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Bio-Rad protein concentrations
Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Clone Assay:

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: Full-length OMgp, and Na+ channel β1 and β2 extracellular portions were cloned into the pGEX-KG vector for expression in E. coli and purified as GST-fusion proteins following standard procedures ( ). .. Protein concentrations were determined using the BCA protein assay kit (BioRad).

Centrifugation:

Article Title: Protein Import into Hydrogenosomes of Trichomonas vaginalis Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ †
Article Snippet: The cytosolic fraction (supernatant) was obtained by subsequent centrifugation of the whole-cell lysate at 8,000 × g for 15 min at 4°C. .. Protein concentrations were determined with the Bradford assay kit (Bio-Rad) according to the manufacturer's instructions.

Filtration:

Article Title: Methionine Sulfoxide Reductase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis, an Enzyme Designed To Function at Suboptimal Growth Temperatures ▿
Article Snippet: Finally, all three protein solutions were subjected to gel filtration with a Superdex 200 HR 10/30 column (Amersham Biosciences), with a mobile phase of 50 mM sodium phosphate buffer (pH 7.0) containing 100 mM NaCl and 1 mM DTT and a flow rate of 0.3 ml min−1 . .. Protein concentrations were determined with a protein assay kit (Bio-Rad) with bovine serum albumin as the standard.

Article Title: In vitro reconstitution of the human RISC-loading complex
Article Snippet: The unbound material was collected, concentrated to 1 ml, and applied to a Superdex 200 16/60 column (Amersham Pharmacia) equilibrated in gel filtration buffer [100 mM KCl, 5% glycerol, 1 mM DTT, and 20 mM Hepes (pH 7.5)]. .. Protein concentrations were determined by the Bradford assay (Bio-Rad).

Polyacrylamide Gel Electrophoresis:

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: .. Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques. .. Primary antibodies were obtained from the following sources: α -phosphorylated pT202/pY204 ERK1/2 and total ERK1/2 (Cell Signaling Technology, Davis, MA, USA), α -caspase 3, α -BIM (Calbiochem), α -BCL-2 (Santa Cruz Antibodies, Santa Cruz, CA, USA), α -cytochrome C, α -cytochrome C Oxidase IV (Invitrogen, Carlsbad, CA, USA), α -Smac/DIABLO (BD Pharmingen), α -MCL-1 (Invitrogen) and α - β -tubulin (Sigma).

Bradford Assay:

Article Title: Regiospecific Chlorination of (S)-?-Tyrosyl-S-Carrier Protein Catalyzed by SgcC3 in the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027
Article Snippet: .. Protein concentrations were determined using the Bradford assay (Bio-Rad, Hercules, CA) .. SgcC3 was denatured by heat or with 50% methanol (final concentration) to release any noncovlently-bound cofactor.

Article Title: p27kip1 Deficiency Impairs G2/M Arrest in Response to DNA Damage, Leading to an Increase in Genetic Instability ▿
Article Snippet: .. Protein concentrations were measured using the Bradford assay (Bio-Rad). .. Cyclin B1 agarose-conjugated antibody (Santa Cruz, sc-245AC) (5 μg) was incubated with lysates (500 μg) plus BSA at 0.5 mg/ml for 12 h at 4°C.

Article Title: Protein Import into Hydrogenosomes of Trichomonas vaginalis Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ †
Article Snippet: .. Protein concentrations were determined with the Bradford assay kit (Bio-Rad) according to the manufacturer's instructions. .. The activity of malic enzyme was assayed by monitoring the reduction of NADP+ as described previously ( ).

Article Title: In vitro reconstitution of the human RISC-loading complex
Article Snippet: .. Protein concentrations were determined by the Bradford assay (Bio-Rad). .. First, 705 μg (3 nmol) of Dicer, 600 μg (6 nmol) of Ago2, and 550 μg (11 nmol, assuming a dimer) of TRBP were mixed in 250 μl (final volume) of gel filtration buffer.

Electrophoresis:

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: .. Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques. .. Primary antibodies were obtained from the following sources: α -phosphorylated pT202/pY204 ERK1/2 and total ERK1/2 (Cell Signaling Technology, Davis, MA, USA), α -caspase 3, α -BIM (Calbiochem), α -BCL-2 (Santa Cruz Antibodies, Santa Cruz, CA, USA), α -cytochrome C, α -cytochrome C Oxidase IV (Invitrogen, Carlsbad, CA, USA), α -Smac/DIABLO (BD Pharmingen), α -MCL-1 (Invitrogen) and α - β -tubulin (Sigma).

Incubation:

Article Title: Human Mitochondrial Ribosomal Protein MRPL12 Interacts Directly with Mitochondrial RNA Polymerase to Modulate Mitochondrial Gene Expression *
Article Snippet: Thrombin-agarose (Sigma) was added to the eluted GST-MRPL12 and incubated for 4 h at room temperature with rotation. .. Protein concentrations were determined with a Bio-Rad protein assay kit using bovine serum albumin as a standard.

Article Title: p27kip1 Deficiency Impairs G2/M Arrest in Response to DNA Damage, Leading to an Increase in Genetic Instability ▿
Article Snippet: Protein concentrations were measured using the Bradford assay (Bio-Rad). .. Cyclin B1 agarose-conjugated antibody (Santa Cruz, sc-245AC) (5 μg) was incubated with lysates (500 μg) plus BSA at 0.5 mg/ml for 12 h at 4°C.

Cell Culture:

Article Title: Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity
Article Snippet: Subsequently, adult O. viverrini were cultured to collect eggs and excretory-secretory (ES) products as described ( ). .. Protein concentrations of O. viverrini extracts were determined by the Bradford protein assay (Bio-Rad, Hercules, CA, USA) after which parasite extracts were stored at −80°C until required.

Expressing:

Article Title: Human Mitochondrial Ribosomal Protein MRPL12 Interacts Directly with Mitochondrial RNA Polymerase to Modulate Mitochondrial Gene Expression *
Article Snippet: Paragraph title: Expression and Purification of Recombinant MRPL12 ... Protein concentrations were determined with a Bio-Rad protein assay kit using bovine serum albumin as a standard.

Article Title: Methionine Sulfoxide Reductase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis, an Enzyme Designed To Function at Suboptimal Growth Temperatures ▿
Article Snippet: Paragraph title: Expression and purification of MsrA, MsrB, and MsrAB. ... Protein concentrations were determined with a protein assay kit (Bio-Rad) with bovine serum albumin as the standard.

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: Full-length OMgp, and Na+ channel β1 and β2 extracellular portions were cloned into the pGEX-KG vector for expression in E. coli and purified as GST-fusion proteins following standard procedures ( ). .. Protein concentrations were determined using the BCA protein assay kit (BioRad).

Cell Fractionation:

Article Title: Protein Import into Hydrogenosomes of Trichomonas vaginalis Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ †
Article Snippet: Paragraph title: Cell fractionation and protein concentration determination. ... Protein concentrations were determined with the Bradford assay kit (Bio-Rad) according to the manufacturer's instructions.

Western Blot:

Article Title: Macrophage EP4 deficiency increases apoptosis and suppresses early atherosclerosis
Article Snippet: Paragraph title: Western blotting ... Protein concentrations were determined with the DC Protein assay kit (Bio-Rad Laboratories).

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: .. Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques. .. Primary antibodies were obtained from the following sources: α -phosphorylated pT202/pY204 ERK1/2 and total ERK1/2 (Cell Signaling Technology, Davis, MA, USA), α -caspase 3, α -BIM (Calbiochem), α -BCL-2 (Santa Cruz Antibodies, Santa Cruz, CA, USA), α -cytochrome C, α -cytochrome C Oxidase IV (Invitrogen, Carlsbad, CA, USA), α -Smac/DIABLO (BD Pharmingen), α -MCL-1 (Invitrogen) and α - β -tubulin (Sigma).

Kinase Assay:

Article Title: p27kip1 Deficiency Impairs G2/M Arrest in Response to DNA Damage, Leading to an Increase in Genetic Instability ▿
Article Snippet: Paragraph title: Cyclin B1-associated kinase assay. ... Protein concentrations were measured using the Bradford assay (Bio-Rad).

Flow Cytometry:

Article Title: Methionine Sulfoxide Reductase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis, an Enzyme Designed To Function at Suboptimal Growth Temperatures ▿
Article Snippet: Finally, all three protein solutions were subjected to gel filtration with a Superdex 200 HR 10/30 column (Amersham Biosciences), with a mobile phase of 50 mM sodium phosphate buffer (pH 7.0) containing 100 mM NaCl and 1 mM DTT and a flow rate of 0.3 ml min−1 . .. Protein concentrations were determined with a protein assay kit (Bio-Rad) with bovine serum albumin as the standard.

Immunoprecipitation:

Article Title: p27kip1 Deficiency Impairs G2/M Arrest in Response to DNA Damage, Leading to an Increase in Genetic Instability ▿
Article Snippet: Protein concentrations were measured using the Bradford assay (Bio-Rad). .. Immunoprecipitated protein complexes [cyclinB1/Cdk1(Cdc2)] were washed twice in lysis buffer and twice in kinase reaction buffer (80 mM β-glycerophosphate [pH 7.3], 20 mM EGTA, 15 mM MgCl2, 10 mM dithiothreitol).

Protease Inhibitor:

Article Title: Inflammatory Signaling and Aryl Hydrocarbon Receptor Mediate Synergistic Induction of Interleukin 6 in MCF-7 Cells
Article Snippet: Whole-cell extracts were prepared by lysing cells in 1• radioimmunoprecipitation assay buffer [RIPA; 10 mmol/L Tris-HCl (pH 8.0), 1 mmol/L EDTA, 0.5 mmol/L EGTA, 140 mmol/L NaCl, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS] buffer supplemented with 1% NP40, 300 mmol/L NaCl, and protease inhibitor cocktail (Sigma). .. Protein concentrations were determined using the detergent compatible Bio-Rad DC protein assay kit (Bio-Rad).

Infection:

Article Title: Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity
Article Snippet: The hamsters were euthanized 2–3 months after infection, necropsied, and adult O. viverrini flukes recovered from the bile ducts. .. Protein concentrations of O. viverrini extracts were determined by the Bradford protein assay (Bio-Rad, Hercules, CA, USA) after which parasite extracts were stored at −80°C until required.

Generated:

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques. .. The phospho-serine 69 specific anti-BIM-EL monoclonal antibody was generated in collaboration with BD BioSciences and details of its characterization will be published elsewhere (Cagnol, S. and McMahon, M., unpublished data).

other:

Article Title: Induction of cytochrome P450 1A by cow milk-based formula: a comparative study between human milk and formula
Article Snippet: Protein concentrations were measured with the Bio-Rad Protein Assay kit according to the manufacturer's instructions (Bio-Rad, Hercules, CA, U.S.A.).

Protein Concentration:

Article Title: Protein Import into Hydrogenosomes of Trichomonas vaginalis Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ †
Article Snippet: Paragraph title: Cell fractionation and protein concentration determination. ... Protein concentrations were determined with the Bradford assay kit (Bio-Rad) according to the manufacturer's instructions.

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: .. Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques. .. Primary antibodies were obtained from the following sources: α -phosphorylated pT202/pY204 ERK1/2 and total ERK1/2 (Cell Signaling Technology, Davis, MA, USA), α -caspase 3, α -BIM (Calbiochem), α -BCL-2 (Santa Cruz Antibodies, Santa Cruz, CA, USA), α -cytochrome C, α -cytochrome C Oxidase IV (Invitrogen, Carlsbad, CA, USA), α -Smac/DIABLO (BD Pharmingen), α -MCL-1 (Invitrogen) and α - β -tubulin (Sigma).

Recombinant:

Article Title: Human Mitochondrial Ribosomal Protein MRPL12 Interacts Directly with Mitochondrial RNA Polymerase to Modulate Mitochondrial Gene Expression *
Article Snippet: Paragraph title: Expression and Purification of Recombinant MRPL12 ... Protein concentrations were determined with a Bio-Rad protein assay kit using bovine serum albumin as a standard.

Article Title: Methionine Sulfoxide Reductase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis, an Enzyme Designed To Function at Suboptimal Growth Temperatures ▿
Article Snippet: Recombinant proteins were eluted with a linear gradient of 0 to 1 M NaCl in buffer A. .. Protein concentrations were determined with a protein assay kit (Bio-Rad) with bovine serum albumin as the standard.

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: Paragraph title: Production of recombinant proteins ... Protein concentrations were determined using the BCA protein assay kit (BioRad).

DC Protein Assay:

Article Title: Macrophage EP4 deficiency increases apoptosis and suppresses early atherosclerosis
Article Snippet: .. Protein concentrations were determined with the DC Protein assay kit (Bio-Rad Laboratories). .. Lysates (20 or 100 μg/ lane ) were resolved by NuPAGE Bis-Tris elecrophoresis (Invitrogen) and transferred onto polyvinylidene difluoride nitrocellulose membranes (Amersham Bioscience).

Article Title: Inflammatory Signaling and Aryl Hydrocarbon Receptor Mediate Synergistic Induction of Interleukin 6 in MCF-7 Cells
Article Snippet: .. Protein concentrations were determined using the detergent compatible Bio-Rad DC protein assay kit (Bio-Rad). .. Protein samples were resolved by Tricine SDS-PAGE and transferred to membrane.

Radioactivity:

Article Title: Transport Mechanisms of Carnosine in SKPT Cells: Contribution of Apical and Basolateral Membrane Transporters
Article Snippet: Radioactivity was measured in solubilized cells (and buffer) with a dual-channel liquid scintillation counter (Beckman LS 6000 SC; Beckman Coulter Inc., Fullerton, CA). .. Protein concentrations were measured using the Bio-Rad DC protein assay (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin as the standard.

Isolation:

Article Title: Protein Import into Hydrogenosomes of Trichomonas vaginalis Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ Involves both N-Terminal and Internal Targeting Signals: a Case Study of Thioredoxin Reductases ▿ †
Article Snippet: Isolation of hydrogenosomes was based on the method described by Bradley et al. ( ) with modifications ( ). .. Protein concentrations were determined with the Bradford assay kit (Bio-Rad) according to the manufacturer's instructions.

Microscopy:

Article Title: Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity
Article Snippet: After several washes with normal saline, metacercariae were collected, identified under a dissecting microscope, and viable metacercariae were used to infect hamsters ( Mesocricetus auratas ) by stomach intubation. .. Protein concentrations of O. viverrini extracts were determined by the Bradford protein assay (Bio-Rad, Hercules, CA, USA) after which parasite extracts were stored at −80°C until required.

Purification:

Article Title: Regiospecific Chlorination of (S)-?-Tyrosyl-S-Carrier Protein Catalyzed by SgcC3 in the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027
Article Snippet: Paragraph title: Overproduction and Purification of SgcC1, SgcC2, SgcC3, SgcE6 and Fre ... Protein concentrations were determined using the Bradford assay (Bio-Rad, Hercules, CA)

Article Title: Human Mitochondrial Ribosomal Protein MRPL12 Interacts Directly with Mitochondrial RNA Polymerase to Modulate Mitochondrial Gene Expression *
Article Snippet: Paragraph title: Expression and Purification of Recombinant MRPL12 ... Protein concentrations were determined with a Bio-Rad protein assay kit using bovine serum albumin as a standard.

Article Title: Methionine Sulfoxide Reductase from the Hyperthermophilic Archaeon Thermococcus kodakaraensis, an Enzyme Designed To Function at Suboptimal Growth Temperatures ▿
Article Snippet: Paragraph title: Expression and purification of MsrA, MsrB, and MsrAB. ... Protein concentrations were determined with a protein assay kit (Bio-Rad) with bovine serum albumin as the standard.

Article Title: In vitro reconstitution of the human RISC-loading complex
Article Snippet: Paragraph title: Purification of Dicer, Ago2, and TRBP. ... Protein concentrations were determined by the Bradford assay (Bio-Rad).

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: Full-length OMgp, and Na+ channel β1 and β2 extracellular portions were cloned into the pGEX-KG vector for expression in E. coli and purified as GST-fusion proteins following standard procedures ( ). .. Protein concentrations were determined using the BCA protein assay kit (BioRad).

Bradford Protein Assay:

Article Title: Ov-APR-1, an aspartic protease from the carcinogenic liver fluke, Opisthorchis viverrini: functional expression, immunolocalization and subsite specificity
Article Snippet: .. Protein concentrations of O. viverrini extracts were determined by the Bradford protein assay (Bio-Rad, Hercules, CA, USA) after which parasite extracts were stored at −80°C until required. .. Total RNA from each stage of O. viverrini was extracted with TRIzol (Invitrogen) according to the manufacturer’s instructions.

Lysis:

Article Title: Macrophage EP4 deficiency increases apoptosis and suppresses early atherosclerosis
Article Snippet: Cells were lysed on ice with a lysis buffer (Cell Signaling Technology, Danvers, MA) containing a protease (Sigma) and phosphatase (Pierce) inhibitor cocktails. .. Protein concentrations were determined with the DC Protein assay kit (Bio-Rad Laboratories).

Article Title: Human Mitochondrial Ribosomal Protein MRPL12 Interacts Directly with Mitochondrial RNA Polymerase to Modulate Mitochondrial Gene Expression *
Article Snippet: The MRPL12-bound beads were then loaded onto a column and washed five times with one bed volume of lysis buffer. .. Protein concentrations were determined with a Bio-Rad protein assay kit using bovine serum albumin as a standard.

Article Title: p27kip1 Deficiency Impairs G2/M Arrest in Response to DNA Damage, Leading to an Increase in Genetic Instability ▿
Article Snippet: Protein concentrations were measured using the Bradford assay (Bio-Rad). .. Immunoprecipitated protein complexes [cyclinB1/Cdk1(Cdc2)] were washed twice in lysis buffer and twice in kinase reaction buffer (80 mM β-glycerophosphate [pH 7.3], 20 mM EGTA, 15 mM MgCl2, 10 mM dithiothreitol).

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: Whole cell lysates were prepared using radio-immunoprecipitation assay (RIPA) lysis buffer as described previously ( ). .. Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques.

SDS Page:

Article Title: Inflammatory Signaling and Aryl Hydrocarbon Receptor Mediate Synergistic Induction of Interleukin 6 in MCF-7 Cells
Article Snippet: Protein concentrations were determined using the detergent compatible Bio-Rad DC protein assay kit (Bio-Rad). .. Protein samples were resolved by Tricine SDS-PAGE and transferred to membrane.

Plasmid Preparation:

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: Full-length OMgp, and Na+ channel β1 and β2 extracellular portions were cloned into the pGEX-KG vector for expression in E. coli and purified as GST-fusion proteins following standard procedures ( ). .. Protein concentrations were determined using the BCA protein assay kit (BioRad).

Binding Assay:

Article Title: Transport Mechanisms of Carnosine in SKPT Cells: Contribution of Apical and Basolateral Membrane Transporters
Article Snippet: Protein concentrations were measured using the Bio-Rad DC protein assay (Bio-Rad Laboratories, Hercules, CA) with bovine serum albumin as the standard. .. Mannitol was used to correct the uptake data of carnosine due to filter binding and extracellular content ( , ), as well as the transepithelial transport of carnosine due to paracellular flux ( ).

Article Title: Inflammatory Signaling and Aryl Hydrocarbon Receptor Mediate Synergistic Induction of Interleukin 6 in MCF-7 Cells
Article Snippet: Protein concentrations were determined using the detergent compatible Bio-Rad DC protein assay kit (Bio-Rad). .. Immunoblotting was performed using antibodies directed against AHR (Biomol), CYP1A1, ARNT, c-JUN, CAAT/enhancer binding protein h (C/EBPh), RELA (Santa Cruz), and p23 (provided by Dr. David Toft, Mayo Clinic).

In Vitro:

Article Title: Human Mitochondrial Ribosomal Protein MRPL12 Interacts Directly with Mitochondrial RNA Polymerase to Modulate Mitochondrial Gene Expression *
Article Snippet: Fractions from this column containing recombinant untagged MRPL12 were used for all in vitro assays. .. Protein concentrations were determined with a Bio-Rad protein assay kit using bovine serum albumin as a standard.

Radio Immunoprecipitation:

Article Title: Oncogenic BRAFV600E inhibits BIM expression to promote melanoma cell survival
Article Snippet: Whole cell lysates were prepared using radio-immunoprecipitation assay (RIPA) lysis buffer as described previously ( ). .. Protein concentrations were estimated using Coomassie protein concentration reagent (BioRad, Hercules, CA, USA) according to the manufacturer’s instructions, and equal amounts of protein were analyzed by SDS-polyacrylamide gels electrophoresis (PAGE), followed by transfer to polyvinylidene difluoride membranes (PVDF; Millipore, Billeria, MA, USA), and Western blot analysis using standard techniques.

Article Title: Inflammatory Signaling and Aryl Hydrocarbon Receptor Mediate Synergistic Induction of Interleukin 6 in MCF-7 Cells
Article Snippet: Whole-cell extracts were prepared by lysing cells in 1• radioimmunoprecipitation assay buffer [RIPA; 10 mmol/L Tris-HCl (pH 8.0), 1 mmol/L EDTA, 0.5 mmol/L EGTA, 140 mmol/L NaCl, 1% Triton X-100, 0.1% Na-deoxycholate, 0.1% SDS] buffer supplemented with 1% NP40, 300 mmol/L NaCl, and protease inhibitor cocktail (Sigma). .. Protein concentrations were determined using the detergent compatible Bio-Rad DC protein assay kit (Bio-Rad).

Concentration Assay:

Article Title: Regiospecific Chlorination of (S)-?-Tyrosyl-S-Carrier Protein Catalyzed by SgcC3 in the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027
Article Snippet: After concentration with an Amicon Ultra-4 (5K or 10K), the purified proteins were stored at −25 °C in 40% glycerol. .. Protein concentrations were determined using the Bradford assay (Bio-Rad, Hercules, CA)

Staining:

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: Recombinant proteins and GST were visualized by Coomassie brilliant blue staining. .. Protein concentrations were determined using the BCA protein assay kit (BioRad).

BIA-KA:

Article Title: Oligodendrocytes regulate formation of nodes of Ranvier via the recognition molecule OMgp
Article Snippet: .. Protein concentrations were determined using the BCA protein assay kit (BioRad). ..

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    Bio-Rad γ secretase
    Phenylalanine blocking mutations at both ε cleavage sites reduces APP cleavage but not binding to <t>γ-secretase.</t> ( A ) Western blot of γ-secretase cleavage of WT, V50F, M51F and V50F-M51F C100-FLAG. Duplicates from each substrate represent separate independent data points. * denotes a degradation product which co-purified with the substrate. ( B ) Cleavage of WT and V50F-M51F C100-FLAG over time. ( C ) Co-immunoprecipitation of Myc-tagged WT or V50F-M51F C100 substrate. Duplicates are from separate pull-down experiments. * antibody light chain. ( D ) Competitive cleavage of WT C100-FLAG by WT C100-Myc or V50F-M51F C100-Myc. ( E ) Aβ42/40 ratio of the V50F-M51F double mutant. Mean ± SD, n = 3, t-test, ****
    γ Secretase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/γ secretase/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    γ secretase - by Bioz Stars, 2020-04
    92/100 stars
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    93
    Bio-Rad chemokine concentrations
    Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and <t>chemokine</t> concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.
    Chemokine Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad ikbke total cell protein concentrations
    A. <t>IKBKe</t> transcription in <t>MDA-MB-231</t> cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p
    Ikbke Total Cell Protein Concentrations, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ikbke total cell protein concentrations - by Bioz Stars, 2020-04
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    Phenylalanine blocking mutations at both ε cleavage sites reduces APP cleavage but not binding to γ-secretase. ( A ) Western blot of γ-secretase cleavage of WT, V50F, M51F and V50F-M51F C100-FLAG. Duplicates from each substrate represent separate independent data points. * denotes a degradation product which co-purified with the substrate. ( B ) Cleavage of WT and V50F-M51F C100-FLAG over time. ( C ) Co-immunoprecipitation of Myc-tagged WT or V50F-M51F C100 substrate. Duplicates are from separate pull-down experiments. * antibody light chain. ( D ) Competitive cleavage of WT C100-FLAG by WT C100-Myc or V50F-M51F C100-Myc. ( E ) Aβ42/40 ratio of the V50F-M51F double mutant. Mean ± SD, n = 3, t-test, ****

