primer sequences  (TaKaRa)


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  • 86
    Name:
    ApoPrimer Set
    Description:

    Catalog Number:
    6623
    Price:
    None
    Category:
    PCR
    Size:
    20 Rxns
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    TaKaRa primer sequences

    https://www.bioz.com/result/primer sequences/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primer sequences - by Bioz Stars, 2021-07
    86/100 stars

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    Article Snippet: The primers of bax (412-bp product), bcl-2 (380-bp product), and β-actin (275-bp product) were used ApoPrimer Set (Bcl-2 family, Takara, Tokyo, Japan).

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    Article Title: Double inhibition of XIAP and Bcl-2 axis is beneficial for retrieving sensitivity of renal cell cancer to apoptosis
    Article Snippet: To ensure appropriate first-strand cDNA synthesis, the β -actin gene RT–PCR was performed as described by . .. Bcl-2 family members were amplified using an ApoPrimer Set (Takara Bio, Shiga, Japan) according to the manufacturer's instructions. ..

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  • 98
    TaKaRa human mtdna monitoring primer set
    False-positive detection of bisulfite-resistant cytosines (brCs) in the negative control amplicons (NCAs) of <t>mtDNA</t> by bisulfite pyrosequencing. BrCs were detected in gel-purified or unpurified, bisulfite-converted PCR amplicons by pyrosequencing for 15 CpG sites: 5 in human <t>ND1,</t> 5 in human CYTB, 2 in mouse ND1, and 3 in mouse CYTB. The p -value was calculated using paired t-test (two-tails). The lines show mean ± 95% Confidence Intervals.
    Human Mtdna Monitoring Primer Set, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human mtdna monitoring primer set/product/TaKaRa
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human mtdna monitoring primer set - by Bioz Stars, 2021-07
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    96
    TaKaRa n22 region
    TTV circular genome (thin line) with ORFs 1 and 2 indicated. Anchored PCR extension clones extending upstream and downstream from the <t>N22</t> clone sequence (gray box) described by Nishizawa et al. ) are indicated by the arrows inside the circular genome. The inverse PCR product (ud) that overlaps the anchored PCR products is also shown. The 113-nt sequence identified in the GH1 isolate is indicated by the hatched box. Position 1 is indicated by the arrow.
    N22 Region, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n22 region/product/TaKaRa
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    95
    TaKaRa random primed cdna
    Tissue expression of hMSH6 <t>mRNA.</t> A human multiple-tissue Northern blot (CLONTECH) was probed with the 32 P-labeled complete <t>cDNA</t> clone of hMSH6 according to the manufacturer’s protocol. Colon refers to mucosal lining; p.b. leukocyte refers to peripheral blood leukocyte. The hMSH6 transcript is indicated by the arrow and corresponds to ≈4.5 kb. The amount of RNA loaded in each lane was adjusted to comparable levels as judged spectrophotometrically and by the levels of actin present (CLONTECH).
    Random Primed Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random primed cdna/product/TaKaRa
    Average 95 stars, based on 1 article reviews
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    False-positive detection of bisulfite-resistant cytosines (brCs) in the negative control amplicons (NCAs) of mtDNA by bisulfite pyrosequencing. BrCs were detected in gel-purified or unpurified, bisulfite-converted PCR amplicons by pyrosequencing for 15 CpG sites: 5 in human ND1, 5 in human CYTB, 2 in mouse ND1, and 3 in mouse CYTB. The p -value was calculated using paired t-test (two-tails). The lines show mean ± 95% Confidence Intervals.

    Journal: PLoS ONE

    Article Title: Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

    doi: 10.1371/journal.pone.0192722

    Figure Lengend Snippet: False-positive detection of bisulfite-resistant cytosines (brCs) in the negative control amplicons (NCAs) of mtDNA by bisulfite pyrosequencing. BrCs were detected in gel-purified or unpurified, bisulfite-converted PCR amplicons by pyrosequencing for 15 CpG sites: 5 in human ND1, 5 in human CYTB, 2 in mouse ND1, and 3 in mouse CYTB. The p -value was calculated using paired t-test (two-tails). The lines show mean ± 95% Confidence Intervals.

