proteinase k (Qiagen)

Qiagen is a verified supplier

Qiagen manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Name:

Puregene Proteinase K

Description:

For purification of archive quality DNA from a wide range of sample types Kit contents 650 l Puregene Proteinase K Benefits Archive quality DNA for long term storage Reproducible DNA purification Convenient scalable purification proced

Catalog Number:

158918

Price:

83.5

Category:

Puregene Accessories

Buy from Supplier

Name:

QIAGEN Proteinase K

Description:

For protease digestion during DNA and RNA preparation Kit contents Qiagen Proteinase K 2mL 600mAU mL Activity Ready to use Solution For Protease Digestion During DNA and RNA Preparation Offer Broad Substrate Specificity with High Activity for a Wide Range of Reaction Conditions Used in Most DNA and RNA Isolation

Catalog Number:

19131

Price:

96.3

Category:

QIAGEN Proteinase K

Buy from Supplier

Structured Review

Qiagen proteinase k

proteinase k - by Bioz Stars, 2020-01

99/100 stars

Related Products / Commonly Used Together

edta
sds
rnase a
pbs
nacl
tris-hcl
tris
rnase
rna
dnase i
antigen retrieval
triton x-100
genomic dna
dna extraction
qiaquick pcr purification kit

Images

1) Product Images from "Pellicle formation in Shewanella oneidensis"

Article Title: Pellicle formation in Shewanella oneidensis

Journal: BMC Microbiology

doi: 10.1186/1471-2180-10-291

EPS analysis . (A) Effects of proteinase K on pellicle formation and developed pellicles. Upper-panel, pellicle formation of the WT in static LB, in which the proteinase K was added at inoculation to 100 mg/ml (final concentration). Lower panel, developed pellicles of the WT (48 h after inoculation) were treated with 100 mg/ml (final concentration). (B) TLC analysis of monosaccharide in pellicles and supernatants. P and S represent pellicle and supernatant, respectively. Man, gal, and glu represent mannose, galactose, and glucose, respectively. Supernatants of the aggA mutant culture were included in the analysis.

Figure Legend Snippet: EPS analysis . (A) Effects of proteinase K on pellicle formation and developed pellicles. Upper-panel, pellicle formation of the WT in static LB, in which the proteinase K was added at inoculation to 100 mg/ml (final concentration). Lower panel, developed pellicles of the WT (48 h after inoculation) were treated with 100 mg/ml (final concentration). (B) TLC analysis of monosaccharide in pellicles and supernatants. P and S represent pellicle and supernatant, respectively. Man, gal, and glu represent mannose, galactose, and glucose, respectively. Supernatants of the aggA mutant culture were included in the analysis.

Techniques Used: Concentration Assay, Thin Layer Chromatography, Mutagenesis

Centrifugation:

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: The spheroplasts were collected by centrifugation at 2000 × g for 5 min and resuspended in 1 ml of MNase digestion buffer (50 mM Tris–HCl, pH 7.6; 1 mM CaCl2 ; 0.2% (v/v) Triton X-100) containing 1 × protease inhibitor (Nacalai Tesque). .. The reaction mixture was then supplemented with 100 μl of 10% (w/v) SDS and 25 μl of 20 mg/ml Protease K (Qiagen), and incubated at 50°C for 1 h. Following phenol and phenol/chloroform extraction, nucleic acids were collected by isopropanol precipitation.

Luciferase:

Article Title: Blood-brain barrier shuttle peptides enhance AAV transduction in the brain after systemic administration
Article Snippet: The different minced tissues and the blood collected at different time points were treated with Protease K, and the total gDNA was isolated using the DNeasy blood & tissue kit (Qiagen, USA). .. The luciferase gene was measured by qPCR assay.

Real-time Polymerase Chain Reaction:

Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
Article Snippet: ChIP DNA were treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen). .. The purified ChIP DNA was quantified by real-time PCR using the iQ SYBR Green Supermix (Bio-Rad).

Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
Article Snippet: ChIP DNA was treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen). .. The purified ChIP DNA was quantified by real-time PCR using the iQ SYBR Green Supermix (Bio-Rad).

Article Title: ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4
Article Snippet: Following reverse-cross-linking, the samples were treated with RNase and Protease K, and the DNA was purified using the Qiagen PCR Purification Kit. .. 2 μL ChIP product was used for each qPCR reaction.

