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InvivoGen primocin
Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and <t>Primocin.</t> The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.
Primocin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primocin/product/InvivoGen
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primocin - by Bioz Stars, 2022-01
99/100 stars

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1) Product Images from "Chamber-specific differences in human cardiac fibroblast proliferation and responsiveness toward simvastatin"

Article Title: Chamber-specific differences in human cardiac fibroblast proliferation and responsiveness toward simvastatin

Journal: American Journal of Physiology - Cell Physiology

doi: 10.1152/ajpcell.00056.2016

Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and Primocin. The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.
Figure Legend Snippet: Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and Primocin. The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.

Techniques Used: Viability Assay, Staining, Ethidium Homodimer Assay, Microscopy

2) Product Images from "Chamber-specific differences in human cardiac fibroblast proliferation and responsiveness toward simvastatin"

Article Title: Chamber-specific differences in human cardiac fibroblast proliferation and responsiveness toward simvastatin

Journal: American Journal of Physiology - Cell Physiology

doi: 10.1152/ajpcell.00056.2016

Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and Primocin. The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.
Figure Legend Snippet: Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and Primocin. The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.

Techniques Used: Viability Assay, Staining, Ethidium Homodimer Assay, Microscopy

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    InvivoGen primocin
    Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and <t>Primocin.</t> The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.
    Primocin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primocin/product/InvivoGen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primocin - by Bioz Stars, 2022-01
    99/100 stars
      Buy from Supplier

    86
    InvivoGen 1x primocin
    Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and <t>Primocin.</t> The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.
    1x Primocin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x primocin/product/InvivoGen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    1x primocin - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

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    Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and Primocin. The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: Chamber-specific differences in human cardiac fibroblast proliferation and responsiveness toward simvastatin

    doi: 10.1152/ajpcell.00056.2016

    Figure Lengend Snippet: Cardiac fibroblasts were grown to confluence in media containing 2.5% FBS and Primocin. The cells were treated with or without simvastatin (1 μM) for 48 h and assessed for cytotoxicity using cell viability assay by staining the cells with calcein AM and ethidium homodimer (EthD-1) for 30 min at 37°C ( A–H ). Cytotoxicity was determined by fluorescent microscopy, and cell counts of live and dead cells were expressed as a percentage of the total cells counted. Calcein AM-positive or EthD-1-positive cells in atrial fibroblasts without ( A and B ) or with 1 μM simvastatin ( C and D ), and in ventricular fibroblasts without simvastatin ( E and F ) and with 1 μM simvastatin ( G and H ) are shown, respectively. I : percentage of calcein AM-positive and EthD-1-positive cells ( n = 3, means ± SE; P = NS, not significant). Assessment of cytotoxicity also was determined by lactate dehydrogenase (LDH) release from atrial ( J ) and ventricular ( K ) fibroblasts after 24-h exposure to different doses of simvastatin (0–20 μM). Vent, ventricular.

    Article Snippet: To prevent the fibroblasts from spontaneously differentiating to myofibroblasts, studies were carried out in low-serum (1.25%, 2.50%, or 3.75% FBS) medium after initial plating in HCF media containing 5% FBS + penicillin-streptomycin or Primocin (InvivoGen, San Diego, CA) for 24 h.

    Techniques: Viability Assay, Staining, Ethidium Homodimer Assay, Microscopy