proteinase k  (Thermo Fisher)


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    Name:
    Proteinase K
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    f-202s
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    Structured Review

    Thermo Fisher proteinase k
    Effects of <t>proteinase</t> K and heat on the antifungal activity of culture filtrates of Trichoderma strain SDRLIN1to phytopathogenic fungi.

    https://www.bioz.com/result/proteinase k/product/Thermo Fisher
    Average 99 stars, based on 727 article reviews
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    proteinase k - by Bioz Stars, 2020-01
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    Images

    1) Product Images from "Antagonistic Potential of Native Trichoderma viride Strain against Potent Tea Fungal Pathogens in North East India"

    Article Title: Antagonistic Potential of Native Trichoderma viride Strain against Potent Tea Fungal Pathogens in North East India

    Journal: The Plant Pathology Journal

    doi: 10.5423/PPJ.OA.01.2015.0004

    Effects of proteinase K and heat on the antifungal activity of culture filtrates of Trichoderma strain SDRLIN1to phytopathogenic fungi.
    Figure Legend Snippet: Effects of proteinase K and heat on the antifungal activity of culture filtrates of Trichoderma strain SDRLIN1to phytopathogenic fungi.

    Techniques Used: Activity Assay

    2) Product Images from "Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿"

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.06042-11

    Chelation-dependent surface exposure of OspA-CaM fusion proteins at steady state. (A) Accessibility of full-length OspA-CaM or OspA tether-CaM fusion proteins to proteinase K. Cells expressing the fusion constructs were grown in BSK-SFcc containing either
    Figure Legend Snippet: Chelation-dependent surface exposure of OspA-CaM fusion proteins at steady state. (A) Accessibility of full-length OspA-CaM or OspA tether-CaM fusion proteins to proteinase K. Cells expressing the fusion constructs were grown in BSK-SFcc containing either

    Techniques Used: Chick Chorioallantoic Membrane Assay, Expressing, Construct

    3) Product Images from "Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues"

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues

    Journal: Cartilage

    doi: 10.1177/1947603517690856

    Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with
    Figure Legend Snippet: Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with

    Techniques Used: Incubation

    4) Product Images from "Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues"

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues

    Journal: Cartilage

    doi: 10.1177/1947603517690856

    Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with
    Figure Legend Snippet: Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with

    Techniques Used: Incubation

    5) Product Images from "Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins"

    Article Title: Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0071751

    The pro-inflammatory response of HEp-2 cells to A. baumannii OMVs treated with proteinase K and EDTA. (A) Protein profiles of A. baumannii OMVs. Lane M, size marker; 1, purified intact OMVs; 2, OMVs treated with EDTA; 3, OMVs treated with proteinase K; 4, proteinase K. (B) Expression of pro-inflammatory cytokine genes to proteinase K- and EDTA-treated A. baumannii OMVs was assessed by quantitative real-time PCR. HEp-2 cells were treated with the same concentration (15 µg/ml) of A. baumannii OMVs for 24 h as a positive control. Data are presented as mean ± SD of duplicate determinations.
    Figure Legend Snippet: The pro-inflammatory response of HEp-2 cells to A. baumannii OMVs treated with proteinase K and EDTA. (A) Protein profiles of A. baumannii OMVs. Lane M, size marker; 1, purified intact OMVs; 2, OMVs treated with EDTA; 3, OMVs treated with proteinase K; 4, proteinase K. (B) Expression of pro-inflammatory cytokine genes to proteinase K- and EDTA-treated A. baumannii OMVs was assessed by quantitative real-time PCR. HEp-2 cells were treated with the same concentration (15 µg/ml) of A. baumannii OMVs for 24 h as a positive control. Data are presented as mean ± SD of duplicate determinations.