    Article Snippet: Purity of human mtDNA was evaluated by qPCR detection of mtDNA markers (MT-ND1 and MT-ND5) and nuclear DNA markers (SLCO2B1 and SERPINA1) using Human mtDNA Monitoring Primer Set (Cat# 7246; Takara Bio).

    Techniques: Negative Control, Purification, Polymerase Chain Reaction

    Detection of bisulfite-resistant cytosines in purified, linearized human mtDNA by bisulfite pyrosequencing using converted template-selective (A9515) and unselective (hND1) sequencing primers. Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The A9515 sequencing primer, which was highly selective to bisulfite-converted DNA, interrogated three CpG sites (CpG #3–5) whereas non-selective sequencing primer hND1 interrogated all these CpG sites plus two additional CpG sites (CpG #1 and 2). (B) Positive control assay was performed using in vitro partially methylated NCAs templates. High CpG methylation levels at three CpG sites (CpG #3–5) were detected using A9515 sequencing primer (CpG sites #1 and #2 were out of the assay coverage using this sequencing primer). hND1 sequencing primer detected high CpG methylation at all five CpG sites (CpG #1–5).

    Journal: PLoS ONE

    Article Title: Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

    doi: 10.1371/journal.pone.0192722

    Figure Lengend Snippet: Detection of bisulfite-resistant cytosines in purified, linearized human mtDNA by bisulfite pyrosequencing using converted template-selective (A9515) and unselective (hND1) sequencing primers. Ratios of brCs were determined by bisulfite pyrosequencing (Mean±SD, triplicated assays). (A) The A9515 sequencing primer, which was highly selective to bisulfite-converted DNA, interrogated three CpG sites (CpG #3–5) whereas non-selective sequencing primer hND1 interrogated all these CpG sites plus two additional CpG sites (CpG #1 and 2). (B) Positive control assay was performed using in vitro partially methylated NCAs templates. High CpG methylation levels at three CpG sites (CpG #3–5) were detected using A9515 sequencing primer (CpG sites #1 and #2 were out of the assay coverage using this sequencing primer). hND1 sequencing primer detected high CpG methylation at all five CpG sites (CpG #1–5).

    Article Snippet: Purity of human mtDNA was evaluated by qPCR detection of mtDNA markers (MT-ND1 and MT-ND5) and nuclear DNA markers (SLCO2B1 and SERPINA1) using Human mtDNA Monitoring Primer Set (Cat# 7246; Takara Bio).

    Techniques: Purification, Sequencing, Positive Control Assay, In Vitro, Methylation, CpG Methylation Assay

    Enrichment of human and mouse mtDNA over nuclear DNA. Ratios of copy numbers of human (A) or mouse (B) mtDNA and nuclear DNA were determined by qPCR of mtDNA and nuclear DNA marker genes (Mean±SD, triplicated assays). Numbers show fold enrichment compared to total DNA.

    Journal: PLoS ONE

    Article Title: Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

    doi: 10.1371/journal.pone.0192722

    Figure Lengend Snippet: Enrichment of human and mouse mtDNA over nuclear DNA. Ratios of copy numbers of human (A) or mouse (B) mtDNA and nuclear DNA were determined by qPCR of mtDNA and nuclear DNA marker genes (Mean±SD, triplicated assays). Numbers show fold enrichment compared to total DNA.

    Article Snippet: Purity of human mtDNA was evaluated by qPCR detection of mtDNA markers (MT-ND1 and MT-ND5) and nuclear DNA markers (SLCO2B1 and SERPINA1) using Human mtDNA Monitoring Primer Set (Cat# 7246; Takara Bio).

    Techniques: Real-time Polymerase Chain Reaction, Marker

    Shotgun bisulfite sequencing of human iPSC mtDNA. (A) Ratios of copy numbers of human mtDNA and nuclear DNA were determined by qPCR of mtDNA and nuclear DNA marker genes (Mean±SD, triplicated assays). Numbers show fold enrichment compared to total DNA. (B) CpG methylation of mtDNA-encoded genes (box plot; boxes and whiskers indicate quartiles and minimum/maximum values, horizontal lines in boxes indicate median). Numbers at the top indicate p -values of 2-tailed t-test against unmethylated lambda DNA. (C, D) Cytosine methylation of mtDNA (C) and unmethylated lambda DNA (D). Percentage of cytosine methylation in the CpG and non-CpG contexts is shown with red and blue dots, respectively. Deep sequencing read coverage at cytosines is shown with green dots. In panel (C), locations of mtDNA-encoded genes and D-loop are indicated at the top, where positions of tRNA genes are shown with vertical bars without gene names.

