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Bio-Rad proteins concentration
Proteins Concentration, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteins concentration - by Bioz Stars, 2020-04
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Autoradiography:

Article Title: Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2
Article Snippet: Bio-Rad protein assay was used to determine proteins concentration (BioRAD, Hercules, CA, USA). .. Proteins were detected by autoradiography and bands densitometry was estimated using a Discover Pharmacia scanner equipped with a sun spark classic densitometric workstation .

Concentration Assay:

Article Title: Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2
Article Snippet: .. Bio-Rad protein assay was used to determine proteins concentration (BioRAD, Hercules, CA, USA). .. Fifty micrograms of whole lysates were resolved on 10% SDS-PAGE and proteins were transferred by blotting onto PVDF membranes.

Incubation:

Article Title: Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2
Article Snippet: Briefly, cells were washed in cold phosphate buffered saline (PBS) and lysed by incubation with RIPA buffer (50 mM Tris–HCl, pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 0.25% sodium deoxycholate, 1 mM NaF, 10 μM Na3 VO4 , 1 mM phenylmethylsulfonylfluoride, 10 μg/ml aprotinin, 10 μg/ml pepstatin, 10 μg/ml leupeptin) for 45 min at 4 °C . .. Bio-Rad protein assay was used to determine proteins concentration (BioRAD, Hercules, CA, USA).

Western Blot:

Article Title: Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2
Article Snippet: Paragraph title: Western blotting ... Bio-Rad protein assay was used to determine proteins concentration (BioRAD, Hercules, CA, USA).

SDS Page:

Article Title: Phosphoproteomic analysis sheds light on intracellular signaling cascades triggered by Formyl-Peptide Receptor 2
Article Snippet: Bio-Rad protein assay was used to determine proteins concentration (BioRAD, Hercules, CA, USA). .. Fifty micrograms of whole lysates were resolved on 10% SDS-PAGE and proteins were transferred by blotting onto PVDF membranes.

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  • 92
    Bio-Rad γ secretase
    Phenylalanine blocking mutations at both ε cleavage sites reduces APP cleavage but not binding to <t>γ-secretase.</t> ( A ) Western blot of γ-secretase cleavage of WT, V50F, M51F and V50F-M51F C100-FLAG. Duplicates from each substrate represent separate independent data points. * denotes a degradation product which co-purified with the substrate. ( B ) Cleavage of WT and V50F-M51F C100-FLAG over time. ( C ) Co-immunoprecipitation of Myc-tagged WT or V50F-M51F C100 substrate. Duplicates are from separate pull-down experiments. * antibody light chain. ( D ) Competitive cleavage of WT C100-FLAG by WT C100-Myc or V50F-M51F C100-Myc. ( E ) Aβ42/40 ratio of the V50F-M51F double mutant. Mean ± SD, n = 3, t-test, ****
    γ Secretase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ secretase/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    γ secretase - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    93
    Bio-Rad chemokine concentrations
    Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and <t>chemokine</t> concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.
    Chemokine Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/chemokine concentrations/product/Bio-Rad
    Average 93 stars, based on 4 article reviews
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    chemokine concentrations - by Bioz Stars, 2020-04
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    92
    Bio-Rad ikbke total cell protein concentrations
    A. <t>IKBKe</t> transcription in <t>MDA-MB-231</t> cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p
    Ikbke Total Cell Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ikbke total cell protein concentrations - by Bioz Stars, 2020-04
    92/100 stars
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    Image Search Results


    Phenylalanine blocking mutations at both ε cleavage sites reduces APP cleavage but not binding to γ-secretase. ( A ) Western blot of γ-secretase cleavage of WT, V50F, M51F and V50F-M51F C100-FLAG. Duplicates from each substrate represent separate independent data points. * denotes a degradation product which co-purified with the substrate. ( B ) Cleavage of WT and V50F-M51F C100-FLAG over time. ( C ) Co-immunoprecipitation of Myc-tagged WT or V50F-M51F C100 substrate. Duplicates are from separate pull-down experiments. * antibody light chain. ( D ) Competitive cleavage of WT C100-FLAG by WT C100-Myc or V50F-M51F C100-Myc. ( E ) Aβ42/40 ratio of the V50F-M51F double mutant. Mean ± SD, n = 3, t-test, ****

    Journal: eLife

    Article Title: The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase

    doi: 10.7554/eLife.17578

    Figure Lengend Snippet: Phenylalanine blocking mutations at both ε cleavage sites reduces APP cleavage but not binding to γ-secretase. ( A ) Western blot of γ-secretase cleavage of WT, V50F, M51F and V50F-M51F C100-FLAG. Duplicates from each substrate represent separate independent data points. * denotes a degradation product which co-purified with the substrate. ( B ) Cleavage of WT and V50F-M51F C100-FLAG over time. ( C ) Co-immunoprecipitation of Myc-tagged WT or V50F-M51F C100 substrate. Duplicates are from separate pull-down experiments. * antibody light chain. ( D ) Competitive cleavage of WT C100-FLAG by WT C100-Myc or V50F-M51F C100-Myc. ( E ) Aβ42/40 ratio of the V50F-M51F double mutant. Mean ± SD, n = 3, t-test, ****

    Article Snippet: In vitro γ-secretase assay Purified γ-secretase was incorporated into vesicles by first dissolving total brain lipid extract (1.25 mM final) in 50 mM HEPES pH 7.0, 150 mM NaCl, 0.25% CHAPSO. γ-Secretase (5–30 nM final concentration) was then added to the solution and detergent removed by mixing SM-2 biobeads (62 mg/mL) (Bio-Rad) with the lipid/detergent/enzyme solution for two hrs at 4°C.

