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Promega promega s pgl3 promoter vector
Promega S Pgl3 Promoter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/promega s pgl3 promoter vector/product/Promega
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
promega s pgl3 promoter vector - by Bioz Stars, 2020-07
85/100 stars

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Related Articles

Clone Assay:

Article Title: Identification of an intronic cis-acting element in the human dopamine transporter gene
Article Snippet: .. To identify which regions of the 121-bp displayed the inhibitory activity, we broke down the 121-bp into four subfragments, I–IV ( ) and analyzed their individual regulatory activity on the SV40 promoter after cloning by replacing the 33 bp Hin dIII/ Nco I fragment of the Promega’s pGL3-Promoter Vector ( ). .. Subfragment I had 36 bp; II, 41 bp; III, 38 bp and IV, 22 bp with some overlapping between subfragments to reduce the possibility of losing TF binding activity ( ).

Activity Assay:

Article Title: Identification of an intronic cis-acting element in the human dopamine transporter gene
Article Snippet: .. To identify which regions of the 121-bp displayed the inhibitory activity, we broke down the 121-bp into four subfragments, I–IV ( ) and analyzed their individual regulatory activity on the SV40 promoter after cloning by replacing the 33 bp Hin dIII/ Nco I fragment of the Promega’s pGL3-Promoter Vector ( ). .. Subfragment I had 36 bp; II, 41 bp; III, 38 bp and IV, 22 bp with some overlapping between subfragments to reduce the possibility of losing TF binding activity ( ).

Plasmid Preparation:

Article Title: Identification of an intronic cis-acting element in the human dopamine transporter gene
Article Snippet: .. A 121 bp fragment was broken down into four subfragments and each subfragment was prepared by oligonucleotide (oligo) annealing to be boarded in Hin dIII and Nco I ends, followed by insertion into the Hin dIII/ Nco I sites of Promega’s pGL3-Promoter Vector, generating four SV40 subfragment plasmids pGL3-SV40-I, pGL3-SV40-II, pGL3-SV40-III and pGL3-SV40-IV. .. To replace the SV40 promoter in the subfragment plasmids with hDAT promoter, a 5,330 bp Bsa AI/ Eco RV hDAT fragment was isolated from the pGL3-hDAT7.9kb construct by Bsa AI/ Hin dIII digestion ( Hin dIII located in the reporter vector), inserted into the Sma I/ Hin dIII sites of the subfragment plasmids, generating four hDAT subfragment plasmids pGL3-hDAT5.3kb-I, pGL3-hDAT5.3kb-II, pGL3-hDAT5.3kb-III and pGL3-hDAT5.3kb-IV.

Article Title: Identification of an intronic cis-acting element in the human dopamine transporter gene
Article Snippet: .. To identify which regions of the 121-bp displayed the inhibitory activity, we broke down the 121-bp into four subfragments, I–IV ( ) and analyzed their individual regulatory activity on the SV40 promoter after cloning by replacing the 33 bp Hin dIII/ Nco I fragment of the Promega’s pGL3-Promoter Vector ( ). .. Subfragment I had 36 bp; II, 41 bp; III, 38 bp and IV, 22 bp with some overlapping between subfragments to reduce the possibility of losing TF binding activity ( ).

