proteinase k acrylic beads  (Millipore)


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    Structured Review

    Millipore proteinase k acrylic beads
    Effects of glial signals on the presence of autaptic activity in single RGCs Columns indicate the fraction of single RGCs that showed spontaneous, evoked or asynchronous EACs when cultured under different conditions. RGCs were grown in the absence of glial cells ( n = 47; RGC), in direct contact with glial cells from optic nerve ( n = 26; +OPN), in the presence of GCM ( n = 36; +GCM), in the presence of GCM that had been digested before with bead-coupled <t>proteinase</t> K ( n = 15; +GCM+ProtK) and in the presence of GCM and TTX ( n = 18; +GCM+TTX). Whole-cell EACs were recorded with the perforated-patch technique at a holding potential of −70 mV and analysed in a blinded fashion. Asterisks denote significant changes compared to glia-free conditions ( P
    Proteinase K Acrylic Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/proteinase k acrylic beads/product/Millipore
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    proteinase k acrylic beads - by Bioz Stars, 2020-03
    80/100 stars

    Images

    1) Product Images from "Glia-derived signals induce synapse formation in neurones of the rat central nervous system"

    Article Title: Glia-derived signals induce synapse formation in neurones of the rat central nervous system

    Journal: The Journal of Physiology

    doi: 10.1111/j.1469-7793.2001.00665.x

    Effects of glial signals on the presence of autaptic activity in single RGCs Columns indicate the fraction of single RGCs that showed spontaneous, evoked or asynchronous EACs when cultured under different conditions. RGCs were grown in the absence of glial cells ( n = 47; RGC), in direct contact with glial cells from optic nerve ( n = 26; +OPN), in the presence of GCM ( n = 36; +GCM), in the presence of GCM that had been digested before with bead-coupled proteinase K ( n = 15; +GCM+ProtK) and in the presence of GCM and TTX ( n = 18; +GCM+TTX). Whole-cell EACs were recorded with the perforated-patch technique at a holding potential of −70 mV and analysed in a blinded fashion. Asterisks denote significant changes compared to glia-free conditions ( P
    Figure Legend Snippet: Effects of glial signals on the presence of autaptic activity in single RGCs Columns indicate the fraction of single RGCs that showed spontaneous, evoked or asynchronous EACs when cultured under different conditions. RGCs were grown in the absence of glial cells ( n = 47; RGC), in direct contact with glial cells from optic nerve ( n = 26; +OPN), in the presence of GCM ( n = 36; +GCM), in the presence of GCM that had been digested before with bead-coupled proteinase K ( n = 15; +GCM+ProtK) and in the presence of GCM and TTX ( n = 18; +GCM+TTX). Whole-cell EACs were recorded with the perforated-patch technique at a holding potential of −70 mV and analysed in a blinded fashion. Asterisks denote significant changes compared to glia-free conditions ( P

    Techniques Used: Activity Assay, Cell Culture

    2) Product Images from "Novel Recombinant Engineered gp41 N-terminal Heptad Repeat Trimers and Their Potential as Anti-HIV-1 Therapeutics or Microbicides *"

    Article Title: Novel Recombinant Engineered gp41 N-terminal Heptad Repeat Trimers and Their Potential as Anti-HIV-1 Therapeutics or Microbicides *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M110.101170

    Proteinase K sensitivity of N28Fd. After digestion by proteinase K for different times, the residual amount of N28Fd or T20 was detected by a direct ELISA ( top panel ), and the remaining antiviral potency of N28Fd or T20 was tested by inhibition of HIV-1 IIIB assay ( bottom panel ).
    Figure Legend Snippet: Proteinase K sensitivity of N28Fd. After digestion by proteinase K for different times, the residual amount of N28Fd or T20 was detected by a direct ELISA ( top panel ), and the remaining antiviral potency of N28Fd or T20 was tested by inhibition of HIV-1 IIIB assay ( bottom panel ).

