proteinase k solution  (Thermo Fisher)


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    Thermo Fisher proteinase k solution
    Proteinase K Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k solution/product/Thermo Fisher
    Average 99 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    proteinase k solution - by Bioz Stars, 2020-04
    99/100 stars

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    DNA Extraction:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: Paragraph title: 2.4. DNA isolation ... Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl.

    Centrifugation:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl. .. Sperm was isolated from epididymis using the swim-out technique and pelleted by centrifugation at 300× g for 5 min. Genomic DNA was then isolated using the salting-out method described above after an overnight incubation in ATL lysis buffer containing 0.55 mg/ml proteinase K solution and 30 mM DTT.

    Recombinant:

    Article Title: Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43.
    Article Snippet: Paragraph title: Construction of the recombinant baculovirus ... After incubation time, 2 L of Proteinase K solution (Invitrogen) was added to the reaction, and incubated 10 min at 37 • C. Lipid mediated transfection of the Sf21 cells was performed with Cellfectin ® Reagent (Invitrogen) in six well plates.

    Isolation:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: Genomic DNA from Hepatocytes and NPCs was isolated using the salting-out method. .. Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl.

    Transfection:

    Article Title: Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43.
    Article Snippet: .. After incubation time, 2 L of Proteinase K solution (Invitrogen) was added to the reaction, and incubated 10 min at 37 • C. Lipid mediated transfection of the Sf21 cells was performed with Cellfectin ® Reagent (Invitrogen) in six well plates. ..

    Mouse Assay:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: Genomic DNA from tails of 3-week-old mice was extracted using KAPA Mouse Genotyping Kit (KAPA Biosystems, Wilmington, MA). .. Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl.

    Concentration Assay:

    Article Title: Investigation into the cellular origins of posterior regeneration in the annelid Capitella teleta. Investigation into the cellular origins of posterior regeneration in the annelid Capitella teleta
    Article Snippet: Juveniles collected for the 0 hpa time point were then exposed to BrdU (Sigma 858811) at a final concentration of 0.1 mg/mL for 1 h, before being placed in 1:1 0.37 mol/L MgCl2 :FSW solution for 15 min to immobilize them, and then fixed in 3.7% PFA:FSW overnight at 4°C. .. Following fixation, animals were rinsed several times with PBS and digested with 0.01 mg/mL Proteinase K (Invitrogen 25530049) in PBS for 5 min. After this incubation, the Proteinase K solution was removed and animals were re‐fixed in 3.7% PFA:PBS for 10 min.

    Incubation:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: .. Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl. ..

    Article Title: Investigation into the cellular origins of posterior regeneration in the annelid Capitella teleta. Investigation into the cellular origins of posterior regeneration in the annelid Capitella teleta
    Article Snippet: .. Following fixation, animals were rinsed several times with PBS and digested with 0.01 mg/mL Proteinase K (Invitrogen 25530049) in PBS for 5 min. After this incubation, the Proteinase K solution was removed and animals were re‐fixed in 3.7% PFA:PBS for 10 min. ..

    Article Title: Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43.
    Article Snippet: .. After incubation time, 2 L of Proteinase K solution (Invitrogen) was added to the reaction, and incubated 10 min at 37 • C. Lipid mediated transfection of the Sf21 cells was performed with Cellfectin ® Reagent (Invitrogen) in six well plates. ..

    DNA Purification:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: Genomic DNA of different organs was isolated using the Maxwell®16 Mouse Tail DNA Purification kit (Promega, Madison, WI). .. Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl.

    Salting Out:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: Genomic DNA from Hepatocytes and NPCs was isolated using the salting-out method. .. Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl.

    Lysis:

    Article Title: Double-strand break repair plays a role in repeat instability in a Fragile X mouse model.
    Article Snippet: .. Briefly, cells were resuspended in ATL lysis buffer (Qiagen, Valencia, CA) with 0.55 mg/ml proteinase K solution (Invitrogen, Carlsbad, CA) and incubated at 55°C overnight before the addition of 1.5 M NaCl. ..

    Purification:

    Article Title: Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43.
    Article Snippet: Construction of the recombinant baculovirus Recombination reaction was performed 18 h at room temperature in a microcentrifuge tube containing 100 ng (2 L) of the purified entry vector, 300 ng (10 L) of the BaculoDirect TM Lin-ear DNA, 4 L of 5× LR Clonase TM Reaction Buffer and 4 L of LR Clonase TM Enzyme mix. .. After incubation time, 2 L of Proteinase K solution (Invitrogen) was added to the reaction, and incubated 10 min at 37 • C. Lipid mediated transfection of the Sf21 cells was performed with Cellfectin ® Reagent (Invitrogen) in six well plates.

