protease inhibitor mix  (GE Healthcare)

 
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  • 99
    Name:
    Protease Inhibitor Mix
    Description:

    Catalog Number:
    80650123
    Price:
    None
    Category:
    Protease Inhibitor Mix
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    Structured Review

    GE Healthcare protease inhibitor mix

    https://www.bioz.com/result/protease inhibitor mix/product/GE Healthcare
    Average 99 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor mix - by Bioz Stars, 2020-11
    99/100 stars

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    Protease Inhibitor:

    Article Title: Proteomics of methyl jasmonate induced defense response in maize leaves against Asian corn borer
    Article Snippet: .. The final pellet was vacuum-dried and solubilized in 3 mL of 7 M (w/v) urea containing 2 M (w/v) thiourea, 40 mM DTT, and 1% (v/v) protease inhibitor mixture (GE Healthcare, USA) on ice for about one hour. .. Insoluble material was removed by centrifugation at 100,000 rpm for one hour.

    Article Title: Phosphorylation of Caspase-8 (Thr-263) by Ribosomal S6 Kinase 2 (RSK2) Mediates Caspase-8 Ubiquitination and Stability *
    Article Snippet: .. For immunoblotting, 30 μg of each protein sample from cells disrupted with Nonidet P-40 cell lysis buffer (50 m m Tris-Cl, pH 8.0, 150 m m NaCl, 0.5% Nonidet P-40, and protease inhibitor mixture) were used for immunoblotting with the appropriate specific primary antibodies and an alkaline phosphatase-conjugated secondary antibody and visualized using an enhanced chemifluorescence kit (Amersham Biosciences). .. For immunoprecipitation, protein samples were incubated with the appropriate antibody and agarose A/G beads (50% slurry) by rocking at 4 °C overnight.

    Article Title: The Hyr1 protein from the fungus Candida albicans is a cross kingdom immunotherapeutic target for Acinetobacter bacterial infection
    Article Snippet: .. Cells were passaged in fresh medium for 3 h (log phase) at 37°C with shaking, washed, and the resultant pellet was resuspended in disintegration buffer (7.8 g/L NaH2 PO4 , 7.1 g/L Na2 HPO4 , 0.247 g/L MgSO4 7.H2 O + protease inhibitor mix (GE Healthcare) + nuclease mix (GE Healthcare) and sonicated on ice for 3x for 5 min each with the unbroken cells separated by centrifugation at 1,500 g . ..

    Article Title: Morphological Evidence for Functional Crosstalk Between Multiple GnRH Systems in the Male Tilapia, Oreochromis niloticus
    Article Snippet: .. The brains and pituitaries were separately dissected out and homogenized in 1 ml and 100 μl of 50 mM Tris-HCl buffer (pH 7.4) containing 10 μl per ml protease inhibitor mix (GE Healthcare Life Sciences, Piscataway, NJ), respectively. .. The soluble fractions were obtained by centrifugation at 6,000 G for 30 min at 4°C.

    Article Title: PmRab7 Is a VP28-Binding Protein Involved in White Spot Syndrome Virus Infection in Shrimp
    Article Snippet: .. After ultracentrifugation, the pellet was solubilized in NaCl/phosphate buffer ( ) with 1% Triton X-100, 1× protease inhibitor mix (Amersham Biosciences). ..

    Centrifugation:

    Article Title: The Hyr1 protein from the fungus Candida albicans is a cross kingdom immunotherapeutic target for Acinetobacter bacterial infection
    Article Snippet: .. Cells were passaged in fresh medium for 3 h (log phase) at 37°C with shaking, washed, and the resultant pellet was resuspended in disintegration buffer (7.8 g/L NaH2 PO4 , 7.1 g/L Na2 HPO4 , 0.247 g/L MgSO4 7.H2 O + protease inhibitor mix (GE Healthcare) + nuclease mix (GE Healthcare) and sonicated on ice for 3x for 5 min each with the unbroken cells separated by centrifugation at 1,500 g . ..

    Lysis:

    Article Title: Phosphorylation of Caspase-8 (Thr-263) by Ribosomal S6 Kinase 2 (RSK2) Mediates Caspase-8 Ubiquitination and Stability *
    Article Snippet: .. For immunoblotting, 30 μg of each protein sample from cells disrupted with Nonidet P-40 cell lysis buffer (50 m m Tris-Cl, pH 8.0, 150 m m NaCl, 0.5% Nonidet P-40, and protease inhibitor mixture) were used for immunoblotting with the appropriate specific primary antibodies and an alkaline phosphatase-conjugated secondary antibody and visualized using an enhanced chemifluorescence kit (Amersham Biosciences). .. For immunoprecipitation, protein samples were incubated with the appropriate antibody and agarose A/G beads (50% slurry) by rocking at 4 °C overnight.

    Sonication:

    Article Title: The Hyr1 protein from the fungus Candida albicans is a cross kingdom immunotherapeutic target for Acinetobacter bacterial infection
    Article Snippet: .. Cells were passaged in fresh medium for 3 h (log phase) at 37°C with shaking, washed, and the resultant pellet was resuspended in disintegration buffer (7.8 g/L NaH2 PO4 , 7.1 g/L Na2 HPO4 , 0.247 g/L MgSO4 7.H2 O + protease inhibitor mix (GE Healthcare) + nuclease mix (GE Healthcare) and sonicated on ice for 3x for 5 min each with the unbroken cells separated by centrifugation at 1,500 g . ..

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