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Becton Dickinson propidium iodide pi
The effects of vitamin C on human T cell apoptosis. Cells were activated with phorbol-12-myristate-13-acetate/ionomycin, added with 0.5 mM vitamin C 2 hours before or 24 hours after activation, stained with Annexin V and <t>propidium</t> iodide (PI), and subjected to flow cytometric analysis. The experiment was repeated three times and a representative one is shown. Control group was cultured without vitamin C treatment.
Propidium Iodide Pi, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Vitamin C is taken up by human T cells via sodium-dependent vitamin C transporter 2 (SVCT2) and exerts inhibitory effects on the activation of these cells in vitro"

Article Title: Vitamin C is taken up by human T cells via sodium-dependent vitamin C transporter 2 (SVCT2) and exerts inhibitory effects on the activation of these cells in vitro

Journal: Anatomy & Cell Biology

doi: 10.5115/acb.2016.49.2.88

The effects of vitamin C on human T cell apoptosis. Cells were activated with phorbol-12-myristate-13-acetate/ionomycin, added with 0.5 mM vitamin C 2 hours before or 24 hours after activation, stained with Annexin V and propidium iodide (PI), and subjected to flow cytometric analysis. The experiment was repeated three times and a representative one is shown. Control group was cultured without vitamin C treatment.
Figure Legend Snippet: The effects of vitamin C on human T cell apoptosis. Cells were activated with phorbol-12-myristate-13-acetate/ionomycin, added with 0.5 mM vitamin C 2 hours before or 24 hours after activation, stained with Annexin V and propidium iodide (PI), and subjected to flow cytometric analysis. The experiment was repeated three times and a representative one is shown. Control group was cultured without vitamin C treatment.

Techniques Used: Activation Assay, Staining, Flow Cytometry, Cell Culture

2) Product Images from "Knockdown of E2F3 Inhibits Proliferation, Migration, and Invasion and Increases Apoptosis in Glioma Cells"

Article Title: Knockdown of E2F3 Inhibits Proliferation, Migration, and Invasion and Increases Apoptosis in Glioma Cells

Journal: Oncology Research

doi: 10.3727/096504017X14897158009178

Suppressing E2F3a expression induced apoptosis in glioma cells. U373 cells were infected with E2F3a-shRNA and collected 48 h later, and U251 cells were infected with E2F3a constructs and collected 48 h later. Cell apoptosis of U373 (A, C) and U251 cells (B, D) was analyzed by annexin V/propidium iodide (PI) staining. *** p
Figure Legend Snippet: Suppressing E2F3a expression induced apoptosis in glioma cells. U373 cells were infected with E2F3a-shRNA and collected 48 h later, and U251 cells were infected with E2F3a constructs and collected 48 h later. Cell apoptosis of U373 (A, C) and U251 cells (B, D) was analyzed by annexin V/propidium iodide (PI) staining. *** p

Techniques Used: Expressing, Infection, shRNA, Construct, Staining

3) Product Images from "Enhanced Activity of P4503A4 and UGT1A10 Induced by Acridinone Derivatives C-1305 and C-1311 in MCF-7 and HCT116 Cancer Cells: Consequences for the Drugs’ Cytotoxicity, Metabolism and Cellular Response"

Article Title: Enhanced Activity of P4503A4 and UGT1A10 Induced by Acridinone Derivatives C-1305 and C-1311 in MCF-7 and HCT116 Cancer Cells: Consequences for the Drugs’ Cytotoxicity, Metabolism and Cellular Response

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms21113954

Cell cycle distribution of MCF-7-EV and HCT116-EV cells. Cells were untreated (control) or treated with IC 80 dosage for MCF-7 and IC 90 for HCT116 cells of C-1305 or C-1311 compounds for the times indicated and subjected to propidium iodide staining and flow cytometry as described in Materials and Methods. Histograms show number of cells ( y -axis) versus DNA content ( x -axis) and are representative of at least three experiments for each condition. Bar graphs show data quantitation with percentage of cells with less than 2N DNA (sub-G1 fraction).
Figure Legend Snippet: Cell cycle distribution of MCF-7-EV and HCT116-EV cells. Cells were untreated (control) or treated with IC 80 dosage for MCF-7 and IC 90 for HCT116 cells of C-1305 or C-1311 compounds for the times indicated and subjected to propidium iodide staining and flow cytometry as described in Materials and Methods. Histograms show number of cells ( y -axis) versus DNA content ( x -axis) and are representative of at least three experiments for each condition. Bar graphs show data quantitation with percentage of cells with less than 2N DNA (sub-G1 fraction).

