propidium iodide pi  (Thermo Fisher)


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    Name:
    Propidium Iodide
    Description:
    Propidium iodide PI is a popular red fluorescent nuclear and chromosome counterstain Since propidium iodide is not permeant to live cells it is also commonly used to detect dead cells in a population PI binds to DNA by intercalating between the bases with little or no sequence preference In aqueous solution the dye has excitation emission maxima of 493 636 nm Once the dye is bound its fluorescence is enhanced 20 to 30 fold the fluorescence excitation maximum is shifted 30 40 nm to the red and the fluorescence emission maximum is shifted 15 nm to the blue resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm PI is widely used in fluorescence microscopy confocal laser scanning microscopy flow cytometry and fluorometry Learn more about propidium iodide and propidium iodide containing products
    Catalog Number:
    P1304MP
    Price:
    None
    Category:
    Labeling Detection Products
    Applications:
    Cell Analysis|Cell Viability & Cytotoxicity|Cellular Imaging|Chromatin-Related Products|Flow Cytometry Controls, Standards & General Reagents|Flow Cytometry Staining by Cell Structure|Flow Cytometry Viability & Cytotoxicity Assays|Flow Cytometry of Cellular Processes|Immunofluorescence (IF)|Immunofluorescence Counterstaining, Mounting & Fade Prevention|Immunofluorescence Staining & Detection|Nuclear Stains|Nucleus, Nucleoli & Nuclear Envelope|Organelle Tracing|RNAi, Epigenetics & Non-Coding RNA Research|Chromatin Biology|Flow Cytometry|Cell Tracing & Tracking|Cell Structure|Cell Viability, Proliferation & Function
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    Structured Review

    Thermo Fisher propidium iodide pi
    Whole-mount clearing of the marine crab, Philyra sp. a Specimen before (top) and after (bottom) clearing. Dark parts seen through the carapace are the gut content. Grid = 5 mm. b , c Nuclear staining with <t>propidium</t> iodide (PI). Dorsal view through the carapace and the right cheliped. Scale bars = 1 mm
    Propidium iodide PI is a popular red fluorescent nuclear and chromosome counterstain Since propidium iodide is not permeant to live cells it is also commonly used to detect dead cells in a population PI binds to DNA by intercalating between the bases with little or no sequence preference In aqueous solution the dye has excitation emission maxima of 493 636 nm Once the dye is bound its fluorescence is enhanced 20 to 30 fold the fluorescence excitation maximum is shifted 30 40 nm to the red and the fluorescence emission maximum is shifted 15 nm to the blue resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm PI is widely used in fluorescence microscopy confocal laser scanning microscopy flow cytometry and fluorometry Learn more about propidium iodide and propidium iodide containing products
    https://www.bioz.com/result/propidium iodide pi/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    propidium iodide pi - by Bioz Stars, 2021-04
    99/100 stars

    Images

    1) Product Images from "Aqueous-based tissue clearing in crustaceans"

    Article Title: Aqueous-based tissue clearing in crustaceans

    Journal: Zoological Letters

    doi: 10.1186/s40851-018-0099-6

    Whole-mount clearing of the marine crab, Philyra sp. a Specimen before (top) and after (bottom) clearing. Dark parts seen through the carapace are the gut content. Grid = 5 mm. b , c Nuclear staining with propidium iodide (PI). Dorsal view through the carapace and the right cheliped. Scale bars = 1 mm
    Figure Legend Snippet: Whole-mount clearing of the marine crab, Philyra sp. a Specimen before (top) and after (bottom) clearing. Dark parts seen through the carapace are the gut content. Grid = 5 mm. b , c Nuclear staining with propidium iodide (PI). Dorsal view through the carapace and the right cheliped. Scale bars = 1 mm

    Techniques Used: Staining

    Propidium iodide (PI) staining of a cleared A. vulgare male. a Top view of the stained specimen. Dotted lines indicate the contour of male reproductive organs. Legs are removed. Scale bar = 2 mm. b Side view of the middle body part. Scale bar = 1 mm. Testis follicles (Tf), sperm vesicle (Sv), vas deferens (Vd). c Close-up image of the dorsal anterior hindgut. Large cell nuclei aligned in ordered rows. Scale bar = 500 μm
    Figure Legend Snippet: Propidium iodide (PI) staining of a cleared A. vulgare male. a Top view of the stained specimen. Dotted lines indicate the contour of male reproductive organs. Legs are removed. Scale bar = 2 mm. b Side view of the middle body part. Scale bar = 1 mm. Testis follicles (Tf), sperm vesicle (Sv), vas deferens (Vd). c Close-up image of the dorsal anterior hindgut. Large cell nuclei aligned in ordered rows. Scale bar = 500 μm

