protease inhibitor cocktail (Roche)
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protease inhibitor cocktail
Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor cocktail/product/Roche
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor cocktail/product/Roche
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
protease inhibitor cocktail - by Bioz Stars,
2021-03
86/100 stars
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Purification:Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies Article Snippet: The database also reports associated protein target characteristics critical for the purification effort including protein molecular weight with tag for the exact isotope enrichment strategy, protein isoelectric point and extinction coefficient among others. .. The centrifuge bags of targets selected for purification are thawed on ice, 30 mL of Binding Buffer (50 mM Tris-HCl, 500 mM NaCl, 40 mM Imidazole, 1 mM TCEP and 0.02% NaN3 , pH 7.5) containing a Binding Assay:Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies Article Snippet: The database also reports associated protein target characteristics critical for the purification effort including protein molecular weight with tag for the exact isotope enrichment strategy, protein isoelectric point and extinction coefficient among others. .. The centrifuge bags of targets selected for purification are thawed on ice, 30 mL of Binding Buffer (50 mM Tris-HCl, 500 mM NaCl, 40 mM Imidazole, 1 mM TCEP and 0.02% NaN3 , pH 7.5) containing a Protease Inhibitor:Article Title: Preparation of Protein Samples for NMR Structure, Function, and Small Molecule Screening Studies Article Snippet: The database also reports associated protein target characteristics critical for the purification effort including protein molecular weight with tag for the exact isotope enrichment strategy, protein isoelectric point and extinction coefficient among others. .. The centrifuge bags of targets selected for purification are thawed on ice, 30 mL of Binding Buffer (50 mM Tris-HCl, 500 mM NaCl, 40 mM Imidazole, 1 mM TCEP and 0.02% NaN3 , pH 7.5) containing a Article Title: Engineering of Bacillus thuringiensis Cry Proteins to Enhance the Activity against Western Corn Rootworm Article Snippet: The shuffled IP3 protein was diluted to 0.5 mg/mL in phosphate buffer saline containing 20% of the midgut fluid and incubated at 25 °C to observe the digestion in vitro. .. Two μL of this mixture were taken at predetermined time intervals and mixed with 20 μL of NuPAGE® LDS sample buffer (Life Technologies, Carlsbad, CA, USA) that contained a reducing agent and Roche Article Title: Computational modeling suggests dimerization of equine infectious anemia virus Rev is required for RNA binding Article Snippet: Expression and purification of EIAV Rev MBP-Rev fusion proteins were cloned and expressed in E. coli strain Rosetta Gami in NZY media as described previously [ ]. .. Following expression, cells were pelleted and resuspended in lysis buffer containing 25 mM HEPES pH 7.5, 200 mM NaCl, 2 mM beta-mercaptoethanol (BME), supplemented with 2 mM phenylmethylsulfonyl fluoride (PMSF) and Roche Article Title: Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals Article Snippet: Released HMGB1 protein content was quantified in supernatants by ELISA (#ST51011; IBL, Hamburg, Germany) according to the manufacturer’s recommendations, and samples were measured with a Paradigm E3 multilabel reader (Molecular Devices). .. Western blot Half a million cells were harvested from six-well plates resuspended in lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and Article Title: Humanizing the yeast origin recognition complex Article Snippet: In brief, WT and mutant yeast cell pellets were incubated on ice for 3 min with 50 µl of cold ATAC-RSB (10 mM Tris HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2 , 1× ROCHE Complete Protease Inhibitor Cocktail, 0.1% IGEPAL CA-630, 0.1% Tween-20, and 0.01% digitonin). .. Subsequently, 1 ml of cold ATAC-RSB with 0.1% Tween-20 (10 mM Tris HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 1× ROCHE Article Title: The proteome of cytosolic lipid droplets isolated from differentiated Caco-2/TC7 enterocytes reveals cell-specific characteristics Article Snippet: Subcellular fractionation Lipid droplets were isolated by density-gradient centrifugation from Caco-2/TC7 cells incubated for 24 h with lipid micelles, using a protocol adapted from Yu et al. ( ) and Brasaemle and Wolins ( ). .. Cell layers (two 75 mm diameter inserts per centrifuge tube) were rinsed briefly (three times with cold PBS) and scraped and the volume of the cell homogenate was adjusted to 2 ml with buffer A (25 mM Tris/HCl, pH 7.4, 100 mM KCl, 1 mM EDTA and 5 mM EGTA) containing a Article Title: Oesophagostomum dentatum Extract Modulates T Cell-Dependent Immune Responses to Bystander Antigens and Prevents the Development of Allergy in Mice Article Snippet: Mice were terminally anesthetized 72 h after the final airway challenge (day 40). .. Bronchoalvelar Lavage and Differential Cell Counts Bronchoalveolar lavage fluid (BALF) was collected by cannulating the trachea and injecting/recovering 2×1 ml PBS containing a Concentration Assay:Article Title: Engineering of Bacillus thuringiensis Cry Proteins to Enhance the Activity against Western Corn Rootworm Article Snippet: The shuffled IP3 protein was diluted to 0.5 mg/mL in phosphate buffer saline containing 20% of the midgut fluid and incubated at 25 °C to observe the digestion in vitro. .. Two μL of this mixture were taken at predetermined time intervals and mixed with 20 μL of NuPAGE® LDS sample buffer (Life Technologies, Carlsbad, CA, USA) that contained a reducing agent and Roche Expressing:Article Title: Computational modeling suggests dimerization of equine infectious anemia virus Rev is required for RNA binding Article Snippet: Expression and purification of EIAV Rev MBP-Rev fusion proteins were cloned and expressed in E. coli strain Rosetta Gami in NZY media as described previously [ ]. .. Following expression, cells were pelleted and resuspended in lysis buffer containing 25 mM HEPES pH 7.5, 200 mM NaCl, 2 mM beta-mercaptoethanol (BME), supplemented with 2 mM phenylmethylsulfonyl fluoride (PMSF) and Roche Lysis:Article Title: Computational modeling suggests dimerization of equine infectious anemia virus Rev is required for RNA binding Article Snippet: Expression and purification of EIAV Rev MBP-Rev fusion proteins were cloned and expressed in E. coli strain Rosetta Gami in NZY media as described previously [ ]. .. Following expression, cells were pelleted and resuspended in lysis buffer containing 25 mM HEPES pH 7.5, 200 mM NaCl, 2 mM beta-mercaptoethanol (BME), supplemented with 2 mM phenylmethylsulfonyl fluoride (PMSF) and Roche Article Title: Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals Article Snippet: Released HMGB1 protein content was quantified in supernatants by ELISA (#ST51011; IBL, Hamburg, Germany) according to the manufacturer’s recommendations, and samples were measured with a Paradigm E3 multilabel reader (Molecular Devices). .. Western blot Half a million cells were harvested from six-well plates resuspended in lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and Western Blot:Article Title: Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals Article Snippet: Released HMGB1 protein content was quantified in supernatants by ELISA (#ST51011; IBL, Hamburg, Germany) according to the manufacturer’s recommendations, and samples were measured with a Paradigm E3 multilabel reader (Molecular Devices). .. Western blot Half a million cells were harvested from six-well plates resuspended in lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and Incubation:Article Title: Oncolysis with DTT-205 and DTT-304 generates immunological memory in cured animals Article Snippet: Released HMGB1 protein content was quantified in supernatants by ELISA (#ST51011; IBL, Hamburg, Germany) according to the manufacturer’s recommendations, and samples were measured with a Paradigm E3 multilabel reader (Molecular Devices). .. Western blot Half a million cells were harvested from six-well plates resuspended in lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and |
![Expression of conventional breast cancer biomarkers and selected multidrug resistance genes. Cell lysates were prepared in radioimmunoprecipitation assay buffer supplemented with <t>cOmplete</t> Mini protease inhibitor and PhosSTOP phosphatase inhibitor. Quantities (10–50 μg) of total protein were run on a 10 % gel (MDR1), 4–20 % precast gels (epidermal growth factor receptor [EGFR], oestrogen receptor [ER], progesterone receptor [PR], type II topoisomerase [TOPO IIα]) and Any kD Mini-PROTEAN TGX Precast Protein Gels (human epidermal growth factor receptor 2 [HER2], HER3), transferred onto polyvinylidene fluoride membrane and developed using chemiluminescence substrate. Nat native, Epi-R epirubicin-resistant, GAPDH glyceraldehyde-3-phosphate dehydrogenase](https://storage.googleapis.com/bioz_article_images/PMC4744406/13058_2016_676_Fig2_HTML.jpg)
![ST398 CA-MRSA virulence in a mouse model. The seven ST398 CA-MRSA isolates were compared with closely related ST398 MSSA, all ST398 LA-MSSA obtained for this study, and representative Chinese ST5 and ST239 HA-MRSA strains by in vivo analysis of virulence. In addition, two USA300 CA-MRSA strains (SF8300, LAC) and the genome-sequenced LA-MRSA S0385 standard strain were used in the comparisons. Isolate selection for the MSSA and HA-MRSA groups was performed, as in a previous study [ 26 ], by selecting isolates whose average PSM production was similar to that of the entire group and thus reflects the average virulence potential. Notably, for the in vivo infection studies, the ST398 CA-MRSA, MSSA, and ST5/ST239 HA-MRSA isolates were obtained from the corresponding type of human infection (respiratory and skin infection, respectively). See Additional file 1 : Table S2 for isolates that were compared and their characteristics. a Mouse skin infection model. Mice received 10 7 live S. aureus or <t>PBS</t> alone in the right flank by subcutaneous injection. Skin abscesses (length × width) were measured 48 h after infection. b Mouse lung infection model. We pipetted 4 × 10 9 CFU/40 μl S. aureus into the nares of the anesthetized mice. All mice were euthanized 48 h after inoculation and CFU in the lung tissue were determined. a , b Three mice were infected with each strain; the shown symbols represent the average value obtained from three mice. c – f Inflammatory <t>cytokine</t> expression in ST398 CA-MRSA isolates in comparison. The inflammatory cytokines TNF-α and IL-6 were determined in skin ( c , d ) or lung tissue ( e , f ). a – f Statistical analysis is by one-way ANOVA with Dunnett’s post test versus the ST398 CA-MRSA group. * p](https://storage.googleapis.com/bioz_article_images/PMC5789642/13073_2018_514_Fig2_HTML.jpg)