Techniques Used: Staining, Flow Cytometry, Quantitation Assay

Cell cycle distribution of MCF-7-CYP3A4, MCF-7-UGT1A10, HCT116-CYP3A4 and HCT116-UGT1A10 cells. Cells were untreated (control) or treated with C-1305 or C-1311 (IC 80 for MCF-7 cells, IC 90 for HCT116) for the times indicated and subjected to propidium iodide staining and flow cytometry as described in Materials and Methods. ( A ) Histograms show number of cells ( y -axis) versus DNA content ( x -axis) and are representative of at least three experiments for each condition. ( B , C ) Bar graphs show data quantitation with percentage of cells with less than 2N DNA (sub-G1 fraction): three types of MCF-7 ( B ) and HCT116 ( C ) cells treated with C-1305 or C-1311. Results are expressed as mean ± SEM ( n ≥ 3). Significant differences in cell percentages between control, stably transfected with EV cells and UGT1A10- or CYP3A4-transfected are indicated as follows: * p ≤ 0.05; ** p ≤ 0.001.
Figure Legend Snippet: Cell cycle distribution of MCF-7-CYP3A4, MCF-7-UGT1A10, HCT116-CYP3A4 and HCT116-UGT1A10 cells. Cells were untreated (control) or treated with C-1305 or C-1311 (IC 80 for MCF-7 cells, IC 90 for HCT116) for the times indicated and subjected to propidium iodide staining and flow cytometry as described in Materials and Methods. ( A ) Histograms show number of cells ( y -axis) versus DNA content ( x -axis) and are representative of at least three experiments for each condition. ( B , C ) Bar graphs show data quantitation with percentage of cells with less than 2N DNA (sub-G1 fraction): three types of MCF-7 ( B ) and HCT116 ( C ) cells treated with C-1305 or C-1311. Results are expressed as mean ± SEM ( n ≥ 3). Significant differences in cell percentages between control, stably transfected with EV cells and UGT1A10- or CYP3A4-transfected are indicated as follows: * p ≤ 0.05; ** p ≤ 0.001.

Techniques Used: Staining, Flow Cytometry, Quantitation Assay, Stable Transfection, Transfection

4) Product Images from "Integrative Transcriptomic Network Analysis of Butyrate Treated Colorectal Cancer Cells"

Article Title: Integrative Transcriptomic Network Analysis of Butyrate Treated Colorectal Cancer Cells