    Techniques Used: Staining

    2) Product Images from "Phagocytic response of astrocytes to damaged neighboring cells"

    Article Title: Phagocytic response of astrocytes to damaged neighboring cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0196153

    Propidium iodide stained nuclear material is incorporated into endocytic vesicles of responding astrocytes. Prior to laser exposure, PI was added to the medium surrounding primary astrocytes. Minimal intracellular PI fluorescence was observed within living cells, as visualized in the “Pre Laser-Irradiation, PI Fluorescence” images. After laser irradiation, PI is incorporated into the exposed DNA of the targeted cell along the yellow ROI, and was visualized as an increase in fluorescence intensity in “Post Laser-Irradiation” images (yellow arrow heads). Fluorescence of the exposed nuclear material continues to increase, producing a bright signal in the region of the targeted nucleus. This was visible 47 minutes post fluorescence in the first example and 188 minutes in the second example. Large endocytic vesicles were observed within the responding astrocytes (white asterisk) 47 and 188 minutes following laser-irradiation. Overlay images of phase and fluorescence confirm that the PI-stained nuclear material was located within the large endocytic vesicles (white arrows).
    Figure Legend Snippet: Propidium iodide stained nuclear material is incorporated into endocytic vesicles of responding astrocytes. Prior to laser exposure, PI was added to the medium surrounding primary astrocytes. Minimal intracellular PI fluorescence was observed within living cells, as visualized in the “Pre Laser-Irradiation, PI Fluorescence” images. After laser irradiation, PI is incorporated into the exposed DNA of the targeted cell along the yellow ROI, and was visualized as an increase in fluorescence intensity in “Post Laser-Irradiation” images (yellow arrow heads). Fluorescence of the exposed nuclear material continues to increase, producing a bright signal in the region of the targeted nucleus. This was visible 47 minutes post fluorescence in the first example and 188 minutes in the second example. Large endocytic vesicles were observed within the responding astrocytes (white asterisk) 47 and 188 minutes following laser-irradiation. Overlay images of phase and fluorescence confirm that the PI-stained nuclear material was located within the large endocytic vesicles (white arrows).

    Techniques Used: Staining, Fluorescence, Irradiation

    3) Product Images from "Givinostat, a type II histone deacetylase inhibitor, induces potent caspase-dependent apoptosis in human lymphoblastic leukemia"

    Article Title: Givinostat, a type II histone deacetylase inhibitor, induces potent caspase-dependent apoptosis in human lymphoblastic leukemia

    Journal: Genes & Cancer

    doi: 10.18632/genesandcancer.117

    Induction of apoptosis by Givinostat on leukemia cell lines The induction of apoptosis measured by sub-G0/G1 fractions by flow cytometry in cells of A. SUP-B15 and B. K562 treated with Givinostat (1mM). The cultured cells were stained with PI (see Materials and methods) at 0 to 48hrs of incubation with Givinostat. Data were one of three representative tests (Detailed data were summarized in Table 1 ). PI, Propidium Iodine. Fractions were analyzed using software Mod-Fit.
    Figure Legend Snippet: Induction of apoptosis by Givinostat on leukemia cell lines The induction of apoptosis measured by sub-G0/G1 fractions by flow cytometry in cells of A. SUP-B15 and B. K562 treated with Givinostat (1mM). The cultured cells were stained with PI (see Materials and methods) at 0 to 48hrs of incubation with Givinostat. Data were one of three representative tests (Detailed data were summarized in Table 1 ). PI, Propidium Iodine. Fractions were analyzed using software Mod-Fit.

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Incubation, Software

    Pro-apoptosis of Givinostat on leukemia cells The induction of apoptosis measured by V-FITC/PI fractions by flow cytometry in cells of A. SUP-B15 and B. K562 treated with Givinostat (0.5μM). The cultured cells were stained with 5μl of FITC Annexin V and 5μl of PI (see Materials and methods) at 24 and 48hrs of incubation with Givinostat. Data were one of three representative tests (Detailed data were summarized in Table 2 ). PI, Propidium Iodine. Fractions were analyzed using software FlowJo software (TreeStar).
    Figure Legend Snippet: Pro-apoptosis of Givinostat on leukemia cells The induction of apoptosis measured by V-FITC/PI fractions by flow cytometry in cells of A. SUP-B15 and B. K562 treated with Givinostat (0.5μM). The cultured cells were stained with 5μl of FITC Annexin V and 5μl of PI (see Materials and methods) at 24 and 48hrs of incubation with Givinostat. Data were one of three representative tests (Detailed data were summarized in Table 2 ). PI, Propidium Iodine. Fractions were analyzed using software FlowJo software (TreeStar).

