protease inhibitor cocktail roche applied science  (Roche)

 
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    Structured Review

    Roche protease inhibitor cocktail roche applied science
    Protease Inhibitor Cocktail Roche Applied Science, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitor cocktail roche applied science/product/Roche
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    protease inhibitor cocktail roche applied science - by Bioz Stars, 2020-04
    95/100 stars

    Related Products / Commonly Used Together

    triton x‐100
    whole-cell lysates

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    Related Articles

    Centrifugation:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA. .. After centrifugation at 20 000 × g , 4°C, supernatant was incubated with 0.05 mg/mL of anti‐Tom40 antibodies for 3 hours 4°C followed by addition of protein A sepharose (GE Healthcare, Uppsala, Sweden) and incubation for 1 hour at 4°C.

    Protease Inhibitor:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: .. Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA. .. After centrifugation at 20 000 × g , 4°C, supernatant was incubated with 0.05 mg/mL of anti‐Tom40 antibodies for 3 hours 4°C followed by addition of protein A sepharose (GE Healthcare, Uppsala, Sweden) and incubation for 1 hour at 4°C.

    Article Title: The translational machinery of human CD4+ T cells is poised for activation and controls the switch from quiescence to metabolic remodelling
    Article Snippet: .. Whole-cell lysates were prepared using RIPA buffer (10 mM Tris-HCl, pH 7.4, 1% sodium deoxycholate, 1% TritonX-100, 0.1% SDS, 150 mM NaCl and 1 mM EDTA, pH 8.0) supplemented with complete Protease Inhibitor Cocktail Roche Applied Science). .. Cell lysates were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Merck Millipore).

    Cell Culture:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Paragraph title: 2.7. Cell culture, cell fractionation and coimmunoprecipitation experiments ... Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA.

    Immunoprecipitation:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Immunoprecipitation was performed from SH‐SY5Y cells (0.2 mg of protein) treated with DMSO or 50 μmol/L of GMP‐1 for 12 hours. .. Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA.

    Incubation:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA. .. After centrifugation at 20 000 × g , 4°C, supernatant was incubated with 0.05 mg/mL of anti‐Tom40 antibodies for 3 hours 4°C followed by addition of protein A sepharose (GE Healthcare, Uppsala, Sweden) and incubation for 1 hour at 4°C.

    Expressing:

    Article Title: The translational machinery of human CD4+ T cells is poised for activation and controls the switch from quiescence to metabolic remodelling
    Article Snippet: Whole-cell lysates were prepared using RIPA buffer (10 mM Tris-HCl, pH 7.4, 1% sodium deoxycholate, 1% TritonX-100, 0.1% SDS, 150 mM NaCl and 1 mM EDTA, pH 8.0) supplemented with complete Protease Inhibitor Cocktail Roche Applied Science). .. For ACC1 protein expression analysis, cells were treated with Rapamycin (100 nM), added at the onset of the cultures, 100 μg/ml cycloheximide or 5μg/ml actinomycin D for 24h after 24 h of Dynabeads and with 10 μM 4EGi-1 for 48 h or 24 h after 24 h or 48 h of Dynabeads stimulation, respectively.

    Cell Fractionation:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Paragraph title: 2.7. Cell culture, cell fractionation and coimmunoprecipitation experiments ... Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA.

    Western Blot:

    Article Title: The translational machinery of human CD4+ T cells is poised for activation and controls the switch from quiescence to metabolic remodelling
    Article Snippet: Paragraph title: Western blotting ... Whole-cell lysates were prepared using RIPA buffer (10 mM Tris-HCl, pH 7.4, 1% sodium deoxycholate, 1% TritonX-100, 0.1% SDS, 150 mM NaCl and 1 mM EDTA, pH 8.0) supplemented with complete Protease Inhibitor Cocktail Roche Applied Science).

    SDS Page:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA. .. Proteins were resolved by SDS‐PAGE, transferred to the nitrocellulose membrane and stained with respective antibodies.

    Article Title: The translational machinery of human CD4+ T cells is poised for activation and controls the switch from quiescence to metabolic remodelling
    Article Snippet: Whole-cell lysates were prepared using RIPA buffer (10 mM Tris-HCl, pH 7.4, 1% sodium deoxycholate, 1% TritonX-100, 0.1% SDS, 150 mM NaCl and 1 mM EDTA, pH 8.0) supplemented with complete Protease Inhibitor Cocktail Roche Applied Science). .. Cell lysates were separated by SDS-PAGE and transferred to polyvinylidene fluoride membranes (Merck Millipore).

    Staining:

    Article Title: Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease. Development of GMP‐1 a molecular chaperone network modulator protecting mitochondrial function and its assessment in fly and mice models of Alzheimer's disease
    Article Snippet: Cells were scraped from the surface in the presence of TBS with 0.2% Triton X‐100 and protease inhibitor cocktail Roche Applied Science, Indianapolis, IN, USA. .. Proteins were resolved by SDS‐PAGE, transferred to the nitrocellulose membrane and stained with respective antibodies.