Journal: Cancers

doi: 10.3390/cancers13040636

EIF4G2 siRNA knockdown efficiency in HCT116 cells. mRNA levels of EIF4G2 in CRC cells ( A ) HCT116 cells transfected with NC siRNA or EIF4G2 siRNA for 72 h. The mean mRNA levels ± SEM ( n = 3) are represented, and their expression is normalized to the geometric mean of three reference genes, ACTB, B2M and GAPDH. Real-time cell index measurements using the xCELLigence RTCA platform, in ( B ) HCT116 cells transfected with NC or EIF4G2 siRNA for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. ( C ) The mean ± SEM ( n = 4) is shown at 72 h post-transfection with EIF4G2 siRNA. ( D ) Flow cytometry analysis of the cell cycle in siRNA transfected HCT116 cells after 24 h of butyrate treatment. Bar charts showing the cell cycle analysis of HCT116 cells reverse-transfected with EIF4G2 siRNAs for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. Cells were stained with propidium iodide, and cell percentage measured using the Cytoflex flow cytometer. The mean ± SEM ( n = 3) is shown. Significant results are indicated by * p
Figure Legend Snippet: EIF4G2 siRNA knockdown efficiency in HCT116 cells. mRNA levels of EIF4G2 in CRC cells ( A ) HCT116 cells transfected with NC siRNA or EIF4G2 siRNA for 72 h. The mean mRNA levels ± SEM ( n = 3) are represented, and their expression is normalized to the geometric mean of three reference genes, ACTB, B2M and GAPDH. Real-time cell index measurements using the xCELLigence RTCA platform, in ( B ) HCT116 cells transfected with NC or EIF4G2 siRNA for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. ( C ) The mean ± SEM ( n = 4) is shown at 72 h post-transfection with EIF4G2 siRNA. ( D ) Flow cytometry analysis of the cell cycle in siRNA transfected HCT116 cells after 24 h of butyrate treatment. Bar charts showing the cell cycle analysis of HCT116 cells reverse-transfected with EIF4G2 siRNAs for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. Cells were stained with propidium iodide, and cell percentage measured using the Cytoflex flow cytometer. The mean ± SEM ( n = 3) is shown. Significant results are indicated by * p

Techniques Used: Transfection, Expressing, Flow Cytometry, Cell Cycle Assay, Staining

Flow cytometry analysis of the cell cycle in miRNA-transfected HCT116 cells after 24 h of butyrate treatment. ( A ) Examples of flow charts depicting cell cycle analyses of HCT116 cells reverse-transfected with control (NC) or miR-542 mimics for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. ( B ) Bar charts for cell cycle analysis of HCT116 cells reverse-transfected with miRNA mimics miR-139 or miR-542 for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. Cells were stained with propidium iodide, and cell percentage measured using the Cytoflex flow cytometer. The mean ± SEM ( n = 3) is shown. Significant results are indicated by * p
Figure Legend Snippet: Flow cytometry analysis of the cell cycle in miRNA-transfected HCT116 cells after 24 h of butyrate treatment. ( A ) Examples of flow charts depicting cell cycle analyses of HCT116 cells reverse-transfected with control (NC) or miR-542 mimics for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. ( B ) Bar charts for cell cycle analysis of HCT116 cells reverse-transfected with miRNA mimics miR-139 or miR-542 for 48 h, followed by 24 h of treatment with 0 mM or 2.5 mM butyrate, over a 72 h post-transfection period. Cells were stained with propidium iodide, and cell percentage measured using the Cytoflex flow cytometer. The mean ± SEM ( n = 3) is shown. Significant results are indicated by * p

Techniques Used: Flow Cytometry, Transfection, Cell Cycle Assay, Staining

5) Product Images from "Nicotinamide-mediated inhibition of SIRT1 deacetylase is associated with the viability of cancer cells exposed to antitumor agents and apoptosis"

Article Title: Nicotinamide-mediated inhibition of SIRT1 deacetylase is associated with the viability of cancer cells exposed to antitumor agents and apoptosis

Journal: Oncology Letters

doi: 10.3892/ol.2013.1400

Indications of NAM-induced apoptosis in MCF-7 cells (all occurred in a time-dependent manner). (A) Chromatin condensation and apoptotic bodies (as indicated by white arrow), detected by Hoechst 33342 staining. (B) Increased sub-G 1 cells population, detected by flow cytometry. (C) Increased Annexin V protein, detected by Annexin V + /PI − staining. NAM, nicotinamide; PI, propidium iodide.
Figure Legend Snippet: Indications of NAM-induced apoptosis in MCF-7 cells (all occurred in a time-dependent manner). (A) Chromatin condensation and apoptotic bodies (as indicated by white arrow), detected by Hoechst 33342 staining. (B) Increased sub-G 1 cells population, detected by flow cytometry. (C) Increased Annexin V protein, detected by Annexin V + /PI − staining. NAM, nicotinamide; PI, propidium iodide.