Incubation:

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: .. 3′-Azide modification of ssDNA To 50 μl of solution that contained 1 × TdT Buffer, 20 μM Az-ddNTP, and ssDNA indicated in each experiment, 60 units of buffer-exchanged TdT enzyme were added, and the mixture was incubated at 37°C for 1 h. After heat inactivation of the TdT by incubation at 70°C for 10 min, 1 μl of 20 mg/ml Protease K (Qiagen, Hilden, Germany) was added and incubated at 50°C for 10 min. After heat inactivation of the protease at 95°C for 5 min, the DNA was purified using the double Sera-Mag procedure detailed above with an elution volume of 7 μl. .. Click ligation of 5′-ethynyl adaptor to 3′-azide-modified ssDNA The Cu(I) catalyst solution was prepared fresh just prior to use by combining equal volumes of a solution of 50 mM CuSO4 (Wako Pure Chemicals Co., Osaka, Japan) and 500 mM tris(3-hydroxypropyltriazolyl-methyl)amine (THPTA) (Sigma-Aldrich Co. LLC, St Louis, MO, USA) with a solution of 100 mM sodium ascorbate (Sigma-Aldrich) and 100 mM aminoguanidine (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan).

Article Title: Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
Article Snippet: .. The samples were Proteinase K treated for 1 h at 55°C with moderate shaking using 45 μl of Proteinase K (20 mg ml-1 ; Qiagen) and treated with 4 μl RNase A and incubated for 10 min at 65°C. .. The filters were removed using sterile needles, 130 μl AP2 buffer (Qiagen Plant Minikit) was added to each tube, and samples were then vortexed and incubated on ice for 10 min. To pellet the precipitates and beads, the tubes were centrifuged for 5 min at 14 000 rpm at 4°C, and the supernatant was transferred to 2 ml sample tubes and placed in the QIAcube for further extraction steps according to the manufacturer’s protocol.

Article Title: Arid1a has context-dependent oncogenic and tumor suppressor functions in liver cancer
Article Snippet: After overnight incubation, protein A/G Dynabeads (Invitrogen) were added to the ChIP reactions and incubated for 4 additional hr at 4 °C to collect the immunoprecipitated chromatin. .. ChIP DNA was treated with RNaseA (5 g/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
Article Snippet: After overnight incubation, protein A or G Dynabeads (Invitrogen) were added to the ChIP reactions and incubated for four additional hours at 4°C to collect the immunoprecipitated chromatin. .. ChIP DNA were treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

Article Title: Biomarker significance of plasma and tumor miR-21, miR-221, and miR-106a in osteosarcoma
Article Snippet: Proteinase K unmasking was done by treating with 20 μg/mL Proteinase K (Exiqon) for 10 min at 37°C. .. They were prepared in the hybridization buffer containing yeast tRNA, formamide, heparin, and 0.1 % Tween 20 and incubated with the tissue spots for 1 hour covered with HybriSlip (Electron Microscopy Sciences, Hatfield, PA).

Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
Article Snippet: After overnight incubation, protein A or G Dynabeads (Invitrogen) were added to the ChIP reactions and incubated for four additional hours at 4 °C to collect the immunoprecipitated chromatin. .. ChIP DNA was treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: .. The reaction mixture was then supplemented with 100 μl of 10% (w/v) SDS and 25 μl of 20 mg/ml Protease K (Qiagen), and incubated at 50°C for 1 h. Following phenol and phenol/chloroform extraction, nucleic acids were collected by isopropanol precipitation. .. Any contaminating RNA was digested with 250 units of RNase If (New England BioLabs) and the 3′-phosphate of the MNase-treated DNA was removed with 5 units of shrimp alkaline phosphatase (Takara Bio Inc.).

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: .. To 50 μl of solution that contained 1 × TdT Buffer, 20 μM Az-ddNTP, and ssDNA indicated in each experiment, 60 units of buffer-exchanged TdT enzyme were added, and the mixture was incubated at 37°C for 1 h. After heat inactivation of the TdT by incubation at 70°C for 10 min, 1 μl of 20 mg/ml Protease K (Qiagen, Hilden, Germany) was added and incubated at 50°C for 10 min. After heat inactivation of the protease at 95°C for 5 min, the DNA was purified using the double Sera-Mag procedure detailed above with an elution volume of 7 μl. .. The Cu(I) catalyst solution was prepared fresh just prior to use by combining equal volumes of a solution of 50 mM CuSO4 (Wako Pure Chemicals Co., Osaka, Japan) and 500 mM tris(3-hydroxypropyltriazolyl-methyl)amine (THPTA) (Sigma-Aldrich Co. LLC, St Louis, MO, USA) with a solution of 100 mM sodium ascorbate (Sigma-Aldrich) and 100 mM aminoguanidine (Tokyo Chemical Industry Co., Ltd., Tokyo, Japan).

