pro spc  (Vector Laboratories)


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    Vector Laboratories pro spc
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro spc/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro spc - by Bioz Stars, 2024-06
    96/100 stars

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    1) Product Images from "Timing and cell specificity of senescence drives postnatal lung development and injury"

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    Journal: Nature Communications

    doi: 10.1038/s41467-023-35985-4

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Figure Legend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Techniques Used: Immunofluorescence

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).
    Figure Legend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Techniques Used: Isolation, Immunofluorescence, Luminex, Injection, Cell Culture, Staining

    pro spc  (Vector Laboratories)


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    Vector Laboratories pro spc
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro spc/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro spc - by Bioz Stars, 2024-06
    96/100 stars

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    1) Product Images from "Timing and cell specificity of senescence drives postnatal lung development and injury"

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    Journal: Nature Communications

    doi: 10.1038/s41467-023-35985-4

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
    Figure Legend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Techniques Used: Immunofluorescence

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).
    Figure Legend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Techniques Used: Isolation, Immunofluorescence, Luminex, Injection, Cell Culture, Staining

    pro spc  (Vector Laboratories)


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    Vector Laboratories pro spc
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro spc/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
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    pro spc - by Bioz Stars, 2024-06
    96/100 stars

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    antibody against pro surfactant protein c spc  (Vector Laboratories)


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    Vector Laboratories antibody against pro surfactant protein c spc
    Antibody Against Pro Surfactant Protein C Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti pro spc antibody  (Vector Laboratories)


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    Vector Laboratories anti pro spc antibody
    LPS-induced Cxcl5 expression in mice lungs. a Lung sections were prepared from both mice that were and those that were not subjected to LPS administration in the indicated PLCε genotype. Sections were subjected to Cxcl5 and <t>pro-SPC</t> immunostaining ( n = 3 mice/group). Scale bar, 100 μm. b BALF was collected in 24 h after i.t. administration of LPS or vehicle, the expression levels of Cxcl5 in BALF were measured by ELISA. n = 9 in the LPS-treated group, and n = 6 in the vehicle-treated group. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes
    Anti Pro Spc Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pro spc antibody/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
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    96/100 stars

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    1) Product Images from "Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells"

    Article Title: Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells

    Journal: Respiratory Research

    doi: 10.1186/s12931-019-0975-4

    LPS-induced Cxcl5 expression in mice lungs. a Lung sections were prepared from both mice that were and those that were not subjected to LPS administration in the indicated PLCε genotype. Sections were subjected to Cxcl5 and pro-SPC immunostaining ( n = 3 mice/group). Scale bar, 100 μm. b BALF was collected in 24 h after i.t. administration of LPS or vehicle, the expression levels of Cxcl5 in BALF were measured by ELISA. n = 9 in the LPS-treated group, and n = 6 in the vehicle-treated group. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes
    Figure Legend Snippet: LPS-induced Cxcl5 expression in mice lungs. a Lung sections were prepared from both mice that were and those that were not subjected to LPS administration in the indicated PLCε genotype. Sections were subjected to Cxcl5 and pro-SPC immunostaining ( n = 3 mice/group). Scale bar, 100 μm. b BALF was collected in 24 h after i.t. administration of LPS or vehicle, the expression levels of Cxcl5 in BALF were measured by ELISA. n = 9 in the LPS-treated group, and n = 6 in the vehicle-treated group. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes

    Techniques Used: Expressing, Immunostaining, Enzyme-linked Immunosorbent Assay

    anti pro spc  (Vector Laboratories)


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    Vector Laboratories anti pro spc
    LAT1 is expressed in type I AECs (A, arrows), with co-localization <t>with</t> <t>AQP5</t> (B, arrow, brown labeling) to the alveolar septum. Co-localization with <t>pro-SPC</t> was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Anti Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pro spc/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pro spc - by Bioz Stars, 2024-06
    96/100 stars

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    1) Product Images from "Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung"

    Article Title: Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung

    Journal: Pediatric pulmonology

    doi: 10.1002/ppul.23402

    LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Figure Legend Snippet: LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.