    Techniques Used: Marker, Purification, Expressing, Real-time Polymerase Chain Reaction, Concentration Assay, Positive Control

    6) Product Images from "Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice"

    Article Title: Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0128510

    No change in Proteinase-K-resistant aggregates in curcumin diet mice. (A) Western blot detection of Syn-GFP microaggregates, following Proteinase-K digestion of synaptosome and cytosolic protein fractions from control and curcumin diet mice. (B) Quantification of Syn-GFP band intensity shows no change in the resistant fraction between control and curcumin diet mice.
    Figure Legend Snippet: No change in Proteinase-K-resistant aggregates in curcumin diet mice. (A) Western blot detection of Syn-GFP microaggregates, following Proteinase-K digestion of synaptosome and cytosolic protein fractions from control and curcumin diet mice. (B) Quantification of Syn-GFP band intensity shows no change in the resistant fraction between control and curcumin diet mice.

    Techniques Used: Mouse Assay, Western Blot

    7) Product Images from "Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress"

    Article Title: Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress

    Journal: Cellular and Molecular Bioengineering

    doi: 10.1007/s12195-018-0552-9

    Dissociation of bacterial aggregates by proteinase K treatment. (a) The cartoon demonstrates the experimental setup using proteinase K to disperse adhesion-based bacterial aggregates. (b) Comparison of the number of aggregates found in samples expressing eCPX-Dockerin-Ac after treatment with proteinase K to untreated samples. * indicates that differences between samples are significant below a p-value less than 0.05. Significance was determined by a one-tailed t test with an unequal variance that returned a p-value of 0.013. (c) Control sample demonstrating normal aggregate formation by expression of eCPX-Dockerin-Ac. Red circles highlight aggregates. (d) Control sample demonstrating normal planktonic state of BL21DE3 cells. (e) An experimental sample of cells expressing eCPX-Dockerin-Ac treated with 0.1 μ L/mL proteinase K demonstrating dissociation of aggregates found in the untreated sample (b) . (f) Control sample demonstrating the normal planktonic state of BL21DE3 cells treated with 0.1 μ L/mL proteinase K. (c–f) ”).
    Figure Legend Snippet: Dissociation of bacterial aggregates by proteinase K treatment. (a) The cartoon demonstrates the experimental setup using proteinase K to disperse adhesion-based bacterial aggregates. (b) Comparison of the number of aggregates found in samples expressing eCPX-Dockerin-Ac after treatment with proteinase K to untreated samples. * indicates that differences between samples are significant below a p-value less than 0.05. Significance was determined by a one-tailed t test with an unequal variance that returned a p-value of 0.013. (c) Control sample demonstrating normal aggregate formation by expression of eCPX-Dockerin-Ac. Red circles highlight aggregates. (d) Control sample demonstrating normal planktonic state of BL21DE3 cells. (e) An experimental sample of cells expressing eCPX-Dockerin-Ac treated with 0.1 μ L/mL proteinase K demonstrating dissociation of aggregates found in the untreated sample (b) . (f) Control sample demonstrating the normal planktonic state of BL21DE3 cells treated with 0.1 μ L/mL proteinase K. (c–f) ”).

    Techniques Used: Expressing, One-tailed Test

    8) Product Images from "Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress"

    Article Title: Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress

    Journal: Cellular and Molecular Bioengineering

    doi: 10.1007/s12195-018-0552-9

    Dissociation of bacterial aggregates by proteinase K treatment. (a) The cartoon demonstrates the experimental setup using proteinase K to disperse adhesion-based bacterial aggregates. (b) Comparison of the number of aggregates found in samples expressing eCPX-Dockerin-Ac after treatment with proteinase K to untreated samples. * indicates that differences between samples are significant below a p-value less than 0.05. Significance was determined by a one-tailed t test with an unequal variance that returned a p-value of 0.013. (c) Control sample demonstrating normal aggregate formation by expression of eCPX-Dockerin-Ac. Red circles highlight aggregates. (d) Control sample demonstrating normal planktonic state of BL21DE3 cells. (e) An experimental sample of cells expressing eCPX-Dockerin-Ac treated with 0.1 μ L/mL proteinase K demonstrating dissociation of aggregates found in the untreated sample (b) . (f) Control sample demonstrating the normal planktonic state of BL21DE3 cells treated with 0.1 μ L/mL proteinase K. (c–f) ”).
    Figure Legend Snippet: Dissociation of bacterial aggregates by proteinase K treatment. (a) The cartoon demonstrates the experimental setup using proteinase K to disperse adhesion-based bacterial aggregates. (b) Comparison of the number of aggregates found in samples expressing eCPX-Dockerin-Ac after treatment with proteinase K to untreated samples. * indicates that differences between samples are significant below a p-value less than 0.05. Significance was determined by a one-tailed t test with an unequal variance that returned a p-value of 0.013. (c) Control sample demonstrating normal aggregate formation by expression of eCPX-Dockerin-Ac. Red circles highlight aggregates. (d) Control sample demonstrating normal planktonic state of BL21DE3 cells. (e) An experimental sample of cells expressing eCPX-Dockerin-Ac treated with 0.1 μ L/mL proteinase K demonstrating dissociation of aggregates found in the untreated sample (b) . (f) Control sample demonstrating the normal planktonic state of BL21DE3 cells treated with 0.1 μ L/mL proteinase K. (c–f) ”).

