protease inhibitor  (Millipore)


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  • 99
    Name:
    Protease Inhibitor Mix
    Description:
    Protease inhibitor mix
    Catalog Number:
    ge80-6501-23
    Price:
    None
    Applications:
    Sample preparation often requires the inhibition of protease activity. GE Healthcare offers this combination of competitive and noncompetitive protease inhibitors, which protect Proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.
    Buy from Supplier


    Structured Review

    Millipore protease inhibitor
    Protease Inhibitor Mix
    Protease inhibitor mix
    https://www.bioz.com/result/protease inhibitor/product/Millipore
    Average 99 stars, based on 687 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Strategy and rationale for urine collection protocols employed in the NEPTUNE study
    Article Snippet: .. There was substantially better detection of the podocyte marker of interest PODXL in cellular pellets stored with protease inhibitors, and again found that Sigma protease inhibitor product may in fact be superior to the more expensive Roche protease inhibitor cocktail (Additional file : Figure S1). .. Without the presence of protease inhibitor, the proteins we examined (podocalyxin, fibrocystin and smoothened) did not survive 12 months of storage (6 month representative Western shown), or a freeze/thaw cycle at −80 °C.

    Article Title: C-terminomics Screen for Natural Substrates of Cytosolic Carboxypeptidase 1 Reveals Processing of Acidic Protein C termini *
    Article Snippet: .. Affinity purified CCP1 was incubated at 5 ng/μl for 2 h or 16 h at 37 °C with affinity purified TRAD1 or HMGB3 at 25 ng/μl in CCP1 buffer (80 m m PIPES, pH 6.8, 1 m m MgCl2 , complemented with EDTA-free protease inhibitor mixture Set III (EMD Millipore)). ..

    Article Title: Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26
    Article Snippet: .. For denatured immunoprecipitation, cell pellets were lysed in SDS lysis buffer [1% SDS in TBS (10mM Tris-HCl, pH 7.5, 150mM NaCl, and protease inhibitor mix (Sigma)] by consecutive vigorous vortexing and boiling. .. After addition of two volumes of 1.5% triton X-100 in TBS, lysates were cleared by centrifugation and supernatants were added to protein A mix prepared in advance (see “co-immunoprecipitation”).

    Article Title: Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria *
    Article Snippet: .. Protein A-conjugated agarose beads (AffiGel; Bio-Rad) were incubated with either control mouse Ig or anti-EHD3 Ig in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Article Title: SynGAP isoforms differentially regulate synaptic plasticity and dendritic development
    Article Snippet: .. In brief, mouse brains were collected and homogenized by 10–15 strokes of a Dounce A homogenizer in Buffer A (0.32M Sucrose, 10 mM Hepes (pH7.4) with cOmplete protease inhibitor mix (SIGMA)). ..

    Article Title: Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells
    Article Snippet: .. Western blot analysisCells were lysed with EBC buffer (50 mM Tris-HCl, pH 8.0, 120 mM NaCl, 1% NP-40, and 1 mM ethylenediaminetetraacetic acid; EDTA) supplemented with PhosSTOP phosphatase inhibitor and cOmplete protease inhibitor (MilliporeSigma, Oakville, Canada). ..

    Article Title: Autophagic Inhibition via Lysosomal Integrity Dysfunction Leads to Antitumor Activity in Glioma Treatment
    Article Snippet: .. Bafilomycin A1, rapamycin, dithiothreitol (DTT), trifluoperazine, iodoacetamide (IAA), formic acid, E64D cysteine protease inhibitor, pifithrin-μ (PES), and artesunate (ART) were purchased from Sigma-Aldrich (St. Louis, MO, USA). .. Pronase and protease inhibitor cocktail tablets were obtained from Roche (Basel, Switzerland).

    Immunoprecipitation:

    Article Title: Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26
    Article Snippet: .. For denatured immunoprecipitation, cell pellets were lysed in SDS lysis buffer [1% SDS in TBS (10mM Tris-HCl, pH 7.5, 150mM NaCl, and protease inhibitor mix (Sigma)] by consecutive vigorous vortexing and boiling. .. After addition of two volumes of 1.5% triton X-100 in TBS, lysates were cleared by centrifugation and supernatants were added to protein A mix prepared in advance (see “co-immunoprecipitation”).