    Journal: PLoS ONE

    Article Title: Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

    doi: 10.1371/journal.pone.0192722

    Figure Lengend Snippet: Shotgun bisulfite sequencing of human iPSC mtDNA. (A) Ratios of copy numbers of human mtDNA and nuclear DNA were determined by qPCR of mtDNA and nuclear DNA marker genes (Mean±SD, triplicated assays). Numbers show fold enrichment compared to total DNA. (B) CpG methylation of mtDNA-encoded genes (box plot; boxes and whiskers indicate quartiles and minimum/maximum values, horizontal lines in boxes indicate median). Numbers at the top indicate p -values of 2-tailed t-test against unmethylated lambda DNA. (C, D) Cytosine methylation of mtDNA (C) and unmethylated lambda DNA (D). Percentage of cytosine methylation in the CpG and non-CpG contexts is shown with red and blue dots, respectively. Deep sequencing read coverage at cytosines is shown with green dots. In panel (C), locations of mtDNA-encoded genes and D-loop are indicated at the top, where positions of tRNA genes are shown with vertical bars without gene names.

    Article Snippet: Purity of human mtDNA was evaluated by qPCR detection of mtDNA markers (MT-ND1 and MT-ND5) and nuclear DNA markers (SLCO2B1 and SERPINA1) using Human mtDNA Monitoring Primer Set (Cat# 7246; Takara Bio).

    Techniques: Methylation Sequencing, Real-time Polymerase Chain Reaction, Marker, CpG Methylation Assay, Lambda DNA Preparation, Methylation, Sequencing

    Enrichment of human and mouse mtDNA over nuclear DNA. Ratios of copy numbers of human (A) or mouse (B) mtDNA and nuclear DNA were determined by qPCR of mtDNA and nuclear DNA marker genes (Mean±SD, triplicated assays). Numbers show fold enrichment compared to total DNA.

    Journal: PLoS ONE

    Article Title: Technical adequacy of bisulfite sequencing and pyrosequencing for detection of mitochondrial DNA methylation: Sources and avoidance of false-positive detection

    doi: 10.1371/journal.pone.0192722

    Figure Lengend Snippet: Enrichment of human and mouse mtDNA over nuclear DNA. Ratios of copy numbers of human (A) or mouse (B) mtDNA and nuclear DNA were determined by qPCR of mtDNA and nuclear DNA marker genes (Mean±SD, triplicated assays). Numbers show fold enrichment compared to total DNA.

    Article Snippet: Purity of human mtDNA was evaluated by qPCR detection of mtDNA markers (MT-ND1 and MT-ND5) and nuclear DNA markers (SLCO2B1 and SERPINA1) using Human mtDNA Monitoring Primer Set (Cat# 7246; Takara Bio).

    Techniques: Real-time Polymerase Chain Reaction, Marker

    TTV circular genome (thin line) with ORFs 1 and 2 indicated. Anchored PCR extension clones extending upstream and downstream from the N22 clone sequence (gray box) described by Nishizawa et al. ) are indicated by the arrows inside the circular genome. The inverse PCR product (ud) that overlaps the anchored PCR products is also shown. The 113-nt sequence identified in the GH1 isolate is indicated by the hatched box. Position 1 is indicated by the arrow.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Molecular and biophysical characterization of TT virus: Evidence for a new virus family infecting humans

    doi:

    Figure Lengend Snippet: TTV circular genome (thin line) with ORFs 1 and 2 indicated. Anchored PCR extension clones extending upstream and downstream from the N22 clone sequence (gray box) described by Nishizawa et al. ) are indicated by the arrows inside the circular genome. The inverse PCR product (ud) that overlaps the anchored PCR products is also shown. The 113-nt sequence identified in the GH1 isolate is indicated by the hatched box. Position 1 is indicated by the arrow.

