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TaKaRa primescript rt reagent kit
Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 7592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primescript rt reagent kit/product/TaKaRa
Average 90 stars, based on 7592 article reviews
Price from $9.99 to $1999.99
primescript rt reagent kit - by Bioz Stars, 2020-01
90/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma
Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) RNeasy mini kit (Qiagen, Hilden, Germany) was used to extract total RNA from 40 mg tumor or normal tissue samples and cells and next cDNA was synthesized using PrimeScript RT reagent kit (Takara, Tokyo, Japan). .. For real-time qPCR, we used the 7500 Real-Time PCR System using SYBR® Green (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

RNA Extraction:

Article Title: Duplication and transcriptional divergence of three Kunitz protease inhibitor genes that modulate insect and pathogen defenses in tea plant (Camellia sinensis)
Article Snippet: Paragraph title: Total RNA extraction and qRT-PCR analysis ... The PrimeScript RT Reagent Kit (cat 6110A, Takara) was used to synthesize first-strand cDNA from total RNA.

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

Amplification:

Article Title: Duplication and transcriptional divergence of three Kunitz protease inhibitor genes that modulate insect and pathogen defenses in tea plant (Camellia sinensis)
Article Snippet: The PrimeScript RT Reagent Kit (cat 6110A, Takara) was used to synthesize first-strand cDNA from total RNA. .. The amplification efficiencies of all genes used in this study ranged from 95 to 105% .

Synthesized:

Article Title: USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma
Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) RNeasy mini kit (Qiagen, Hilden, Germany) was used to extract total RNA from 40 mg tumor or normal tissue samples and cells and next cDNA was synthesized using PrimeScript RT reagent kit (Takara, Tokyo, Japan). .. For real-time qPCR, we used the 7500 Real-Time PCR System using SYBR® Green (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

Size-exclusion Chromatography:

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

Quantitative RT-PCR:

Article Title: Duplication and transcriptional divergence of three Kunitz protease inhibitor genes that modulate insect and pathogen defenses in tea plant (Camellia sinensis)
Article Snippet: Paragraph title: Total RNA extraction and qRT-PCR analysis ... The PrimeScript RT Reagent Kit (cat 6110A, Takara) was used to synthesize first-strand cDNA from total RNA.

Article Title: USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma
Article Snippet: .. Quantitative real time polymerase chain reaction (qRT-PCR) RNeasy mini kit (Qiagen, Hilden, Germany) was used to extract total RNA from 40 mg tumor or normal tissue samples and cells and next cDNA was synthesized using PrimeScript RT reagent kit (Takara, Tokyo, Japan). .. For real-time qPCR, we used the 7500 Real-Time PCR System using SYBR® Green (Thermo Fisher Scientific, Waltham, MA, USA).

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

SYBR Green Assay:

Article Title: USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma
Article Snippet: Quantitative real time polymerase chain reaction (qRT-PCR) RNeasy mini kit (Qiagen, Hilden, Germany) was used to extract total RNA from 40 mg tumor or normal tissue samples and cells and next cDNA was synthesized using PrimeScript RT reagent kit (Takara, Tokyo, Japan). .. For real-time qPCR, we used the 7500 Real-Time PCR System using SYBR® Green (Thermo Fisher Scientific, Waltham, MA, USA).

Cell Culture:

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

Expressing:

Article Title: USP21 promotes cell proliferation and metastasis through suppressing EZH2 ubiquitination in bladder carcinoma
Article Snippet: Quantitative real time polymerase chain reaction (qRT-PCR) RNeasy mini kit (Qiagen, Hilden, Germany) was used to extract total RNA from 40 mg tumor or normal tissue samples and cells and next cDNA was synthesized using PrimeScript RT reagent kit (Takara, Tokyo, Japan). .. The relative expression of mRNA was normalized against β-actin.

Polymerase Chain Reaction:

Article Title: Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2
Article Snippet: .. RNA extraction and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis Total RNA from the cultured cells was extracted using TRIzol (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, and cDNA was synthesized using the PrimeScript RT reagent kit (Takara Biotechnology Co., Ltd., Dalian, China; cat. no. DRR037A). qPCR was performed using SYBR Premix Ex Taq II (Takara Biotechnology Co., Ltd.; cat. no. DRR820A) and measured in a LightCycler 480 system (Roche Diagnostics, Basel, Switzerland). .. The thermocycling procedure was as follows: Initial denaturation at 95°C for 5 min, followed by 39 cycles at 95°C for 5 sec, and at 60°C for 30 sec. GAPDH was used as the endogenous control and relative quantification was evaluated using the 2−ΔΔCq method ( ).

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    TaKaRa primescript rt reagent kit
    Primescript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 7592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primescript rt reagent kit/product/TaKaRa
    Average 90 stars, based on 7592 article reviews
    Price from $9.99 to $1999.99
    primescript rt reagent kit - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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