    Techniques Used: Expressing, One-tailed Test

    9) Product Images from "Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues"

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues

    Journal: Cartilage

    doi: 10.1177/1947603517690856

    Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with
    Figure Legend Snippet: Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with

    Techniques Used: Incubation

    10) Product Images from "Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues"

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues

    Journal: Cartilage

    doi: 10.1177/1947603517690856

    Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with
    Figure Legend Snippet: Proteinase K pretreatment enhances polyP recovery. Seven nanomoles polyP samples mixed with various concentrations of fetal bovine serum (FBS) were incubated in the presence (+PK) or absence (–PK) of proteinase K (1 mg/mL), alone or together with

    Techniques Used: Incubation

    Related Articles

    Centrifugation:

    Article Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
    Article Snippet: To purify the immunoprecipitated DNA, the IPs were first subjected to proteolytic digest using proteinase K by addition of 20 μl of 20 mg/ml proteinase K (Fermentas) and 240 μl TE buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA) followed by incubation for 2 h at 37 °C. .. Next, 50 μl 5 M LiCl and 450 μl phenol/chloroform were added and vortexed for 10 min. After centrifugation for 5 min at 13,000 rpm, the water phase was transferred to a new 1.5 ml tube containing 1 ml 96% EtOH, 5 μl glycogen (Roche) and 50 μl 3 M NaOAc and precipitated at −80 °C overnight.

    Blocking Assay:

    Article Title: Adoption of conserved developmental genes in development and origin of the medusa body plan
    Article Snippet: Proteinase K digest was done for 20 min in 1 μg/ml Proteinase K (Ambion) in 1× PBS with 0.2 % Tween 20 (Sigma-Aldrich) at RT. .. Three percent Blocking reagent (Roche) and 5 % dextran sulphate (Sigma) were added to the hybridization mix.

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates. .. Activity on all other substrates was assayed by the thiobarbituric acid assay of Warren ( ) with the following modifications for a 96 well plate: Na2 SO4 and H2 SO4 were omitted from the arsenite reagent, H2 SO4 was omitted from the thiobarb reagent, the plate was heated on a dry heating block at 85°C, and dimethyl sulfoxide (DMSO) was used to stabilize the color in place of the butanol extraction.

    Real-time Polymerase Chain Reaction:

    Article Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
    Article Snippet: To purify the immunoprecipitated DNA, the IPs were first subjected to proteolytic digest using proteinase K by addition of 20 μl of 20 mg/ml proteinase K (Fermentas) and 240 μl TE buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA) followed by incubation for 2 h at 37 °C. .. The precipitated DNA was collected by centrifugation for 5 min at 13,000 rpm, washed twice with 1 ml 70% EtOH, dried and dissolved in 50 μl ddH2 O. Real-time PCR was performed according to manufacturer’s instructions using Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher Scientific, K0221).

    Incubation:

    Article Title: Adoption of conserved developmental genes in development and origin of the medusa body plan
    Article Snippet: Proteinase K digest was done for 20 min in 1 μg/ml Proteinase K (Ambion) in 1× PBS with 0.2 % Tween 20 (Sigma-Aldrich) at RT. .. The samples were incubated in the hybridization mix over night without probe at hybridization temperature (58 °C) and subsequently hybridized for 36 h with 0.25 ng/μl digoxigenin (DIG)-labelled RNA probe.

