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GenScript primer sequences
Primer Sequences, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer sequences/product/GenScript
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
primer sequences - by Bioz Stars, 2020-07
92/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
Article Snippet: .. The primer sequences were designed by PrimerBank ( ) and synthesized by GeneScript (Nanjing, China) as follows: HIF-1α, forward 5′-ACTTGGCAACCTTGGATTGGA-3′ and reverse 5′-ATCTCCGTCCCTCAACCTCT-3′ (190 bp); EGF, forward 5′-AGAGGGAGAGGATGCCACAT-3′ and reverse 5′-GGGGTGGAGTAGAGTCAAGA-3′ (206 bp); HGF, forward 5′-ACAGCTTTTTGCCTTCGAGC-3′ and reverse 5′-GCAAGAATTTGTGCCGGTGT-3′ (261 bp); VEGF, forward 5′-TCACCAAGGCCAGCACATAG-3′ and reverse 5′-GAGGCTCCAGGGCATTAGAC-3′ (202 bp); FGF2, forward 5′-TCCACCTATAATTGGTCAAAGTGGT-3′ and reverse 5′-CATCAGTTACCAGCTCCCCC-3′ (121 bp); tumor necrosis factor (TNF)-α, forward 5′-CTGGGCAGGTCTACTTTGGG-3′ and reverse 5′-CTGGAGGCCCCAGTTTGAAT-3′ (272 bp); interleukin (IL)-6, forward: 5′-TGCAATAACCACCCCTGACC-3′ and reverse 5′-GTGCCCATGCTACATTTGCC-3′ (163 bp). qPCR was performed on a StepOnePlus™ Real-time PCR System (Applied Biosystems™; Thermo Fisher Scientific, Inc.) with the following cycle: 95°C for 1 min, followed by 95°C for 15 sec, 58°C for 30 sec, and 72°C for 30 sec for 40 cycles. .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH; forward 5′-GATCCCGCTAACATCAAATG-3′ and reverse 5′-GAGGGAGTTGTCATATTTCTC-3′) expression was used as an internal control.

Size-exclusion Chromatography:

Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
Article Snippet: .. The primer sequences were designed by PrimerBank ( ) and synthesized by GeneScript (Nanjing, China) as follows: HIF-1α, forward 5′-ACTTGGCAACCTTGGATTGGA-3′ and reverse 5′-ATCTCCGTCCCTCAACCTCT-3′ (190 bp); EGF, forward 5′-AGAGGGAGAGGATGCCACAT-3′ and reverse 5′-GGGGTGGAGTAGAGTCAAGA-3′ (206 bp); HGF, forward 5′-ACAGCTTTTTGCCTTCGAGC-3′ and reverse 5′-GCAAGAATTTGTGCCGGTGT-3′ (261 bp); VEGF, forward 5′-TCACCAAGGCCAGCACATAG-3′ and reverse 5′-GAGGCTCCAGGGCATTAGAC-3′ (202 bp); FGF2, forward 5′-TCCACCTATAATTGGTCAAAGTGGT-3′ and reverse 5′-CATCAGTTACCAGCTCCCCC-3′ (121 bp); tumor necrosis factor (TNF)-α, forward 5′-CTGGGCAGGTCTACTTTGGG-3′ and reverse 5′-CTGGAGGCCCCAGTTTGAAT-3′ (272 bp); interleukin (IL)-6, forward: 5′-TGCAATAACCACCCCTGACC-3′ and reverse 5′-GTGCCCATGCTACATTTGCC-3′ (163 bp). qPCR was performed on a StepOnePlus™ Real-time PCR System (Applied Biosystems™; Thermo Fisher Scientific, Inc.) with the following cycle: 95°C for 1 min, followed by 95°C for 15 sec, 58°C for 30 sec, and 72°C for 30 sec for 40 cycles. .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH; forward 5′-GATCCCGCTAACATCAAATG-3′ and reverse 5′-GAGGGAGTTGTCATATTTCTC-3′) expression was used as an internal control.