    Techniques Used: Labeling, Incubation

    pro spc  (Vector Laboratories)


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    Vector Laboratories pro spc
    LAT1 is expressed in type I AECs (A, arrows), with co-localization <t>with</t> <t>AQP5</t> (B, arrow, brown labeling) to the alveolar septum. Co-localization with <t>pro-SPC</t> was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro spc/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro spc - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung"

    Article Title: Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung

    Journal: Pediatric pulmonology

    doi: 10.1002/ppul.23402

    LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Figure Legend Snippet: LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.

    Techniques Used: Labeling, Incubation

    anti pro spc  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
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    Vector Laboratories anti pro spc
    LAT1 is expressed in type I AECs (A, arrows), with co-localization <t>with</t> <t>AQP5</t> (B, arrow, brown labeling) to the alveolar septum. Co-localization with <t>pro-SPC</t> was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Anti Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pro spc/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti pro spc - by Bioz Stars, 2024-06
    96/100 stars

    Images

    1) Product Images from "Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung"

    Article Title: Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung

    Journal: Pediatric pulmonology

    doi: 10.1002/ppul.23402

    LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Figure Legend Snippet: LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.

    Techniques Used: Labeling, Incubation

    pro spc  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
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    Vector Laboratories pro spc
    LAT1 is expressed in type I AECs (A, arrows), with co-localization <t>with</t> <t>AQP5</t> (B, arrow, brown labeling) to the alveolar septum. Co-localization with <t>pro-SPC</t> was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro spc/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pro spc - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung"

    Article Title: Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung

    Journal: Pediatric pulmonology

    doi: 10.1002/ppul.23402

    LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Figure Legend Snippet: LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.

    Techniques Used: Labeling, Incubation

    pro spc  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
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    Vector Laboratories pro spc
    Disruption of E-cadherin in Scgb1a1-expressing cells impairs Clara cell lineage differentiation and induces ectopic expression of <t>pro</t> <t>SpC</t> in conducting airways. (A) Representative dual immunofluorescence staining of lung sections for tubulin (red) and Scgb1a1 (green). Individual bronchioles are encircled by dashed lines. Nuclei were counterstained with DAPI (blue). (B) Morphometric analysis of ciliated cells. Results are presented as means ± SEM for each group. *P < .05. BM, basement membrane. (C) Gene expression as a function of age. Total lung RNA from control and induced mice was assayed for expression of Scgb1a1, cytochrome P450-2F2, FoxJ1, SpC, and AQP-5 by quantitative RT-PCR, and average ddCT values are presented ± SEM. NB, new born. (D) Immunohistochemistry shows ectopic expression of the alveolar type II cell marker pro Sftpc (red arrow) in bronchiolar epithelium of an induced mouse. Type II pneumocytes (red asterisks) served as an internal control for pro Sftpc staining (brown). Hematoxylin (blue) was used as a counterstain. (E) The percentage of terminal bronchioles containing zero, one, and two cells with ectopic pro Sftpc expression is shown. At least 220 bronchioles were counted from six DTR mice for each group. Results are presented as means ± SEM for each group. *P < .05.
    Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "E-cadherin Controls Bronchiolar Progenitor Cells and Onset of Preneoplastic Lesions in Mice"

    Article Title: E-cadherin Controls Bronchiolar Progenitor Cells and Onset of Preneoplastic Lesions in Mice

    Journal: Neoplasia (New York, N.Y.)

    doi:

    Disruption of E-cadherin in Scgb1a1-expressing cells impairs Clara cell lineage differentiation and induces ectopic expression of pro SpC in conducting airways. (A) Representative dual immunofluorescence staining of lung sections for tubulin (red) and Scgb1a1 (green). Individual bronchioles are encircled by dashed lines. Nuclei were counterstained with DAPI (blue). (B) Morphometric analysis of ciliated cells. Results are presented as means ± SEM for each group. *P < .05. BM, basement membrane. (C) Gene expression as a function of age. Total lung RNA from control and induced mice was assayed for expression of Scgb1a1, cytochrome P450-2F2, FoxJ1, SpC, and AQP-5 by quantitative RT-PCR, and average ddCT values are presented ± SEM. NB, new born. (D) Immunohistochemistry shows ectopic expression of the alveolar type II cell marker pro Sftpc (red arrow) in bronchiolar epithelium of an induced mouse. Type II pneumocytes (red asterisks) served as an internal control for pro Sftpc staining (brown). Hematoxylin (blue) was used as a counterstain. (E) The percentage of terminal bronchioles containing zero, one, and two cells with ectopic pro Sftpc expression is shown. At least 220 bronchioles were counted from six DTR mice for each group. Results are presented as means ± SEM for each group. *P < .05.
    Figure Legend Snippet: Disruption of E-cadherin in Scgb1a1-expressing cells impairs Clara cell lineage differentiation and induces ectopic expression of pro SpC in conducting airways. (A) Representative dual immunofluorescence staining of lung sections for tubulin (red) and Scgb1a1 (green). Individual bronchioles are encircled by dashed lines. Nuclei were counterstained with DAPI (blue). (B) Morphometric analysis of ciliated cells. Results are presented as means ± SEM for each group. *P < .05. BM, basement membrane. (C) Gene expression as a function of age. Total lung RNA from control and induced mice was assayed for expression of Scgb1a1, cytochrome P450-2F2, FoxJ1, SpC, and AQP-5 by quantitative RT-PCR, and average ddCT values are presented ± SEM. NB, new born. (D) Immunohistochemistry shows ectopic expression of the alveolar type II cell marker pro Sftpc (red arrow) in bronchiolar epithelium of an induced mouse. Type II pneumocytes (red asterisks) served as an internal control for pro Sftpc staining (brown). Hematoxylin (blue) was used as a counterstain. (E) The percentage of terminal bronchioles containing zero, one, and two cells with ectopic pro Sftpc expression is shown. At least 220 bronchioles were counted from six DTR mice for each group. Results are presented as means ± SEM for each group. *P < .05.

    Techniques Used: Expressing, Immunofluorescence, Staining, Quantitative RT-PCR, Immunohistochemistry, Marker

    pro spc  (Vector Laboratories)


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    Structured Review

    Vector Laboratories pro spc
    Disruption of E-cadherin in Scgb1a1-expressing cells impairs Clara cell lineage differentiation and induces ectopic expression of <t>pro</t> <t>SpC</t> in conducting airways. (A) Representative dual immunofluorescence staining of lung sections for tubulin (red) and Scgb1a1 (green). Individual bronchioles are encircled by dashed lines. Nuclei were counterstained with DAPI (blue). (B) Morphometric analysis of ciliated cells. Results are presented as means ± SEM for each group. *P < .05. BM, basement membrane. (C) Gene expression as a function of age. Total lung RNA from control and induced mice was assayed for expression of Scgb1a1, cytochrome P450-2F2, FoxJ1, SpC, and AQP-5 by quantitative RT-PCR, and average ddCT values are presented ± SEM. NB, new born. (D) Immunohistochemistry shows ectopic expression of the alveolar type II cell marker pro Sftpc (red arrow) in bronchiolar epithelium of an induced mouse. Type II pneumocytes (red asterisks) served as an internal control for pro Sftpc staining (brown). Hematoxylin (blue) was used as a counterstain. (E) The percentage of terminal bronchioles containing zero, one, and two cells with ectopic pro Sftpc expression is shown. At least 220 bronchioles were counted from six DTR mice for each group. Results are presented as means ± SEM for each group. *P < .05.
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    Images

    1) Product Images from "E-cadherin Controls Bronchiolar Progenitor Cells and Onset of Preneoplastic Lesions in Mice 1 2 "

    Article Title: E-cadherin Controls Bronchiolar Progenitor Cells and Onset of Preneoplastic Lesions in Mice 1 2

    Journal: Neoplasia (New York, N.Y.)

    doi:

    Disruption of E-cadherin in Scgb1a1-expressing cells impairs Clara cell lineage differentiation and induces ectopic expression of pro SpC in conducting airways. (A) Representative dual immunofluorescence staining of lung sections for tubulin (red) and Scgb1a1 (green). Individual bronchioles are encircled by dashed lines. Nuclei were counterstained with DAPI (blue). (B) Morphometric analysis of ciliated cells. Results are presented as means ± SEM for each group. *P < .05. BM, basement membrane. (C) Gene expression as a function of age. Total lung RNA from control and induced mice was assayed for expression of Scgb1a1, cytochrome P450-2F2, FoxJ1, SpC, and AQP-5 by quantitative RT-PCR, and average ddCT values are presented ± SEM. NB, new born. (D) Immunohistochemistry shows ectopic expression of the alveolar type II cell marker pro Sftpc (red arrow) in bronchiolar epithelium of an induced mouse. Type II pneumocytes (red asterisks) served as an internal control for pro Sftpc staining (brown). Hematoxylin (blue) was used as a counterstain. (E) The percentage of terminal bronchioles containing zero, one, and two cells with ectopic pro Sftpc expression is shown. At least 220 bronchioles were counted from six DTR mice for each group. Results are presented as means ± SEM for each group. *P < .05.
    Figure Legend Snippet: Disruption of E-cadherin in Scgb1a1-expressing cells impairs Clara cell lineage differentiation and induces ectopic expression of pro SpC in conducting airways. (A) Representative dual immunofluorescence staining of lung sections for tubulin (red) and Scgb1a1 (green). Individual bronchioles are encircled by dashed lines. Nuclei were counterstained with DAPI (blue). (B) Morphometric analysis of ciliated cells. Results are presented as means ± SEM for each group. *P < .05. BM, basement membrane. (C) Gene expression as a function of age. Total lung RNA from control and induced mice was assayed for expression of Scgb1a1, cytochrome P450-2F2, FoxJ1, SpC, and AQP-5 by quantitative RT-PCR, and average ddCT values are presented ± SEM. NB, new born. (D) Immunohistochemistry shows ectopic expression of the alveolar type II cell marker pro Sftpc (red arrow) in bronchiolar epithelium of an induced mouse. Type II pneumocytes (red asterisks) served as an internal control for pro Sftpc staining (brown). Hematoxylin (blue) was used as a counterstain. (E) The percentage of terminal bronchioles containing zero, one, and two cells with ectopic pro Sftpc expression is shown. At least 220 bronchioles were counted from six DTR mice for each group. Results are presented as means ± SEM for each group. *P < .05.

    Techniques Used: Expressing, Immunofluorescence, Staining, Quantitative RT-PCR, Immunohistochemistry, Marker

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    Vector Laboratories pro spc
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
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    Vector Laboratories antibody against pro surfactant protein c spc
    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear <t>lamin</t> <t>b1</t> with <t>pro-SPC</t> (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.
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    Vector Laboratories anti pro spc antibody
    LPS-induced Cxcl5 expression in mice lungs. a Lung sections were prepared from both mice that were and those that were not subjected to LPS administration in the indicated PLCε genotype. Sections were subjected to Cxcl5 and <t>pro-SPC</t> immunostaining ( n = 3 mice/group). Scale bar, 100 μm. b BALF was collected in 24 h after i.t. administration of LPS or vehicle, the expression levels of Cxcl5 in BALF were measured by ELISA. n = 9 in the LPS-treated group, and n = 6 in the vehicle-treated group. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes
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    Vector Laboratories anti pro spc
    LAT1 is expressed in type I AECs (A, arrows), with co-localization <t>with</t> <t>AQP5</t> (B, arrow, brown labeling) to the alveolar septum. Co-localization with <t>pro-SPC</t> was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.
    Anti Pro Spc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Journal: Nature Communications

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    doi: 10.1038/s41467-023-35985-4

    Figure Lengend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. a , b Immunofluorescence was performed to determine co-localization of p21 and loss of nuclear lamin b1 with pro-SPC (SPC) in the lung. Cells lacking nuclear LB1 but positive for SPC ( c ), or p21 + /SPC + ( d ) were counted and normalized to total numbers of nuclei. Arrows denote nuclear loss of lamin b1 in SPC + ( a ), or p21 + /SPC + ( b ) cells . Bar size: 50 µm. Data are expressed as mean ± SEM. N = 5–7 mice per group. Source data are provided as a Source Data file. t -test was used for comparison. ** P < 0.01 vs air group.

    Article Snippet: Sections were de-paraffinized and subjected to heat-mediated antigen retrieval in a citrate buffer solution (Vector Labs), then incubated with antibodies against lamin b1, p21, Ki67, γ-H2AX, 53BP1, 8-oxo-DG, phosphor-p53, Hopx, vWF, vimentin, pro-SPC, Pdgfra, Sox9, and F4/80 overnight at 4 °C.

