protein concentrators  (Thermo Fisher)


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    Structured Review

    Thermo Fisher protein concentrators
    Protein Concentrators, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentrators/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protein concentrators - by Bioz Stars, 2020-04
    94/100 stars

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    Related Articles

    Invasion Assay:

    Article Title: Transient mechanical strain promotes the maturation of invadopodia and enhances cancer cell invasion in vitro
    Article Snippet: The medium was then removed from the invasion assay cultures, and the cultures were rinsed twice with warmed 1× PBS. .. The collected medium was concentrated using protein concentrators with a molecular mass cut-off of 10 kDa (ThermoFisher).

    Article Title: The Role of PAK1 in the Maturation of Invadopodia During Transient Mechanical Stimulation
    Article Snippet: Conditioned Medium Collection To collect secreted MMP-2 enzyme, the HT1080 cells were allowed to attach as normal to the invasion assay as described above. .. The collected medium was concentrated using 10 kDa molecular weight cut off protein concentrators (Thermo Fisher Scientific).

    Cell Culture:

    Article Title: Mutual regulation between IGF-1R and IGFBP-3 in human corneal epithelial cells
    Article Snippet: .. Cell culture supernatants were concentrated using protein concentrators containing a polyether sulfone membrane (10K MWCO; Pierce, Rockford, IL). .. Cells were starved in basal media for 24 hours and then stimulated by 10 μg/ml of recombinant human insulin or 500 ng/ml of recombinant human IGFBP-3 for 24 hours.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Mutual regulation between IGF-1R and IGFBP-3 in human corneal epithelial cells
    Article Snippet: Paragraph title: Enzyme-linked Immunoassay (ELISA) ... Cell culture supernatants were concentrated using protein concentrators containing a polyether sulfone membrane (10K MWCO; Pierce, Rockford, IL).

    Protein Concentration:

    Article Title: Transient mechanical strain promotes the maturation of invadopodia and enhances cancer cell invasion in vitro
    Article Snippet: The collected medium was concentrated using protein concentrators with a molecular mass cut-off of 10 kDa (ThermoFisher). .. The collected medium was concentrated using protein concentrators with a molecular mass cut-off of 10 kDa (ThermoFisher).

    Article Title: The Role of PAK1 in the Maturation of Invadopodia During Transient Mechanical Stimulation
    Article Snippet: The collected medium was concentrated using 10 kDa molecular weight cut off protein concentrators (Thermo Fisher Scientific). .. The collected medium was concentrated using 10 kDa molecular weight cut off protein concentrators (Thermo Fisher Scientific).

    Article Title: Mutual regulation between IGF-1R and IGFBP-3 in human corneal epithelial cells
    Article Snippet: Cell culture supernatants were concentrated using protein concentrators containing a polyether sulfone membrane (10K MWCO; Pierce, Rockford, IL). .. Protein concentration was determined using a Qubit 3.0 Fluorometer (Thermo Fisher, Rockford, IL).

    Recombinant:

    Article Title: Mutual regulation between IGF-1R and IGFBP-3 in human corneal epithelial cells
    Article Snippet: Cell culture supernatants were concentrated using protein concentrators containing a polyether sulfone membrane (10K MWCO; Pierce, Rockford, IL). .. Cells were starved in basal media for 24 hours and then stimulated by 10 μg/ml of recombinant human insulin or 500 ng/ml of recombinant human IGFBP-3 for 24 hours.

    Molecular Weight:

    Article Title: The Role of PAK1 in the Maturation of Invadopodia During Transient Mechanical Stimulation
    Article Snippet: .. The collected medium was concentrated using 10 kDa molecular weight cut off protein concentrators (Thermo Fisher Scientific). .. Protein concentration was determined by the DC protein assay (Bio-Rad).

    DC Protein Assay:

    Article Title: Transient mechanical strain promotes the maturation of invadopodia and enhances cancer cell invasion in vitro
    Article Snippet: The collected medium was concentrated using protein concentrators with a molecular mass cut-off of 10 kDa (ThermoFisher). .. The collected medium was concentrated using protein concentrators with a molecular mass cut-off of 10 kDa (ThermoFisher).

    Article Title: The Role of PAK1 in the Maturation of Invadopodia During Transient Mechanical Stimulation
    Article Snippet: The collected medium was concentrated using 10 kDa molecular weight cut off protein concentrators (Thermo Fisher Scientific). .. The collected medium was concentrated using 10 kDa molecular weight cut off protein concentrators (Thermo Fisher Scientific).