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    Roche complete mini edta free protease inhibitor cocktail
    Apocatalase polypeptide proteolysis is induced in late exponential growth phase E. faecalis cells. A , lysates from strain OG1RF cells grown in heme-free medium until early exponential growth phase ( sample A ) or late exponential growth phase ( sample B ) and lysates from cells grown in heme-supplemented medium until late exponential growth phase ( sample C ) were analyzed individually or as mixtures as indicated. The samples were incubated at 37 °C, and the presence of KatA polypeptide in aliquots removed at the indicated time points was determined by immuno-blot. B , proteolysis of KatA in sample A mixed with sample B in the presence of 1 m m <t>PMSF</t> and/or <t>EDTA</t> or Complete EDTA-free protease inhibitor cocktail. The reference, sample A + B without any additions, is indicated by Ctrl .
    Complete Mini Edta Free Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete mini edta free protease inhibitor cocktail/product/Roche
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    complete mini edta free protease inhibitor cocktail - by Bioz Stars, 2020-04
    99/100 stars
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    80
    Roche 1ãƒâ— complete protease inhibitor cocktail
    Subtilisin digestion of HIV-1 indicates Nullbasic is packaged in virions. HIV-1 virus stocks described used for velocity gradient analysis were pelleted through a 20% sucrose cushion. Samples of each concentrated virus were incubated with or without <t>1×</t>
    1ãƒâ— Complete Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1ãƒâ— complete protease inhibitor cocktail/product/Roche
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1ãƒâ— complete protease inhibitor cocktail - by Bioz Stars, 2020-04
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    93
    Roche anti proteases
    Subtilisin digestion of HIV-1 indicates Nullbasic is packaged in virions. HIV-1 virus stocks described used for velocity gradient analysis were pelleted through a 20% sucrose cushion. Samples of each concentrated virus were incubated with or without <t>1×</t>
    Anti Proteases, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti proteases/product/Roche
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti proteases - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    99
    Roche protease inhibitors
    Subtilisin digestion of HIV-1 indicates Nullbasic is packaged in virions. HIV-1 virus stocks described used for velocity gradient analysis were pelleted through a 20% sucrose cushion. Samples of each concentrated virus were incubated with or without <t>1×</t>
    Protease Inhibitors, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 6503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/protease inhibitors/product/Roche
    Average 99 stars, based on 6503 article reviews
    Price from $9.99 to $1999.99
    protease inhibitors - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    Image Search Results


    Apocatalase polypeptide proteolysis is induced in late exponential growth phase E. faecalis cells. A , lysates from strain OG1RF cells grown in heme-free medium until early exponential growth phase ( sample A ) or late exponential growth phase ( sample B ) and lysates from cells grown in heme-supplemented medium until late exponential growth phase ( sample C ) were analyzed individually or as mixtures as indicated. The samples were incubated at 37 °C, and the presence of KatA polypeptide in aliquots removed at the indicated time points was determined by immuno-blot. B , proteolysis of KatA in sample A mixed with sample B in the presence of 1 m m PMSF and/or EDTA or Complete EDTA-free protease inhibitor cocktail. The reference, sample A + B without any additions, is indicated by Ctrl .

    Journal: The Journal of Biological Chemistry

    Article Title: In Vitro Assembly of Catalase *

    doi: 10.1074/jbc.M114.596148

    Figure Lengend Snippet: Apocatalase polypeptide proteolysis is induced in late exponential growth phase E. faecalis cells. A , lysates from strain OG1RF cells grown in heme-free medium until early exponential growth phase ( sample A ) or late exponential growth phase ( sample B ) and lysates from cells grown in heme-supplemented medium until late exponential growth phase ( sample C ) were analyzed individually or as mixtures as indicated. The samples were incubated at 37 °C, and the presence of KatA polypeptide in aliquots removed at the indicated time points was determined by immuno-blot. B , proteolysis of KatA in sample A mixed with sample B in the presence of 1 m m PMSF and/or EDTA or Complete EDTA-free protease inhibitor cocktail. The reference, sample A + B without any additions, is indicated by Ctrl .

    Article Snippet: To test protection of KatA by protease inhibitors 1 mm PMSF and/or 1 mm EDTA, or 1× Complete mini EDTA-free protease inhibitor cocktail (Roche Applied Science), were added to lysate from late exponential growth phase cells.

    Techniques: Incubation, Protease Inhibitor

    Subtilisin digestion of HIV-1 indicates Nullbasic is packaged in virions. HIV-1 virus stocks described used for velocity gradient analysis were pelleted through a 20% sucrose cushion. Samples of each concentrated virus were incubated with or without 1×

    Journal: Journal of Virology

    Article Title: A Mutant Tat Protein Inhibits HIV-1 Reverse Transcription by Targeting the Reverse Transcription Complex

    doi: 10.1128/JVI.03440-14

    Figure Lengend Snippet: Subtilisin digestion of HIV-1 indicates Nullbasic is packaged in virions. HIV-1 virus stocks described used for velocity gradient analysis were pelleted through a 20% sucrose cushion. Samples of each concentrated virus were incubated with or without 1×

    Article Snippet: The cells were lysed at 48 h posttransfection in S100 buffer (10 mM Tris [pH 7.4], 1.5 mM MgCl2 , 10 mM KCl, 1× complete protease inhibitor cocktail [Roche Applied Science], 0.5 mM β-mercaptoethanol) using a Dounce homogenizer.

    Techniques: Incubation