Synthesized:

Article Title: Molecular mechanism of the role of carbamyl erythropoietin in treating diabetic retinopathy rats
Article Snippet: .. The primer sequences were synthesized by GenScript (Nanjing, China). ..

Article Title: Hypoxia-inducible factor-1α mediates the toll-like receptor 4 signaling pathway leading to anti-tumor effects in human hepatocellular carcinoma cells under hypoxic conditions
Article Snippet: .. The primer sequences were designed by PrimerBank ( ) and synthesized by GeneScript (Nanjing, China) as follows: HIF-1α, forward 5′-ACTTGGCAACCTTGGATTGGA-3′ and reverse 5′-ATCTCCGTCCCTCAACCTCT-3′ (190 bp); EGF, forward 5′-AGAGGGAGAGGATGCCACAT-3′ and reverse 5′-GGGGTGGAGTAGAGTCAAGA-3′ (206 bp); HGF, forward 5′-ACAGCTTTTTGCCTTCGAGC-3′ and reverse 5′-GCAAGAATTTGTGCCGGTGT-3′ (261 bp); VEGF, forward 5′-TCACCAAGGCCAGCACATAG-3′ and reverse 5′-GAGGCTCCAGGGCATTAGAC-3′ (202 bp); FGF2, forward 5′-TCCACCTATAATTGGTCAAAGTGGT-3′ and reverse 5′-CATCAGTTACCAGCTCCCCC-3′ (121 bp); tumor necrosis factor (TNF)-α, forward 5′-CTGGGCAGGTCTACTTTGGG-3′ and reverse 5′-CTGGAGGCCCCAGTTTGAAT-3′ (272 bp); interleukin (IL)-6, forward: 5′-TGCAATAACCACCCCTGACC-3′ and reverse 5′-GTGCCCATGCTACATTTGCC-3′ (163 bp). qPCR was performed on a StepOnePlus™ Real-time PCR System (Applied Biosystems™; Thermo Fisher Scientific, Inc.) with the following cycle: 95°C for 1 min, followed by 95°C for 15 sec, 58°C for 30 sec, and 72°C for 30 sec for 40 cycles. .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH; forward 5′-GATCCCGCTAACATCAAATG-3′ and reverse 5′-GAGGGAGTTGTCATATTTCTC-3′) expression was used as an internal control.

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    GenScript zscan21
    <t>ZSCAN21</t> expression in different brain areas from embryonic and adult rats. Different brain areas were isolated from embryonic (E17) and adult rats. The brain areas isolated were: midbrain ( MID ) or midbrain dorsal ( MD ) and midbrain ventral ( MV ), striatum
    Zscan21, supplied by GenScript, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zscan21/product/GenScript
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    zscan21 - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    GenScript pcr primer sequences
    MiR-638 directly targets <t>VEGFA</t> ( A ) Schematic representation of the 3′UTR of VEGFA mRNA containing the putative miR-638 target site. ( B ) Relative luciferase assays comparing the SK-ES-1 and RD-ES cells transfected with pGL3-mutant (VEGFA_MUT) or pGL3-wild-type (VEGFA_WT) or untreated pGL3 vector (miR-638_PC). ( C ) The mRNA levels of VEGFA were measured by Quantitative <t>RT-PCR</t> in EWS cells after transfected with miR-638 or scramble mimic, respectively. ( D ) Protein levels of VEGFA and GAPDH were detected by Western blot in EWS cells after transfected with miR-638/scramble. * P
    Pcr Primer Sequences, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer sequences/product/GenScript
    Average 91 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pcr primer sequences - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    91
    GenScript rs734553
    The polymorphism loci <t>rs734553</t> sequencing figure in 7 introns of SLC2A9 gene A:heterozygoustype (A/C); B: homozygous type (A/A)
    Rs734553, supplied by GenScript, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rs734553/product/GenScript
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rs734553 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results


    ZSCAN21 expression in different brain areas from embryonic and adult rats. Different brain areas were isolated from embryonic (E17) and adult rats. The brain areas isolated were: midbrain ( MID ) or midbrain dorsal ( MD ) and midbrain ventral ( MV ), striatum