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  • 92
    Promega pgl3 promoter vector
    Conservative ARE analysis and luciferase reporter analysis of the LAMC1 gene. ( A ) Comparative transcription factor binding site analysis of the LAMC1 gene across different mammalian species shows highly-conserved NRF2 AREs. ( B ) Left, siNRF2-C27 and siGFP-C5 cells subjected to ChIP analysis with anti-Nrf2. Right, the relative ability of NRF2 to bind to the ARE site (value of NRF2 in siGFP-C5 cells set at 1; PCR reactions were not saturated; results are from at least 3 separate experiments; HO-1 served as positive control; GAPDH served as negative control). ( C ) tBHQ increased 151-bp LAMC1 promoter (sequence at left; red indicates ARE sequences)–luciferase activity in MCF7 cells. The plasmid <t>pGL3-LAMC1-151bp</t> was transfected into MCF7 cells in combination with pRL-TK for 24 h. Dual luciferase activity was measured after treatment with tBHQ (20 μM) for 6 h. Control, DMSO treatment for the same plasmid was set at 1 (mean ± SD, n=3; **p
    Pgl3 Promoter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 595 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgl3 promoter vector/product/Promega
    Average 92 stars, based on 595 article reviews
    Price from $9.99 to $1999.99
    pgl3 promoter vector - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Promega xhoi digested pgl3 promoter vector
    Conservative ARE analysis and luciferase reporter analysis of the LAMC1 gene. ( A ) Comparative transcription factor binding site analysis of the LAMC1 gene across different mammalian species shows highly-conserved NRF2 AREs. ( B ) Left, siNRF2-C27 and siGFP-C5 cells subjected to ChIP analysis with anti-Nrf2. Right, the relative ability of NRF2 to bind to the ARE site (value of NRF2 in siGFP-C5 cells set at 1; PCR reactions were not saturated; results are from at least 3 separate experiments; HO-1 served as positive control; GAPDH served as negative control). ( C ) tBHQ increased 151-bp LAMC1 promoter (sequence at left; red indicates ARE sequences)–luciferase activity in MCF7 cells. The plasmid <t>pGL3-LAMC1-151bp</t> was transfected into MCF7 cells in combination with pRL-TK for 24 h. Dual luciferase activity was measured after treatment with tBHQ (20 μM) for 6 h. Control, DMSO treatment for the same plasmid was set at 1 (mean ± SD, n=3; **p
    Xhoi Digested Pgl3 Promoter Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xhoi digested pgl3 promoter vector/product/Promega
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xhoi digested pgl3 promoter vector - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Conservative ARE analysis and luciferase reporter analysis of the LAMC1 gene. ( A ) Comparative transcription factor binding site analysis of the LAMC1 gene across different mammalian species shows highly-conserved NRF2 AREs. ( B ) Left, siNRF2-C27 and siGFP-C5 cells subjected to ChIP analysis with anti-Nrf2. Right, the relative ability of NRF2 to bind to the ARE site (value of NRF2 in siGFP-C5 cells set at 1; PCR reactions were not saturated; results are from at least 3 separate experiments; HO-1 served as positive control; GAPDH served as negative control). ( C ) tBHQ increased 151-bp LAMC1 promoter (sequence at left; red indicates ARE sequences)–luciferase activity in MCF7 cells. The plasmid pGL3-LAMC1-151bp was transfected into MCF7 cells in combination with pRL-TK for 24 h. Dual luciferase activity was measured after treatment with tBHQ (20 μM) for 6 h. Control, DMSO treatment for the same plasmid was set at 1 (mean ± SD, n=3; **p

    Journal: Aging (Albany NY)

    Article Title: Genome-wide global identification of NRF2 binding sites in A549 non-small cell lung cancer cells by ChIP-Seq reveals NRF2 regulation of genes involved in focal adhesion pathways

    doi: 10.18632/aging.102590

    Figure Lengend Snippet: Conservative ARE analysis and luciferase reporter analysis of the LAMC1 gene. ( A ) Comparative transcription factor binding site analysis of the LAMC1 gene across different mammalian species shows highly-conserved NRF2 AREs. ( B ) Left, siNRF2-C27 and siGFP-C5 cells subjected to ChIP analysis with anti-Nrf2. Right, the relative ability of NRF2 to bind to the ARE site (value of NRF2 in siGFP-C5 cells set at 1; PCR reactions were not saturated; results are from at least 3 separate experiments; HO-1 served as positive control; GAPDH served as negative control). ( C ) tBHQ increased 151-bp LAMC1 promoter (sequence at left; red indicates ARE sequences)–luciferase activity in MCF7 cells. The plasmid pGL3-LAMC1-151bp was transfected into MCF7 cells in combination with pRL-TK for 24 h. Dual luciferase activity was measured after treatment with tBHQ (20 μM) for 6 h. Control, DMSO treatment for the same plasmid was set at 1 (mean ± SD, n=3; **p

    Article Snippet: The amplified DNA fragment was subsequently cloned into the pGL3-promoter vector (Promega, Shanghai, China) via the Xho I and Kpn I restriction sites and verified by DNA sequencing.

    Techniques: Luciferase, Binding Assay, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Positive Control, Negative Control, Sequencing, Activity Assay, Plasmid Preparation, Transfection