    Article Title: Host metabolism regulates growth and differentiation of Toxoplasma gondii
    Article Snippet: .. The supernatant treatments were performed as follows: i) supernatants were spun through Amicon 3 kD cutoff columns (Merck Millipore, USA) at 3,700 g until less than 50 μl volume remained. ii) Boiling: supernatants were boiled in 1.5 ml eppendorf tubes for 10 min, together with buffer control, and allowed to cool before addition to cultures. iii) Proteinase K and trypsin: supernatants were incubated with 0.1 mg/ml of proteinase K (Invitrogen) or 0.25% trypsin (Invitrogen) for 1 h at 37°C, then spun through a 3 kD cut-off column to retain the enzymes. iv) Dialysis (1 kD): supernatants were dialyzed using Spectra/Por1 kD dialysis tubing (Cole-Palmer, USA) for 24 h at 4°C into 1 L of MEM used in conversion assay. .. For the inhibitor treatment experiment, 293T cells were plated at 2 × 107 per 15cm2 dish and incubated with or without sodium oxamate for 48 h. Phloretin was added to cells 4 h prior to harvest.

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: .. To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. Silica spin column isolation was performed on proteinase K–treated samples and aliquots of Tris HCl, pH 7.5 solution was added to the eluents (final Tris concentration of 500 mM) before fluorescence was measured.

    Article Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
    Article Snippet: .. To purify the immunoprecipitated DNA, the IPs were first subjected to proteolytic digest using proteinase K by addition of 20 μl of 20 mg/ml proteinase K (Fermentas) and 240 μl TE buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA) followed by incubation for 2 h at 37 °C. .. Next, 50 μl 5 M LiCl and 450 μl phenol/chloroform were added and vortexed for 10 min. After centrifugation for 5 min at 13,000 rpm, the water phase was transferred to a new 1.5 ml tube containing 1 ml 96% EtOH, 5 μl glycogen (Roche) and 50 μl 3 M NaOAc and precipitated at −80 °C overnight.

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Spirochetes were resuspended in phosphate-buffered saline (PBS) containing 5 mM MgCl2 (PBS+Mg), 2% bovine serum albumin (BSA), with or without 0.06% (vol/vol) Triton X-100 , and were incubated with the primary antibodies described above against CaM (dilution, 1:300) or OppAIV (1:100).

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: The sialic acid concentration in the substrates was measured as amount released after overnight incubation with excess hPIV1 and -2 virus mixture (cleavable sialic acid) or Arthrobacter ureafaciens (Sigma) or Micromonospora viridifaciens neuraminidase (total sialic acid). .. A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates.

    Article Title: Specificity and stability of the Acromyrmex–Pseudonocardia symbiosis
    Article Snippet: .. Instead of the proteinase K supplied with the kit, three μL of proteinase K (Invitrogen) was added followed by > 25 min incubation at 65 °C with frequent vortexing. ..

    Article Title: Octarepeat region flexibility impacts prion function, endoproteolysis and disease manifestation
    Article Snippet: Proteinase K and PNGaseF digestion Samples were treated with 50 μg/ml of proteinase K (PK; Invitrogen) for 1 h at 37°C. .. If samples were to undergo further PNGaseF digestion, the pellets were resuspended in 15 μl of 2% SDS and heated at 100°C for 10 min. Two microlitres of 10% NP-40, 2 μl of 10× G7 Reaction Buffer and 1 μl of PNGaseF were added, and the reaction was incubated at 37°C for 2 h. The reaction was stopped by adding 6× SDS loading dye and boiling.

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: .. To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation.

    Activity Assay:

    Article Title: Antagonistic Potential of Native Trichoderma viride Strain against Potent Tea Fungal Pathogens in North East India
    Article Snippet: .. Effects of proteinase K and heat on the antifungal activity of cell free culture filtrate To assess the stability of the extracellular metabolites, 20 days old culture filtrates of strain SDRLIN1 were either treated with 0.1 mg/ml proteinase K (Thermo Scientific, Lithuania) at 37°C for 60 min or boiled for 45 min. ..