    Article Snippet: Furthermore, inverse PCR with primers derived from the N22 region were able to produce a 3,700-bp product encompassing nearly the entire genome, including those sequences originally believed to be at the 5′ and 3′ termini.

    Techniques: Polymerase Chain Reaction, Clone Assay, Sequencing, Inverse PCR

    Tissue expression of hMSH6 mRNA. A human multiple-tissue Northern blot (CLONTECH) was probed with the 32 P-labeled complete cDNA clone of hMSH6 according to the manufacturer’s protocol. Colon refers to mucosal lining; p.b. leukocyte refers to peripheral blood leukocyte. The hMSH6 transcript is indicated by the arrow and corresponds to ≈4.5 kb. The amount of RNA loaded in each lane was adjusted to comparable levels as judged spectrophotometrically and by the levels of actin present (CLONTECH).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6

    doi:

    Figure Lengend Snippet: Tissue expression of hMSH6 mRNA. A human multiple-tissue Northern blot (CLONTECH) was probed with the 32 P-labeled complete cDNA clone of hMSH6 according to the manufacturer’s protocol. Colon refers to mucosal lining; p.b. leukocyte refers to peripheral blood leukocyte. The hMSH6 transcript is indicated by the arrow and corresponds to ≈4.5 kb. The amount of RNA loaded in each lane was adjusted to comparable levels as judged spectrophotometrically and by the levels of actin present (CLONTECH).

    Article Snippet: Single-strand, random-primed cDNA was prepared from human testis poly(A) mRNA (CLONTECH) using a Promega cDNA synthesis kit.

    Techniques: Expressing, Northern Blot, Labeling

    Organization of hMSH6 genomic locus and sequence of the intron region flanking each MSH6 exon. Boxes containing numbers 1 through 10 indicate the individual MSH6 exons and their relative sizes. The size of each exon is given below each exon and the size of each intron is given above the region between each pair of exons. The sizes of exons 1 and 10 are the sizes of the mRNA sequence upstream and downstream of the first and last introns, respectively; these sizes were calculated from the longest MSH6 cDNA sequence available. The first 20 nucleotides of each intron sequence up to the intron-exon junction is given in uppercase letters except for the 3′ side of the last intron, where additional sequence is given. The first 10 nucleotides of each exon sequence up to the intron-exon junction is given in lowercase letters. In addition, the sequence of the 5′ and 3′ ends of the cDNA, minus the poly(A) sequence, is also given in lowercase letters. The numbers in parentheses between intron sequences are the nucleotide coordinates of the exon sequences or cDNA sequences, assuming the A of the ATG is nucleotide 1. (Additional intron sequence including the complete sequence of some introns has been determined in all cases and is available on request from M.F.K. and R.K.)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6

    doi:

    Figure Lengend Snippet: Organization of hMSH6 genomic locus and sequence of the intron region flanking each MSH6 exon. Boxes containing numbers 1 through 10 indicate the individual MSH6 exons and their relative sizes. The size of each exon is given below each exon and the size of each intron is given above the region between each pair of exons. The sizes of exons 1 and 10 are the sizes of the mRNA sequence upstream and downstream of the first and last introns, respectively; these sizes were calculated from the longest MSH6 cDNA sequence available. The first 20 nucleotides of each intron sequence up to the intron-exon junction is given in uppercase letters except for the 3′ side of the last intron, where additional sequence is given. The first 10 nucleotides of each exon sequence up to the intron-exon junction is given in lowercase letters. In addition, the sequence of the 5′ and 3′ ends of the cDNA, minus the poly(A) sequence, is also given in lowercase letters. The numbers in parentheses between intron sequences are the nucleotide coordinates of the exon sequences or cDNA sequences, assuming the A of the ATG is nucleotide 1. (Additional intron sequence including the complete sequence of some introns has been determined in all cases and is available on request from M.F.K. and R.K.)

    Article Snippet: Single-strand, random-primed cDNA was prepared from human testis poly(A) mRNA (CLONTECH) using a Promega cDNA synthesis kit.

    Techniques: Sequencing