    Techniques Used: Flow Cytometry, Cytometry, Cell Culture, Staining, Incubation, Software

    4) Product Images from "A Microdevice Platform Recapitulating Hypoxic Tumor Microenvironments"

    Article Title: A Microdevice Platform Recapitulating Hypoxic Tumor Microenvironments

    Journal: Scientific Reports

    doi: 10.1038/s41598-017-15583-3

    Cellular response to hypoxia-targeting drugs in hypoxia device. ( A ) Live-dead staining of micropatterned MCF-7 cells under normoxic condition or in hypoxia device, without treatment or under the treatment of tirapazamine (TPZ), a drug targeting hypoxic cells. Green: live (calcein); red: dead (propidium iodide). ( B ) Areal density of live (green) and dead (red) cells in the micropattern along the radial direction. ( C ) Proportion of live cells in the inner/pillar region versus outer region (N = 3). One-way ANOVA; n.s.: not significant (p > 0.05). Error bars: SD.
    Figure Legend Snippet: Cellular response to hypoxia-targeting drugs in hypoxia device. ( A ) Live-dead staining of micropatterned MCF-7 cells under normoxic condition or in hypoxia device, without treatment or under the treatment of tirapazamine (TPZ), a drug targeting hypoxic cells. Green: live (calcein); red: dead (propidium iodide). ( B ) Areal density of live (green) and dead (red) cells in the micropattern along the radial direction. ( C ) Proportion of live cells in the inner/pillar region versus outer region (N = 3). One-way ANOVA; n.s.: not significant (p > 0.05). Error bars: SD.

    Techniques Used: Staining

    5) Product Images from "Neuroprotection by Brazilian Green Propolis against In vitro and In vivo Ischemic Neuronal Damage"

    Article Title: Neuroprotection by Brazilian Green Propolis against In vitro and In vivo Ischemic Neuronal Damage

    Journal: Evidence-based Complementary and Alternative Medicine

    doi: 10.1093/ecam/neh078

    Typical photographs illustrating the effect of propolis on serum deprivation-induced cell damage in PC12 cell cultures [Hoechst 33342 and propidium iodide (PI) single or dual staining]. Differentiated PC12 cells were immersed in serum-free DMEM supplemented with 0.1% BSA, and then propolis was added to the cell cultures. Cells were maintained in this condition for 2 days. Viable cells are Hoechst 33342-positive and PI-negative, whereas dead cells are Hoechst 33342-positive and PI-positive. At 4 μg/ml, propolis (extract with ethanol) decreased the number of cells stained by PI (versus vehicle treatment). (A, D and H) Control (vehicle treatment). (B, E and I) Vehicle treatment + serum deprivation. (C, F and J) Propolis treatment + serum deprivation. (A–C) Hoechst 33342 staining. (D–F) PI staining. (G–I) Merged images (Hoechst 33342 + PI dual staining).
    Figure Legend Snippet: Typical photographs illustrating the effect of propolis on serum deprivation-induced cell damage in PC12 cell cultures [Hoechst 33342 and propidium iodide (PI) single or dual staining]. Differentiated PC12 cells were immersed in serum-free DMEM supplemented with 0.1% BSA, and then propolis was added to the cell cultures. Cells were maintained in this condition for 2 days. Viable cells are Hoechst 33342-positive and PI-negative, whereas dead cells are Hoechst 33342-positive and PI-positive. At 4 μg/ml, propolis (extract with ethanol) decreased the number of cells stained by PI (versus vehicle treatment). (A, D and H) Control (vehicle treatment). (B, E and I) Vehicle treatment + serum deprivation. (C, F and J) Propolis treatment + serum deprivation. (A–C) Hoechst 33342 staining. (D–F) PI staining. (G–I) Merged images (Hoechst 33342 + PI dual staining).

    Techniques Used: Staining

    Related Articles

    Flow Cytometry:

    Article Title: Low-Concentration PTX And RSL3 Inhibits Tumor Cell Growth Synergistically By Inducing Ferroptosis In Mutant p53 Hypopharyngeal Squamous Carcinoma
    Article Snippet: After incubation with Sheep Anti-Rabbit-IgG-HRP (ab6747) at or Sheep Anti-Mouse-IgG-HRP (ab6808) at 1:4000 dilution for 1 hr at room temperature, proteins were visualized by enhanced chemiluminescence (Pierce, Rockford, IL, USA, #32106) followed by exposure to standard X-ray films. .. Lipid ROS Measurement By Flow Cytometry Lipid Peroxidation Sensor BODIPY® 581/591 (Cat: D3861, Thermo Fisher) was used to detect reactive oxygen species (ROS) in cells and membranes according to the manufacture introduction. .. After HPSC cells were treated as indicated, C11-BODIPY was added (2.5 μL/mL) and incubated for 10 min.