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: .. A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates. .. Activity on all other substrates was assayed by the thiobarbituric acid assay of Warren ( ) with the following modifications for a 96 well plate: Na2 SO4 and H2 SO4 were omitted from the arsenite reagent, H2 SO4 was omitted from the thiobarb reagent, the plate was heated on a dry heating block at 85°C, and dimethyl sulfoxide (DMSO) was used to stabilize the color in place of the butanol extraction.

    Expressing:

    Article Title: Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress
    Article Snippet: .. After expression was induced (“ ”), 200 µ L of culture was pipetted into a 96-well plate with the indicated concentration of Proteinase K (Thermo Fisher). .. Samples were imaged using the brightfield of an inverted Nikon Eclipse Ti microscope with a 40 × objective.

    Western Blot:

    Article Title: Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice
    Article Snippet: .. Proteinase-K and Western Blot analysis Proteinase-K-resistant aggregates were analyzed by incubating synaptosome and cytosolic brain fractions with 10 μg/mL Proteinase-K (Thermo Scientific) for 30 minutes at 37°C, followed by SDS-PAGE western blot using the Li-cor Odyssey CLx quantitative western blot imaging system. .. Syn-GFP blot signal was detected using a rabbit polyclonal antibody against GFP (Abcam, 1:2000), and data was quantified using LiCor Image Studio software.

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Whole-cell protein preparations were analyzed by SDS-PAGE and Western immunoblotting.

    Hybridization:

    Article Title: Adoption of conserved developmental genes in development and origin of the medusa body plan
    Article Snippet: Proteinase K digest was done for 20 min in 1 μg/ml Proteinase K (Ambion) in 1× PBS with 0.2 % Tween 20 (Sigma-Aldrich) at RT. .. Three percent Blocking reagent (Roche) and 5 % dextran sulphate (Sigma) were added to the hybridization mix.

    Immunoprecipitation:

    Article Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
    Article Snippet: .. To purify the immunoprecipitated DNA, the IPs were first subjected to proteolytic digest using proteinase K by addition of 20 μl of 20 mg/ml proteinase K (Fermentas) and 240 μl TE buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA) followed by incubation for 2 h at 37 °C. .. Next, 50 μl 5 M LiCl and 450 μl phenol/chloroform were added and vortexed for 10 min. After centrifugation for 5 min at 13,000 rpm, the water phase was transferred to a new 1.5 ml tube containing 1 ml 96% EtOH, 5 μl glycogen (Roche) and 50 μl 3 M NaOAc and precipitated at −80 °C overnight.

    SDS Page:

    Article Title: Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice
    Article Snippet: .. Proteinase-K and Western Blot analysis Proteinase-K-resistant aggregates were analyzed by incubating synaptosome and cytosolic brain fractions with 10 μg/mL Proteinase-K (Thermo Scientific) for 30 minutes at 37°C, followed by SDS-PAGE western blot using the Li-cor Odyssey CLx quantitative western blot imaging system. .. Syn-GFP blot signal was detected using a rabbit polyclonal antibody against GFP (Abcam, 1:2000), and data was quantified using LiCor Image Studio software.

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Whole-cell protein preparations were analyzed by SDS-PAGE and Western immunoblotting.

    Imaging:

    Article Title: Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice
    Article Snippet: .. Proteinase-K and Western Blot analysis Proteinase-K-resistant aggregates were analyzed by incubating synaptosome and cytosolic brain fractions with 10 μg/mL Proteinase-K (Thermo Scientific) for 30 minutes at 37°C, followed by SDS-PAGE western blot using the Li-cor Odyssey CLx quantitative western blot imaging system. .. Syn-GFP blot signal was detected using a rabbit polyclonal antibody against GFP (Abcam, 1:2000), and data was quantified using LiCor Image Studio software.

    Immunofluorescence:

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Accessibility to antibodies was assessed by indirect fluorescent antibody assay (IFA) microscopy as described previously ( ).

    DNA Extraction:

    Article Title: Specificity and stability of the Acromyrmex–Pseudonocardia symbiosis
    Article Snippet: Paragraph title: DNA extraction ... Instead of the proteinase K supplied with the kit, three μL of proteinase K (Invitrogen) was added followed by > 25 min incubation at 65 °C with frequent vortexing.