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: ZSCAN21 expression in different brain areas from embryonic and adult rats. Different brain areas were isolated from embryonic (E17) and adult rats. The brain areas isolated were: midbrain ( MID ) or midbrain dorsal ( MD ) and midbrain ventral ( MV ), striatum

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Expressing, Isolation

    Increased transcriptional activity of the SNCA promoter following ZSCAN21 down-regulation in rat cortical cultures. A, schematic of the 5′ genomic region of human SNCA . Exons are depicted as closed boxes and introns as lines . The canonical 5′

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: Increased transcriptional activity of the SNCA promoter following ZSCAN21 down-regulation in rat cortical cultures. A, schematic of the 5′ genomic region of human SNCA . Exons are depicted as closed boxes and introns as lines . The canonical 5′

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Activity Assay

    AAV-mediated silencing of ZSCAN21 did not alter SNCA levels in the DG of the hippocampus in adult rat. A, efficiency of neuronal transduction of the AAVs ( AAV/shZSCAN21/2 and AAV/shscrambled ) in 4-month-old rat brains. Co-localization of NeuN ( red ) and

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: AAV-mediated silencing of ZSCAN21 did not alter SNCA levels in the DG of the hippocampus in adult rat. A, efficiency of neuronal transduction of the AAVs ( AAV/shZSCAN21/2 and AAV/shscrambled ) in 4-month-old rat brains. Co-localization of NeuN ( red ) and

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Transduction

    ZSCAN21 binds in the intron 1 regulatory region. A, schematic of the rat SNCA gene; exons are depicted as boxes and introns as lines . The specific location in the rat SNCA gene of the amplified area containing the two ZSCAN21 putative binding sites (ZSCAN21)

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: ZSCAN21 binds in the intron 1 regulatory region. A, schematic of the rat SNCA gene; exons are depicted as boxes and introns as lines . The specific location in the rat SNCA gene of the amplified area containing the two ZSCAN21 putative binding sites (ZSCAN21)

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Amplification, Binding Assay

    ZSCAN21 developmental neuronal co-expression with SNCA in the hippocampus region of the rat brain. A, hippocampus was isolated from different developmental stages of the rat brain as follows: E17, P1, P3, P5, P7, or P8; P10, P12, or P13; P16 and adult

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: ZSCAN21 developmental neuronal co-expression with SNCA in the hippocampus region of the rat brain. A, hippocampus was isolated from different developmental stages of the rat brain as follows: E17, P1, P3, P5, P7, or P8; P10, P12, or P13; P16 and adult

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Expressing, Isolation

    Lentivirus-mediated silencing of ZSCAN21 led to decreased levels of SNCA in differentiated neurosphere cultures. A, co-expression of ZSCAN21 and SNCA in early neurons in differentiated neurospheres. Co-localization of TUJ1 with ZSCAN21, TUJ1 with SNCA,

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: Lentivirus-mediated silencing of ZSCAN21 led to decreased levels of SNCA in differentiated neurosphere cultures. A, co-expression of ZSCAN21 and SNCA in early neurons in differentiated neurospheres. Co-localization of TUJ1 with ZSCAN21, TUJ1 with SNCA,

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Expressing

    AAV-mediated silencing of ZSCAN21 did not alter SNCA levels in the early postnatal rat brain. A, efficiency of neuronal transduction of the AAVs (AAV/shZSCAN21/2 and AAV/shscrambled) in 1-month-old rat brains. Co-localization of NeuN ( red ) and GFP ( green

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: AAV-mediated silencing of ZSCAN21 did not alter SNCA levels in the early postnatal rat brain. A, efficiency of neuronal transduction of the AAVs (AAV/shZSCAN21/2 and AAV/shscrambled) in 1-month-old rat brains. Co-localization of NeuN ( red ) and GFP ( green

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Transduction

    ZSCAN21 protein levels are under tight regulation in rat cortical neuronal cultures. Cortical neuron cultures were prepared from E17 rats. The neurons were infected 4 DIV with the AV/OVERZSCAN21 or AV/GFP (control) viruses at an m.o.i. of 100. The cultures