    Techniques Used: Direct ELISA, Inhibition

    Related Articles

    Recombinant:

    Article Title: RahU: An inducible and functionally pleiotropic protein in Pseudomonas aeruginosa modulates innate immunity and inflammation in host cells
    Article Snippet: Arachdoinc acid, Prednisone, Sodium deoxycholate, Proteinase K–Acrylic Beads, Tritirachium album and L-NMMA were purchased from Sigma-Aldrich. .. Recombinant human MCP-1/MCAF (CCL2) was purchased from PeproTech, (Rocky Hill, NJ).

    Ion Exchange Chromatography:

    Article Title: RahU: An inducible and functionally pleiotropic protein in Pseudomonas aeruginosa modulates innate immunity and inflammation in host cells
    Article Snippet: Lipopolysaccharide (LPS; L3024), which was extracted from Escherichia Coli 0111:B4 and purified by ion-exchange chromatography, was purchased from Sigma Aldrich (St. Louis, MO). .. Arachdoinc acid, Prednisone, Sodium deoxycholate, Proteinase K–Acrylic Beads, Tritirachium album and L-NMMA were purchased from Sigma-Aldrich.

    Incubation:

    Article Title: Novel Recombinant Engineered gp41 N-terminal Heptad Repeat Trimers and Their Potential as Anti-HIV-1 Therapeutics or Microbicides *
    Article Snippet: .. Peptide (40 μg/ml) was incubated at 37 °C in PBS containing 1 microunit/ml proteinase K-Acrylic Beads (Sigma). ..

    Article Title: Glia-derived signals induce synapse formation in neurones of the rat central nervous system
    Article Snippet: .. Briefly, GCM (2.2 ml) was incubated with proteinase K-acrylic beads (0.5 ml suspension, washed 10 times with PBS; Sigma) for 30 min at 37°C on a horizontal shaker. ..

    Purification:

    Article Title: RahU: An inducible and functionally pleiotropic protein in Pseudomonas aeruginosa modulates innate immunity and inflammation in host cells
    Article Snippet: Lipopolysaccharide (LPS; L3024), which was extracted from Escherichia Coli 0111:B4 and purified by ion-exchange chromatography, was purchased from Sigma Aldrich (St. Louis, MO). .. Arachdoinc acid, Prednisone, Sodium deoxycholate, Proteinase K–Acrylic Beads, Tritirachium album and L-NMMA were purchased from Sigma-Aldrich.

    Article Title: Glia-derived signals induce synapse formation in neurones of the rat central nervous system
    Article Snippet: Paragraph title: Microcultures of purified CNS neurones ... Briefly, GCM (2.2 ml) was incubated with proteinase K-acrylic beads (0.5 ml suspension, washed 10 times with PBS; Sigma) for 30 min at 37°C on a horizontal shaker.

    Protein Purification:

    Article Title: RahU: An inducible and functionally pleiotropic protein in Pseudomonas aeruginosa modulates innate immunity and inflammation in host cells
    Article Snippet: BD Talon His-tag protein purification kit was purchased from BD Bioscience (Bedford, MA). iScript One-step-RT-PCR kit, Criterion SD-PAGE and blotting systems, iQ5 Real-Time system, Coomassie Brilliant Blue R-250, Immuno-Star HRP Chemiluminescence kit, and Bradford reagent were acquired from Bio-Rad (Hercules, CA). .. Arachdoinc acid, Prednisone, Sodium deoxycholate, Proteinase K–Acrylic Beads, Tritirachium album and L-NMMA were purchased from Sigma-Aldrich.

    Generated:

    Article Title: Tobacco Upregulates P. gingivalis Fimbrial Proteins Which Induce TLR2 Hyposensitivity
    Article Snippet: Anti-P. gingivalis antibodies were generated in house, while HRP-linked anti-rabbit IgG came from Sigma-Aldrich (St. Louis, MO). .. Dextran, PBS and tetramethylbenzidine were obtained from Fisher Scientific (Fair Lawn, NJ), EDTA from AA Hoefer Inc. (San Francisco, CA), while trypan blue, insulin, transferrin, 2-mercaptoethanol, 2-aminoethanol, sodium selenite, proteinase K acrylic beads and phosphotongstic acid were purchased from Sigma-Aldrich (St. Louis, MO).

    Activity Assay:

    Article Title: Glia-derived signals induce synapse formation in neurones of the rat central nervous system
    Article Snippet: In some cultures, electrical activity was blocked chronically by adding tetrodotoxin (TTX) (10 μ m , Alomone Labs/ICS Clinical Service, Munich, Germany) to the culture medium. .. Briefly, GCM (2.2 ml) was incubated with proteinase K-acrylic beads (0.5 ml suspension, washed 10 times with PBS; Sigma) for 30 min at 37°C on a horizontal shaker.

    Binding Assay:

    Article Title: Novel Recombinant Engineered gp41 N-terminal Heptad Repeat Trimers and Their Potential as Anti-HIV-1 Therapeutics or Microbicides *
    Article Snippet: Peptide (40 μg/ml) was incubated at 37 °C in PBS containing 1 microunit/ml proteinase K-Acrylic Beads (Sigma). .. In brief, 50 μl of sample was coated onto wells of a 96-well polystyrene plate, followed by addition of rabbit antibodies directed against the HIV-1 gp41 N- and C-peptide mixture ( ) for binding N28Fd (at 1:400 dilution) and T20 (at 1:2000 dilution), respectively.

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    Millipore granzyme b tumor levels
    Response of triple-negative breast cancer (TNBC) patient-derived xenografts (PDXs) to the anti-programmed cell death protein 1 (anti-PD-1) therapy. a In vivo treatment with anti-PD-1 antibody (10 mg/kg intravenous [i.v.] once weekly) of either TNBC MC1 PDX-engrafted nonhumanized (left graph, n = 5) or humanized (right graph, n = 5) nonobese diabetic/severe combined immunodeficiency IL2R γ null (hNSG) mice. Tumor volume was measured twice weekly. b Kaplan-Meier analysis of median survival of mice treated with vehicle ( n = 6) vs. anti-PD-1 antibody ( n = 6). c hNSG mice engrafted with an additional TNBC BCM-4913 PDX tumor line were treated with either vehicle control or anti-PD-1 antibody (10 mg/kg i.v. once weekly). Tumor volumes were measured twice weekly. d In vivo treatment with anti-PD-1 antibody (10 mg/kg i.v. once weekly) of TNBC BCM-4664 ( n = 5) and HM-3818 ( n = 5) PDXs engrafted in hNSG mice. Tumor volume was measured twice weekly. e Analysis of tumor-infiltrating lymphocyte (TIL) cytotoxic activity. TILs isolated by Ficoll gradient from vehicle- or anti-PD-1 antibody-treated MC1 PDX tumors engrafted in hNSG mice were cocultured with disaggregated MC1 tumor cells obtained from the corresponding PDX grown in nonhumanized NSG mice. Cytotoxic activity was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay as per the manufacturer’s instructions. f Levels of <t>granzyme</t> B tumor were measured by incubating tumor protein lysates with antibody-immobilized magnetic beads and evaluated using a Luminex LX200 Multiplexing Assay System. ** P
    Granzyme B Tumor Levels, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/granzyme b tumor levels/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    granzyme b tumor levels - by Bioz Stars, 2020-03
    86/100 stars
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    Response of triple-negative breast cancer (TNBC) patient-derived xenografts (PDXs) to the anti-programmed cell death protein 1 (anti-PD-1) therapy. a In vivo treatment with anti-PD-1 antibody (10 mg/kg intravenous [i.v.] once weekly) of either TNBC MC1 PDX-engrafted nonhumanized (left graph, n = 5) or humanized (right graph, n = 5) nonobese diabetic/severe combined immunodeficiency IL2R γ null (hNSG) mice. Tumor volume was measured twice weekly. b Kaplan-Meier analysis of median survival of mice treated with vehicle ( n = 6) vs. anti-PD-1 antibody ( n = 6). c hNSG mice engrafted with an additional TNBC BCM-4913 PDX tumor line were treated with either vehicle control or anti-PD-1 antibody (10 mg/kg i.v. once weekly). Tumor volumes were measured twice weekly. d In vivo treatment with anti-PD-1 antibody (10 mg/kg i.v. once weekly) of TNBC BCM-4664 ( n = 5) and HM-3818 ( n = 5) PDXs engrafted in hNSG mice. Tumor volume was measured twice weekly. e Analysis of tumor-infiltrating lymphocyte (TIL) cytotoxic activity. TILs isolated by Ficoll gradient from vehicle- or anti-PD-1 antibody-treated MC1 PDX tumors engrafted in hNSG mice were cocultured with disaggregated MC1 tumor cells obtained from the corresponding PDX grown in nonhumanized NSG mice. Cytotoxic activity was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay as per the manufacturer’s instructions. f Levels of granzyme B tumor were measured by incubating tumor protein lysates with antibody-immobilized magnetic beads and evaluated using a Luminex LX200 Multiplexing Assay System. ** P

