Protein G Coated Agarose Bead Slurry, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Arming a Replicating Adenovirus with Osteoprotegerin Reduces the Tumor Burden in a Murine Model of Osteolytic Bone Metastases of Breast Cancer"
Article Title: Arming a Replicating Adenovirus with Osteoprotegerin Reduces the Tumor Burden in a Murine Model of Osteolytic Bone Metastases of Breast Cancer
Journal: Cancer gene therapy
Figure Legend Snippet: Characterization of armed CRAds. A, B, MDA-MB-231 cells were infected with Ad5-Δ24-sOPG-Fc, Ad5-Δ24-sOPG-Fc-RGD or Adwt300. At the indicated times, cellular RNA was subjected to QRT-PCR to detect expression of: A, the sOPG-Fc gene (cells infected with Ad5-Δ24-sOPG-Fc or Ad5-Δ24-sOPG-Fc-RGD) or the 14.7k gene (cells infected with Adwt300); and B, the ADP gene. The copy number was normalized to total cellular RNA. C, Secretion of sOPG-Fc. MDA-MB-231 cells were infected with Ad5-Δ24, Ad5-Δ24RGD, Ad5-Δ24-sOPG-Fc or Ad5-Δ24-sOPG-Fc-RGD. At the indicated times, medium was harvested, diluted 1:300 and expression of sOPG-Fc was detected in an ELISA. D , sOPG-Fc binds RANKL. Medium from MDA-MB-231 cells 48 h postinfection was incubated in the presence (+) or absence (−) of sRANKL and pulled down with protein G-agarose. Proteins were released and subjected to immunoblot using anti-RANKL primary antibody. C: sRANKL.
Techniques Used: Multiple Displacement Amplification, Infection, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Incubation