Journal: Pharmaceutical Biology

Article Title: Emodin in Rheum undulatum inhibits oxidative stress in the liver via AMPK with Hippo/Yap signalling pathway

doi: 10.1080/13880209.2020.1750658

Figure Lengend Snippet: AMPKα and YAP co-relation mediated hepatocyte protective effect of emodin. (A) MTT assay of HeLa and HepG2 cells. HepG2 cells were incubated as describe in . (B) Reversed cell viability by compound C (a chemical inhibitor of AMPK) in HepG2 cells. HeLa cells or HepG2 cells were treated with 10 μM compound C for 30 min and incubated with 10 μM Emo for 1 h, continuously incubated with 10 μM AA for 12 h, and exposed to 5 μM iron for 2 h. All data represent means ± SD of three independent experiments ( **p < 0.01 between control and AA + iron treated cells; ##p < 0.01, #p < 0.05 between AA + iron treated cells with or without Emo). (C) Expression of p-LATS1, p-YAP and YAP in HepG2 cells. Hela cells or HepG2 cells were incubated in serum free media for 12 h and treated with 30 μM Emo for indicated time periods. (D) Compare of HeLa cells and HepG2 cells in expression of p-YAP. Com.C: compound C; Emo: emodin.

Article Snippet: Anti-PARP, anti-procaspase-3, anti-Bcl-xL, anti-phospho-acetyl-CoA carboxylase (ACC), anti-AMPKα, anti-phospho-AMPKα, anti-LKB1, anti-phospho-LKB1, anti-Yes-associated protein (YAP), and anti-phospho-YAP antibodies were obtained from Cell Signalling Technology (Beverly, MA, USA).

Techniques: MTT Assay, Incubation, Expressing