Techniques Used: Staining, Flow Cytometry, Cytometry

6) Product Images from "Ilimaquinone Induces Apoptosis and Autophagy in Human Oral Squamous Cell Carcinoma Cells"

Article Title: Ilimaquinone Induces Apoptosis and Autophagy in Human Oral Squamous Cell Carcinoma Cells

Journal: Biomedicines

doi: 10.3390/biomedicines8090296

Effect of ilimaquinone (IQ) treatment on apoptosis. ( A ) The percentage of apoptotic cells (Q2 + Q4) after dimethyl sulfoxide (DMSO) vehicle, IQ, or 50 nM staurosporine (Stauro.) treatment for 48 h. SCC4 cells were treated with either DMSO, IQ, or Stauro. in 5% fetal bovine serum (FBS)-supplemented DMEM/F12 medium for 48 h and stained with propidium iodide (PI)/annexin V. Columns, mean; bars, S.D. ( n = 4). ** p
Figure Legend Snippet: Effect of ilimaquinone (IQ) treatment on apoptosis. ( A ) The percentage of apoptotic cells (Q2 + Q4) after dimethyl sulfoxide (DMSO) vehicle, IQ, or 50 nM staurosporine (Stauro.) treatment for 48 h. SCC4 cells were treated with either DMSO, IQ, or Stauro. in 5% fetal bovine serum (FBS)-supplemented DMEM/F12 medium for 48 h and stained with propidium iodide (PI)/annexin V. Columns, mean; bars, S.D. ( n = 4). ** p

Techniques Used: Staining

Related Articles

FACS:

Article Title: Single-cell transcriptomics reveal that PD-1 mediates immune tolerance by regulating proliferation of regulatory T cells
Article Snippet: Cells were then washed three times with 2% FBS-containing PBS and analyzed on flow cytometer (BD FACSAria™ Fusion). .. Propidium iodide (PI, BD) positive dead cells were excluded for live cell analysis/sorting, and FACS data were then analyzed with the FlowJo software (Tree star). .. Total RNA was isolated from FACS-sorted cells using the RNeasy mini kit (Qiagen) and analyzed on the Agilent Tape station for RNA Integrity Numbers (RIN) prior to library preparation.

Software:

Article Title: Single-cell transcriptomics reveal that PD-1 mediates immune tolerance by regulating proliferation of regulatory T cells
Article Snippet: Cells were then washed three times with 2% FBS-containing PBS and analyzed on flow cytometer (BD FACSAria™ Fusion). .. Propidium iodide (PI, BD) positive dead cells were excluded for live cell analysis/sorting, and FACS data were then analyzed with the FlowJo software (Tree star). .. Total RNA was isolated from FACS-sorted cells using the RNeasy mini kit (Qiagen) and analyzed on the Agilent Tape station for RNA Integrity Numbers (RIN) prior to library preparation.

Protease Inhibitor:

Article Title: Pannexin-1 is required for ATP release during apoptosis but not for inflammasome activation.
Article Snippet: .. ReagentsKey reagents and their sources were as follows: Escherichia coli LPS serotype O1101:B4 (List Biological Laboratories); ATP, cycloheximide (CHX), dexamethasone, and nigericin (Sigma); z-VAD-fmk (MBL); Imject alum (Thermo Scientific); silica (US Silica); S. typhimurium flagellin (InvivoGen); Lipofectamine 2000, pcDNA3.1(+), Pacific blue annexin V, and YO-PRO-1 (Invitrogen); complete protease inhibitor mixture and DOTAP (Roche); Jo2 hamster anti-mouse CD95 and propidium iodide (PI; BD); cleaved (Asp175) and total (8G10) caspase-3 Abs (Cell Signaling); GAPDH, caspase-1 p10, and actin Abs (Santa Cruz); 4B4 caspase-1 p20 Ab (Genentech); and 3ZD anti–IL-1b and pro–IL-1b Ab (Biological Resources Branch, National Cancer Institute). ..