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  • 80
    Thermo Fisher gastrocnemius protein analysis intramuscular concentrations
    <t>Intramuscular</t> <t>protein</t> <t>concentrations.</t> Intramuscular concentrations of myostatin, IGF-1 and TNF-α were measured in homogenized <t>gastrocnemius</t> muscles. Exercise was found to have significantly lowered intramuscular myostatin concentrations (p
    Gastrocnemius Protein Analysis Intramuscular Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gastrocnemius protein analysis intramuscular concentrations/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gastrocnemius protein analysis intramuscular concentrations - by Bioz Stars, 2020-04
    80/100 stars
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    94
    Thermo Fisher single molecule array simoa technology protein concentrations
    Plasma <t>NDE</t> levels of Aβ40, total tau, and NFL are not significantly different between individuals with and without mTBI. Plasma NDE levels of Aβ40 (A) , NFL (B) , and total tau (C) , were not significantly different between the two populations while plasma ADE Aβ40 (A) , NFL (B) , and total tau (C) were not detectable by the <t>Simoa</t> Assay.
    Single Molecule Array Simoa Technology Protein Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/single molecule array simoa technology protein concentrations/product/Thermo Fisher
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    single molecule array simoa technology protein concentrations - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    95
    Thermo Fisher 150 kd protein concentrator
    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of <t>50–150</t> nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.
    150 Kd Protein Concentrator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/150 kd protein concentrator/product/Thermo Fisher
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    150 kd protein concentrator - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    80
    Thermo Fisher protein concentration determination protein concentrations
    Acer3 deficiency inhibits apoptosis and oxidative stress in mouse hepatocytes in response to overload of palmitate. a and b Mouse primary hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 24 h were stained with ORO a and the optical density of ORO staining was measured as described in “Materials and methods” section b . c Hepatocytes were treated with BSA or BSA–palmitate complex at indicated <t>concentrations</t> for 24 h before cell viability was determined by MTT assays as described in “Materials and methods” section. d hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for indicated time durations were subjected to Western blot analyses with an antibody against cleaved capase3 or β-Actin (a <t>protein-loading</t> control). e Hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 12 h were subjected to Western blot analyses with the antibody against 4-HNE or β-Actin (a protein-loading control). f and g DHE staining was performed to measure the production of reactive oxidation species in hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 12 h. Hepatocytes stained with DHE were imaged by microscopy g with background in white and the staining in black, and the florescent intensity was quantified as described in “Materials and methods” section h . Data in b , c , and g represent mean ± SD of three independent experiments. Images in a , d , e , and f represent results from three independent experiments. * P
    Protein Concentration Determination Protein Concentrations, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protein concentration determination protein concentrations/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protein concentration determination protein concentrations - by Bioz Stars, 2020-04
    80/100 stars
      Buy from Supplier

    Image Search Results


    Intramuscular protein concentrations. Intramuscular concentrations of myostatin, IGF-1 and TNF-α were measured in homogenized gastrocnemius muscles. Exercise was found to have significantly lowered intramuscular myostatin concentrations (p

    Journal: European Journal of Translational Myology

    Article Title: Exercise preserves muscle mass and force in a prostate cancer mouse model

    doi: 10.4081/ejtm.2019.8520

    Figure Lengend Snippet: Intramuscular protein concentrations. Intramuscular concentrations of myostatin, IGF-1 and TNF-α were measured in homogenized gastrocnemius muscles. Exercise was found to have significantly lowered intramuscular myostatin concentrations (p

    Article Snippet: Gastrocnemius Protein Analysis Intramuscular concentrations of IGF-1 (ThermoScientific, Frederick, MD), myostatin (R & D Systems, Minneapolis, MN), and TNF-α (ThermoFisher Scientific, Carlsbad, CA) were quantified in homogenized gastrocnemius muscle using commercially available assay kits following the manufacturer’s recommended protocol.

    Techniques:

    Plasma NDE levels of Aβ40, total tau, and NFL are not significantly different between individuals with and without mTBI. Plasma NDE levels of Aβ40 (A) , NFL (B) , and total tau (C) , were not significantly different between the two populations while plasma ADE Aβ40 (A) , NFL (B) , and total tau (C) were not detectable by the Simoa Assay.

    Journal: Frontiers in Neuroscience

    Article Title: Assessing Neuronal and Astrocyte Derived Exosomes From Individuals With Mild Traumatic Brain Injury for Markers of Neurodegeneration and Cytotoxic Activity

    doi: 10.3389/fnins.2019.01005

    Figure Lengend Snippet: Plasma NDE levels of Aβ40, total tau, and NFL are not significantly different between individuals with and without mTBI. Plasma NDE levels of Aβ40 (A) , NFL (B) , and total tau (C) , were not significantly different between the two populations while plasma ADE Aβ40 (A) , NFL (B) , and total tau (C) were not detectable by the Simoa Assay.