    Nucleic Acid Electrophoresis:

    Article Title: Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
    Article Snippet: Treatment of OMVs with Proteinase K and Ethylenediaminetetraacetic Acid (EDTA) Purified OMVs were treated with 0.1 µg/ml of proteinase K (Fermentas) for 1 h at 37°C for degradation of surface-exposed proteins in the OMVs and 0.1 M EDTA for 1 h at 37°C for disintegration of OMV membrane. .. OMV samples, including intact OMVs and proteinase K- and EDTA-treated OMVs, were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue R-250 (Bio-Rad).

    Fluorescence:

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. The recovery ratio was calculated by dividing the DAPI fluorescence of eluted samples by the non–enzyme-treated control (50 µM polyP-45 in 10 mM Tris-EDTA, pH 8.0).

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: .. A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates. .. Activity on all other substrates was assayed by the thiobarbituric acid assay of Warren ( ) with the following modifications for a 96 well plate: Na2 SO4 and H2 SO4 were omitted from the arsenite reagent, H2 SO4 was omitted from the thiobarb reagent, the plate was heated on a dry heating block at 85°C, and dimethyl sulfoxide (DMSO) was used to stabilize the color in place of the butanol extraction.

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. Silica spin column isolation was performed on proteinase K–treated samples and aliquots of Tris HCl, pH 7.5 solution was added to the eluents (final Tris concentration of 500 mM) before fluorescence was measured.

    Isolation:

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. The recovery ratio was calculated by dividing the DAPI fluorescence of eluted samples by the non–enzyme-treated control (50 µM polyP-45 in 10 mM Tris-EDTA, pH 8.0).

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. Silica spin column isolation was performed on proteinase K–treated samples and aliquots of Tris HCl, pH 7.5 solution was added to the eluents (final Tris concentration of 500 mM) before fluorescence was measured.

    Microscopy:

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Accessibility to antibodies was assessed by indirect fluorescent antibody assay (IFA) microscopy as described previously ( ).

    Purification:

    Article Title: Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
    Article Snippet: .. Treatment of OMVs with Proteinase K and Ethylenediaminetetraacetic Acid (EDTA) Purified OMVs were treated with 0.1 µg/ml of proteinase K (Fermentas) for 1 h at 37°C for degradation of surface-exposed proteins in the OMVs and 0.1 M EDTA for 1 h at 37°C for disintegration of OMV membrane. .. OMV samples, including intact OMVs and proteinase K- and EDTA-treated OMVs, were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue R-250 (Bio-Rad).

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: Bovine fetuin (bFetuin) was purified from fetal calf serum (Sigma) by the method of Spiro ( ). .. A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates.

    Staining:

    Article Title: Acinetobacter baumannii Outer Membrane Vesicles Elicit a Potent Innate Immune Response via Membrane Proteins
    Article Snippet: Treatment of OMVs with Proteinase K and Ethylenediaminetetraacetic Acid (EDTA) Purified OMVs were treated with 0.1 µg/ml of proteinase K (Fermentas) for 1 h at 37°C for degradation of surface-exposed proteins in the OMVs and 0.1 M EDTA for 1 h at 37°C for disintegration of OMV membrane. .. OMV samples, including intact OMVs and proteinase K- and EDTA-treated OMVs, were separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and stained with Coomassie brilliant blue R-250 (Bio-Rad).

    Chromatin Immunoprecipitation:

    Article Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
    Article Snippet: Paragraph title: 2.12. Chromatin immunoprecipitation ... To purify the immunoprecipitated DNA, the IPs were first subjected to proteolytic digest using proteinase K by addition of 20 μl of 20 mg/ml proteinase K (Fermentas) and 240 μl TE buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA) followed by incubation for 2 h at 37 °C.

    Article Title: Telomere Regulation During the Cell Cycle in Fission Yeast
    Article Snippet: Paragraph title: 2.2 Chromatin Immunoprecipitation Analysis ... Proteinase K: Dissolve Proteinase K (Invitrogen) at 10 mg/ mL in 10 mM Tris–HCl pH 7.5, 20 mM calcium chloride, 50 % glycerol.

