protease inhibitor cocktail (Roche)
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Roche
protease inhibitor cocktail
Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 8944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor cocktail/product/Roche
Average 99 stars, based on 8944 article reviews
Price from $9.99 to $1999.99
Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 8944 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor cocktail/product/Roche
Average 99 stars, based on 8944 article reviews
Price from $9.99 to $1999.99
protease inhibitor cocktail - by Bioz Stars,
2020-04
99/100 stars
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Clone Assay:Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: The amplified gene was double-digested with Nde I and Bam HI, and subsequently cloned into the expression vector pET-11a from Novagen. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Centrifugation:Article Title: Lysine Deacetylases Hda1 and Rpd3 Regulate Hsp90 Function thereby Governing Fungal Drug Resistance Article Snippet: In brief, cells were washed with sterile water and resuspended in 500 μl of lysis buffer J containing 20 mM Tris pH 7.5, 100 mM KCl, 5 mM MgCl and 20% glycerol, with one Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: Bacterial cells were pelleted by 4000g centrifugation and then re-suspended in 75mL of PBS buffer. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Article Title: Enhanced Discrimination of Malignant from Benign Pancreatic Disease by Measuring the CA 19-9 Antigen on Specific Protein Carriers Article Snippet: The sandwich assay consisted of four 1-hour-incubations in room temperature (RT) with the following reagents: 1) blocking buffer (PBS containing 0.5% Tween-20 (PBST0.5) and 1% BSA); 2) a serum or plasma sample, diluted two-fold in 1×TBS containing 0.08% Brij, 0.08 Tween-20, 50 µg/ml Amplification:Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: The amplified gene was double-digested with Nde I and Bam HI, and subsequently cloned into the expression vector pET-11a from Novagen. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Construct:Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: The resulting construct was transfected into tsA-201 cells (transfection details below). .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a Microarray:Article Title: Enhanced Discrimination of Malignant from Benign Pancreatic Disease by Measuring the CA 19-9 Antigen on Specific Protein Carriers Article Snippet: Paragraph title: Microarray assays ... The sandwich assay consisted of four 1-hour-incubations in room temperature (RT) with the following reagents: 1) blocking buffer (PBS containing 0.5% Tween-20 (PBST0.5) and 1% BSA); 2) a serum or plasma sample, diluted two-fold in 1×TBS containing 0.08% Brij, 0.08 Tween-20, 50 µg/ml Incubation:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: .. After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: Functional differences of short and long isoforms of spastin harboring missense mutation Article Snippet: Immunoblot assays, immunoprecipitation and densitometry analysis HeLa or HEK cells were lysed 18-24 h after transfection in TSE (50 mM Tris HCl pH 8.0, 150 mM NaCl, 1 mM EDTA), to which was added 1% Triton X-100 and Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Stripping Membranes:Article Title: Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling Article Snippet: 2.8 Immunoblotting Frozen, powdered tissue was homogenized by probe homogenizer in RIPA buffer containing Expressing:Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: Paragraph title: Protein expression and HBV core purification ... 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: Each fragment was ligated into the T-easy vector system (Promega), the sequence was confirmed, and the fragment was subcloned into the expression vector pCDNA3.1neo. .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Article Title: Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Article Snippet: Paragraph title: Protein Expression and Purification ... Harvested cells were resuspended in lysis buffer [50 mM KH2 PO4 (pH 6.5) and 1 mM EDTA] supplemented with Roche Western Blot:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: Paragraph title: Solubilisation, Purification and Western Blot Analysis ... After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: Cisplatin and Doxorubicin Induce Distinct Mechanisms of Ovarian Follicle Loss; Imatinib Provides Selective Protection Only against Cisplatin Article Snippet: .. Protein Extraction and Western Blotting Frozen ovaries were homogenised in 20 µl lysis buffer (containing 50 mM HEPES buffer, 10% Triton X, 50 mM NaCl, Protease inhibitor cocktail and protease inhibitors [I and II], H2 O; all purchased from Sigma Aldrich Ltd except for Transformation Assay:Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: Transformed BL21 (DE3) cells were grown in LB medium with 100µg/ml of ampicillin at 37°C overnight. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Transfection:Article Title: Functional differences of short and long isoforms of spastin harboring missense mutation Article Snippet: .. Immunoblot assays, immunoprecipitation and densitometry analysis HeLa or HEK cells were lysed 18-24 h after transfection in TSE (50 mM Tris HCl pH 8.0, 150 mM NaCl, 1 mM EDTA), to which was added 1% Triton X-100 and Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: The resulting construct was transfected into tsA-201 cells (transfection details below). .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a Immunoprecipitation:Article Title: Functional differences of short and long isoforms of spastin harboring missense mutation Article Snippet: .. Immunoblot assays, immunoprecipitation and densitometry analysis HeLa or HEK cells were lysed 18-24 h after transfection in TSE (50 mM Tris HCl pH 8.0, 150 mM NaCl, 1 mM EDTA), to which was added 1% Triton X-100 and Article Title: Lysine Deacetylases Hda1 and Rpd3 Regulate Hsp90 Function thereby Governing Fungal Drug Resistance Article Snippet: Paragraph title: Immunoprecipitation ... In brief, cells were washed with sterile water and resuspended in 500 μl of lysis buffer J containing 20 mM Tris pH 7.5, 100 mM KCl, 5 mM MgCl and 20% glycerol, with one Protease Inhibitor:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: .. After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: Functional differences of short and long isoforms of spastin harboring missense mutation Article Snippet: .. Immunoblot assays, immunoprecipitation and densitometry analysis HeLa or HEK cells were lysed 18-24 h after transfection in TSE (50 mM Tris HCl pH 8.0, 150 mM NaCl, 1 mM EDTA), to which was added 1% Triton X-100 and Article Title: Cisplatin and Doxorubicin Induce Distinct Mechanisms of Ovarian Follicle Loss; Imatinib Provides Selective Protection Only against Cisplatin Article Snippet: .. Protein Extraction and Western Blotting Frozen ovaries were homogenised in 20 µl lysis buffer (containing 50 mM HEPES buffer, 10% Triton X, 50 mM NaCl, Protease inhibitor cocktail and protease inhibitors [I and II], H2 O; all purchased from Sigma Aldrich Ltd except for Article Title: Lysine Deacetylases Hda1 and Rpd3 Regulate Hsp90 Function thereby Governing Fungal Drug Resistance Article Snippet: .. In brief, cells were washed with sterile water and resuspended in 500 μl of lysis buffer J containing 20 mM Tris pH 7.5, 100 mM KCl, 5 mM MgCl and 20% glycerol, with one Article Title: Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling Article Snippet: .. 2.8 Immunoblotting Frozen, powdered tissue was homogenized by probe homogenizer in RIPA buffer containing Article Title: Identification of Ocular Autoantigens Associated With Juvenile Idiopathic Arthritis-Associated Uveitis Article Snippet: .. The tissue specimens were then homogenized in urea lysis buffer (9 M urea, 2 M thiourea, 65 mM CHAPS, and 65 mM DTT) supplemented with 1 mM PMSF (SERVA, Heidelberg, Germany) and Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Article Title: Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Article Snippet: .. Harvested cells were resuspended in lysis buffer [50 mM KH2 PO4 (pH 6.5) and 1 mM EDTA] supplemented with Roche Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: .. Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a Article Title: Enhanced Discrimination of Malignant from Benign Pancreatic Disease by Measuring the CA 19-9 Antigen on Specific Protein Carriers Article Snippet: .. The sandwich assay consisted of four 1-hour-incubations in room temperature (RT) with the following reagents: 1) blocking buffer (PBS containing 0.5% Tween-20 (PBST0.5) and 1% BSA); 2) a serum or plasma sample, diluted two-fold in 1×TBS containing 0.08% Brij, 0.08 Tween-20, 50 µg/ml Article Title: Oesophagostomum dentatum Extract Modulates T Cell-Dependent Immune Responses to Bystander Antigens and Prevents the Development of Allergy in Mice Article Snippet: .. Bronchoalvelar Lavage and Differential Cell Counts Bronchoalveolar lavage fluid (BALF) was collected by cannulating the trachea and injecting/recovering 2×1 ml PBS containing a Cell Culture:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: Solubilisation, Purification and Western Blot Analysis Cells were grown to confluency in 6-well cell culture plates. .. After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a Protein Concentration:Article Title: Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Article Snippet: Harvested cells were resuspended in lysis buffer [50 mM KH2 PO4 (pH 6.5) and 1 mM EDTA] supplemented with Roche Polymerase Chain Reaction:Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: PCR was used to amplify (past repeat 23) and add an HA tag onto the C-terminal regions of filamins A and B. .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Binding Assay:Article Title: Enhanced Discrimination of Malignant from Benign Pancreatic Disease by Measuring the CA 19-9 Antigen on Specific Protein Carriers Article Snippet: The sandwich assay consisted of four 1-hour-incubations in room temperature (RT) with the following reagents: 1) blocking buffer (PBS containing 0.5% Tween-20 (PBST0.5) and 1% BSA); 2) a serum or plasma sample, diluted two-fold in 1×TBS containing 0.08% Brij, 0.08 Tween-20, 50 µg/ml Fluorescence:Article Title: Enhanced Discrimination of Malignant from Benign Pancreatic Disease by Measuring the CA 19-9 Antigen on Specific Protein Carriers Article Snippet: The sandwich assay consisted of four 1-hour-incubations in room temperature (RT) with the following reagents: 1) blocking buffer (PBS containing 0.5% Tween-20 (PBST0.5) and 1% BSA); 2) a serum or plasma sample, diluted two-fold in 1×TBS containing 0.08% Brij, 0.08 Tween-20, 50 µg/ml Magnetic Beads:Article Title: Functional differences of short and long isoforms of spastin harboring missense mutation Article Snippet: Immunoblot assays, immunoprecipitation and densitometry analysis HeLa or HEK cells were lysed 18-24 h after transfection in TSE (50 mM Tris HCl pH 8.0, 150 mM NaCl, 1 mM EDTA), to which was added 1% Triton X-100 and Isolation:Article Title: Identification of Ocular Autoantigens Associated With Juvenile Idiopathic Arthritis-Associated Uveitis Article Snippet: Paragraph title: Protein Isolation ... The tissue specimens were then homogenized in urea lysis buffer (9 M urea, 2 M thiourea, 65 mM CHAPS, and 65 mM DTT) supplemented with 1 mM PMSF (SERVA, Heidelberg, Germany) and Article Title: Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Article Snippet: Harvested cells were resuspended in lysis buffer [50 mM KH2 PO4 (pH 6.5) and 1 mM EDTA] supplemented with Roche Purification:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: Paragraph title: Solubilisation, Purification and Western Blot Analysis ... After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: Paragraph title: Protein expression and HBV core purification ... 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Article Title: Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Article Snippet: Paragraph title: Protein Expression and Purification ... Harvested cells were resuspended in lysis buffer [50 mM KH2 PO4 (pH 6.5) and 1 mM EDTA] supplemented with Roche Sequencing:Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: Each fragment was ligated into the T-easy vector system (Promega), the sequence was confirmed, and the fragment was subcloned into the expression vector pCDNA3.1neo. .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Protein Extraction:Article Title: Cisplatin and Doxorubicin Induce Distinct Mechanisms of Ovarian Follicle Loss; Imatinib Provides Selective Protection Only against Cisplatin Article Snippet: .. Protein Extraction and Western Blotting Frozen ovaries were homogenised in 20 µl lysis buffer (containing 50 mM HEPES buffer, 10% Triton X, 50 mM NaCl, Protease inhibitor cocktail and protease inhibitors [I and II], H2 O; all purchased from Sigma Aldrich Ltd except for Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a Blocking Assay:Article Title: Enhanced Discrimination of Malignant from Benign Pancreatic Disease by Measuring the CA 19-9 Antigen on Specific Protein Carriers Article Snippet: .. The sandwich assay consisted of four 1-hour-incubations in room temperature (RT) with the following reagents: 1) blocking buffer (PBS containing 0.5% Tween-20 (PBST0.5) and 1% BSA); 2) a serum or plasma sample, diluted two-fold in 1×TBS containing 0.08% Brij, 0.08 Tween-20, 50 µg/ml Polyacrylamide Gel Electrophoresis:Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Lysis:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: .. After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: Cisplatin and Doxorubicin Induce Distinct Mechanisms of Ovarian Follicle Loss; Imatinib Provides Selective Protection Only against Cisplatin Article Snippet: .. Protein Extraction and Western Blotting Frozen ovaries were homogenised in 20 µl lysis buffer (containing 50 mM HEPES buffer, 10% Triton X, 50 mM NaCl, Protease inhibitor cocktail and protease inhibitors [I and II], H2 O; all purchased from Sigma Aldrich Ltd except for Article Title: Lysine Deacetylases Hda1 and Rpd3 Regulate Hsp90 Function thereby Governing Fungal Drug Resistance Article Snippet: .. In brief, cells were washed with sterile water and resuspended in 500 μl of lysis buffer J containing 20 mM Tris pH 7.5, 100 mM KCl, 5 mM MgCl and 20% glycerol, with one Article Title: Identification of Ocular Autoantigens Associated With Juvenile Idiopathic Arthritis-Associated Uveitis Article Snippet: .. The tissue specimens were then homogenized in urea lysis buffer (9 M urea, 2 M thiourea, 65 mM CHAPS, and 65 mM DTT) supplemented with 1 mM PMSF (SERVA, Heidelberg, Germany) and Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Article Title: Characterization and Modeling of the Oligomeric State and Ligand Binding Behavior of Purified Translocator Protein 18 kDa from Rhodobacter sphaeroides Article Snippet: .. Harvested cells were resuspended in lysis buffer [50 mM KH2 PO4 (pH 6.5) and 1 mM EDTA] supplemented with Roche Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: .. Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a SDS Page:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: Functional differences of short and long isoforms of spastin harboring missense mutation Article Snippet: Immunoblot assays, immunoprecipitation and densitometry analysis HeLa or HEK cells were lysed 18-24 h after transfection in TSE (50 mM Tris HCl pH 8.0, 150 mM NaCl, 1 mM EDTA), to which was added 1% Triton X-100 and Article Title: Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling Article Snippet: 2.8 Immunoblotting Frozen, powdered tissue was homogenized by probe homogenizer in RIPA buffer containing Plasmid Preparation:Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: The amplified gene was double-digested with Nde I and Bam HI, and subsequently cloned into the expression vector pET-11a from Novagen. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Article Title: Modified Cav1.4 Expression in the Cacna1fnob2 Mouse Due to Alternative Splicing of an ETn Inserted in Exon 2 Article Snippet: Each fragment was ligated into the T-easy vector system (Promega), the sequence was confirmed, and the fragment was subcloned into the expression vector pCDNA3.1neo. .. After a two-day incubation the cells were lysed on ice with lysis buffer (300 mM NaCl, 50 mM Tris pH 7.5, 0.1% Triton X-100) containing a Software:Article Title: Reduced insulin action in muscle of high fat diet rats over the diurnal cycle is not associated with defective insulin signaling Article Snippet: 2.8 Immunoblotting Frozen, powdered tissue was homogenized by probe homogenizer in RIPA buffer containing Positron Emission Tomography:Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: The amplified gene was double-digested with Nde I and Bam HI, and subsequently cloned into the expression vector pET-11a from Novagen. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Homogenization:Article Title: Distinct perinatal features of the hyperpolarization-activated non-selective cation current Ih in the rat cortical plate Article Snippet: .. Tissue homogenization was performed in lysis buffer containing 20 mM Tris–HCl (pH 7.5), 0.25 M sucrose, 1 mM EGTA, and 5 mM EDTA which was supplemented with a Concentration Assay:Article Title: Ezrin is a Major Regulator of Membrane Tension in Epithelial Cells Article Snippet: After washing cells three times with PBS− , cell lysis was achieved by incubation with radioimmunoprecipitation (RIPA) buffer containing a Article Title: 3.5? cryoEM Structure of Hepatitis B Virus Core Assembled from Full-Length Core Protein Article Snippet: After IPTG was added into the culture medium with a final concentration of 0.2mM when A600 of medium reached 0.8, the bacterial cells were grown for another 4 hours to induce HBV core protein production. .. 60mg of chicken lysozyme, 15mg of DNAse1, and two tablets of Roche Staining:Article Title: Oesophagostomum dentatum Extract Modulates T Cell-Dependent Immune Responses to Bystander Antigens and Prevents the Development of Allergy in Mice Article Snippet: Bronchoalvelar Lavage and Differential Cell Counts Bronchoalveolar lavage fluid (BALF) was collected by cannulating the trachea and injecting/recovering 2×1 ml PBS containing a |
![Expression of conventional breast cancer biomarkers and selected multidrug resistance genes. Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with <t>cOmplete</t> Mini protease inhibitor and PhosSTOP phosphatase inhibitor. Quantities (10–50 μg) of total protein were run on a 10 % gel (MDR1), 4–20 % precast gels (epidermal growth factor receptor [EGFR], oestrogen receptor [ER], progesterone receptor [PR], type II topoisomerase [TOPO IIα]) and Any kD Mini-PROTEAN TGX Precast Protein Gels (human epidermal growth factor receptor 2 [HER2], HER3), transferred onto polyvinylidene fluoride membrane and developed using chemiluminescence substrate. Nat native, Epi-R epirubicin-resistant, GAPDH glyceraldehyde-3-phosphate dehydrogenase](https://storage.googleapis.com/bioz_article_images/PMC4744406/13058_2016_676_Fig2_HTML.jpg)
![ST398 CA-MRSA virulence in a mouse model. The seven ST398 CA-MRSA isolates were compared with closely related ST398 MSSA, all ST398 LA-MSSA obtained for this study, and representative Chinese ST5 and ST239 HA-MRSA strains by in vivo analysis of virulence. In addition, two USA300 CA-MRSA strains (SF8300, LAC) and the genome-sequenced LA-MRSA S0385 standard strain were used in the comparisons. Isolate selection for the MSSA and HA-MRSA groups was performed, as in a previous study [ 26 ], by selecting isolates whose average PSM production was similar to that of the entire group and thus reflects the average virulence potential. Notably, for the in vivo infection studies, the ST398 CA-MRSA, MSSA, and ST5/ST239 HA-MRSA isolates were obtained from the corresponding type of human infection (respiratory and skin infection, respectively). See Additional file 1 : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or <t>PBS</t> alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory <t>cytokine</t> expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p](https://storage.googleapis.com/bioz_article_images/PMC5789642/13073_2018_514_Fig2_HTML.jpg)
