Journal: Nutrients
Article Title: Lactobacillus reuteri Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Modulating the Gut Microbiota in Mice
doi: 10.3390/nu15194256
Figure Lengend Snippet: L. reuteri reduces intestinal damage and repairs intestinal function. ( A ) Representative images of HE staining (magnification × 10, scale = 100 μm) and immunofluorescence staining for ZO-1, occludin, and MUC2 of colon tissues (magnification × 15, scale = 100 μm). ( B ) Serum LBP levels were measured by ELISA. The mRNA levels of ZO-1 ( C ), occludin ( D ), and MUC2 ( E ) (red indicate positive areas). Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001. NC, PBS + PBS group; LPS, PBS + LPS group; LR, L. reuteri + LPS group.
Article Snippet: The LPS binding protein (LBP) concentration was measured using an ELISA kit (ab269542, Abcam, Cambridge, UK), and a mouse Cytokine 23-Plex Assay (Kit Bio-Rad, Hercules, CA, USA) was used to measure the concentration of serum inflammatory factors.
Techniques: Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay