protease inhibitor phenylmethylsulfonyl fluoride  (Millipore)


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    Name:
    PMSF
    Description:

    Catalog Number:
    pmsf-ro
    Price:
    None
    Applications:
    PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
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    Structured Review

    Millipore protease inhibitor phenylmethylsulfonyl fluoride
    PMSF

    https://www.bioz.com/result/protease inhibitor phenylmethylsulfonyl fluoride/product/Millipore
    Average 99 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor phenylmethylsulfonyl fluoride - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1
    Article Snippet: .. Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem). .. The gels were stained with Coomassie blue for 30 min and were destained with 30% methanol–10% acetic acid.

    Protease Inhibitor:

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells
    Article Snippet: .. Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]). .. Proteins were separated on 10% polyacrylamide gel and transferred to a PVFD membrane (Millipore).

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Neutrophil activation and arteritis induced by C. albicans water-soluble mannoprotein-β-glucan complex (CAWS)
    Article Snippet: .. Reagents ELISA kits for mouse IL-1β, IL-6, IL-10, IL-12 p70, IFN-γ, TNF-α were purchased from BD Biosciences (CA, USA), IL-18 was from Medical and Biological Laboratories (Nagoya, Japan), soluble ICAM-1 and MIP-2 were from R & D Systems (MN, USA), G-CSF and GM-CSF were from AN'ALYZA (MN, US). fMet-Leu-Phe (fMLP) was purchased from Peptide Institute (Osaka, Japan), 3,3′, 5,5′-tetrametylbendizine (TMB), cytochalasin B (CB), cytochrome c , RPMI 1640 medium, aprotinin and PMSF were purchased from Sigma Chemical Co. (MO, USA). ..

    Incubation:

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1
    Article Snippet: .. Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem). .. The gels were stained with Coomassie blue for 30 min and were destained with 30% methanol–10% acetic acid.

    Inhibition:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Activity Assay:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Lysis:

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells
    Article Snippet: .. Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]). .. Proteins were separated on 10% polyacrylamide gel and transferred to a PVFD membrane (Millipore).

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  • 99
    Millipore pmsf
    Expression of a 120-kDa gelatinase by Id-1-expressing mammary epithelial cells. (A) Gelatin zymogram of conditioned media from SCp2–Id-1A (lane 1) and SCp2–Id-1E (lane 2) cells. Cells were cultured in serum-free medium, and conditioned media were harvested and analyzed on a gelatin substrate gel, as described in Materials and Methods. (B) Gelatin zymogram of conditioned media from SCp2–Id-1A cells either growth arrested by serum deprivation (lane 1) or growing in 5% serum (lane 2). (C) Gelatin zymogram of conditioned media from control SCp2 cells (lane 1) and an uncloned SCp2–Id-1-transfected pooled population (lane 2). Cells were cultured in serum-free medium. (D) Gelatin zymogram of SCp2–Id-1A (lanes 1, 3, and 5) and SCp2–Id-1E (lanes 2, 4, and 6) cell-conditioned media incubated with <t>DMSO</t> (lanes 1 and 2), the MMP inhibitor GM6001 (0.2 mM in DMSO) (lanes 3 and 4), or the serine proteinase inhibitor <t>PMSF</t> (5 mM in DMSO) (lanes 5 and 6). (E) Casein zymogram of conditioned media from SCp2–Id-1A (lanes 1 and 3) and SCp2–Id-1E (lanes 2 and 4) cells incubated with DMSO (lanes 1 and 2) or GM6001 (lanes 3 and 4). In panels A through D, arrows mark the positions of the 120-kDa MMP.
    Pmsf, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmsf/product/Millipore
    Average 99 stars, based on 3381 article reviews
    Price from $9.99 to $1999.99
    pmsf - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    91
    Millipore m phenylmethylsulfonyl fluoride pmsf
    Expression of a 120-kDa gelatinase by Id-1-expressing mammary epithelial cells. (A) Gelatin zymogram of conditioned media from SCp2–Id-1A (lane 1) and SCp2–Id-1E (lane 2) cells. Cells were cultured in serum-free medium, and conditioned media were harvested and analyzed on a gelatin substrate gel, as described in Materials and Methods. (B) Gelatin zymogram of conditioned media from SCp2–Id-1A cells either growth arrested by serum deprivation (lane 1) or growing in 5% serum (lane 2). (C) Gelatin zymogram of conditioned media from control SCp2 cells (lane 1) and an uncloned SCp2–Id-1-transfected pooled population (lane 2). Cells were cultured in serum-free medium. (D) Gelatin zymogram of SCp2–Id-1A (lanes 1, 3, and 5) and SCp2–Id-1E (lanes 2, 4, and 6) cell-conditioned media incubated with <t>DMSO</t> (lanes 1 and 2), the MMP inhibitor GM6001 (0.2 mM in DMSO) (lanes 3 and 4), or the serine proteinase inhibitor <t>PMSF</t> (5 mM in DMSO) (lanes 5 and 6). (E) Casein zymogram of conditioned media from SCp2–Id-1A (lanes 1 and 3) and SCp2–Id-1E (lanes 2 and 4) cells incubated with DMSO (lanes 1 and 2) or GM6001 (lanes 3 and 4). In panels A through D, arrows mark the positions of the 120-kDa MMP.
    M Phenylmethylsulfonyl Fluoride Pmsf, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m phenylmethylsulfonyl fluoride pmsf/product/Millipore
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    m phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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    Expression of a 120-kDa gelatinase by Id-1-expressing mammary epithelial cells. (A) Gelatin zymogram of conditioned media from SCp2–Id-1A (lane 1) and SCp2–Id-1E (lane 2) cells. Cells were cultured in serum-free medium, and conditioned media were harvested and analyzed on a gelatin substrate gel, as described in Materials and Methods. (B) Gelatin zymogram of conditioned media from SCp2–Id-1A cells either growth arrested by serum deprivation (lane 1) or growing in 5% serum (lane 2). (C) Gelatin zymogram of conditioned media from control SCp2 cells (lane 1) and an uncloned SCp2–Id-1-transfected pooled population (lane 2). Cells were cultured in serum-free medium. (D) Gelatin zymogram of SCp2–Id-1A (lanes 1, 3, and 5) and SCp2–Id-1E (lanes 2, 4, and 6) cell-conditioned media incubated with DMSO (lanes 1 and 2), the MMP inhibitor GM6001 (0.2 mM in DMSO) (lanes 3 and 4), or the serine proteinase inhibitor PMSF (5 mM in DMSO) (lanes 5 and 6). (E) Casein zymogram of conditioned media from SCp2–Id-1A (lanes 1 and 3) and SCp2–Id-1E (lanes 2 and 4) cells incubated with DMSO (lanes 1 and 2) or GM6001 (lanes 3 and 4). In panels A through D, arrows mark the positions of the 120-kDa MMP.

    Journal: Molecular and Cellular Biology

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1

    doi:

    Figure Lengend Snippet: Expression of a 120-kDa gelatinase by Id-1-expressing mammary epithelial cells. (A) Gelatin zymogram of conditioned media from SCp2–Id-1A (lane 1) and SCp2–Id-1E (lane 2) cells. Cells were cultured in serum-free medium, and conditioned media were harvested and analyzed on a gelatin substrate gel, as described in Materials and Methods. (B) Gelatin zymogram of conditioned media from SCp2–Id-1A cells either growth arrested by serum deprivation (lane 1) or growing in 5% serum (lane 2). (C) Gelatin zymogram of conditioned media from control SCp2 cells (lane 1) and an uncloned SCp2–Id-1-transfected pooled population (lane 2). Cells were cultured in serum-free medium. (D) Gelatin zymogram of SCp2–Id-1A (lanes 1, 3, and 5) and SCp2–Id-1E (lanes 2, 4, and 6) cell-conditioned media incubated with DMSO (lanes 1 and 2), the MMP inhibitor GM6001 (0.2 mM in DMSO) (lanes 3 and 4), or the serine proteinase inhibitor PMSF (5 mM in DMSO) (lanes 5 and 6). (E) Casein zymogram of conditioned media from SCp2–Id-1A (lanes 1 and 3) and SCp2–Id-1E (lanes 2 and 4) cells incubated with DMSO (lanes 1 and 2) or GM6001 (lanes 3 and 4). In panels A through D, arrows mark the positions of the 120-kDa MMP.

    Article Snippet: Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem).

    Techniques: Expressing, Cell Culture, Transfection, Incubation

    Expression MosIR binding by dsRBPs TARBP and PACT. (A) Western blot showing expression and immunoprecipitation of HA-tagged TARBP2 and PACT proteins using anti-HA antibody. For TARBP2 and PACT immunoprecipitation, cultured cells were collected into 2 ml of PBS 48 h after transfection. The samples were centrifuged at maximal speed for 1 min, and the supernatants were removed. The cells were lysed with 200 μl of lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na 3 VO 4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]), passed through a needle and stored at ice for 10–15 min. The samples were centrifuged (15 min, 4°C, 12,000 g ), and the supernatants were used for immunoprecipitation. The supernatants were diluted with binding buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na 3 VO 4 , 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]) to 0.1% total concentration of IGEPAL-25. HA-probe antibody (F-7, Santa Cruz Biotech., sc-7392 AC) conjugated with agarose beads was used for the protein immunoprecipitation. The beads were added to each sample and incubated on rotator overnight at 4°C. The beads were washed with 1,000 μl of binding buffer 5 times (centrifugation at 4°C, 4,000 g , and 4 min). The last wash was placed into a new tube. Immunoprecipitated RNA was quantified using RT-qPCR. (B) Relative enrichment of CAG-EGFP-MosIR RNA upon immunoprecipitation of TARBP2 and PACT.

    Journal: Life Science Alliance

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells

    doi: 10.26508/lsa.201800289

    Figure Lengend Snippet: Expression MosIR binding by dsRBPs TARBP and PACT. (A) Western blot showing expression and immunoprecipitation of HA-tagged TARBP2 and PACT proteins using anti-HA antibody. For TARBP2 and PACT immunoprecipitation, cultured cells were collected into 2 ml of PBS 48 h after transfection. The samples were centrifuged at maximal speed for 1 min, and the supernatants were removed. The cells were lysed with 200 μl of lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na 3 VO 4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]), passed through a needle and stored at ice for 10–15 min. The samples were centrifuged (15 min, 4°C, 12,000 g ), and the supernatants were used for immunoprecipitation. The supernatants were diluted with binding buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na 3 VO 4 , 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]) to 0.1% total concentration of IGEPAL-25. HA-probe antibody (F-7, Santa Cruz Biotech., sc-7392 AC) conjugated with agarose beads was used for the protein immunoprecipitation. The beads were added to each sample and incubated on rotator overnight at 4°C. The beads were washed with 1,000 μl of binding buffer 5 times (centrifugation at 4°C, 4,000 g , and 4 min). The last wash was placed into a new tube. Immunoprecipitated RNA was quantified using RT-qPCR. (B) Relative enrichment of CAG-EGFP-MosIR RNA upon immunoprecipitation of TARBP2 and PACT.

    Article Snippet: Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]).

    Techniques: Expressing, Binding Assay, Western Blot, Immunoprecipitation, Cell Culture, Transfection, Lysis, Protease Inhibitor, Concentration Assay, Incubation, Centrifugation, Quantitative RT-PCR

    ABA stabilizes ABI5 protein. ( A ) Eight-day-old Ws/ 35S ∷ HA-ABI5 seedlings (T3 generation) transferred to MS medium with or without 50 μM ABA. Proteins were extracted at the time points indicated and ABI5 protein levels were monitored by Western blot analysis using antibodies to HA. Each lane contained 5 μg protein. ( B ) Eight-day-old Ws/ 35S ∷ HA-ABI5 seedlings were incubated in liquid MS medium supplemented with 100 μM cycloheximide for 15 h. After the treatment, 50 μM ABA was added to the medium and proteins were extracted at the times indicated. Equal amounts (5 μg) of protein were loaded on a 10% SDS/polyacrylamide (NOVEX) gel and analyzed by Western blot using an anti-HA antibody. ( C ) Extracts prepared from 8-day-old Ws/ 35S ∷ HA-ABI5 seedlings were treated with a mixture of anti-protease (complete Mini from Amersham Pharmacia) supplemented with 1 mM PMSF with or without proteasome inhibitors ALLN, MG115, MG132, and PS1 (10 μM each, Calbiochem), as described in Materials and Methods . An equal volume of SDS-loading buffer was added to stop the reactions. Equal amounts of sample were then analyzed by Western blots using rabbit antibody to HA. ( D ) Eight-day-old Ws/ 35S ∷ HA-ABI5 transgenic seedlings were treated in liquid MS medium with [ 32 P]orthophosphoric acid (100 μCi) for 1 h 30 min. Seedlings were further treated with or without 50 μM ABA for 30 min. HA-ABI5 was immunoprecipitated by using rabbit antibody to HA coupled to beads. Beads were further treated with or without γ phosphatase. 90% of the sample was then analyzed by PhosphorImager (Bio-Rad); 10% of the sample was analyzed by Western blot using mouse monoclonal antibody to HA (Santa Cruz Biotechnology) as the first antibody.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A postgermination developmental arrest checkpoint is mediated by abscisic acid and requires the ABI5 transcription factor inArabidopsis

    doi: 10.1073/pnas.081594298

    Figure Lengend Snippet: ABA stabilizes ABI5 protein. ( A ) Eight-day-old Ws/ 35S ∷ HA-ABI5 seedlings (T3 generation) transferred to MS medium with or without 50 μM ABA. Proteins were extracted at the time points indicated and ABI5 protein levels were monitored by Western blot analysis using antibodies to HA. Each lane contained 5 μg protein. ( B ) Eight-day-old Ws/ 35S ∷ HA-ABI5 seedlings were incubated in liquid MS medium supplemented with 100 μM cycloheximide for 15 h. After the treatment, 50 μM ABA was added to the medium and proteins were extracted at the times indicated. Equal amounts (5 μg) of protein were loaded on a 10% SDS/polyacrylamide (NOVEX) gel and analyzed by Western blot using an anti-HA antibody. ( C ) Extracts prepared from 8-day-old Ws/ 35S ∷ HA-ABI5 seedlings were treated with a mixture of anti-protease (complete Mini from Amersham Pharmacia) supplemented with 1 mM PMSF with or without proteasome inhibitors ALLN, MG115, MG132, and PS1 (10 μM each, Calbiochem), as described in Materials and Methods . An equal volume of SDS-loading buffer was added to stop the reactions. Equal amounts of sample were then analyzed by Western blots using rabbit antibody to HA. ( D ) Eight-day-old Ws/ 35S ∷ HA-ABI5 transgenic seedlings were treated in liquid MS medium with [ 32 P]orthophosphoric acid (100 μCi) for 1 h 30 min. Seedlings were further treated with or without 50 μM ABA for 30 min. HA-ABI5 was immunoprecipitated by using rabbit antibody to HA coupled to beads. Beads were further treated with or without γ phosphatase. 90% of the sample was then analyzed by PhosphorImager (Bio-Rad); 10% of the sample was analyzed by Western blot using mouse monoclonal antibody to HA (Santa Cruz Biotechnology) as the first antibody.

    Article Snippet: Cell debris was pelletted by centrifugation and equal amounts of extract were transferred to individual tubes with a mixture of anti-protease (complete Mini from Amersham Pharmacia) supplemented with 1 mM PMSF with or without 26S proteasome-specific inhibitors ALLN, MG115, MG132, and PS1 (10 μM each, Calbiochem).

    Techniques: Mass Spectrometry, Western Blot, Incubation, Transgenic Assay, Immunoprecipitation