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Journal: Journal of Neuroinflammation
Article Title: Probiotics alleviate painful diabetic neuropathy by modulating the microbiota–gut–nerve axis in rats
doi: 10.1186/s12974-025-03352-3
Figure Lengend Snippet: Probiotics treatment mitigated intestinal barrier impairment and systemic inflammation in PDN rats. ( A ) Representative captures of H&E staining of the colon tissue. The red arrows indicate inflammatory cell infiltration. Scale bars = 100 μm. ( B ) Representative Western blot brands of ZO-1, occludin, and claudin-1 in the colon ( n = 6). ( C-E ) Quantification of ZO-1, occludin, and claudin-1 in the colon. β-actin was used as the loading control in Western blot analysis. ( F ) Representative captures of immunofluorescence of ZO-1 in the colon. Scale bars = 50 μm. ( G ) The intensity analysis of ZO-1 immunofluorescence staining. ( H-L ) Serum levels of LPS, LBP, IL-6, IL-1β, and TNF-α ( n = 10). Data are presented as mean ± SEM. Data are presented as mean ± SEM. *** P < 0.001 versus the control group; # P < 0.05, ## P < 0.01, ### P < 0.001 versus the PDN group, one-way ANOVA with Tukey’s multiple comparisons test for ( C-E ), ( G ) and ( H-L )
Article Snippet: Serum levels of interleukin-6 (IL-6), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) were measured using the ELISA kits (
Techniques: Probiotics, Staining, Western Blot, Control, Immunofluorescence