prk5 ha gst ragd s77l gdp  (Addgene inc)

 
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    Addgene inc prk5 ha gst ragd s77l gdp

    Prk5 Ha Gst Ragd S77l Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "An mTORC1-to-CDK1 Switch Maintains Autophagy Suppression during Mitosis"

    Article Title: An mTORC1-to-CDK1 Switch Maintains Autophagy Suppression during Mitosis

    Journal: Molecular Cell

    doi: 10.1016/j.molcel.2019.10.016


    Figure Legend Snippet:

    Techniques Used: Western Blot, Recombinant, Software

    prk5 ha gst ragd gdp  (Addgene inc)

     
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    Addgene inc prk5 ha gst ragd gdp

    Prk5 Ha Gst Ragd Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prk5 ha gst ragd gdp/product/Addgene inc
    Average 92 stars, based on 1 article reviews
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    1) Product Images from "The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage"

    Article Title: The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage

    Journal: eLife

    doi: 10.7554/eLife.40856


    Figure Legend Snippet:

    Techniques Used: Transfection, Construct, Plasmid Preparation, Transduction

    prk5 ha gst ragd gdp  (Addgene inc)

     
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    Addgene inc prk5 ha gst ragd gdp
    Prk5 Ha Gst Ragd Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prk5 ha gst ragd gdp  (Addgene inc)

     
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    Addgene inc prk5 ha gst ragd gdp
    Prk5 Ha Gst Ragd Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    prk5 ha gst ragd gdp  (Addgene inc)

     
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    Addgene inc prk5 ha gst ragd gdp
    Rag GTPases and mTORC1 regulate the function of MITF. (A) Multisequence alignment of the first 30 amino acids of TFEB with MITF isoforms 1, 2, 3, and 7. The green arrows indicate the residues identified as essential for the interaction between TFEB and Rag proteins. Blue letters are to indicate conserved amino acid homology between the different proteins. (B–D) ARPE-19 cells were infected with adenovirus expressing MITF1-FLAG. 16 h later, cells were incubated with DMSO or 250 nM Torin-1 for 1 h and analyzed by immunoblotting (C) or immunofluorescence (B and D) with anti-FLAG antibodies. The region within the dotted box is magnified in the insets. Bars: (B and D, main images) 10 µm; (D, insets) 5 µm. (E) ARPE-19 cells were incubated with 250 nM Torin-1 for 1 h. Cells were then lysed and analyzed by immunoblotting with antibodies against MITF. (F) HEK-293T cells were incubated in medium containing DMSO or Torin-1 for 1 h. Cells were lysed, and nuclei and membrane plus cytosol fractions were obtained by low speed centrifugation. Proteins from the different fractions were subjected to immunoblotting with antibodies against MITF, Lamp1, and Histone H3. (G) ARPE-19 cells expressing active (RagB GTP <t>/RagD</t> <t>GDP</t> ) or inactive (RagB GDP /RagD GTP ) Rag heterodimers were immunoprecipitated with the anti-FLAG antibody and immunoblotted with antibodies against FLAG and <t>GST</t> (used to detect MITF and Rag proteins, respectively). (H) HEK-293T cells expressing active (RagB GTP /RagD GDP ) or inactive (RagB GDP /RagD GTP ) Rag heterodimers were pulled down using glutathione–Sepharose beads and immunoblotted with antibodies against GST and MITF (used to detect Rag proteins and endogenous MITF, respectively). IP, immunoprecipitation.
    Prk5 Ha Gst Ragd Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Rag GTPases mediate amino acid–dependent recruitment of TFEB and MITF to lysosomes"

    Article Title: Rag GTPases mediate amino acid–dependent recruitment of TFEB and MITF to lysosomes