    Incubation:

    Article Title: C-terminomics Screen for Natural Substrates of Cytosolic Carboxypeptidase 1 Reveals Processing of Acidic Protein C termini *
    Article Snippet: .. Affinity purified CCP1 was incubated at 5 ng/μl for 2 h or 16 h at 37 °C with affinity purified TRAD1 or HMGB3 at 25 ng/μl in CCP1 buffer (80 m m PIPES, pH 6.8, 1 m m MgCl2 , complemented with EDTA-free protease inhibitor mixture Set III (EMD Millipore)). ..

    Article Title: Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria *
    Article Snippet: .. Protein A-conjugated agarose beads (AffiGel; Bio-Rad) were incubated with either control mouse Ig or anti-EHD3 Ig in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Lysis:

    Article Title: Mdm2 regulates p53 mRNA translation through inhibitory interactions with ribosomal protein L26
    Article Snippet: .. For denatured immunoprecipitation, cell pellets were lysed in SDS lysis buffer [1% SDS in TBS (10mM Tris-HCl, pH 7.5, 150mM NaCl, and protease inhibitor mix (Sigma)] by consecutive vigorous vortexing and boiling. .. After addition of two volumes of 1.5% triton X-100 in TBS, lysates were cleared by centrifugation and supernatants were added to protein A mix prepared in advance (see “co-immunoprecipitation”).

    Marker:

    Article Title: Strategy and rationale for urine collection protocols employed in the NEPTUNE study
    Article Snippet: .. There was substantially better detection of the podocyte marker of interest PODXL in cellular pellets stored with protease inhibitors, and again found that Sigma protease inhibitor product may in fact be superior to the more expensive Roche protease inhibitor cocktail (Additional file : Figure S1). .. Without the presence of protease inhibitor, the proteins we examined (podocalyxin, fibrocystin and smoothened) did not survive 12 months of storage (6 month representative Western shown), or a freeze/thaw cycle at −80 °C.

    Western Blot:

    Article Title: Dianhydrogalactitol synergizes with topoisomerase poisons to overcome DNA repair activity in tumor cells
    Article Snippet: .. Western blot analysisCells were lysed with EBC buffer (50 mM Tris-HCl, pH 8.0, 120 mM NaCl, 1% NP-40, and 1 mM ethylenediaminetetraacetic acid; EDTA) supplemented with PhosSTOP phosphatase inhibitor and cOmplete protease inhibitor (MilliporeSigma, Oakville, Canada). ..

    Affinity Purification:

    Article Title: C-terminomics Screen for Natural Substrates of Cytosolic Carboxypeptidase 1 Reveals Processing of Acidic Protein C termini *
    Article Snippet: .. Affinity purified CCP1 was incubated at 5 ng/μl for 2 h or 16 h at 37 °C with affinity purified TRAD1 or HMGB3 at 25 ng/μl in CCP1 buffer (80 m m PIPES, pH 6.8, 1 m m MgCl2 , complemented with EDTA-free protease inhibitor mixture Set III (EMD Millipore)). ..

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Binding Assay:

    Article Title: Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria *
    Article Snippet: .. Protein A-conjugated agarose beads (AffiGel; Bio-Rad) were incubated with either control mouse Ig or anti-EHD3 Ig in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *
    Article Snippet: .. Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C. ..

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  • 88
    Millipore parg inhibitor adp hpd
    PTEN is <t>ADP-ribosylated</t> by tankyrases in vitro and in vivo. ( A , B ) PTEN is PARylated in vivo. ( A ) HCT116 cells were lysed with NETN buffer containing <t>PARG</t> inhibitor <t>ADP-HPD</t> (5 μM) and protease inhibitor. Lysates were then immunoprecipitated using control or anti-PAR antibodies and immunoblotted using the indicated antibodies. ( B ) HCT116 cells were lysed with NETN denaturing buffer containing PARG inhibitor ADP-HPD (5 μM) and protease inhibitor. Lysates were then immunoprecipitated using control or anti-PTEN antibodies followed by Western blotting as indicated. ( C ) Ribosylation of PTEN by TNKS1 and TNKS2 in vitro. Recombinant TNKS1, TNKS2, and PTEN were subjected to in vitro ribosylation assays in the absence or presence of biotin-labeled NAD + . The recombinant proteins were detected by the indicated antibodies, and the ribosylated proteins were determined with anti-biotin antibody. ( D ) The ribosylation of PTEN by TNKS1 is diminished by tankyrase inhibitor XAV939. The recombinant MBP-PTEN and TNKS1 were subjected to an in vitro ribosylation reaction as described above in the absence or presence of the indicated concentrations of XAV939. ( E ) The catalytic activity of TNKS1 is required for PTEN ribosylation. The MBP-PTEN and immunoprecipitated SFB-tagged wild-type or the catalytically inactive mutant of TNKS1 (TNKS1-PD) were subjected to an in vitro ribosylation reaction followed by Western blotting as indicated. ( F ) The tankyrase-binding motif of PTEN is required for its ribosylation by TNKS1. The recombinant MBP-PTEN, MBP-PTEN-AA, and TNKS1 were subjected to an in vitro ribosylation assay and analyzed by Western blotting as indicated. ( G ) Only double knockdown of TNKS1/2 diminishes the ribosylation of PTEN in vivo. HCT116-derived cells with stable knockdown of TNKS1, TNKS2, TNKS1/2, or PTEN were collected and immunoprecipitated using anti-PTEN antibody. The input and immunoprecipitated proteins were analyzed by Western blotting using the indicated antibodies, and the ribosylation of endogenous PTEN was detected by anti-PAR antibody.
    Parg Inhibitor Adp Hpd, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 3 article reviews
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    94
    Millipore dmso
    <t>TBZ</t> inhibits angiogenesis in vivo in Xenopus embryos. Formation of Xenopus embryo veins is disrupted, marked by expression of vascular reporter genes (A, B) erg and (C, D) aplnr , contrasting treatment with 1% <t>DMSO</t> control only (A, C) with 1% DMSO, 250 µM TBZ, treated at stage 31 and imaged at stages 35–36 (B, D). PCV, posterior cardinal vein; ISV, intersomitic vein; VV, vitellin veins. Similarly, TBZ disrupts vasculature imaged within a living Xenopus embryo and visualized by vascular specific GFP in kdr:GFP frogs from [19] , contrasting the vasculature of stage 46 animals treated from stage 41 with the 1% DMSO control (E) or 1% DMSO, 250 µM TBZ (F). Scale bar, 200 µm.
    Dmso, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmso/product/Millipore
    Average 94 stars, based on 162 article reviews
    Price from $9.99 to $1999.99
    dmso - by Bioz Stars, 2020-11
    94/100 stars
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    85
    Millipore glutathionylation assay thoraces
    DmGSTO1 partially restored mitochondrial F 1 F 0 -ATP synthase activity in park 1 mutants. A , in the presence of GSH, recombinant ATP synthase β subunit was glutathionylated by DmGSTO1A in a dose-dependent manner. B , glutathionylated proteins were immunoprecipitated from thorax extracts with an anti-GSH antibody and were immunoblotted with an anti-ATP synthase β antibody. <t>Glutathionylation</t> of endogenous ATP synthase β subunit in park 1 mutants was regulated by the GSH-conjugating catalytic activity of DmGSTO1A but not by DmGSTO1B. The endogenous levels of the glutathionylated form of the ATP synthase β subunit were decreased even more in park 1 /DmGSTO1 null double mutants. Error bars , S.D. The experimental significance was determined by one-way ANOVA (*, p
    Glutathionylation Assay Thoraces, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutathionylation assay thoraces/product/Millipore
    Average 85 stars, based on 4 article reviews
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    86
    Millipore caco3 dox loaded nanocrystal
    (a) is a control group without treatment while (b), (c), and (d) were treated with CaCO 3 <t>/Dox</t> <t>nanocrystal</t> for 24, 48, and 72 hr, respectively.
    Caco3 Dox Loaded Nanocrystal, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PTEN is ADP-ribosylated by tankyrases in vitro and in vivo. ( A , B ) PTEN is PARylated in vivo. ( A ) HCT116 cells were lysed with NETN buffer containing PARG inhibitor ADP-HPD (5 μM) and protease inhibitor. Lysates were then immunoprecipitated using control or anti-PAR antibodies and immunoblotted using the indicated antibodies. ( B ) HCT116 cells were lysed with NETN denaturing buffer containing PARG inhibitor ADP-HPD (5 μM) and protease inhibitor. Lysates were then immunoprecipitated using control or anti-PTEN antibodies followed by Western blotting as indicated. ( C ) Ribosylation of PTEN by TNKS1 and TNKS2 in vitro. Recombinant TNKS1, TNKS2, and PTEN were subjected to in vitro ribosylation assays in the absence or presence of biotin-labeled NAD + . The recombinant proteins were detected by the indicated antibodies, and the ribosylated proteins were determined with anti-biotin antibody. ( D ) The ribosylation of PTEN by TNKS1 is diminished by tankyrase inhibitor XAV939. The recombinant MBP-PTEN and TNKS1 were subjected to an in vitro ribosylation reaction as described above in the absence or presence of the indicated concentrations of XAV939. ( E ) The catalytic activity of TNKS1 is required for PTEN ribosylation. The MBP-PTEN and immunoprecipitated SFB-tagged wild-type or the catalytically inactive mutant of TNKS1 (TNKS1-PD) were subjected to an in vitro ribosylation reaction followed by Western blotting as indicated. ( F ) The tankyrase-binding motif of PTEN is required for its ribosylation by TNKS1. The recombinant MBP-PTEN, MBP-PTEN-AA, and TNKS1 were subjected to an in vitro ribosylation assay and analyzed by Western blotting as indicated. ( G ) Only double knockdown of TNKS1/2 diminishes the ribosylation of PTEN in vivo. HCT116-derived cells with stable knockdown of TNKS1, TNKS2, TNKS1/2, or PTEN were collected and immunoprecipitated using anti-PTEN antibody. The input and immunoprecipitated proteins were analyzed by Western blotting using the indicated antibodies, and the ribosylation of endogenous PTEN was detected by anti-PAR antibody.

    Journal: Genes & Development

    Article Title: Poly-ADP ribosylation of PTEN by tankyrases promotes PTEN degradation and tumor growth

    doi: 10.1101/gad.251785.114

    Figure Lengend Snippet: PTEN is ADP-ribosylated by tankyrases in vitro and in vivo. ( A , B ) PTEN is PARylated in vivo. ( A ) HCT116 cells were lysed with NETN buffer containing PARG inhibitor ADP-HPD (5 μM) and protease inhibitor. Lysates were then immunoprecipitated using control or anti-PAR antibodies and immunoblotted using the indicated antibodies. ( B ) HCT116 cells were lysed with NETN denaturing buffer containing PARG inhibitor ADP-HPD (5 μM) and protease inhibitor. Lysates were then immunoprecipitated using control or anti-PTEN antibodies followed by Western blotting as indicated. ( C ) Ribosylation of PTEN by TNKS1 and TNKS2 in vitro. Recombinant TNKS1, TNKS2, and PTEN were subjected to in vitro ribosylation assays in the absence or presence of biotin-labeled NAD + . The recombinant proteins were detected by the indicated antibodies, and the ribosylated proteins were determined with anti-biotin antibody. ( D ) The ribosylation of PTEN by TNKS1 is diminished by tankyrase inhibitor XAV939. The recombinant MBP-PTEN and TNKS1 were subjected to an in vitro ribosylation reaction as described above in the absence or presence of the indicated concentrations of XAV939. ( E ) The catalytic activity of TNKS1 is required for PTEN ribosylation. The MBP-PTEN and immunoprecipitated SFB-tagged wild-type or the catalytically inactive mutant of TNKS1 (TNKS1-PD) were subjected to an in vitro ribosylation reaction followed by Western blotting as indicated. ( F ) The tankyrase-binding motif of PTEN is required for its ribosylation by TNKS1. The recombinant MBP-PTEN, MBP-PTEN-AA, and TNKS1 were subjected to an in vitro ribosylation assay and analyzed by Western blotting as indicated. ( G ) Only double knockdown of TNKS1/2 diminishes the ribosylation of PTEN in vivo. HCT116-derived cells with stable knockdown of TNKS1, TNKS2, TNKS1/2, or PTEN were collected and immunoprecipitated using anti-PTEN antibody. The input and immunoprecipitated proteins were analyzed by Western blotting using the indicated antibodies, and the ribosylation of endogenous PTEN was detected by anti-PAR antibody.

    Article Snippet: Cycloheximide, MG132, Wiki4, puromycin, G418, and doxycycline were purchased from Sigma-Aldrich; PAPR1/2 inhibitor Olaparib was from LC Laboratories; PARG inhibitor ADP-HPD and JW55 were from Millipore; and XAV939 was from Sigma-Aldrich and Selleckchem.

    Techniques: In Vitro, In Vivo, Protease Inhibitor, Immunoprecipitation, Western Blot, Recombinant, Labeling, Activity Assay, Mutagenesis, Binding Assay, Derivative Assay

    TBZ inhibits angiogenesis in vivo in Xenopus embryos. Formation of Xenopus embryo veins is disrupted, marked by expression of vascular reporter genes (A, B) erg and (C, D) aplnr , contrasting treatment with 1% DMSO control only (A, C) with 1% DMSO, 250 µM TBZ, treated at stage 31 and imaged at stages 35–36 (B, D). PCV, posterior cardinal vein; ISV, intersomitic vein; VV, vitellin veins. Similarly, TBZ disrupts vasculature imaged within a living Xenopus embryo and visualized by vascular specific GFP in kdr:GFP frogs from [19] , contrasting the vasculature of stage 46 animals treated from stage 41 with the 1% DMSO control (E) or 1% DMSO, 250 µM TBZ (F). Scale bar, 200 µm.

    Journal: PLoS Biology

    Article Title: Evolutionarily Repurposed Networks Reveal the Well-Known Antifungal Drug Thiabendazole to Be a Novel Vascular Disrupting AgentEvolution-Based Drug Discovery: Antifungal Also Disrupts Blood Vessel Formation

    doi: 10.1371/journal.pbio.1001379

    Figure Lengend Snippet: TBZ inhibits angiogenesis in vivo in Xenopus embryos. Formation of Xenopus embryo veins is disrupted, marked by expression of vascular reporter genes (A, B) erg and (C, D) aplnr , contrasting treatment with 1% DMSO control only (A, C) with 1% DMSO, 250 µM TBZ, treated at stage 31 and imaged at stages 35–36 (B, D). PCV, posterior cardinal vein; ISV, intersomitic vein; VV, vitellin veins. Similarly, TBZ disrupts vasculature imaged within a living Xenopus embryo and visualized by vascular specific GFP in kdr:GFP frogs from [19] , contrasting the vasculature of stage 46 animals treated from stage 41 with the 1% DMSO control (E) or 1% DMSO, 250 µM TBZ (F). Scale bar, 200 µm.

    Article Snippet: Mass Spectrometry HUVECs were treated with 1% DMSO or 1% DMSO, 250 µM TBZ for 24 h, and lysed by Dounce homogenization in low salt buffer (10 mM Tris-HCl, pH 8.8, 10 mM KCl, 1.5 mM MgCl2 ) with 0.5 mM DTT and protease inhibitor mixture (Calbiochem).

    Techniques: In Vivo, Expressing

    TBZ significantly disrupts tube formation in cultured human umbilical vein endothelial cells (HUVECs), an in vitro capillary model. Here, we show effects of 1% DMSO-treated control (A) versus 1% DMSO, 100 µM TBZ (B) and 1% DMSO, 250 µM TBZ (C). Scale bar, 100 µm. (D) Tube disruption is dose-dependent and comparable to that from silencing known pro-angiogenic gene HOXA9 .

    Journal: PLoS Biology

    Article Title: Evolutionarily Repurposed Networks Reveal the Well-Known Antifungal Drug Thiabendazole to Be a Novel Vascular Disrupting AgentEvolution-Based Drug Discovery: Antifungal Also Disrupts Blood Vessel Formation

    doi: 10.1371/journal.pbio.1001379

    Figure Lengend Snippet: TBZ significantly disrupts tube formation in cultured human umbilical vein endothelial cells (HUVECs), an in vitro capillary model. Here, we show effects of 1% DMSO-treated control (A) versus 1% DMSO, 100 µM TBZ (B) and 1% DMSO, 250 µM TBZ (C). Scale bar, 100 µm. (D) Tube disruption is dose-dependent and comparable to that from silencing known pro-angiogenic gene HOXA9 .

    Article Snippet: Mass Spectrometry HUVECs were treated with 1% DMSO or 1% DMSO, 250 µM TBZ for 24 h, and lysed by Dounce homogenization in low salt buffer (10 mM Tris-HCl, pH 8.8, 10 mM KCl, 1.5 mM MgCl2 ) with 0.5 mM DTT and protease inhibitor mixture (Calbiochem).

    Techniques: Cell Culture, In Vitro

    TBZ impedes migration of HUVECs in a wound scratch assay, but treatment with the Rho Kinase inhibitor Y27632 reverses TBZ's effects. (A) The effects of 1% DMSO-treated control versus 1% DMSO, 250 µM TBZ, and 1% DMSO, 250 µM TBZ, 10 µM Y27632. Scale bar, 200 µm. (B) quantifies the dose-dependent suppression of TBZ inhibition by Y27632. Error bars represent the mean ± 1 s.d. across 3 wells (1 of 3 trials). TBZ results in disorganization of actin stress fibers, as shown in (C) for 1% DMSO-treated control versus 1% DMSO, 250 µM TBZ-treated cells. Scale bar, 20 µm.

    Journal: PLoS Biology

    Article Title: Evolutionarily Repurposed Networks Reveal the Well-Known Antifungal Drug Thiabendazole to Be a Novel Vascular Disrupting AgentEvolution-Based Drug Discovery: Antifungal Also Disrupts Blood Vessel Formation

    doi: 10.1371/journal.pbio.1001379

    Figure Lengend Snippet: TBZ impedes migration of HUVECs in a wound scratch assay, but treatment with the Rho Kinase inhibitor Y27632 reverses TBZ's effects. (A) The effects of 1% DMSO-treated control versus 1% DMSO, 250 µM TBZ, and 1% DMSO, 250 µM TBZ, 10 µM Y27632. Scale bar, 200 µm. (B) quantifies the dose-dependent suppression of TBZ inhibition by Y27632. Error bars represent the mean ± 1 s.d. across 3 wells (1 of 3 trials). TBZ results in disorganization of actin stress fibers, as shown in (C) for 1% DMSO-treated control versus 1% DMSO, 250 µM TBZ-treated cells. Scale bar, 20 µm.

    Article Snippet: Mass Spectrometry HUVECs were treated with 1% DMSO or 1% DMSO, 250 µM TBZ for 24 h, and lysed by Dounce homogenization in low salt buffer (10 mM Tris-HCl, pH 8.8, 10 mM KCl, 1.5 mM MgCl2 ) with 0.5 mM DTT and protease inhibitor mixture (Calbiochem).

    Techniques: Migration, Wound Healing Assay, Inhibition

    DmGSTO1 partially restored mitochondrial F 1 F 0 -ATP synthase activity in park 1 mutants. A , in the presence of GSH, recombinant ATP synthase β subunit was glutathionylated by DmGSTO1A in a dose-dependent manner. B , glutathionylated proteins were immunoprecipitated from thorax extracts with an anti-GSH antibody and were immunoblotted with an anti-ATP synthase β antibody. Glutathionylation of endogenous ATP synthase β subunit in park 1 mutants was regulated by the GSH-conjugating catalytic activity of DmGSTO1A but not by DmGSTO1B. The endogenous levels of the glutathionylated form of the ATP synthase β subunit were decreased even more in park 1 /DmGSTO1 null double mutants. Error bars , S.D. The experimental significance was determined by one-way ANOVA (*, p

    Journal: The Journal of Biological Chemistry

    Article Title: Glutathione S-Transferase Omega 1 Activity Is Sufficient to Suppress Neurodegeneration in a Drosophila Model of Parkinson Disease *

    doi: 10.1074/jbc.M111.291179

    Figure Lengend Snippet: DmGSTO1 partially restored mitochondrial F 1 F 0 -ATP synthase activity in park 1 mutants. A , in the presence of GSH, recombinant ATP synthase β subunit was glutathionylated by DmGSTO1A in a dose-dependent manner. B , glutathionylated proteins were immunoprecipitated from thorax extracts with an anti-GSH antibody and were immunoblotted with an anti-ATP synthase β antibody. Glutathionylation of endogenous ATP synthase β subunit in park 1 mutants was regulated by the GSH-conjugating catalytic activity of DmGSTO1A but not by DmGSTO1B. The endogenous levels of the glutathionylated form of the ATP synthase β subunit were decreased even more in park 1 /DmGSTO1 null double mutants. Error bars , S.D. The experimental significance was determined by one-way ANOVA (*, p

    Article Snippet: Immunoprecipitation and Glutathionylation Assay Thoraces from 3-day-old male flies were homogenized in lysis buffer containing 1× protease inhibitor mixture (Calbiochem-Merck4Biosciences).

    Techniques: Activity Assay, Recombinant, Immunoprecipitation

    (a) is a control group without treatment while (b), (c), and (d) were treated with CaCO 3 /Dox nanocrystal for 24, 48, and 72 hr, respectively.

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: (a) is a control group without treatment while (b), (c), and (d) were treated with CaCO 3 /Dox nanocrystal for 24, 48, and 72 hr, respectively.

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques:

    TUNEL DNA fragmentation assay on MCF-7 cells after treatment with Dox loaded CaCO 3 nanocrystal. (a) is untreated cells showing complete absent of green fluorescence colour while (b), (c), and (d) are treated cells for incubation period of 24, 48, and 72 h, respectively. The cells showed depicting of TUNEL positive stain progressively according to the duration period.

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: TUNEL DNA fragmentation assay on MCF-7 cells after treatment with Dox loaded CaCO 3 nanocrystal. (a) is untreated cells showing complete absent of green fluorescence colour while (b), (c), and (d) are treated cells for incubation period of 24, 48, and 72 h, respectively. The cells showed depicting of TUNEL positive stain progressively according to the duration period.

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques: TUNEL Assay, DNA Fragmentation Assay, Fluorescence, Incubation, Staining

    In vitro cytotoxicity study of MCF-7 cells for incubation periods of 24, 48, and 72 hr with free Dox and the CaCO 3 /Dox nanocrystals. ∗Means with different superscript are statistically significant P

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: In vitro cytotoxicity study of MCF-7 cells for incubation periods of 24, 48, and 72 hr with free Dox and the CaCO 3 /Dox nanocrystals. ∗Means with different superscript are statistically significant P

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques: In Vitro, Incubation

    FTIR spectra of CaCO 3 nanocrystal, CaCO 3 /Dox loaded nanocrystal, and free Dox.

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: FTIR spectra of CaCO 3 nanocrystal, CaCO 3 /Dox loaded nanocrystal, and free Dox.

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques:

    Apoptotic index MCF-7 cells treated with CaCO 3 /Dox nanocrystal. ∗Means with different superscript are statistically significant P

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: Apoptotic index MCF-7 cells treated with CaCO 3 /Dox nanocrystal. ∗Means with different superscript are statistically significant P

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques:

    Inverted light microscope images of morphological changes on MCF-7 treated with Dox nanocrystals.

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: Inverted light microscope images of morphological changes on MCF-7 treated with Dox nanocrystals.

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques: Light Microscopy

    In vitro cytotoxicity study of MCF-7 cells for incubation periods of 24, 48, and 72 hr with free DOX and the CaCO 3 /Dox nanocrystals. ∗Means with different superscript are statistically significant P

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: In vitro cytotoxicity study of MCF-7 cells for incubation periods of 24, 48, and 72 hr with free DOX and the CaCO 3 /Dox nanocrystals. ∗Means with different superscript are statistically significant P

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques: In Vitro, Incubation

    (a) TEM micrograph of the Dox-loaded rod-shape nanocrystals and (b) FESEM micrograph of CaCO 3 /Dox nanocrystals.

    Journal: BioMed Research International

    Article Title: In Vitro Ultrastructural Changes of MCF-7 for Metastasise Bone Cancer and Induction of Apoptosis via Mitochondrial Cytochrome C Released by CaCO3/Dox Nanocrystals

    doi: 10.1155/2014/391869

    Figure Lengend Snippet: (a) TEM micrograph of the Dox-loaded rod-shape nanocrystals and (b) FESEM micrograph of CaCO 3 /Dox nanocrystals.

    Article Snippet: The cells were seeded at a density of 1 × 106 per dish and incubated overnight at 37°C; then the cells were treated with various concentrations of CaCO3 /Dox loaded nanocrystal and incubated for 24 h; total protein was extracted from the cells using lysis buffer-containing protease inhibitor cocktail set III and phosphatase inhibitor cocktail set I (Calbiochem, EMD Biosciences, San Diego, CA).

    Techniques: Transmission Electron Microscopy