    Journal: Breast Cancer Research : BCR

    Article Title: Evaluation of anti-PD-1-based therapy against triple-negative breast cancer patient-derived xenograft tumors engrafted in humanized mouse models

    doi: 10.1186/s13058-018-1037-4

    Figure Lengend Snippet: Response of triple-negative breast cancer (TNBC) patient-derived xenografts (PDXs) to the anti-programmed cell death protein 1 (anti-PD-1) therapy. a In vivo treatment with anti-PD-1 antibody (10 mg/kg intravenous [i.v.] once weekly) of either TNBC MC1 PDX-engrafted nonhumanized (left graph, n = 5) or humanized (right graph, n = 5) nonobese diabetic/severe combined immunodeficiency IL2R γ null (hNSG) mice. Tumor volume was measured twice weekly. b Kaplan-Meier analysis of median survival of mice treated with vehicle ( n = 6) vs. anti-PD-1 antibody ( n = 6). c hNSG mice engrafted with an additional TNBC BCM-4913 PDX tumor line were treated with either vehicle control or anti-PD-1 antibody (10 mg/kg i.v. once weekly). Tumor volumes were measured twice weekly. d In vivo treatment with anti-PD-1 antibody (10 mg/kg i.v. once weekly) of TNBC BCM-4664 ( n = 5) and HM-3818 ( n = 5) PDXs engrafted in hNSG mice. Tumor volume was measured twice weekly. e Analysis of tumor-infiltrating lymphocyte (TIL) cytotoxic activity. TILs isolated by Ficoll gradient from vehicle- or anti-PD-1 antibody-treated MC1 PDX tumors engrafted in hNSG mice were cocultured with disaggregated MC1 tumor cells obtained from the corresponding PDX grown in nonhumanized NSG mice. Cytotoxic activity was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay as per the manufacturer’s instructions. f Levels of granzyme B tumor were measured by incubating tumor protein lysates with antibody-immobilized magnetic beads and evaluated using a Luminex LX200 Multiplexing Assay System. ** P

    Article Snippet: Granzyme B tumor levels were measured by incubating tumor protein lysates with antibody-immobilized magnetic beads (HGRNZMB-MAG; EMD Millipore, Billerica, MA) and evaluated using a Luminex LX-200 multiplexing assay system (Luminex Corp., Austin, TX, USA).

    Techniques: Derivative Assay, In Vivo, Mouse Assay, Activity Assay, Isolation, Cytotoxicity Assay, Magnetic Beads, Luminex, Multiplexing