    Techniques: Blocking Assay, Binding Assay, Western Blot, Purification, Immunoprecipitation, Mutagenesis

    Tripeptide fragments of APP inhibit γ-secretase. ( A ) Schematic diagram of the major sequential cleavage pathways of the transmembrane domain of APP (Aβ49 → Aβ46 → Aβ43 → Aβ40 in red and Aβ48 → Aβ45 → Aβ42 in blue). Mutations causing Familial Alzheimer’s disease are below the APP TMD in blue. ( B ) IC50 curves from the inhibition of γ-secretase activity by APP product tripeptide fragments. Mean ± SD, n = 2. ( C ) Noncompetitive inhibition of γ-secretase with VIV tripeptide, R 2 = 0.98. ( D ) Yonetani-Theorell plot for the mutually exclusive binding of VIV and the noncompetitive transition-state analog inhibitor III-31-C, R 2 = 0.98. ( E ) Cartoon representation of the three S’ pockets of presenilin (PSEN) along with three P’ amino acids of substrate and the transition-state analog L685,458. ( F ) IC50 curves from the inhibition of γ-secretase activity with FAF and AFA synthetic tripeptides. Mean ± SD, n = 2. DOI: http://dx.doi.org/10.7554/eLife.17578.003

    Journal: eLife

    Article Title: The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase

    doi: 10.7554/eLife.17578

    Figure Lengend Snippet: Tripeptide fragments of APP inhibit γ-secretase. ( A ) Schematic diagram of the major sequential cleavage pathways of the transmembrane domain of APP (Aβ49 → Aβ46 → Aβ43 → Aβ40 in red and Aβ48 → Aβ45 → Aβ42 in blue). Mutations causing Familial Alzheimer’s disease are below the APP TMD in blue. ( B ) IC50 curves from the inhibition of γ-secretase activity by APP product tripeptide fragments. Mean ± SD, n = 2. ( C ) Noncompetitive inhibition of γ-secretase with VIV tripeptide, R 2 = 0.98. ( D ) Yonetani-Theorell plot for the mutually exclusive binding of VIV and the noncompetitive transition-state analog inhibitor III-31-C, R 2 = 0.98. ( E ) Cartoon representation of the three S’ pockets of presenilin (PSEN) along with three P’ amino acids of substrate and the transition-state analog L685,458. ( F ) IC50 curves from the inhibition of γ-secretase activity with FAF and AFA synthetic tripeptides. Mean ± SD, n = 2. DOI: http://dx.doi.org/10.7554/eLife.17578.003

    Article Snippet: In vitro γ-secretase assay Purified γ-secretase was incorporated into vesicles by first dissolving total brain lipid extract (1.25 mM final) in 50 mM HEPES pH 7.0, 150 mM NaCl, 0.25% CHAPSO. γ-Secretase (5–30 nM final concentration) was then added to the solution and detergent removed by mixing SM-2 biobeads (62 mg/mL) (Bio-Rad) with the lipid/detergent/enzyme solution for two hrs at 4°C.

    Techniques: Inhibition, Activity Assay, Binding Assay

    Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Journal: PLoS ONE

    Article Title: Attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice

    doi: 10.1371/journal.pone.0196037

    Figure Lengend Snippet: Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Article Snippet: The protein concentration of the resulting supernatant was determined with a DC (detergent-compatible) protein assay (Bio-Rad, Hercules, CA), and the supernatant was then stored at –80°C until assay of cytokine and chemokine concentrations with the use of a Bio-Plex Pro Mouse Cytokine 23-Plex Panel and Bio-Plex Manager software version 4.1.1 (Bio-Rad).

    Techniques: Mouse Assay, Gas Chromatography, Immunohistofluorescence

    Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Journal: PLoS ONE

    Article Title: Attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice

    doi: 10.1371/journal.pone.0196037

    Figure Lengend Snippet: Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Article Snippet: The protein concentration of the resulting supernatant was determined with a DC (detergent-compatible) protein assay (Bio-Rad, Hercules, CA), and the supernatant was then stored at –80°C until assay of cytokine and chemokine concentrations with the use of a Bio-Plex Pro Mouse Cytokine 23-Plex Panel and Bio-Plex Manager software version 4.1.1 (Bio-Rad).

    Techniques: Mouse Assay, Gas Chromatography, Immunohistofluorescence

    A. IKBKe transcription in MDA-MB-231 cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p

    Journal: PLoS ONE

    Article Title: Apigenin inhibits TNFα/IL-1α-induced CCL2 release through IKBK-epsilon signaling in MDA-MB-231 human breast cancer cells

    doi: 10.1371/journal.pone.0175558

    Figure Lengend Snippet: A. IKBKe transcription in MDA-MB-231 cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p

    Article Snippet: Western blot ERK1/2 and IKBKe Total cell protein concentrations from MDA-MB-231 cells treated with apigenin, with and without TNFα co-treatment, was determined using a modified Bio-Rad “DC” protein assay (Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Multiple Displacement Amplification, Expressing