    Article Title: Arginine Deiminase Induces Immunogenic Cell Death and Is Enhanced by N-acetylcysteine in Murine MC38 Colorectal Cancer Cells and MDA-MB-231 Human Breast Cancer Cells In Vitro
    Article Snippet: Reversible Ponceau staining of membranes was performed to check the equal loading of supernatants. .. Detection of Cell Surface-Translocated CRTCells were trypsinized and washed twice with FACS buffer, and then incubated with anti-CRT antibody (Abcam, Cambridge, MA, USA) at 1:200 dilution in 100 μL FACS buffer at 4 °C for 1 h. Then, cells were washed and stained with FITC-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 30 min, followed by staining with propidium iodide (PI, Invitrogen) and acquisition by flow cytometry, and the fluorescent intensity of stained live cells was gated on propidium iodide (PI)-negative cells. .. ATP DetectionATP levels in culture medium were detected using the ENLITEN ATP Assay kit (Promega, Madison, WI, USA), following the manufacturer’s instructions, and light intensity was measured using a microplate reader (Molecular Devices, San Jose, CA, USA).

    Article Title: Localized immune tolerance from FasL-functionalized PLG scaffolds
    Article Snippet: To assess the ability for FasL particles or scaffolds to induce apoptosis in vitro, 1 mg/mL particles or a single scaffold was added to a 96 well plate containing A20 cells (mouse B lymphoma) at a concentration of 1.5×106 cells/mL and incubated for 18 hours. .. Cells were removed from the plate, stained with annexin V and propidium iodide (Life Technologies), and analyzed via flow cytometry. .. C57BL/6 ( H-2 b ) and BALB/c ( H-2 d ) mice were purchased from Jackson Laboratory and bred in our specific pathogen-free animal facility at the University of Louisville using protocols approved by the Institutional Animal Care and Use Committee.

    Article Title: RGD-conjugated gold nanorods induce radiosensitization in melanoma cancer cells by downregulating ?v?3 expression
    Article Snippet: Cellular DNA content and cell cycle data were analyzed by flow cytometry using multicycle system 2.0 software. .. For the apoptosis assay, the cells were stained with Annexin-V-fluorescein isothiocyanate/propidium iodide (Invitrogen) and measured by flow cytometry. .. Integrin αv β3 analysis A375 cells were collected and suspended in phosphate-buffered saline/0.2% bovine serum albumin at a concentration of 106 cells/mL.

    Cytometry:

    Article Title: Low-Concentration PTX And RSL3 Inhibits Tumor Cell Growth Synergistically By Inducing Ferroptosis In Mutant p53 Hypopharyngeal Squamous Carcinoma
    Article Snippet: After incubation with Sheep Anti-Rabbit-IgG-HRP (ab6747) at or Sheep Anti-Mouse-IgG-HRP (ab6808) at 1:4000 dilution for 1 hr at room temperature, proteins were visualized by enhanced chemiluminescence (Pierce, Rockford, IL, USA, #32106) followed by exposure to standard X-ray films. .. Lipid ROS Measurement By Flow Cytometry Lipid Peroxidation Sensor BODIPY® 581/591 (Cat: D3861, Thermo Fisher) was used to detect reactive oxygen species (ROS) in cells and membranes according to the manufacture introduction. .. After HPSC cells were treated as indicated, C11-BODIPY was added (2.5 μL/mL) and incubated for 10 min.

    Article Title: Localized immune tolerance from FasL-functionalized PLG scaffolds
    Article Snippet: To assess the ability for FasL particles or scaffolds to induce apoptosis in vitro, 1 mg/mL particles or a single scaffold was added to a 96 well plate containing A20 cells (mouse B lymphoma) at a concentration of 1.5×106 cells/mL and incubated for 18 hours. .. Cells were removed from the plate, stained with annexin V and propidium iodide (Life Technologies), and analyzed via flow cytometry. .. C57BL/6 ( H-2 b ) and BALB/c ( H-2 d ) mice were purchased from Jackson Laboratory and bred in our specific pathogen-free animal facility at the University of Louisville using protocols approved by the Institutional Animal Care and Use Committee.

    Article Title: RGD-conjugated gold nanorods induce radiosensitization in melanoma cancer cells by downregulating ?v?3 expression
    Article Snippet: Cellular DNA content and cell cycle data were analyzed by flow cytometry using multicycle system 2.0 software. .. For the apoptosis assay, the cells were stained with Annexin-V-fluorescein isothiocyanate/propidium iodide (Invitrogen) and measured by flow cytometry. .. Integrin αv β3 analysis A375 cells were collected and suspended in phosphate-buffered saline/0.2% bovine serum albumin at a concentration of 106 cells/mL.

    FACS:

    Article Title: Arginine Deiminase Induces Immunogenic Cell Death and Is Enhanced by N-acetylcysteine in Murine MC38 Colorectal Cancer Cells and MDA-MB-231 Human Breast Cancer Cells In Vitro
    Article Snippet: Reversible Ponceau staining of membranes was performed to check the equal loading of supernatants. .. Detection of Cell Surface-Translocated CRTCells were trypsinized and washed twice with FACS buffer, and then incubated with anti-CRT antibody (Abcam, Cambridge, MA, USA) at 1:200 dilution in 100 μL FACS buffer at 4 °C for 1 h. Then, cells were washed and stained with FITC-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 30 min, followed by staining with propidium iodide (PI, Invitrogen) and acquisition by flow cytometry, and the fluorescent intensity of stained live cells was gated on propidium iodide (PI)-negative cells. .. ATP DetectionATP levels in culture medium were detected using the ENLITEN ATP Assay kit (Promega, Madison, WI, USA), following the manufacturer’s instructions, and light intensity was measured using a microplate reader (Molecular Devices, San Jose, CA, USA).

    Incubation:

    Article Title: Arginine Deiminase Induces Immunogenic Cell Death and Is Enhanced by N-acetylcysteine in Murine MC38 Colorectal Cancer Cells and MDA-MB-231 Human Breast Cancer Cells In Vitro
    Article Snippet: Reversible Ponceau staining of membranes was performed to check the equal loading of supernatants. .. Detection of Cell Surface-Translocated CRTCells were trypsinized and washed twice with FACS buffer, and then incubated with anti-CRT antibody (Abcam, Cambridge, MA, USA) at 1:200 dilution in 100 μL FACS buffer at 4 °C for 1 h. Then, cells were washed and stained with FITC-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 30 min, followed by staining with propidium iodide (PI, Invitrogen) and acquisition by flow cytometry, and the fluorescent intensity of stained live cells was gated on propidium iodide (PI)-negative cells. .. ATP DetectionATP levels in culture medium were detected using the ENLITEN ATP Assay kit (Promega, Madison, WI, USA), following the manufacturer’s instructions, and light intensity was measured using a microplate reader (Molecular Devices, San Jose, CA, USA).

    Staining:

    Article Title: Arginine Deiminase Induces Immunogenic Cell Death and Is Enhanced by N-acetylcysteine in Murine MC38 Colorectal Cancer Cells and MDA-MB-231 Human Breast Cancer Cells In Vitro
    Article Snippet: Reversible Ponceau staining of membranes was performed to check the equal loading of supernatants. .. Detection of Cell Surface-Translocated CRTCells were trypsinized and washed twice with FACS buffer, and then incubated with anti-CRT antibody (Abcam, Cambridge, MA, USA) at 1:200 dilution in 100 μL FACS buffer at 4 °C for 1 h. Then, cells were washed and stained with FITC-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 30 min, followed by staining with propidium iodide (PI, Invitrogen) and acquisition by flow cytometry, and the fluorescent intensity of stained live cells was gated on propidium iodide (PI)-negative cells. .. ATP DetectionATP levels in culture medium were detected using the ENLITEN ATP Assay kit (Promega, Madison, WI, USA), following the manufacturer’s instructions, and light intensity was measured using a microplate reader (Molecular Devices, San Jose, CA, USA).

    Article Title: Localized immune tolerance from FasL-functionalized PLG scaffolds
    Article Snippet: To assess the ability for FasL particles or scaffolds to induce apoptosis in vitro, 1 mg/mL particles or a single scaffold was added to a 96 well plate containing A20 cells (mouse B lymphoma) at a concentration of 1.5×106 cells/mL and incubated for 18 hours. .. Cells were removed from the plate, stained with annexin V and propidium iodide (Life Technologies), and analyzed via flow cytometry. .. C57BL/6 ( H-2 b ) and BALB/c ( H-2 d ) mice were purchased from Jackson Laboratory and bred in our specific pathogen-free animal facility at the University of Louisville using protocols approved by the Institutional Animal Care and Use Committee.

    Article Title: Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing
    Article Snippet: Incubation overnight at 4°C with α-dimethyl H3-K9 antibody (Upstate; 1:100 in PBS/0.1% Triton X-100) was followed by incubation with secondary FITC-conjugated antibody (Jackson Immunoresearch Laboratories Inc.; 1:100 in PBS/0.1% Triton X-100) for 2 h at 37°C. .. DNA was stained with propidium iodide (Molecular Probes). .. Preparations were examined with confocal laser scanning microscopy.