    Techniques: Immunofluorescence

    C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Journal: Nature Communications

    Article Title: Timing and cell specificity of senescence drives postnatal lung development and injury

    doi: 10.1038/s41467-023-35985-4

    Figure Lengend Snippet: C57BL/6 J neonatal mice (<12 h old) were exposed to air or hyperoxia (95% O 2 ) for 3 days followed by air recovery until pnd7. Senescent type II cells were isolated from these mice through the sorting of pro-SPC and C12FDG positive cells. a Immunofluorescence of pro-SPC was performed in these cells after 24 h of culture. Arrows denote pro-SPC positive cells. Bar size: 50 µm. b Luminex assay was performed to evaluate the levels of SASP factors in the supernatants. c , d C57BL/6 J neonatal mice were intranasally injected with 20 μl of culture medium with or without dilution (1-part medium:1-part saline) of cultured cells at pnd7 and pnd10. H&E and vWF staining was performed to measure mean linear intercept (Lm), radial alveolar count (RAC) and number of blood vessels in the lung at pnd14. Bar size: 100 µm. Data are expressed as mean ± SEM. N = 5–6 mice per group. Source data are provided as a Source Data file. One-way ANOVA followed by Tukey post-test was used for multiple comparisons ( c , d ), while t -test was used in panel ( b ). * P < 0.05, ** P < 0.01, *** P < 0.001 vs air ( b ) or vehicle ( c , d ).

    Article Snippet: Sections were de-paraffinized and subjected to heat-mediated antigen retrieval in a citrate buffer solution (Vector Labs), then incubated with antibodies against lamin b1, p21, Ki67, γ-H2AX, 53BP1, 8-oxo-DG, phosphor-p53, Hopx, vWF, vimentin, pro-SPC, Pdgfra, Sox9, and F4/80 overnight at 4 °C.

    Techniques: Isolation, Immunofluorescence, Luminex, Injection, Cell Culture, Staining

    LPS-induced Cxcl5 expression in mice lungs. a Lung sections were prepared from both mice that were and those that were not subjected to LPS administration in the indicated PLCε genotype. Sections were subjected to Cxcl5 and pro-SPC immunostaining ( n = 3 mice/group). Scale bar, 100 μm. b BALF was collected in 24 h after i.t. administration of LPS or vehicle, the expression levels of Cxcl5 in BALF were measured by ELISA. n = 9 in the LPS-treated group, and n = 6 in the vehicle-treated group. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes

    Journal: Respiratory Research

    Article Title: Phospholipase Cε plays a crucial role in neutrophilic inflammation accompanying acute lung injury through augmentation of CXC chemokine production from alveolar epithelial cells

    doi: 10.1186/s12931-019-0975-4

    Figure Lengend Snippet: LPS-induced Cxcl5 expression in mice lungs. a Lung sections were prepared from both mice that were and those that were not subjected to LPS administration in the indicated PLCε genotype. Sections were subjected to Cxcl5 and pro-SPC immunostaining ( n = 3 mice/group). Scale bar, 100 μm. b BALF was collected in 24 h after i.t. administration of LPS or vehicle, the expression levels of Cxcl5 in BALF were measured by ELISA. n = 9 in the LPS-treated group, and n = 6 in the vehicle-treated group. *, p < 0.05 between control and LPS administration, #, p < 0.05 between PLCε genotypes

    Article Snippet: The paraffin-embedded sections were also subjected to immunohistochemical staining with the anti-Cxcl5 antibody (15 μg/ml) and the anti-pro-SPC antibody (1 μg/ml) along with the peroxidase polymer anti-rabbit IgG reagents by using the Polymer method with ImmPRESS reagent (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Expressing, Immunostaining, Enzyme-linked Immunosorbent Assay

    LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.

    Journal: Pediatric pulmonology

    Article Title: Bronchopulmonary Dysplasia Impairs L-Type Amino Acid Transporter-1 Expression in Human and Baboon Lung

    doi: 10.1002/ppul.23402

    Figure Lengend Snippet: LAT1 is expressed in type I AECs (A, arrows), with co-localization with AQP5 (B, arrow, brown labeling) to the alveolar septum. Co-localization with pro-SPC was seen in type II AECs (C, arrow). There was no labeling in sections incubated without primary antibody (D). Bar = 50 μm.

    Article Snippet: Cellular sites of expression were determined in sections that were co-incubated with anti-AQP5 or anti-pro-SPC, then detected with ImmPRESS anti-rabbit horseradish peroxidase (Vector Laboratories, Burlingame, CA).

    Techniques: Labeling, Incubation