    Plasmid Preparation:

    Article Title: Baculovirus expression of HCoV-OC43 nucleocapsid protein and development of a Western blot assay for detection of human antibodies against HCoV-OC43.
    Article Snippet: Construction of the recombinant baculovirus Recombination reaction was performed 18 h at room temperature in a microcentrifuge tube containing 100 ng (2 L) of the purified entry vector, 300 ng (10 L) of the BaculoDirect TM Lin-ear DNA, 4 L of 5× LR Clonase TM Reaction Buffer and 4 L of LR Clonase TM Enzyme mix. .. After incubation time, 2 L of Proteinase K solution (Invitrogen) was added to the reaction, and incubated 10 min at 37 • C. Lipid mediated transfection of the Sf21 cells was performed with Cellfectin ® Reagent (Invitrogen) in six well plates.

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    Thermo Fisher proteinase k solution
    Identification of ECM-embedded MBVs. ( A ) TEM imaging of MBVs identified in a UBM sheet stained positive with osmium (left panel), pepsin-treated UBM (middle panel), or proteinase K–treated UBM (right panel). ( B ) TEM imaging of MBVs identified in proteinase K–treated ECM from three commercial and three laboratory-produced scaffolds. Scale bars, 100 nm. ( C ) Validation of MBV size was measured with NanoSight. ( D ) Western blot analysis was performed on four exosomal surface markers: CD63, CD81, CD9, and Hsp70. Expression levels were not detectable as compared to porcine serum, human serum, and human bone marrow–derived mesenchymal stem cell controls. ( E ) MBV protein cargo signature was different between MBVs and hMSCs as evaluated using SDS-PAGE and silver stain imaging.
    Proteinase K Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k solution/product/Thermo Fisher
    Average 99 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    proteinase k solution - by Bioz Stars, 2020-04
    99/100 stars
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    Identification of ECM-embedded MBVs. ( A ) TEM imaging of MBVs identified in a UBM sheet stained positive with osmium (left panel), pepsin-treated UBM (middle panel), or proteinase K–treated UBM (right panel). ( B ) TEM imaging of MBVs identified in proteinase K–treated ECM from three commercial and three laboratory-produced scaffolds. Scale bars, 100 nm. ( C ) Validation of MBV size was measured with NanoSight. ( D ) Western blot analysis was performed on four exosomal surface markers: CD63, CD81, CD9, and Hsp70. Expression levels were not detectable as compared to porcine serum, human serum, and human bone marrow–derived mesenchymal stem cell controls. ( E ) MBV protein cargo signature was different between MBVs and hMSCs as evaluated using SDS-PAGE and silver stain imaging.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Identification of ECM-embedded MBVs. ( A ) TEM imaging of MBVs identified in a UBM sheet stained positive with osmium (left panel), pepsin-treated UBM (middle panel), or proteinase K–treated UBM (right panel). ( B ) TEM imaging of MBVs identified in proteinase K–treated ECM from three commercial and three laboratory-produced scaffolds. Scale bars, 100 nm. ( C ) Validation of MBV size was measured with NanoSight. ( D ) Western blot analysis was performed on four exosomal surface markers: CD63, CD81, CD9, and Hsp70. Expression levels were not detectable as compared to porcine serum, human serum, and human bone marrow–derived mesenchymal stem cell controls. ( E ) MBV protein cargo signature was different between MBVs and hMSCs as evaluated using SDS-PAGE and silver stain imaging.

    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific.

    Techniques: Transmission Electron Microscopy, Imaging, Staining, Produced, Western Blot, Expressing, Derivative Assay, SDS Page, Silver Staining

    Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Enzymatic digestion of decellularized ECM scaffolds releases small RNA molecules. ( A ) Nucleic acid extracted from untreated UBM (no digest) and pepsin-, proteinase K–, or collagenase-treated UBM was exposed to RNase A, DNase I, or no-nuclease treatment (control). ( B ) Electropherogram depicting the small RNA pattern of nucleic acid in fluorescence units (FU) before (top panel) and after (bottom panel) DNase I treatment. ( C ) Electropherogram depicting small RNA pattern from the indicated samples in FU. ( D ) A subset of nucleic molecules in biologic scaffolds is protected from nuclease degradation.

    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific.

    Techniques: Fluorescence

    Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A  to  C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD;  n  = 3 isolations per sample.

    Journal: Science Advances

    Article Title: Matrix-bound nanovesicles within ECM bioscaffolds

    doi: 10.1126/sciadv.1600502

    Figure Lengend Snippet: Comparison of nucleic acid concentration from UBM, SIS, or dermis and their commercially available equivalents. ( A to C ) Concentration of total nucleic acid and dsDNA per milligram dry weight of ECM scaffold from untreated (control) and proteinase K– or collagenase-treated samples of (A) UBM and ACell MatriStem (porcine UBM), (B) SIS and Cook Biotech Biodesign (porcine SIS), and (C) dermis and C.R. Bard XenMatrix (porcine dermis). Total nucleic acid concentration was assessed by UV absorbance at 260 nm. dsDNA concentration was assessed using PicoGreen dsDNA quantification reagent. Variability from isolation to isolation is depicted by SD. Data are means ± SD; n = 3 isolations per sample.

    Article Snippet: The proteinase K solution, Quant-iT PicoGreen dsDNA Assay Kit, and RNase A were obtained from Thermo Fisher Scientific.

    Techniques: Nucleic Acid Concentration, Concentration Assay, Isolation