    Article Title: RGD-conjugated gold nanorods induce radiosensitization in melanoma cancer cells by downregulating ?v?3 expression
    Article Snippet: Cellular DNA content and cell cycle data were analyzed by flow cytometry using multicycle system 2.0 software. .. For the apoptosis assay, the cells were stained with Annexin-V-fluorescein isothiocyanate/propidium iodide (Invitrogen) and measured by flow cytometry. .. Integrin αv β3 analysis A375 cells were collected and suspended in phosphate-buffered saline/0.2% bovine serum albumin at a concentration of 106 cells/mL.

    Apoptosis Assay:

    Article Title: RGD-conjugated gold nanorods induce radiosensitization in melanoma cancer cells by downregulating ?v?3 expression
    Article Snippet: Cellular DNA content and cell cycle data were analyzed by flow cytometry using multicycle system 2.0 software. .. For the apoptosis assay, the cells were stained with Annexin-V-fluorescein isothiocyanate/propidium iodide (Invitrogen) and measured by flow cytometry. .. Integrin αv β3 analysis A375 cells were collected and suspended in phosphate-buffered saline/0.2% bovine serum albumin at a concentration of 106 cells/mL.

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  • 97
    Thermo Fisher propidium iodide
    Apoptosis induction by ADI-PEG 20 and NAC. A summary of early (Annexin+/PI−) and late (Annexin+/PI+) apoptotic cell death after 24 h ( A ) and 48 h ( B ) of treatment of MC38 cells and MDA-MB-231 cells for 24 h ( C ) and 48 h ( D ). The x -axis represents Annexin V-FITC staining, and the y -axis represents PI staining. Each experiment was conducted in triplicate. Ctrl: no treatment control; PI: <t>propidium</t> iodide.
    Propidium Iodide, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/propidium iodide/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    propidium iodide - by Bioz Stars, 2021-04
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    99
    Thermo Fisher annexin v assay kit
    Four MM cell lines were cultured in presence of BMSCs and MM cytokines (interleukin-6 (IL-6), vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1)) at the concentrations of 10 ng/ml. ( a ) The percentage of apoptosis in MM cells (aspirated away from the BMSC) was determined using <t>Annexin</t> V assay and flow cytometry at 24 h after the treatment with BSO (400 μ M ) and L-PAM (30 μ M ). Bars represent percentage of cell undergoing apoptosis (Annexin V+ and PI+/−). Error bars represent s.d. ( n ⩾3) and asterisk represent statistical difference in means ( P
    Annexin V Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v assay kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    annexin v assay kit - by Bioz Stars, 2021-04
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    Image Search Results


    Apoptosis induction by ADI-PEG 20 and NAC. A summary of early (Annexin+/PI−) and late (Annexin+/PI+) apoptotic cell death after 24 h ( A ) and 48 h ( B ) of treatment of MC38 cells and MDA-MB-231 cells for 24 h ( C ) and 48 h ( D ). The x -axis represents Annexin V-FITC staining, and the y -axis represents PI staining. Each experiment was conducted in triplicate. Ctrl: no treatment control; PI: propidium iodide.

    Journal: Molecules

    Article Title: Arginine Deiminase Induces Immunogenic Cell Death and Is Enhanced by N-acetylcysteine in Murine MC38 Colorectal Cancer Cells and MDA-MB-231 Human Breast Cancer Cells In Vitro

    doi: 10.3390/molecules26020511

    Figure Lengend Snippet: Apoptosis induction by ADI-PEG 20 and NAC. A summary of early (Annexin+/PI−) and late (Annexin+/PI+) apoptotic cell death after 24 h ( A ) and 48 h ( B ) of treatment of MC38 cells and MDA-MB-231 cells for 24 h ( C ) and 48 h ( D ). The x -axis represents Annexin V-FITC staining, and the y -axis represents PI staining. Each experiment was conducted in triplicate. Ctrl: no treatment control; PI: propidium iodide.

    Article Snippet: Detection of Cell Surface-Translocated CRTCells were trypsinized and washed twice with FACS buffer, and then incubated with anti-CRT antibody (Abcam, Cambridge, MA, USA) at 1:200 dilution in 100 μL FACS buffer at 4 °C for 1 h. Then, cells were washed and stained with FITC-conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 30 min, followed by staining with propidium iodide (PI, Invitrogen) and acquisition by flow cytometry, and the fluorescent intensity of stained live cells was gated on propidium iodide (PI)-negative cells.