    Journal: The Journal of Cell Biology

    doi: 10.1083/jcb.201209135

    Rag GTPases and mTORC1 regulate the function of MITF. (A) Multisequence alignment of the first 30 amino acids of TFEB with MITF isoforms 1, 2, 3, and 7. The green arrows indicate the residues identified as essential for the interaction between TFEB and Rag proteins. Blue letters are to indicate conserved amino acid homology between the different proteins. (B–D) ARPE-19 cells were infected with adenovirus expressing MITF1-FLAG. 16 h later, cells were incubated with DMSO or 250 nM Torin-1 for 1 h and analyzed by immunoblotting (C) or immunofluorescence (B and D) with anti-FLAG antibodies. The region within the dotted box is magnified in the insets. Bars: (B and D, main images) 10 µm; (D, insets) 5 µm. (E) ARPE-19 cells were incubated with 250 nM Torin-1 for 1 h. Cells were then lysed and analyzed by immunoblotting with antibodies against MITF. (F) HEK-293T cells were incubated in medium containing DMSO or Torin-1 for 1 h. Cells were lysed, and nuclei and membrane plus cytosol fractions were obtained by low speed centrifugation. Proteins from the different fractions were subjected to immunoblotting with antibodies against MITF, Lamp1, and Histone H3. (G) ARPE-19 cells expressing active (RagB GTP /RagD GDP ) or inactive (RagB GDP /RagD GTP ) Rag heterodimers were immunoprecipitated with the anti-FLAG antibody and immunoblotted with antibodies against FLAG and GST (used to detect MITF and Rag proteins, respectively). (H) HEK-293T cells expressing active (RagB GTP /RagD GDP ) or inactive (RagB GDP /RagD GTP ) Rag heterodimers were pulled down using glutathione–Sepharose beads and immunoblotted with antibodies against GST and MITF (used to detect Rag proteins and endogenous MITF, respectively). IP, immunoprecipitation.
    Figure Legend Snippet: Rag GTPases and mTORC1 regulate the function of MITF. (A) Multisequence alignment of the first 30 amino acids of TFEB with MITF isoforms 1, 2, 3, and 7. The green arrows indicate the residues identified as essential for the interaction between TFEB and Rag proteins. Blue letters are to indicate conserved amino acid homology between the different proteins. (B–D) ARPE-19 cells were infected with adenovirus expressing MITF1-FLAG. 16 h later, cells were incubated with DMSO or 250 nM Torin-1 for 1 h and analyzed by immunoblotting (C) or immunofluorescence (B and D) with anti-FLAG antibodies. The region within the dotted box is magnified in the insets. Bars: (B and D, main images) 10 µm; (D, insets) 5 µm. (E) ARPE-19 cells were incubated with 250 nM Torin-1 for 1 h. Cells were then lysed and analyzed by immunoblotting with antibodies against MITF. (F) HEK-293T cells were incubated in medium containing DMSO or Torin-1 for 1 h. Cells were lysed, and nuclei and membrane plus cytosol fractions were obtained by low speed centrifugation. Proteins from the different fractions were subjected to immunoblotting with antibodies against MITF, Lamp1, and Histone H3. (G) ARPE-19 cells expressing active (RagB GTP /RagD GDP ) or inactive (RagB GDP /RagD GTP ) Rag heterodimers were immunoprecipitated with the anti-FLAG antibody and immunoblotted with antibodies against FLAG and GST (used to detect MITF and Rag proteins, respectively). (H) HEK-293T cells expressing active (RagB GTP /RagD GDP ) or inactive (RagB GDP /RagD GTP ) Rag heterodimers were pulled down using glutathione–Sepharose beads and immunoblotted with antibodies against GST and MITF (used to detect Rag proteins and endogenous MITF, respectively). IP, immunoprecipitation.

    Techniques Used: Infection, Expressing, Incubation, Western Blot, Immunofluorescence, Centrifugation, Immunoprecipitation

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    Addgene inc prk5 ha gst ragd s77l gdp

    Prk5 Ha Gst Ragd S77l Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prk5 ha gst ragd s77l gdp/product/Addgene inc
    Average 92 stars, based on 1 article reviews
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    prk5 ha gst ragd gdp  (Addgene inc)
    92
    Addgene inc prk5 ha gst ragd gdp

    Prk5 Ha Gst Ragd Gdp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prk5 ha gst ragd gdp/product/Addgene inc
    Average 92 stars, based on 1 article reviews
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    Journal: Molecular Cell

    Article Title: An mTORC1-to-CDK1 Switch Maintains Autophagy Suppression during Mitosis

    doi: 10.1016/j.molcel.2019.10.016

    Figure Lengend Snippet:

    Article Snippet: pRK5-HA GST RagD S77L (GDP) , ( ) , Addgene #19308.

    Techniques: Western Blot, Recombinant, Software

    Journal: eLife

    Article Title: The transcription factors TFE3 and TFEB amplify p53 dependent transcriptional programs in response to DNA damage

    doi: 10.7554/eLife.40856

    Figure Lengend Snippet:

    Article Snippet: The following constructs were obtained from Addgene: plasmid 19303, pRK5-HA-GST-RagB GTP and plasmid 19308, pRK5-HA-GST-RagD GDP ( ).

    Techniques: Transfection, Construct, Plasmid Preparation, Transduction