    Journal: eLife

    Article Title: The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase

    doi: 10.7554/eLife.17578

    Figure Lengend Snippet: Phenylalanine blocking mutations at both ε cleavage sites reduces APP cleavage but not binding to γ-secretase. ( A ) Western blot of γ-secretase cleavage of WT, V50F, M51F and V50F-M51F C100-FLAG. Duplicates from each substrate represent separate independent data points. * denotes a degradation product which co-purified with the substrate. ( B ) Cleavage of WT and V50F-M51F C100-FLAG over time. ( C ) Co-immunoprecipitation of Myc-tagged WT or V50F-M51F C100 substrate. Duplicates are from separate pull-down experiments. * antibody light chain. ( D ) Competitive cleavage of WT C100-FLAG by WT C100-Myc or V50F-M51F C100-Myc. ( E ) Aβ42/40 ratio of the V50F-M51F double mutant. Mean ± SD, n = 3, t-test, ****

    Article Snippet: In vitro γ-secretase assay Purified γ-secretase was incorporated into vesicles by first dissolving total brain lipid extract (1.25 mM final) in 50 mM HEPES pH 7.0, 150 mM NaCl, 0.25% CHAPSO. γ-Secretase (5–30 nM final concentration) was then added to the solution and detergent removed by mixing SM-2 biobeads (62 mg/mL) (Bio-Rad) with the lipid/detergent/enzyme solution for two hrs at 4°C.

    Techniques: Blocking Assay, Binding Assay, Western Blot, Purification, Immunoprecipitation, Mutagenesis

    Tripeptide fragments of APP inhibit γ-secretase. ( A ) Schematic diagram of the major sequential cleavage pathways of the transmembrane domain of APP (Aβ49 → Aβ46 → Aβ43 → Aβ40 in red and Aβ48 → Aβ45 → Aβ42 in blue). Mutations causing Familial Alzheimer’s disease are below the APP TMD in blue. ( B ) IC50 curves from the inhibition of γ-secretase activity by APP product tripeptide fragments. Mean ± SD, n = 2. ( C ) Noncompetitive inhibition of γ-secretase with VIV tripeptide, R 2 = 0.98. ( D ) Yonetani-Theorell plot for the mutually exclusive binding of VIV and the noncompetitive transition-state analog inhibitor III-31-C, R 2 = 0.98. ( E ) Cartoon representation of the three S’ pockets of presenilin (PSEN) along with three P’ amino acids of substrate and the transition-state analog L685,458. ( F ) IC50 curves from the inhibition of γ-secretase activity with FAF and AFA synthetic tripeptides. Mean ± SD, n = 2. DOI: http://dx.doi.org/10.7554/eLife.17578.003

    Journal: eLife

    Article Title: The amyloid-beta forming tripeptide cleavage mechanism of γ-secretase

    doi: 10.7554/eLife.17578

    Figure Lengend Snippet: Tripeptide fragments of APP inhibit γ-secretase. ( A ) Schematic diagram of the major sequential cleavage pathways of the transmembrane domain of APP (Aβ49 → Aβ46 → Aβ43 → Aβ40 in red and Aβ48 → Aβ45 → Aβ42 in blue). Mutations causing Familial Alzheimer’s disease are below the APP TMD in blue. ( B ) IC50 curves from the inhibition of γ-secretase activity by APP product tripeptide fragments. Mean ± SD, n = 2. ( C ) Noncompetitive inhibition of γ-secretase with VIV tripeptide, R 2 = 0.98. ( D ) Yonetani-Theorell plot for the mutually exclusive binding of VIV and the noncompetitive transition-state analog inhibitor III-31-C, R 2 = 0.98. ( E ) Cartoon representation of the three S’ pockets of presenilin (PSEN) along with three P’ amino acids of substrate and the transition-state analog L685,458. ( F ) IC50 curves from the inhibition of γ-secretase activity with FAF and AFA synthetic tripeptides. Mean ± SD, n = 2. DOI: http://dx.doi.org/10.7554/eLife.17578.003

    Article Snippet: In vitro γ-secretase assay Purified γ-secretase was incorporated into vesicles by first dissolving total brain lipid extract (1.25 mM final) in 50 mM HEPES pH 7.0, 150 mM NaCl, 0.25% CHAPSO. γ-Secretase (5–30 nM final concentration) was then added to the solution and detergent removed by mixing SM-2 biobeads (62 mg/mL) (Bio-Rad) with the lipid/detergent/enzyme solution for two hrs at 4°C.

    Techniques: Inhibition, Activity Assay, Binding Assay

    Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Journal: PLoS ONE

    Article Title: Attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice

    doi: 10.1371/journal.pone.0196037

    Figure Lengend Snippet: Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Article Snippet: The protein concentration of the resulting supernatant was determined with a DC (detergent-compatible) protein assay (Bio-Rad, Hercules, CA), and the supernatant was then stored at –80°C until assay of cytokine and chemokine concentrations with the use of a Bio-Plex Pro Mouse Cytokine 23-Plex Panel and Bio-Plex Manager software version 4.1.1 (Bio-Rad).

    Techniques: Mouse Assay, Gas Chromatography, Immunohistofluorescence

    Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Journal: PLoS ONE

    Article Title: Attenuation of choroidal neovascularization by dietary intake of ω-3 long-chain polyunsaturated fatty acids and lutein in mice

    doi: 10.1371/journal.pone.0196037

    Figure Lengend Snippet: Experimental design. Mice maintained on a normal diet up to 6 weeks of age were then fed a diet either supplemented with ω-3 LCPUFAs ( n = 80) or free of ω-3 LCPUFAs ( n = 80) beginning 2 weeks before the induction of CNV. Half of the mice on each diet received lutein daily via an oral gavage needle beginning 1 week before the induction of CNV. The animals were killed 7 days after laser photocoagulation for measurement of CNV size ( n = 15 for each group [ n = 5 for each of three separate experiments), serum fatty acid and lutein concentrations ( n = 5 for each group from among the 15 mice examined for measurement of CNV size, and one sample was used for the calibration of gas chromatography), and cytokine and chemokine concentrations in the retina and choroid ( n = 5 for each group) as well as for immunoblot analysis of Nox4 ( n = 5 for each group) and immunohistofluorescence analysis of Nox4 ( n = 5 for each group). For detection of ROS ( n = 10 for each group [ n = 5 for each of two separate experiments]), the animals were killed 5 days after laser photocoagulation.

    Article Snippet: The protein concentration of the resulting supernatant was determined with a DC (detergent-compatible) protein assay (Bio-Rad, Hercules, CA), and the supernatant was then stored at –80°C until assay of cytokine and chemokine concentrations with the use of a Bio-Plex Pro Mouse Cytokine 23-Plex Panel and Bio-Plex Manager software version 4.1.1 (Bio-Rad).

    Techniques: Mouse Assay, Gas Chromatography, Immunohistofluorescence

    A. IKBKe transcription in MDA-MB-231 cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p

    Journal: PLoS ONE

    Article Title: Apigenin inhibits TNFα/IL-1α-induced CCL2 release through IKBK-epsilon signaling in MDA-MB-231 human breast cancer cells

    doi: 10.1371/journal.pone.0175558

    Figure Lengend Snippet: A. IKBKe transcription in MDA-MB-231 cells ± TNFα ± Apigenin. The data represent normalized expression and are expressed as the Mean ± S.E.M., n = 3. The significance of differences between the Ctrl and TNFα groups and TNFα vs. TNFα + Apigenin were determined by a Students t-test *p

    Article Snippet: Western blot ERK1/2 and IKBKe Total cell protein concentrations from MDA-MB-231 cells treated with apigenin, with and without TNFα co-treatment, was determined using a modified Bio-Rad “DC” protein assay (Bio-Rad Laboratories, Hercules, CA, USA).

    Techniques: Multiple Displacement Amplification, Expressing