    Techniques: Multiple Displacement Amplification, Staining

    Orientation of cells in the arterial wall. A, Cartoon of arterial wall with circumferentially oriented smooth muscle cells ( SMC s) vertically aligned. B, In most arteries fixed and stained with phalloidin (yellow) the SMC s were adjacent and vertically aligned, but in some cases the orientation was inconsistent and sparse. This misalignment occurred in arteries obtained both pre‐ and post‐ CPB , here shown in the pre‐ CPB artery; both arteries are from Patient 10. The nuclei were stained with propidium iodide (blue), and elastin with AF ‐633 (pink, lower panels). CPB indicates cardiopulmonary bypass. See Video S1 for a z ‐stack through the wall of an artery stained with AF ‐633.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Isolated Human Pulmonary Artery Structure and Function Pre‐ and Post‐Cardiopulmonary Bypass Surgery

    doi: 10.1161/JAHA.115.002822

    Figure Lengend Snippet: Orientation of cells in the arterial wall. A, Cartoon of arterial wall with circumferentially oriented smooth muscle cells ( SMC s) vertically aligned. B, In most arteries fixed and stained with phalloidin (yellow) the SMC s were adjacent and vertically aligned, but in some cases the orientation was inconsistent and sparse. This misalignment occurred in arteries obtained both pre‐ and post‐ CPB , here shown in the pre‐ CPB artery; both arteries are from Patient 10. The nuclei were stained with propidium iodide (blue), and elastin with AF ‐633 (pink, lower panels). CPB indicates cardiopulmonary bypass. See Video S1 for a z ‐stack through the wall of an artery stained with AF ‐633.

    Article Snippet: The structural dyes Hoechst (H3570), PI (P1304MP), DAPI (D3571), and AF‐633 (A30634) were purchased from ThermoFisher Scientific (Paisley, UK) and phalloidin‐TRITC from Sigma‐Aldrich (Dorset, UK).

    Techniques: Staining

    Live/dead staining in cannulated human pulmonary arteries. The cytosolic marker calcein AM is only de‐esterified in live cells (Calcein, green). The cell permeant dye Hoechst 33342 (Hoechst, cyan) stains all nuclei, whereas the cell impermeant propidium iodide ( PI , pink) only penetrates dead cell membranes, co‐localized nuclear stains are purple and also reflect dead cells. Trans, transmitted light image. A, By focusing through the wall of the artery from a pre‐ CPB biopsy (Patient 2), it was clear that there were no dead smooth muscle cells ( SMC s, left panels, vertical orientation, one cell outlined with dashed line, no SMC s at the right of the image indicated by asterisk); and very few dead endothelial cells ( EC s, right panels, near‐horizontal orientation, one cell outlined with dashed line). B, In contrast, the wall of the artery from a post‐ CPB biopsy (Patient 10) was littered with dead SMC s and no live EC s were present. C, Scatter plots showing individual data points of the percentage of cells taking up calcein and not PI (Live cells) from 7 pre‐ CPB biopsies, and 5 post‐ CPB biopsies along with the mean±SEM. In paired comparisons there was no significant difference between pre vs post‐ CPB cells (n=5, Wilcoxon matched‐paired signed‐rank test). D, Overall, the staining for live SMC s tended to reflect the ability of U46619 to stimulate vasoconstriction ( VC ), whereas the presence of live EC s did not uniformly reflect an ability of bradykinin to stimulate vasodilation ( VD ). Arrowheads indicate the same position in all images within a column. See Videos S2 and S3 for z ‐stacks through these arteries. Yellow, gray, and cyan‐filled symbols correspond to arteries from patients 2, 8, and 10, respectively (paired data in both C and D). Purple symbols correspond to arteries from patient 4 (paired data in C). CPB indicates cardiopulmonary bypass; numbers in parenthesis indicate the number of equal values.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Isolated Human Pulmonary Artery Structure and Function Pre‐ and Post‐Cardiopulmonary Bypass Surgery

    doi: 10.1161/JAHA.115.002822

    Figure Lengend Snippet: Live/dead staining in cannulated human pulmonary arteries. The cytosolic marker calcein AM is only de‐esterified in live cells (Calcein, green). The cell permeant dye Hoechst 33342 (Hoechst, cyan) stains all nuclei, whereas the cell impermeant propidium iodide ( PI , pink) only penetrates dead cell membranes, co‐localized nuclear stains are purple and also reflect dead cells. Trans, transmitted light image. A, By focusing through the wall of the artery from a pre‐ CPB biopsy (Patient 2), it was clear that there were no dead smooth muscle cells ( SMC s, left panels, vertical orientation, one cell outlined with dashed line, no SMC s at the right of the image indicated by asterisk); and very few dead endothelial cells ( EC s, right panels, near‐horizontal orientation, one cell outlined with dashed line). B, In contrast, the wall of the artery from a post‐ CPB biopsy (Patient 10) was littered with dead SMC s and no live EC s were present. C, Scatter plots showing individual data points of the percentage of cells taking up calcein and not PI (Live cells) from 7 pre‐ CPB biopsies, and 5 post‐ CPB biopsies along with the mean±SEM. In paired comparisons there was no significant difference between pre vs post‐ CPB cells (n=5, Wilcoxon matched‐paired signed‐rank test). D, Overall, the staining for live SMC s tended to reflect the ability of U46619 to stimulate vasoconstriction ( VC ), whereas the presence of live EC s did not uniformly reflect an ability of bradykinin to stimulate vasodilation ( VD ). Arrowheads indicate the same position in all images within a column. See Videos S2 and S3 for z ‐stacks through these arteries. Yellow, gray, and cyan‐filled symbols correspond to arteries from patients 2, 8, and 10, respectively (paired data in both C and D). Purple symbols correspond to arteries from patient 4 (paired data in C). CPB indicates cardiopulmonary bypass; numbers in parenthesis indicate the number of equal values.