Incubation:

Article Title: Double-Stranded DNA Activates Glomerular Endothelial Cells and Enhances Albumin Permeability via a Toll-Like Receptor-Independent Cytosolic DNA Recognition Pathway
Article Snippet: Blots were developed with enhanced chemiluminescence (Amersham Pharmacia, Freiburg, Germany). .. B-DNA-stimulated GEnCs were washed with PBS and incubated with binding buffer containing either FITC-anti-annexin V (BD, Franklin Lakes, NJ) or propidium iodide (PI, BD) for 15 minutes at room temperature. .. In other experiments, cells were then washed with PBS and incubated with PBS containing FITC-anti-CD106 (VCAM-1) from BioLegend (San Diego, CA) or FITC-anti-CD54 (ICAM-1) from BioLegend for 45 minutes at room temperature The cells were analyzed by flow cytometry (FACS Calibur, BD) with acquisition of 30,000 events/sample.

Article Title: TL1A/TNFR2 Axis Enhances Immunoregulatory Effects of Bone Marrow Derived Mesenchymal Stem Cell by Indian Hedgehog Signaling Pathway
Article Snippet: ELISAThe IL-6, IL-18, IL-1β and IL-8 levels in the supernatants of each coculture system (BMSCs co-cultured with Jurkat T cells and BMSCs co-cultured with RA FLS) were measured using their respective ELISA kits (R & D Systems, USA). .. Apoptosis assay5 μl fluorescein isothiocyanate-conjugated Annexin V (FITC Annexin V; BD Biosciences, USA) and 5 μl propidium iodide (PI; BD Biosciences, USA) were used to stain 1 million washed BMSCs in 200 μl 1X Annexin V binding buffer, and then incubated for 15 minutes at RT°. .. Flow cytometry Accuri C6 (BD Biosciences, San Jose, CA, USA) was used to analyze the suspended cells.

Binding Assay:

Article Title: Double-Stranded DNA Activates Glomerular Endothelial Cells and Enhances Albumin Permeability via a Toll-Like Receptor-Independent Cytosolic DNA Recognition Pathway
Article Snippet: Blots were developed with enhanced chemiluminescence (Amersham Pharmacia, Freiburg, Germany). .. B-DNA-stimulated GEnCs were washed with PBS and incubated with binding buffer containing either FITC-anti-annexin V (BD, Franklin Lakes, NJ) or propidium iodide (PI, BD) for 15 minutes at room temperature. .. In other experiments, cells were then washed with PBS and incubated with PBS containing FITC-anti-CD106 (VCAM-1) from BioLegend (San Diego, CA) or FITC-anti-CD54 (ICAM-1) from BioLegend for 45 minutes at room temperature The cells were analyzed by flow cytometry (FACS Calibur, BD) with acquisition of 30,000 events/sample.

Article Title: TL1A/TNFR2 Axis Enhances Immunoregulatory Effects of Bone Marrow Derived Mesenchymal Stem Cell by Indian Hedgehog Signaling Pathway
Article Snippet: ELISAThe IL-6, IL-18, IL-1β and IL-8 levels in the supernatants of each coculture system (BMSCs co-cultured with Jurkat T cells and BMSCs co-cultured with RA FLS) were measured using their respective ELISA kits (R & D Systems, USA). .. Apoptosis assay5 μl fluorescein isothiocyanate-conjugated Annexin V (FITC Annexin V; BD Biosciences, USA) and 5 μl propidium iodide (PI; BD Biosciences, USA) were used to stain 1 million washed BMSCs in 200 μl 1X Annexin V binding buffer, and then incubated for 15 minutes at RT°. .. Flow cytometry Accuri C6 (BD Biosciences, San Jose, CA, USA) was used to analyze the suspended cells.

Labeling:

Article Title: Antioxidant and Cytotoxic Activity of Hydroethanolic Extract from Jacaranda decurrens Leaves
Article Snippet: Cells were plated on 96-well plates (105 cells/mL) and grown in medium containing 10% FBS in the absence or presence of E-Jds (1–1000 µg/mL) for 24 h. Then, K562 cells were washed with PBS and resuspended in a buffer for annexin labeling (0.01 M HEPES (pH 7.4), 0.14 M NaCl and 2.5 mM CaCl2 ). .. Cell suspensions were labeled with annexin-FITC and propidium iodide (PI; Becton Dickinson, USA), according to the manufacturer's instructions. .. Cells were incubated at room temperature for 15 min and analyzed by flow cytometry on a FACSCalibur cytometer (Becton Dickinson, USA) using the CellQuest software (Becton Dickinson, USA).