Article Title: ClearCode34: A Prognostic Risk Predictor for Localized Clear Cell Renal Cell Carcinoma
Article Snippet: Pellets were suspended in 10 mM 2-(N-morpholino) ethanesulfonic acid pH 6.5 or Proteinase K digest buffer (Qiagen Gaithersburg Inc, Gaithersburg, MD, USA) with 0.5% SDS and 5 μl Proteinase K (20 mg/ml). .. Suspensions were incubated (55°C), Proteinase K inactivated (80°C), and supernatant collected.

Article Title: Pellicle formation in Shewanella oneidensis
Article Snippet: Proteinase K and DNase I treatment of S. oneidensis pellicles S. oneidensis was statically cultured in LB broth with the addition of proteinase K (0 μg/mL, 100 μg/mL, and 500 μg/mL) or DNase I (Qiagen, 0U/mL, 100U/mL, 500U/mL and 1000U/mL) for 3 days [ ]. .. 2-day old pellicles were rinsed with 20 mM Tris-HCl (pH = 8.0) and incubated in the same buffer supplemented with proteinase K at 37°C for 2 days.

Formalin-fixed Paraffin-Embedded:

Article Title: ClearCode34: A Prognostic Risk Predictor for Localized Clear Cell Renal Cell Carcinoma
Article Snippet: Paragraph title: 2.3. Formalin-fixed paraffin-embedded sample preparation ... Pellets were suspended in 10 mM 2-(N-morpholino) ethanesulfonic acid pH 6.5 or Proteinase K digest buffer (Qiagen Gaithersburg Inc, Gaithersburg, MD, USA) with 0.5% SDS and 5 μl Proteinase K (20 mg/ml).

Modification:

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: Paragraph title: 3′-Azide modification of ssDNA ... To 50 μl of solution that contained 1 × TdT Buffer, 20 μM Az-ddNTP, and ssDNA indicated in each experiment, 60 units of buffer-exchanged TdT enzyme were added, and the mixture was incubated at 37°C for 1 h. After heat inactivation of the TdT by incubation at 70°C for 10 min, 1 μl of 20 mg/ml Protease K (Qiagen, Hilden, Germany) was added and incubated at 50°C for 10 min. After heat inactivation of the protease at 95°C for 5 min, the DNA was purified using the double Sera-Mag procedure detailed above with an elution volume of 7 μl.

Hybridization:

Article Title: Biomarker significance of plasma and tumor miR-21, miR-221, and miR-106a in osteosarcoma
Article Snippet: Paragraph title: miRNA in-situ hybridization (ISH) ... Proteinase K unmasking was done by treating with 20 μg/mL Proteinase K (Exiqon) for 10 min at 37°C.

Electron Microscopy:

Protease Inhibitor:

Cell Culture:

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: The cultured yeast cells were collected with centrifugation at 2,000 × g for 5 min, and re-suspended in 1 ml of spheroplast buffer that contained 1 M sorbitol; 50 mM Tris–HCl, pH 7.5; and 1 mM β-mercaptoethanol. .. The reaction mixture was then supplemented with 100 μl of 10% (w/v) SDS and 25 μl of 20 mg/ml Protease K (Qiagen), and incubated at 50°C for 1 h. Following phenol and phenol/chloroform extraction, nucleic acids were collected by isopropanol precipitation.

Article Title: Pellicle formation in Shewanella oneidensis
Article Snippet: .. Proteinase K and DNase I treatment of S. oneidensis pellicles S. oneidensis was statically cultured in LB broth with the addition of proteinase K (0 μg/mL, 100 μg/mL, and 500 μg/mL) or DNase I (Qiagen, 0U/mL, 100U/mL, 500U/mL and 1000U/mL) for 3 days [ ]. .. 2-day old pellicles were rinsed with 20 mM Tris-HCl (pH = 8.0) and incubated in the same buffer supplemented with proteinase K at 37°C for 2 days.

Polymerase Chain Reaction:

Article Title: Triazole linking for preparation of a next-generation sequencing library from single-stranded DNA
Article Snippet: The reaction mixture was then supplemented with 100 μl of 10% (w/v) SDS and 25 μl of 20 mg/ml Protease K (Qiagen), and incubated at 50°C for 1 h. Following phenol and phenol/chloroform extraction, nucleic acids were collected by isopropanol precipitation. .. The final DNA purification was accomplished using a QIAQuick PCR purification kit (Qiagen) according to the manufacturer's instructions.

Article Title: ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4
Article Snippet: .. Following reverse-cross-linking, the samples were treated with RNase and Protease K, and the DNA was purified using the Qiagen PCR Purification Kit. .. 2 μL ChIP product was used for each qPCR reaction.