    In Situ Hybridization:

    Article Title: Adoption of conserved developmental genes in development and origin of the medusa body plan
    Article Snippet: Paragraph title: In situ hybridization ... Proteinase K digest was done for 20 min in 1 μg/ml Proteinase K (Ambion) in 1× PBS with 0.2 % Tween 20 (Sigma-Aldrich) at RT.

    Software:

    Article Title: Curcumin Treatment Improves Motor Behavior in α-Synuclein Transgenic Mice
    Article Snippet: Proteinase-K and Western Blot analysis Proteinase-K-resistant aggregates were analyzed by incubating synaptosome and cytosolic brain fractions with 10 μg/mL Proteinase-K (Thermo Scientific) for 30 minutes at 37°C, followed by SDS-PAGE western blot using the Li-cor Odyssey CLx quantitative western blot imaging system. .. Syn-GFP blot signal was detected using a rabbit polyclonal antibody against GFP (Abcam, 1:2000), and data was quantified using LiCor Image Studio software.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates. .. Virus, buffer, and substrate were mixed to a starting volume of 30 μl in a 96-well flat-bottom enzyme-linked immunosorbent assay (ELISA) plate (Microlon; Greiner Bio-One).

    In Situ:

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: .. To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Whole-cell protein preparations were analyzed by SDS-PAGE and Western immunoblotting.

    Acid Assay:

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates. .. Activity on all other substrates was assayed by the thiobarbituric acid assay of Warren ( ) with the following modifications for a 96 well plate: Na2 SO4 and H2 SO4 were omitted from the arsenite reagent, H2 SO4 was omitted from the thiobarb reagent, the plate was heated on a dry heating block at 85°C, and dimethyl sulfoxide (DMSO) was used to stabilize the color in place of the butanol extraction.

    Concentration Assay:

    Article Title: Host metabolism regulates growth and differentiation of Toxoplasma gondii
    Article Snippet: Cells (293T and NIH3T3) were extensively washed prior to incubation at a concentration of 3 × 107 per ml in Hank’s Balanced Salt Solution without calcium or magnesium, pH 7.0 (HBSS; Invitrogen) for 6 h in a 12 well tissue culture dish after which supernatants, together with a mock cell control, were collected and stored at 4°C. .. The supernatant treatments were performed as follows: i) supernatants were spun through Amicon 3 kD cutoff columns (Merck Millipore, USA) at 3,700 g until less than 50 μl volume remained. ii) Boiling: supernatants were boiled in 1.5 ml eppendorf tubes for 10 min, together with buffer control, and allowed to cool before addition to cultures. iii) Proteinase K and trypsin: supernatants were incubated with 0.1 mg/ml of proteinase K (Invitrogen) or 0.25% trypsin (Invitrogen) for 1 h at 37°C, then spun through a 3 kD cut-off column to retain the enzymes. iv) Dialysis (1 kD): supernatants were dialyzed using Spectra/Por1 kD dialysis tubing (Cole-Palmer, USA) for 24 h at 4°C into 1 L of MEM used in conversion assay.

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. The recovery ratio was calculated by dividing the DAPI fluorescence of eluted samples by the non–enzyme-treated control (50 µM polyP-45 in 10 mM Tris-EDTA, pH 8.0).

    Article Title: Engineered Stochastic Adhesion Between Microbes as a Protection Mechanism Against Environmental Stress
    Article Snippet: .. After expression was induced (“ ”), 200 µ L of culture was pipetted into a 96-well plate with the indicated concentration of Proteinase K (Thermo Fisher). .. Samples were imaged using the brightfield of an inverted Nikon Eclipse Ti microscope with a 40 × objective.