Staining:

Article Title: TL1A/TNFR2 Axis Enhances Immunoregulatory Effects of Bone Marrow Derived Mesenchymal Stem Cell by Indian Hedgehog Signaling Pathway
Article Snippet: ELISAThe IL-6, IL-18, IL-1β and IL-8 levels in the supernatants of each coculture system (BMSCs co-cultured with Jurkat T cells and BMSCs co-cultured with RA FLS) were measured using their respective ELISA kits (R & D Systems, USA). .. Apoptosis assay5 μl fluorescein isothiocyanate-conjugated Annexin V (FITC Annexin V; BD Biosciences, USA) and 5 μl propidium iodide (PI; BD Biosciences, USA) were used to stain 1 million washed BMSCs in 200 μl 1X Annexin V binding buffer, and then incubated for 15 minutes at RT°. .. Flow cytometry Accuri C6 (BD Biosciences, San Jose, CA, USA) was used to analyze the suspended cells.

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    Becton Dickinson propidium iodide
    Quantification of viable olfactory ensheathing cells after culture harvest. (A, B) Unstained cells used as controls; <t>propidium</t> iodide staining showing 2% positivity, indicating 98% viable cells.
    Propidium Iodide, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson annexin v fitc propidium iodide pi
    The role of interaction between HAUSP and ANXA1 following UV in Jurkat cells. ( a , b ) Endogenous binding of HAUSP and ANXA1 is confirmed by endogenous immunoprecipitation analysis. Jurkat cell lysates were immunoprecipitated with an anti-HAUSP or an anti-ANXA1 antibody. Then, western blotting was performed to detect ANXA1 or HAUSP expression using the respective antibodies. ( c ) Jurkat cells, time dependently incubated with 30 mJ/cm 2 UV treatment, were lysed and immunoprecipitated with an anti-HAUSP antibody. Then, ANXA1 levels were detected by western blotting using an anti-ANXA1 antibody. ( d ) HAUSP-depleted (HAUSP KD) and control shRNA transduced (Control) Jurkat cells were generated as shown in Figure 4c . Depletion of HAUSP compared with normal Jurkat cells was detected by western blotting using an anti-HAUSP antibody. ( e ) Normal, control shRNA transduced (Control), and HAUSP-depleted Jurkat cells (HAUSP KD) were exposed to UV followed by incubation for 4 h. Then, cell lysates were immunoprecipitated with an anti-HAUSP antibody. Subsequent western blotting was performed with indicated antibodies. ( f ) Cells were stained with Annexin <t>V-FITC/PI</t> for verifying the apoptotic or necrotic cell ratio. ( g ) Jurkat, control, and HAUSP KD Jurkat cells were exposed to UV and incubated for 3 h. Then, cell supernatants were harvested and placed into the lower chamber. Subsequently, THP-1 monocytes cultured in serum-free media were placed into the upper chamber and incubated with respective cell supernatants in addition to Ac2-26 containing media. The transmigration ratio was detected as described in Materials and Methods . Each value represents the mean (±S.D.) of a representative of three independent experiments, and an asterisk mark (*) means statistical significance
    Annexin V Fitc Propidium Iodide Pi, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson annexin v propidium iodide pi apoptosis detection kit i
    The effect of CDH17 downregulation on cell growth in vitro . MKN28 cells without any treatment and non-targeted RNAi vector-mediated lenti-shCDH17-neg cells were used as controls. (A) Cell migration. (B) Cell invasion. (C) <t>Annexin</t> V and <t>propidium</t> iodide double staining detected <t>apoptosis.</t> (D) Colony formation. (E) Cell proliferation. Knockdown of CDH17 caused a significant growth inhibition of gastric cancer cells as revealed by different assays. (F) Pro-caspase-3 and cleaved caspase-3 were examined by western blotting. * P
    Annexin V Propidium Iodide Pi Apoptosis Detection Kit I, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Erianin induces T47D cells cycle arrest at G2/M phase. A. Erianin induces T47D cells cycle arrest. Cells were treated with graded concentrations of erianin (0 to 160 nM) for 48 and 72 hours. <t>Propidium</t> iodide staining was done to determine the DNA content.
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    Image Search Results


    Quantification of viable olfactory ensheathing cells after culture harvest. (A, B) Unstained cells used as controls; propidium iodide staining showing 2% positivity, indicating 98% viable cells.