    Article Snippet: The structural dyes Hoechst (H3570), PI (P1304MP), DAPI (D3571), and AF‐633 (A30634) were purchased from ThermoFisher Scientific (Paisley, UK) and phalloidin‐TRITC from Sigma‐Aldrich (Dorset, UK).

    Techniques: Staining, Marker

    Triterpene seco -acids promote apoptosis in AGS and DLD-1 cells. AGS and DLD-1 cells treated with 3,4- seco -urs-4(23),20(30)-dien-3-oic acid ( 1 ), 3,4- seco -olean-4(24)-en-19-oxo-3-oic acid ( 2 ), and 3,4- seco -urs-4(23),20(30)-dien-19-ol-3-oic acid ( 3 ) for 24 h were triple stained with annexin V-FITC conjugate (green fluorescence), propidium iodide (red fluorescence), and Hoechst 33342 (blue fluorescence), and were visualized by fluorescence microscopy. Representative merged photographs are shown. Scale bar = 100 μm. Data are presented as mean ± standard deviation (SD) from three assays. * p

    Journal: Molecules

    Article Title: Cytotoxicity of Triterpene Seco-Acids from Betula pubescens Buds

    doi: 10.3390/molecules24224060

    Figure Lengend Snippet: Triterpene seco -acids promote apoptosis in AGS and DLD-1 cells. AGS and DLD-1 cells treated with 3,4- seco -urs-4(23),20(30)-dien-3-oic acid ( 1 ), 3,4- seco -olean-4(24)-en-19-oxo-3-oic acid ( 2 ), and 3,4- seco -urs-4(23),20(30)-dien-19-ol-3-oic acid ( 3 ) for 24 h were triple stained with annexin V-FITC conjugate (green fluorescence), propidium iodide (red fluorescence), and Hoechst 33342 (blue fluorescence), and were visualized by fluorescence microscopy. Representative merged photographs are shown. Scale bar = 100 μm. Data are presented as mean ± standard deviation (SD) from three assays. * p

    Article Snippet: After 24 h, the medium was changed and triterpene seco -acids were added for the next 24 h. Floating and adherent cells were collected and assayed with a Dead Cell Apoptosis Kit with annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) for flow cytometry (Thermo Fisher Scientific, Warszawa, Poland), according to the manufacturer’s protocol, as described in [ ].

    Techniques: Staining, Fluorescence, Microscopy, Standard Deviation

    Four MM cell lines were cultured in presence of BMSCs and MM cytokines (interleukin-6 (IL-6), vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1)) at the concentrations of 10 ng/ml. ( a ) The percentage of apoptosis in MM cells (aspirated away from the BMSC) was determined using Annexin V assay and flow cytometry at 24 h after the treatment with BSO (400 μ M ) and L-PAM (30 μ M ). Bars represent percentage of cell undergoing apoptosis (Annexin V+ and PI+/−). Error bars represent s.d. ( n ⩾3) and asterisk represent statistical difference in means ( P

    Journal: Blood Cancer Journal

    Article Title: The glutathione synthesis inhibitor buthionine sulfoximine synergistically enhanced melphalan activity against preclinical models of multiple myeloma

    doi: 10.1038/bcj.2014.45

    Figure Lengend Snippet: Four MM cell lines were cultured in presence of BMSCs and MM cytokines (interleukin-6 (IL-6), vascular endothelial growth factor (VEGF) and insulin-like growth factor-1 (IGF-1)) at the concentrations of 10 ng/ml. ( a ) The percentage of apoptosis in MM cells (aspirated away from the BMSC) was determined using Annexin V assay and flow cytometry at 24 h after the treatment with BSO (400 μ M ) and L-PAM (30 μ M ). Bars represent percentage of cell undergoing apoptosis (Annexin V+ and PI+/−). Error bars represent s.d. ( n ⩾3) and asterisk represent statistical difference in means ( P

    Article Snippet: Interleukin-6, vascular endothelial growth factor, insulin-like growth factor-1 and Annexin V assay kit were from Life Technologies (Carlsbad, CA, USA).

    Techniques: Cell Culture, Annexin V Assay, Flow Cytometry, Cytometry