    Article Title: Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿Fixation of Oligosaccharides to a Surface May Increase the Susceptibility to Human Parainfluenza Virus 1, 2, or 3 Hemagglutinin-Neuraminidase ▿ †
    Article Snippet: The sialic acid concentration in the substrates was measured as amount released after overnight incubation with excess hPIV1 and -2 virus mixture (cleavable sialic acid) or Arthrobacter ureafaciens (Sigma) or Micromonospora viridifaciens neuraminidase (total sialic acid). .. A proteinase K digest of bFetuin (fetuin peptides) was prepared by incubating a solution of 40 mg/ml bFetuin and 3 to 4 mg/ml proteinase K (Invitrogen) in neuraminidase buffer (50 mM Na acetate, 4 mM CaCl2 , pH 5.5) at 50°C for 2 h. Neuraminidase activity on MUN was assayed by the fluorescence method of Potier et al. ( ) adapted for 96-well plates.

    Article Title: Simple Silica Column–Based Method to Quantify Inorganic Polyphosphates in Cartilage and Other Tissues
    Article Snippet: To determine if proteinase K is necessary to extract polyP from protein containing tissues/solutions, 7 nmol polyP-45 (50 µM) was mixed with 20% or 50% (v/v) FBS and incubated in the presence or absence of proteinase K (1 mg/mL, Life Technologies, Burlington, Ontario, Canada) in 10 mM Tris, pH 8.0, for 4 hours at 56°C with periodic agitation. .. Silica spin column isolation was performed on proteinase K–treated samples and aliquots of Tris HCl, pH 7.5 solution was added to the eluents (final Tris concentration of 500 mM) before fluorescence was measured.

    DNA Purification:

    Article Title: Specificity and stability of the Acromyrmex–Pseudonocardia symbiosis
    Article Snippet: DNA extraction The dissected cuticular fragments were placed individually in sterile 2-mL screw lid tubes with 0.1-mm glass beads (MO BIO laboratories, Inc.), and DNA was extracted with a MasterPure DNA purification kit (Epicentre Technologies), which targets Gram-negative and Gram-positive bacteria with about equal efficiency (Rantakokko-Jalava & Jalava ). .. Instead of the proteinase K supplied with the kit, three μL of proteinase K (Invitrogen) was added followed by > 25 min incubation at 65 °C with frequent vortexing.

    Lysis:

    Article Title: SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination
    Article Snippet: Next, the beads were washed in 1 ml ChIP lysis buffer and transferred to a new 2 ml tube. .. To purify the immunoprecipitated DNA, the IPs were first subjected to proteolytic digest using proteinase K by addition of 20 μl of 20 mg/ml proteinase K (Fermentas) and 240 μl TE buffer (10 mM Tris–HCl pH 7.5, 1 mM EDTA) followed by incubation for 2 h at 37 °C.

    Article Title: Specificity and stability of the Acromyrmex–Pseudonocardia symbiosis
    Article Snippet: In short, 300 μL tissue lysis buffer was added and the bacterial membranes disrupted in a FastPrep machine for 45 s at 4.5 speed. .. Instead of the proteinase K supplied with the kit, three μL of proteinase K (Invitrogen) was added followed by > 25 min incubation at 65 °C with frequent vortexing.

    Chick Chorioallantoic Membrane Assay:

    Article Title: Probing the Borrelia burgdorferi Surface Lipoprotein Secretion Pathway Using a Conditionally Folding Protein Domain ▿
    Article Snippet: To assess the surface exposure of a protein by its accessibility to proteinase K, intact B. burgdorferi cells were harvested, washed, and treated in situ with 200 μg/ml proteinase K (Invitrogen) as described previously ( ). .. Spirochetes were resuspended in phosphate-buffered saline (PBS) containing 5 mM MgCl2 (PBS+Mg), 2% bovine serum albumin (BSA), with or without 0.06% (vol/vol) Triton X-100 , and were incubated with the primary antibodies described above against CaM (dilution, 1:300) or OppAIV (1:100).

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    Thermo Fisher proteinase k
    Proteinase K, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k/product/Thermo Fisher
    Average 90 stars, based on 727 article reviews
    Price from $9.99 to $1999.99
    proteinase k - by Bioz Stars, 2020-01
    90/100 stars
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    This is the Proteinase K found in the MagMAX 96 DNA Multi Sample Kit Cat Nos 4413020 4413021 4413022 made available separately for applications that require more proteinase K enzyme
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    Description USB Proteinase K is a highly active and stable endopeptidase used in a wide range of applications purified from the fungus T album It cleaves peptide bonds mainly following
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