    Journal: Asian Spine Journal

    Article Title: Functional Recovery Following the Transplantation of Olfactory Ensheathing Cells in Rat Spinal Cord Injury Model

    doi: 10.31616/asj.2018.12.6.998

    Figure Lengend Snippet: Quantification of viable olfactory ensheathing cells after culture harvest. (A, B) Unstained cells used as controls; propidium iodide staining showing 2% positivity, indicating 98% viable cells.

    Article Snippet: These cells were stained for propidium iodide (PI; BD Bioscience, San Diego, CA, USA), a red fluorescent DNA counterstain.

    Techniques: Staining

    The role of interaction between HAUSP and ANXA1 following UV in Jurkat cells. ( a , b ) Endogenous binding of HAUSP and ANXA1 is confirmed by endogenous immunoprecipitation analysis. Jurkat cell lysates were immunoprecipitated with an anti-HAUSP or an anti-ANXA1 antibody. Then, western blotting was performed to detect ANXA1 or HAUSP expression using the respective antibodies. ( c ) Jurkat cells, time dependently incubated with 30 mJ/cm 2 UV treatment, were lysed and immunoprecipitated with an anti-HAUSP antibody. Then, ANXA1 levels were detected by western blotting using an anti-ANXA1 antibody. ( d ) HAUSP-depleted (HAUSP KD) and control shRNA transduced (Control) Jurkat cells were generated as shown in Figure 4c . Depletion of HAUSP compared with normal Jurkat cells was detected by western blotting using an anti-HAUSP antibody. ( e ) Normal, control shRNA transduced (Control), and HAUSP-depleted Jurkat cells (HAUSP KD) were exposed to UV followed by incubation for 4 h. Then, cell lysates were immunoprecipitated with an anti-HAUSP antibody. Subsequent western blotting was performed with indicated antibodies. ( f ) Cells were stained with Annexin V-FITC/PI for verifying the apoptotic or necrotic cell ratio. ( g ) Jurkat, control, and HAUSP KD Jurkat cells were exposed to UV and incubated for 3 h. Then, cell supernatants were harvested and placed into the lower chamber. Subsequently, THP-1 monocytes cultured in serum-free media were placed into the upper chamber and incubated with respective cell supernatants in addition to Ac2-26 containing media. The transmigration ratio was detected as described in Materials and Methods . Each value represents the mean (±S.D.) of a representative of three independent experiments, and an asterisk mark (*) means statistical significance

    Journal: Cell Death & Disease

    Article Title: Annexin-1 regulated by HAUSP is essential for UV-induced damage response

