procomplex crystal screen (Qiagen)

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Qiagen procomplex crystal screen
Procomplex Crystal Screen, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/procomplex/classics+crystal+screens/pmc11728977-339-10-13
Average 90 stars, based on 1 article reviews

procomplex crystal screen - by Bioz Stars, 2026-09

90/100 stars

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Crystallization Assay:

Article Title: Nanobody Mediated Crystallization of an Archeal Mechanosensitive Channel
Article Snippet: Before crystallization setup, the IMP/nanobody samples were centrifuged at 20,400 × g in a cooled bench top centrifuge for 10 min. Crystallization trials were performed using a mosquito (TTP LabTech) crystallization robot with a total drop volume of 300 nl (at 3 different protein/precipitant ratios: 1:1, 1:2 and 2:1). .. The two sparse matrix screens, ProComplex and JCSG+ (Qiagen) were used for initial crystallization trials at 20 °C. .. Crystal growth was followed over 21 days using the Rock Imager software (Formulatrix).

Article Title: Crystal Structure of human Karyopherin ?2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2
Article Snippet: .. Purified Kapβ2• Nab2 PY-NLS complex was screened against MCSG1-4 (Microlytic North America, USA) and ProComplex (Qiagen, USA) crystallization screens via sitting drop vapor diffusion at 20°C (0.4 μL protein + 0.4 μL reservoir solution) using a Phoenix (Art Robins, USA) liquid handling system. ..

Article Title: Structural basis for the interaction of a human small heat shock protein with the 14-3-3 universal signaling regulator
Article Snippet: Titration curves were buffer-corrected and then fitted using MicroCal Origin 9.0 software by either a classical quadratic equation (in the absence of phosphopeptide) or, in a competition assay, by a cubic equation according to the method by Wang ( Wang, 1995 ), to estimate the apparent binding constant. .. Initial search for crystallization conditions was done using commercial screens including PACT, Procomplex (Qiagen), Wizard I+II (Emerald BioStructures), Index (Hampton Research) and JCSG+ (Molecular Dimensions). ..

Article Title: Chimeric 14-3-3 proteins for unravelling interactions with intrinsically disordered partners
Article Snippet: .. The 14-3-3 chimeras were subjected to crystallization screening immediately after purification by SEC using commercial screens including PACT, Procomplex (Qiagen), Index, Crystal Screen (Hampton Research) and JCSG+ (Molecular Dimensions). ..

Article Title: Stepwise Evolution Improves Identification of Diverse Peptides Binding to a Protein Target
Article Snippet: .. Peptide sequence EWVHPQFEQKAK GNSFDDWLASKG AFPDYLAEYHGG RDPAPAWAHGGG GyGlanvdessG Gdlwqheatwkkq GGwhdeatwkpG NQpWQ Data collection Peptide-protein complex formation Peptide stock: 50mM in water Incubate 3h at RT in 1:2 molar access Peptide stock: 10mM in water Incubate 3h at RT in 1:2 molar access Peptide stock: 50mM in Hepes 7.0 Incubate 3h at RT in 1:3 molar access Peptide stock: 50mM in water Incubate 3h at RT in 1:2 molar access Peptide stock: 50mM in DMSO Incubate 4h at RT in 1:3 molar access Peptide stock: 10mM in water Incubate 3h at RT in 1:3 molar access Peptide stock: 10mM in water Incubate 3h at RT in 1:3 molar access Peptide stock: 5mM in water Incubate ON at RT in 1:3 molar ratio Crystallization condition Procomplex (Qiagen) 0.1M Magnesium acetate 0.1M Sodium cacodylate pH 6.5 15% PEG6000 0.1M Potassium chloride 0.1M HEPES pH 7.5 15% PEG6000 0.1M Magnesium chloride 0.1M Sodium citrate pH 5 15% PEG4000 0.2M Ammonium acetate 0.1M Sodium acetate pH 4 15% PEG4000 0.1M Sodium citrate pH 5.6 20% PEG4000 20% Isopropanol 0.1M Sodium citrate pH 4.5 20% PEG4000 0.1M Magnesium acetate 0.1M MOPS pH 7.5 12% PEG8000 0.1M Sodium citrate pH 5.5 15% PEG6000 Peptide occupancy All 8 sites All 8 sites All 4 sites 3 sites occupied/1 with unidentified mol. ..

Article Title: Structural insights into regulation of CNNM-TRPM7 divalent cation uptake by the small GTPase ARL15
Article Snippet: Results were analyzed using ORIGIN software (MicroCal) and fitted to a binding model with a single set of identical sites. .. Initial crystallization conditions were identified utilizing hanging drop vapor diffusion with the Classics II and ProComplex screens (QIAGEN). .. The best crystals were obtained by equilibrating a 0.6 μl drop at 12 mg/ml of the complex of ARL15 (32–197) and CNNM2 CBS domain (429–584) with close to a stoichiometric amount of GppNHp, non- hydrolyzable GTP analog (Abcam) in HPLC buffer mixed with 0.6 μl of reservoir solution containing 0.2 M sodium chloride, 0.1 M Tris pH 8.5, 25% (wt/vol) PEG3350.

Article Title: A Proline-Rich Element in the Type III Secretion Protein FlhB Contributes to Flagellar Biogenesis in the Beta- and Gamma-Proteobacteria
Article Snippet: .. For crystallization, JCSG core solutions I-IV and ProComplex (Qiagen) were used, together with two-well crystallization plates, suited for the sitting drop method (Swiss MRC). ..

Purification:

Diffusion-based Assay:

Sequencing:

Whisker Assay:

Article Title: Complex assembly, crystallization and preliminary X-ray crystallographic analysis of the human Rod–Zwilch–ZW10 (RZZ) complex
Article Snippet: .. Composition of reservoir solution/screening kit , The JSCG Core IIV, ProComplex, Anions, Cations, Cryo, PEGs, PEGs II and AmSO 4 Suites (Qiagen) , Buffer, pH, salt and glycerol were varied systematically around condition 69 of the The ProComplex Suite as well as other conditions identified by initial screening of RZZ complex devoid of His 6 tag and/or dephosphorylated , Additive Screen (Hampton Research) was applied to various conditions obtained after initial optimization , In drops with various conditions obtained after initial optimization but with precipitant concentrations reduced to 75% of the original condition. Seed-stock preparation: crystals from a similar condition were typically mixed with 100200l reservoir solution and smashed by adding a glass bead and vortexing followed by serial streaking with a cat whisker , Based on various conditions obtained after initial optimization, performed as in Dong et al. (2007 ). Trypsin and elastase dilutions from 1:100 to 1:20000 . ..

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