv antibody enzyme linked immunosorbent assay elisa kit/product/IDEXX
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prrsv antibody enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    99
    IDEXX prrsv specific elisa antibody titers
    Serum immune cytokine profiles after <t>PRRSV</t> infection. Cytokine expression profiles in the sera (A) of challenge pigs 6 days post infection (DPI) and supernatants of lung homogenates (B) were tested by quantitative <t>ELISA.</t> Data were shown as mean ± SEM for 5 pigs per group. One asterisk denotes a statistically significant difference (P
    Prrsv Specific Elisa Antibody Titers, supplied by IDEXX, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prrsv specific elisa antibody titers/product/IDEXX
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prrsv specific elisa antibody titers - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Test results from two commercial PRRS ELISA kits applied to 476 serum samples sequentially collected from PRRS-negative pigs challenged with 7 different PRRSV strains. *The horizontal line indicates the cut-off value (0.4) for positivity.

    Journal: The Journal of Veterinary Medical Science

    Article Title: Evaluation of two commercial PRRSV antibody ELISA kits with samples of known status and singleton reactors

    doi: 10.1292/jvms.15-0126

    Figure Lengend Snippet: Test results from two commercial PRRS ELISA kits applied to 476 serum samples sequentially collected from PRRS-negative pigs challenged with 7 different PRRSV strains. *The horizontal line indicates the cut-off value (0.4) for positivity.

    Article Snippet: In this study, the sensitivity and specificity of two commercial PRRSV antibody (Ab) ELISA kits, IDEXX PRRS 3XR Ab ELISA (Westbrook, ME, U.S.A.) and Bionote PRRS Ab ELISA 4.0 (Hwasung, South Korea), are compared using samples collected from animal challenge studies with various PRRSV strains as well as field samples from Korean PRRS-negative swine farms.

    Techniques: Enzyme-linked Immunosorbent Assay

    Serological testing of serum samples from pig studies. Four pigs from each group were infected. Mock-infected pigs (PBS) were included as controls. ( A ) ELISA of PRRSV antibodies in serum samples of 35 days post-infection (DPI). The S/P ratio above 0.4 is considered positive. Error bars represent standard errors of the ratios among the four pigs in each group. A2: A2MC2. ( B ) Virus-neutralization antibody assay against PRRSV VR-2332. The virus neutralization (VN) titers are shown as reciprocals of serum dilutions shown VN activity. Significant differences between the A2MC2 groups and the group of MLV-infected pigs are denoted by *and **, which indicate P

    Journal: Scientific Reports

    Article Title: Sustaining Interferon Induction by a High-Passage Atypical Porcine Reproductive and Respiratory Syndrome Virus Strain

    doi: 10.1038/srep36312

    Figure Lengend Snippet: Serological testing of serum samples from pig studies. Four pigs from each group were infected. Mock-infected pigs (PBS) were included as controls. ( A ) ELISA of PRRSV antibodies in serum samples of 35 days post-infection (DPI). The S/P ratio above 0.4 is considered positive. Error bars represent standard errors of the ratios among the four pigs in each group. A2: A2MC2. ( B ) Virus-neutralization antibody assay against PRRSV VR-2332. The virus neutralization (VN) titers are shown as reciprocals of serum dilutions shown VN activity. Significant differences between the A2MC2 groups and the group of MLV-infected pigs are denoted by *and **, which indicate P

    Article Snippet: To assess the antibody response against PRRSV in the pigs, serum samples of DPI35 was tested with a commercial PRRSV ELISA kit (IDEXX PRRS X3 Ab Test; IDEXX Inc., Westbrook, MA, USA) according to the manufacturer’s instructions.

    Techniques: Infection, Enzyme-linked Immunosorbent Assay, Neutralization, Activity Assay

    Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

    Journal: Journal of Veterinary Science

    Article Title: Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

    doi: 10.4142/jvs.2018.19.3.358

    Figure Lengend Snippet: Virus replication in vivo and seroconversion. (A) Mean levels of viremia of pigs infected with CA-2-P20, CA-2-P100, or CA-2-MP120. TCID 50 , 50% tissue culture infectious dose. (B) Virus-specific antibody response of pigs measured by using a commercial enzyme-linked immunosorbent assay kit. Serum samples were considered positive for antibodies to porcine reproductive and respiratory syndrome virus if the sample:positive (S/P) ratio was equal to or greater than 0.4. Error bars represent SD. “a” denotes a significant difference ( p

    Article Snippet: PRRSV-specific antibodies were detected by using a commercial PRRSV antibody enzyme-linked immunosorbent assay (ELISA) kit (HerdChek PRRS X3; IDEXX Laboratories, USA), according to the manufacturer's protocols, and a serum neutralization test as described previously [ ].

    Techniques: In Vivo, Infection, Enzyme-linked Immunosorbent Assay

    Serum immune cytokine profiles after PRRSV infection. Cytokine expression profiles in the sera (A) of challenge pigs 6 days post infection (DPI) and supernatants of lung homogenates (B) were tested by quantitative ELISA. Data were shown as mean ± SEM for 5 pigs per group. One asterisk denotes a statistically significant difference (P

    Journal: The Open Virology Journal

    Article Title: Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

    doi: 10.2174/1874357901711010073

    Figure Lengend Snippet: Serum immune cytokine profiles after PRRSV infection. Cytokine expression profiles in the sera (A) of challenge pigs 6 days post infection (DPI) and supernatants of lung homogenates (B) were tested by quantitative ELISA. Data were shown as mean ± SEM for 5 pigs per group. One asterisk denotes a statistically significant difference (P

    Article Snippet: Serum was used for evaluation of viral titer and PRRSV-specific ELISA antibody titers (Herdchek Porcine Reproductive and Respiratory Syndrome Antibody test Kit, IDEXX Laboratories) as previously described [ ].

    Techniques: Infection, Expressing, Enzyme-linked Immunosorbent Assay

    Viremia and PRRSV-specific ELISA Ab profiles after PRRSV infection. (A) PRRSV viral RNA in the serum was determined by real time RT-PCR. (B) Pig serum was assayed for PRRSV-specific antibodies with IDEXX HerdCheck ELISA. The threshold for seroconversion was set at a sample-to-positive (s/p) ratio of 0.4 according to manufacturers’ instructions. Each bar represents the average of five pigs ± SEM. *p

    Journal: The Open Virology Journal

    Article Title: Comparison of Immune Responses in Pigs Infected with Chinese Highly Pathogenic PRRS Virus Strain HV and North American Strain NADC-20

    doi: 10.2174/1874357901711010073

    Figure Lengend Snippet: Viremia and PRRSV-specific ELISA Ab profiles after PRRSV infection. (A) PRRSV viral RNA in the serum was determined by real time RT-PCR. (B) Pig serum was assayed for PRRSV-specific antibodies with IDEXX HerdCheck ELISA. The threshold for seroconversion was set at a sample-to-positive (s/p) ratio of 0.4 according to manufacturers’ instructions. Each bar represents the average of five pigs ± SEM. *p

    Article Snippet: Serum was used for evaluation of viral titer and PRRSV-specific ELISA antibody titers (Herdchek Porcine Reproductive and Respiratory Syndrome Antibody test Kit, IDEXX Laboratories) as previously described [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, Quantitative RT-PCR