    Article Snippet: Quantification of NDE and ADE Protein Cargo by Human-Specific Enzyme Linked Immunosorbent Assays (ELISAs) and Using the Single Molecule Array (Simoa) Technology Protein concentrations for eluted NDE and ADE suspensions were determined using the Pierce bicinchoninic acid (BCA) Protein Assay kit (Thermo Fisher Scientific; Catalog # 23225).

    Techniques:

    SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Journal: Cancers

    Article Title: The Transfer of Sphingomyelinase Contributes to Drug Resistance in Multiple Myeloma

    doi: 10.3390/cancers11121823

    Figure Lengend Snippet: SMPD1 expression levels correlate with total SMase content in supernatant in MM cell lines and their exosomes. ( A ) Basal-dCT values of all four genes coding for SMases (SMPD1–4) measured by qRT-PCR in four human MM cell lines (JJN3, OPM2, LP1, and U266) after 24 h of culture. The housekeeping gene ABL1 was used for data normalization, and differential gene expression was determined using the comparative ΔΔCt method. ( B ) Basal total SMase content determined in supernatans by an AmplexRed SMase assay after 24 h of culture for all four cell lines. The amount of SMase activity measured in these cell lines corresponded best to SMPD1 expression, coding for acid SMase (ASM). ( C ) Cells and exosomes were isolated after 24 h of culture and analyzed for ASM content and exosomal markers, CD81 and CD63. Nanoparticle tracking analysis by Zetaview analysis shows a mean diameter of 50–150 nm, confirming the expected size of exosomes. Immunoblot can be found in Supplementary Figure S4B . ASM = acid sphingomyelinase, exo = exosomes. In A and B, the averages of n > 3 are shown.

    Article Snippet: The filtered medium was concentrated using a 150 kD Protein Concentrator (Thermo Scientific, Waltham, MA, USA) and filtered again with a 0.22 µM pore filter.

    Techniques: Expressing, Quantitative RT-PCR, Activity Assay, Isolation

    Acer3 deficiency inhibits apoptosis and oxidative stress in mouse hepatocytes in response to overload of palmitate. a and b Mouse primary hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 24 h were stained with ORO a and the optical density of ORO staining was measured as described in “Materials and methods” section b . c Hepatocytes were treated with BSA or BSA–palmitate complex at indicated concentrations for 24 h before cell viability was determined by MTT assays as described in “Materials and methods” section. d hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for indicated time durations were subjected to Western blot analyses with an antibody against cleaved capase3 or β-Actin (a protein-loading control). e Hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 12 h were subjected to Western blot analyses with the antibody against 4-HNE or β-Actin (a protein-loading control). f and g DHE staining was performed to measure the production of reactive oxidation species in hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 12 h. Hepatocytes stained with DHE were imaged by microscopy g with background in white and the staining in black, and the florescent intensity was quantified as described in “Materials and methods” section h . Data in b , c , and g represent mean ± SD of three independent experiments. Images in a , d , e , and f represent results from three independent experiments. * P

    Journal: Cell Death & Disease

    Article Title: Targeting alkaline ceramidase 3 alleviates the severity of nonalcoholic steatohepatitis by reducing oxidative stress

    doi: 10.1038/s41419-019-2214-9

    Figure Lengend Snippet: Acer3 deficiency inhibits apoptosis and oxidative stress in mouse hepatocytes in response to overload of palmitate. a and b Mouse primary hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 24 h were stained with ORO a and the optical density of ORO staining was measured as described in “Materials and methods” section b . c Hepatocytes were treated with BSA or BSA–palmitate complex at indicated concentrations for 24 h before cell viability was determined by MTT assays as described in “Materials and methods” section. d hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for indicated time durations were subjected to Western blot analyses with an antibody against cleaved capase3 or β-Actin (a protein-loading control). e Hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 12 h were subjected to Western blot analyses with the antibody against 4-HNE or β-Actin (a protein-loading control). f and g DHE staining was performed to measure the production of reactive oxidation species in hepatocytes treated with BSA or BSA–palmitate complex (500 μM) for 12 h. Hepatocytes stained with DHE were imaged by microscopy g with background in white and the staining in black, and the florescent intensity was quantified as described in “Materials and methods” section h . Data in b , c , and g represent mean ± SD of three independent experiments. Images in a , d , e , and f represent results from three independent experiments. * P

    Article Snippet: Protein concentration determination Protein concentrations were determined with BSA as a standard using a bicinchoninic acid (BCA) protein determination kit (Thermo Scientific; Waltham, MA, USA) according to the manufacturer’s instructions.

    Techniques: Staining, MTT Assay, Western Blot, Microscopy