Journal: Cell Death & Disease
Article Title: Gut-derived lipopolysaccharide remodels tumoral microenvironment and synergizes with PD-L1 checkpoint blockade via TLR4/MyD88/AKT/NF-κB pathway in pancreatic cancer
doi: 10.1038/s41419-021-04293-4
Figure Lengend Snippet: A GEPIA analyzed TLR4 transcription data in PDAC tissues and normal tissues from the TCGA database. B western blot measured TLR4 protein expression in PDAC tumor tissues and adjacent normal tissues. C representative image of TLR4 expression in adjacent normal tissues and PDAC tissues. D LinkedOmics analyzed significantly enriched GO_BP annotations by GSEA of TLR4 in PDAC. E cBioportal tools analyzed the correlation between TLR4 and PD-L1 transcription in PDAC. F qRT-PCR measured TLR4 and PD-L1 mRNA expression in pancreatic cancer tissues. PDAC patient Serum LPS ( G ), serum LBP ( H ), and LPS/HDL ( I ) had a positive correlation with tumoral PD-L1 transcription. J representative image of TLR4 and PD-L1 co-immunofluorescence in PDAC tissues slice and CK19 staining in a continuous slice.
Article Snippet: Serum LPS-binding protein (LBP) concentration was detected by LBP enzyme-linked immunosorbent assay kit (Boster Biological Technology Co., Ltd), the procedures were followed the manual from the manufacturer.
Techniques: Western Blot, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining