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Boehringer Mannheim protease inhibitor cocktail
Protease Inhibitor Cocktail, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protease inhibitor cocktail/product/Boehringer Mannheim
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
protease inhibitor cocktail - by Bioz Stars, 2021-03
86/100 stars

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Protease Inhibitor:

Article Title: NLRP3-inflammasome inhibition prevents high fat and high sugar diets-induced heart damage through autophagy induction
Article Snippet: Finally, anti-IL-1β (p17), anti-OGG-1, anti-Bcl-2, anti-Bax, anti-MnSOD, anti-catalase, anti-ATG12 and anti-MAP-LC3 antibodies from (Santa Cruz Biotechnology). .. A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim (Indianapolis, IN). .. The Immun Star HRP substrate kit was obtained from Bio-Rad Laboratories Inc. (Hercules, CA).

Article Title: NLRP3 inflammasome suppression improves longevity and prevents cardiac aging in male mice, et al. NLRP3 inflammasome suppression improves longevity and prevents cardiac aging in male mice
Article Snippet: Finally, anti‐Bcl‐2, anti‐Bax, anti‐ATG12, and anti‐MAP‐LC3 antibodies were obtained from Santa Cruz Biotechnology. .. A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim. .. The Immun Star HRP substrate kit was obtained from Bio‐Rad Laboratories Inc.

Article Title: Blockade of the NLRP3 inflammasome improves metabolic health and lifespan in obese mice
Article Snippet: Finally, anti-Bcl-2, anti-Bax, and anti-MAP-LC3 antibodies from (Santa Cruz Biotechnology). .. A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim (Indianapolis, IN). .. The Immun Star HRP substrate kit was obtained from Bio-Rad Laboratories Inc. (Hercules, CA).

Article Title: Butyrate Induced Cell Cycle Arrest in Bovine Cells through Targeting Gene Expression Relevant to DNA Replication Apparatus 1
Article Snippet: .. Cells were extracted with 10 volumes of M-PER (Mammalian Protein Extraction Reagent, Pierce Biotechnology, Rockford, IL) supplemented with 150 mM NaCl and protease inhibitor cocktail (Protease inhibitor cocktail tablets, Boehringer Mannheim, GmbH, Germany) and then incubated on ice for 10 min before centrifugation (1500 g, 5 min at 4 ° in Eppendorf microfuge) to eliminate debris. .. SDS-PAGE was done in a 4 to 20% gradient polyacrylamide gel (Invitrogen) under the reducing conditions suggested by the manufacturer.

Article Title: Mucosal Vaccination against Serogroup B Meningococci: Induction of Bactericidal Antibodies and Cellular Immunity following Intranasal Immunization with NadA of Neisseria meningitidis and Mutants of Escherichia coli Heat-Labile Enterotoxin
Article Snippet: .. Nasal lavage was carried out by flushing the nares three times with a total volume of 0.5 ml of PBS containing 0.5% bovine serum albumin (BSA) and a protease inhibitor cocktail (Complete TM protease inhibitor cocktail; Boehringer Mannheim, Mannheim, Germany). .. Lungs were removed and homogenized in RPMI medium containing 8% fetal calf serum (Sigma, Poole, United Kingdom) and the protease inhibitor phenylmethylsulfonyl fluoride (0.1 mM; Sigma).

Article Title: Inhibition of the NLRP3 inflammasome prevents ovarian aging
Article Snippet: NLRP3 inhibitors MCC950 and 16673-34-0 were obtained from Sigma-Aldrich (Saint Louis, USA) and R & D Systems (Minneapolis, USA), respectively. .. A cocktail of protease inhibitors (cOmplete Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim (Indianapolis, IN). .. The Immun-Star horseradish peroxidase (HRP) substrate kit was obtained from Bio-Rad Laboratories Inc. (Hercules, CA).

Article Title: Inhibition of the NLRP3 inflammasome prevents ovarian aging
Article Snippet: NLRP3 inhibitors MCC950 and 16673-34-0 were obtained from Sigma-Aldrich (Saint Louis, USA) and R & D Systems (Minneapolis, USA) respectively. .. A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim (Indianapolis, IN). .. The Immun Star HRP substrate kit was obtained from Bio-Rad Laboratories Inc. (Hercules, CA).

Protein Extraction:

Article Title: Butyrate Induced Cell Cycle Arrest in Bovine Cells through Targeting Gene Expression Relevant to DNA Replication Apparatus 1
Article Snippet: .. Cells were extracted with 10 volumes of M-PER (Mammalian Protein Extraction Reagent, Pierce Biotechnology, Rockford, IL) supplemented with 150 mM NaCl and protease inhibitor cocktail (Protease inhibitor cocktail tablets, Boehringer Mannheim, GmbH, Germany) and then incubated on ice for 10 min before centrifugation (1500 g, 5 min at 4 ° in Eppendorf microfuge) to eliminate debris. .. SDS-PAGE was done in a 4 to 20% gradient polyacrylamide gel (Invitrogen) under the reducing conditions suggested by the manufacturer.

Incubation:

Article Title: Butyrate Induced Cell Cycle Arrest in Bovine Cells through Targeting Gene Expression Relevant to DNA Replication Apparatus 1
Article Snippet: .. Cells were extracted with 10 volumes of M-PER (Mammalian Protein Extraction Reagent, Pierce Biotechnology, Rockford, IL) supplemented with 150 mM NaCl and protease inhibitor cocktail (Protease inhibitor cocktail tablets, Boehringer Mannheim, GmbH, Germany) and then incubated on ice for 10 min before centrifugation (1500 g, 5 min at 4 ° in Eppendorf microfuge) to eliminate debris. .. SDS-PAGE was done in a 4 to 20% gradient polyacrylamide gel (Invitrogen) under the reducing conditions suggested by the manufacturer.

Centrifugation:

Article Title: Butyrate Induced Cell Cycle Arrest in Bovine Cells through Targeting Gene Expression Relevant to DNA Replication Apparatus 1
Article Snippet: .. Cells were extracted with 10 volumes of M-PER (Mammalian Protein Extraction Reagent, Pierce Biotechnology, Rockford, IL) supplemented with 150 mM NaCl and protease inhibitor cocktail (Protease inhibitor cocktail tablets, Boehringer Mannheim, GmbH, Germany) and then incubated on ice for 10 min before centrifugation (1500 g, 5 min at 4 ° in Eppendorf microfuge) to eliminate debris. .. SDS-PAGE was done in a 4 to 20% gradient polyacrylamide gel (Invitrogen) under the reducing conditions suggested by the manufacturer.

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    Boehringer Mannheim immunoprecipitation ip buffer
    Full-length BCL-6 and BCoR interact in vitro and in vivo, the POZ domain of BCL-6 is both necessary and sufficient for interaction with BCoR in vitro, and BCL-6 and BCoR colocalize in the nucleus. ( A ) Structure of the proteins used in B and C . (Solid oval) The POZ domain; (solid rectangle) zinc fingers. (*) The myc epitope tag. ( B ) Coimmunoprecipitation of BCL-6 and BCoR in vitro. Pair-wise combinations of [ 35 S]methionine-labeled nontagged BCL-6 derivatives and myc-tagged BCoR were produced by cotranslation and immunoprecipitated with the α-myc antibody. The immune complexes were recovered and analyzed by SDS-PAGE (10% for lanes 1–5; 17% for lanes 6,7 ). The intensity of myc–BCoR in lanes 6 and 7 appears stronger due to compression on the 17% gel of non-full-length myc-BCoR proteins. Input represents 20% of the total used. (●) The expected migration of the nontagged BCL-6 derivatives. ( C ) Coimmunoprecipitation of BCL-6 and BCoR in vivo. 293 cells were transfected with 3 μg of BCL-6 expression plasmid alone (lane 2 ) or cotransfected with 3 μg each of BCL-6 and myc-tagged BCoR expression plasmids (lanes 1,3 ). Cell lysates were immunoprecipitated with α-myc antibody (lanes 2,3 ) and the recovered proteins were detected by Western blot analysis using N-3 α-BCL-6 and α-myc antibodies. The input (lane 1 ) corresponds to 1/50 of the lysate used in the <t>immunoprecipitation</t> (lane 3 ). ( D ) Subcellular localization of BCoR and BCoR-S. Immunofluorescence was performed on HeLa cells transfected with 1.2 μg of either myc-tagged BCoR or BCoR-S expression plasmids. Multiple Z series confocal microscopy images of the given fields were compiled for each image of BCoR and BCoR-S. ( E ) Colocalization of BCL-6 and BCoR. Coimmunofluorescence was performed on HeLa cells cotransfected with 0.6 μg each of BCL-6 and myc-tagged BCoR, and the data collected as in D . The compiled images of BCoR and BCL-6 were merged to detect colocalization.
    Immunoprecipitation Ip Buffer, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunoprecipitation ip buffer/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunoprecipitation ip buffer - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    86
    Boehringer Mannheim protease inhibitor cocktail
    Full-length BCL-6 and BCoR interact in vitro and in vivo, the POZ domain of BCL-6 is both necessary and sufficient for interaction with BCoR in vitro, and BCL-6 and BCoR colocalize in the nucleus. ( A ) Structure of the proteins used in B and C . (Solid oval) The POZ domain; (solid rectangle) zinc fingers. (*) The myc epitope tag. ( B ) Coimmunoprecipitation of BCL-6 and BCoR in vitro. Pair-wise combinations of [ 35 S]methionine-labeled nontagged BCL-6 derivatives and myc-tagged BCoR were produced by cotranslation and immunoprecipitated with the α-myc antibody. The immune complexes were recovered and analyzed by SDS-PAGE (10% for lanes 1–5; 17% for lanes 6,7 ). The intensity of myc–BCoR in lanes 6 and 7 appears stronger due to compression on the 17% gel of non-full-length myc-BCoR proteins. Input represents 20% of the total used. (●) The expected migration of the nontagged BCL-6 derivatives. ( C ) Coimmunoprecipitation of BCL-6 and BCoR in vivo. 293 cells were transfected with 3 μg of BCL-6 expression plasmid alone (lane 2 ) or cotransfected with 3 μg each of BCL-6 and myc-tagged BCoR expression plasmids (lanes 1,3 ). Cell lysates were immunoprecipitated with α-myc antibody (lanes 2,3 ) and the recovered proteins were detected by Western blot analysis using N-3 α-BCL-6 and α-myc antibodies. The input (lane 1 ) corresponds to 1/50 of the lysate used in the <t>immunoprecipitation</t> (lane 3 ). ( D ) Subcellular localization of BCoR and BCoR-S. Immunofluorescence was performed on HeLa cells transfected with 1.2 μg of either myc-tagged BCoR or BCoR-S expression plasmids. Multiple Z series confocal microscopy images of the given fields were compiled for each image of BCoR and BCoR-S. ( E ) Colocalization of BCL-6 and BCoR. Coimmunofluorescence was performed on HeLa cells cotransfected with 0.6 μg each of BCL-6 and myc-tagged BCoR, and the data collected as in D . The compiled images of BCoR and BCL-6 were merged to detect colocalization.
    Protease Inhibitor Cocktail, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail - by Bioz Stars, 2021-03
    86/100 stars
      Buy from Supplier

    Image Search Results


    Full-length BCL-6 and BCoR interact in vitro and in vivo, the POZ domain of BCL-6 is both necessary and sufficient for interaction with BCoR in vitro, and BCL-6 and BCoR colocalize in the nucleus. ( A ) Structure of the proteins used in B and C . (Solid oval) The POZ domain; (solid rectangle) zinc fingers. (*) The myc epitope tag. ( B ) Coimmunoprecipitation of BCL-6 and BCoR in vitro. Pair-wise combinations of [ 35 S]methionine-labeled nontagged BCL-6 derivatives and myc-tagged BCoR were produced by cotranslation and immunoprecipitated with the α-myc antibody. The immune complexes were recovered and analyzed by SDS-PAGE (10% for lanes 1–5; 17% for lanes 6,7 ). The intensity of myc–BCoR in lanes 6 and 7 appears stronger due to compression on the 17% gel of non-full-length myc-BCoR proteins. Input represents 20% of the total used. (●) The expected migration of the nontagged BCL-6 derivatives. ( C ) Coimmunoprecipitation of BCL-6 and BCoR in vivo. 293 cells were transfected with 3 μg of BCL-6 expression plasmid alone (lane 2 ) or cotransfected with 3 μg each of BCL-6 and myc-tagged BCoR expression plasmids (lanes 1,3 ). Cell lysates were immunoprecipitated with α-myc antibody (lanes 2,3 ) and the recovered proteins were detected by Western blot analysis using N-3 α-BCL-6 and α-myc antibodies. The input (lane 1 ) corresponds to 1/50 of the lysate used in the immunoprecipitation (lane 3 ). ( D ) Subcellular localization of BCoR and BCoR-S. Immunofluorescence was performed on HeLa cells transfected with 1.2 μg of either myc-tagged BCoR or BCoR-S expression plasmids. Multiple Z series confocal microscopy images of the given fields were compiled for each image of BCoR and BCoR-S. ( E ) Colocalization of BCL-6 and BCoR. Coimmunofluorescence was performed on HeLa cells cotransfected with 0.6 μg each of BCL-6 and myc-tagged BCoR, and the data collected as in D . The compiled images of BCoR and BCL-6 were merged to detect colocalization.

    Journal: Genes & Development

    Article Title: BCoR, a novel corepressor involved in BCL-6 repression

    doi:

    Figure Lengend Snippet: Full-length BCL-6 and BCoR interact in vitro and in vivo, the POZ domain of BCL-6 is both necessary and sufficient for interaction with BCoR in vitro, and BCL-6 and BCoR colocalize in the nucleus. ( A ) Structure of the proteins used in B and C . (Solid oval) The POZ domain; (solid rectangle) zinc fingers. (*) The myc epitope tag. ( B ) Coimmunoprecipitation of BCL-6 and BCoR in vitro. Pair-wise combinations of [ 35 S]methionine-labeled nontagged BCL-6 derivatives and myc-tagged BCoR were produced by cotranslation and immunoprecipitated with the α-myc antibody. The immune complexes were recovered and analyzed by SDS-PAGE (10% for lanes 1–5; 17% for lanes 6,7 ). The intensity of myc–BCoR in lanes 6 and 7 appears stronger due to compression on the 17% gel of non-full-length myc-BCoR proteins. Input represents 20% of the total used. (●) The expected migration of the nontagged BCL-6 derivatives. ( C ) Coimmunoprecipitation of BCL-6 and BCoR in vivo. 293 cells were transfected with 3 μg of BCL-6 expression plasmid alone (lane 2 ) or cotransfected with 3 μg each of BCL-6 and myc-tagged BCoR expression plasmids (lanes 1,3 ). Cell lysates were immunoprecipitated with α-myc antibody (lanes 2,3 ) and the recovered proteins were detected by Western blot analysis using N-3 α-BCL-6 and α-myc antibodies. The input (lane 1 ) corresponds to 1/50 of the lysate used in the immunoprecipitation (lane 3 ). ( D ) Subcellular localization of BCoR and BCoR-S. Immunofluorescence was performed on HeLa cells transfected with 1.2 μg of either myc-tagged BCoR or BCoR-S expression plasmids. Multiple Z series confocal microscopy images of the given fields were compiled for each image of BCoR and BCoR-S. ( E ) Colocalization of BCL-6 and BCoR. Coimmunofluorescence was performed on HeLa cells cotransfected with 0.6 μg each of BCL-6 and myc-tagged BCoR, and the data collected as in D . The compiled images of BCoR and BCL-6 were merged to detect colocalization.

    Article Snippet: Transfected cells were washed twice with PBS and lysed in 500 μl of cold immunoprecipitation (IP) buffer (PBS, 10% glycerol, 0.5% NP-40, and a complete protease inhibitor cocktail from Boehringer-Mannheim) for 10 min on ice.

    Techniques: In Vitro, In Vivo, Zinc-Fingers, Labeling, Produced, Immunoprecipitation, SDS Page, Migration, Transfection, Expressing, Plasmid Preparation, Western Blot, Immunofluorescence, Confocal Microscopy