    doi: 10.1038/cddis.2015.32

    Figure Lengend Snippet: The role of interaction between HAUSP and ANXA1 following UV in Jurkat cells. ( a , b ) Endogenous binding of HAUSP and ANXA1 is confirmed by endogenous immunoprecipitation analysis. Jurkat cell lysates were immunoprecipitated with an anti-HAUSP or an anti-ANXA1 antibody. Then, western blotting was performed to detect ANXA1 or HAUSP expression using the respective antibodies. ( c ) Jurkat cells, time dependently incubated with 30 mJ/cm 2 UV treatment, were lysed and immunoprecipitated with an anti-HAUSP antibody. Then, ANXA1 levels were detected by western blotting using an anti-ANXA1 antibody. ( d ) HAUSP-depleted (HAUSP KD) and control shRNA transduced (Control) Jurkat cells were generated as shown in Figure 4c . Depletion of HAUSP compared with normal Jurkat cells was detected by western blotting using an anti-HAUSP antibody. ( e ) Normal, control shRNA transduced (Control), and HAUSP-depleted Jurkat cells (HAUSP KD) were exposed to UV followed by incubation for 4 h. Then, cell lysates were immunoprecipitated with an anti-HAUSP antibody. Subsequent western blotting was performed with indicated antibodies. ( f ) Cells were stained with Annexin V-FITC/PI for verifying the apoptotic or necrotic cell ratio. ( g ) Jurkat, control, and HAUSP KD Jurkat cells were exposed to UV and incubated for 3 h. Then, cell supernatants were harvested and placed into the lower chamber. Subsequently, THP-1 monocytes cultured in serum-free media were placed into the upper chamber and incubated with respective cell supernatants in addition to Ac2-26 containing media. The transmigration ratio was detected as described in Materials and Methods . Each value represents the mean (±S.D.) of a representative of three independent experiments, and an asterisk mark (*) means statistical significance

    Article Snippet: Apoptosis was determined by staining with Annexin V-FITC/propidium iodide (PI) (BD BioScience) double staining according to the manufacturer's instructions.

    Techniques: Binding Assay, Immunoprecipitation, Western Blot, Expressing, Incubation, shRNA, Generated, Staining, Cell Culture, Transmigration Assay

    The effect of CDH17 downregulation on cell growth in vitro . MKN28 cells without any treatment and non-targeted RNAi vector-mediated lenti-shCDH17-neg cells were used as controls. (A) Cell migration. (B) Cell invasion. (C) Annexin V and propidium iodide double staining detected apoptosis. (D) Colony formation. (E) Cell proliferation. Knockdown of CDH17 caused a significant growth inhibition of gastric cancer cells as revealed by different assays. (F) Pro-caspase-3 and cleaved caspase-3 were examined by western blotting. * P

    Journal: International Journal of Oncology

    Article Title: Inhibition of CDH17 gene expression via RNA interference reduces proliferation and apoptosis of human MKN28 gastric cancer cells

    doi: 10.3892/ijo.2016.3783

    Figure Lengend Snippet: The effect of CDH17 downregulation on cell growth in vitro . MKN28 cells without any treatment and non-targeted RNAi vector-mediated lenti-shCDH17-neg cells were used as controls. (A) Cell migration. (B) Cell invasion. (C) Annexin V and propidium iodide double staining detected apoptosis. (D) Colony formation. (E) Cell proliferation. Knockdown of CDH17 caused a significant growth inhibition of gastric cancer cells as revealed by different assays. (F) Pro-caspase-3 and cleaved caspase-3 were examined by western blotting. * P

    Article Snippet: Flow cytometry for the determination of MKN28 cell apoptosis An Annexin V/propidium iodide (PI) apoptosis detection kit I (BD Biosciences) was used to measure apoptosis.

    Techniques: In Vitro, Plasmid Preparation, Migration, Double Staining, Inhibition, Western Blot

    Erianin induces T47D cells cycle arrest at G2/M phase. A. Erianin induces T47D cells cycle arrest. Cells were treated with graded concentrations of erianin (0 to 160 nM) for 48 and 72 hours. Propidium iodide staining was done to determine the DNA content.

    Journal: American Journal of Translational Research

    Article Title: Erianin inhibits the proliferation of T47D cells by inhibiting cell cycles, inducing apoptosis and suppressing migration

    doi:

    Figure Lengend Snippet: Erianin induces T47D cells cycle arrest at G2/M phase. A. Erianin induces T47D cells cycle arrest. Cells were treated with graded concentrations of erianin (0 to 160 nM) for 48 and 72 hours. Propidium iodide staining was done to determine the DNA content.

    Article Snippet: Briefly, the cells were harvested, washed twice with PBS, and fixed at 4°C for 1 h with 70% ethanol, and then stained with a propidium iodide (PI) solution (containing RNase) at 4°C for 30 min. At least 20,000 cells were analyzed by Becton Dickinson FACScan cytoflurometer (Mansfield, MA, USA).

    Techniques: Staining