Sonication:

Article Title: Arid1a has context-dependent oncogenic and tumor suppressor functions in liver cancer
Article Snippet: Chromatin was sonicated to around 500 bp in buffer 0 (10 mM Tris-HCl, 1 mM EDTA, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.25% sarkosyl, pH 8.0) with 0.3 M NaCl. .. ChIP DNA was treated with RNaseA (5 g/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
Article Snippet: Chromatin was sonicated to around 500 bp in RIPA buffer (10 mM Tris-HCl, 1 mM EDTA, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.25% sarkosyl, pH 8.0) with 0.3 M NaCl. .. ChIP DNA were treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
Article Snippet: Chromatin was sonicated to around 500 bp in RIPA buffer (10 mM Tris-HCl, 1 mM EDTA, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.25% sarkosyl, pH 8.0) with 0.3 M NaCl. .. ChIP DNA was treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

Article Title: ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4
Article Snippet: The sonicated chromatin was immunoprecipitated overnight at 4°C with J1 antibody , Poll II, or same amount of IgG control. .. Following reverse-cross-linking, the samples were treated with RNase and Protease K, and the DNA was purified using the Qiagen PCR Purification Kit.

ChIP-sequencing:

Article Title: Arid1a has context-dependent oncogenic and tumor suppressor functions in liver cancer
Article Snippet: Paragraph title: ChIP-Seq ... ChIP DNA was treated with RNaseA (5 g/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen).

DNA Extraction:

Article Title: Identification of Associations between Bacterioplankton and Photosynthetic Picoeukaryotes in Coastal Waters
Article Snippet: Paragraph title: DNA Extraction ... The samples were Proteinase K treated for 1 h at 55°C with moderate shaking using 45 μl of Proteinase K (20 mg ml-1 ; Qiagen) and treated with 4 μl RNase A and incubated for 10 min at 65°C.

Article Title: Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq
Article Snippet: Paragraph title: Genomic DNA isolation ... 19101) Proteinase K (Qiagen, cat.no.

Isolation:

Article Title: Blood-brain barrier shuttle peptides enhance AAV transduction in the brain after systemic administration
Article Snippet: .. The different minced tissues and the blood collected at different time points were treated with Protease K, and the total gDNA was isolated using the DNeasy blood & tissue kit (Qiagen, USA). .. The luciferase gene was measured by qPCR assay.

Purification:

Article Title: Arid1a has context-dependent oncogenic and tumor suppressor functions in liver cancer
Article Snippet: .. ChIP DNA was treated with RNaseA (5 g/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen). .. The purified ChIP DNA was used for library preparation.

Article Title: CPS1 maintains pyrimidine pools and DNA synthesis in KRAS/LKB1-mutant lung cancer cells
Article Snippet: .. ChIP DNA were treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen). .. The purified ChIP DNA was quantified by real-time PCR using the iQ SYBR Green Supermix (Bio-Rad).

Article Title: Dissecting super-enhancer hierarchy based on chromatin interactions
Article Snippet: .. ChIP DNA was treated with RNaseA (5 μg/ml) and protease K (0.2 mg/ml), and purified using QIAquick Spin Columns (Qiagen). .. The purified ChIP DNA was quantified by real-time PCR using the iQ SYBR Green Supermix (Bio-Rad).

Chloramphenicol Acetyltransferase Assay:

Article Title: Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq
Article Snippet: .. 19101) Proteinase K (Qiagen, cat.no. .. IDTE pH 8.0 (1× TE Solution) (Integrated DNA Technologies, cat.no.

Chromatin Immunoprecipitation:

Article Title: ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4
Article Snippet: Paragraph title: Chromatin Immunoprecipitation ... Following reverse-cross-linking, the samples were treated with RNase and Protease K, and the DNA was purified using the Qiagen PCR Purification Kit.

In Situ Hybridization:

SYBR Green Assay:

Negative Control:

Article Title: Biomarker significance of plasma and tumor miR-21, miR-221, and miR-106a in osteosarcoma
Article Snippet: Proteinase K unmasking was done by treating with 20 μg/mL Proteinase K (Exiqon) for 10 min at 37°C. .. The TMAs were fixed with 4% formaldehyde in PBS followed by EDC (1-Ethyl-3-(3-dimethylaminopropyl carbodiimide). miR-21, U6, and negative control (scrambled miRNA) probes with LNA-modified and 5′- and 3′-DIG-labeled oligonucleotides (Exiqon) were tested in each experiment.

Sample Prep:

In Situ:

Immunoprecipitation:

DNA Purification:

Lysis:

Article Title: Defining CRISPR-Cas9 genome-wide nuclease activities with CIRCLE-seq
Article Snippet: 158667) Cell Lysis Solution (Qiagen) supplied with Gentra Puregene Kit Protein Precipitation Solution (Qiagen) supplied with Gentra Puregene Kit DNA Hydration Solution (Qiagen) supplied with Gentra Puregene Kit Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, cat.no. ) .. 19101) Proteinase K (Qiagen, cat.no.