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: ZSCAN21 protein levels are under tight regulation in rat cortical neuronal cultures. Cortical neuron cultures were prepared from E17 rats. The neurons were infected 4 DIV with the AV/OVERZSCAN21 or AV/GFP (control) viruses at an m.o.i. of 100. The cultures

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Infection

    SNCA is increased following down-regulation of ZSCAN21 in primary cortical neuronal cultures. Cortical or hippocampal cultures were prepared from embryonic day 17 (E17) rats. The neurons were infected at 4 DIV with shZSCAN21/2, shZSCAN21/1, or shscrambled

    Journal: The Journal of Biological Chemistry

    Article Title: Complex Effects of the ZSCAN21 Transcription Factor on Transcriptional Regulation of α-Synuclein in Primary Neuronal Cultures and in Vivo *

    doi: 10.1074/jbc.M115.704973

    Figure Lengend Snippet: SNCA is increased following down-regulation of ZSCAN21 in primary cortical neuronal cultures. Cortical or hippocampal cultures were prepared from embryonic day 17 (E17) rats. The neurons were infected at 4 DIV with shZSCAN21/2, shZSCAN21/1, or shscrambled

    Article Snippet: We first performed PCR from cDNA of rat cortical cultures with specific primers to isolate the coding sequence of ZSCAN21 as follows: OVER ZSCAN21 -F′, 5′-CTGTGGATGCCAGCCCTAAA-3′, and OVER ZSCAN21 -R′, 5′-CGCTTTCTTGGGTCCTGAGT-3′.

    Techniques: Infection

    MiR-638 directly targets VEGFA ( A ) Schematic representation of the 3′UTR of VEGFA mRNA containing the putative miR-638 target site. ( B ) Relative luciferase assays comparing the SK-ES-1 and RD-ES cells transfected with pGL3-mutant (VEGFA_MUT) or pGL3-wild-type (VEGFA_WT) or untreated pGL3 vector (miR-638_PC). ( C ) The mRNA levels of VEGFA were measured by Quantitative RT-PCR in EWS cells after transfected with miR-638 or scramble mimic, respectively. ( D ) Protein levels of VEGFA and GAPDH were detected by Western blot in EWS cells after transfected with miR-638/scramble. * P

    Journal: Bioscience Reports

    Article Title: MicroRNA-638 inhibits cell growth and tubule formation by suppressing VEGFA expression in human Ewing sarcoma cells

    doi: 10.1042/BSR20171017

    Figure Lengend Snippet: MiR-638 directly targets VEGFA ( A ) Schematic representation of the 3′UTR of VEGFA mRNA containing the putative miR-638 target site. ( B ) Relative luciferase assays comparing the SK-ES-1 and RD-ES cells transfected with pGL3-mutant (VEGFA_MUT) or pGL3-wild-type (VEGFA_WT) or untreated pGL3 vector (miR-638_PC). ( C ) The mRNA levels of VEGFA were measured by Quantitative RT-PCR in EWS cells after transfected with miR-638 or scramble mimic, respectively. ( D ) Protein levels of VEGFA and GAPDH were detected by Western blot in EWS cells after transfected with miR-638/scramble. * P

    Article Snippet: The PCR primer sequences were as follows: VEGFA-F: 5′-CCCAAGCTTCGCCGCCGCTCGGCGCCCG-3′, VEGFA-R: 5′-CCGGAATTCTCACCGCTCGG CTTGTCACA-3′, the correct PCR products were verified by sequencing (Genscript, Beijing, China).

    Techniques: Luciferase, Transfection, Mutagenesis, Plasmid Preparation, Quantitative RT-PCR, Western Blot

    MiR-638 directly targets VEGFA ( A ) Schematic representation of the 3′UTR of VEGFA mRNA containing the putative miR-638 target site. ( B ) Relative luciferase assays comparing the SK-ES-1 and RD-ES cells transfected with pGL3-mutant (VEGFA_MUT) or pGL3-wild-type (VEGFA_WT) or untreated pGL3 vector (miR-638_PC). ( C ) The mRNA levels of VEGFA were measured by Quantitative RT-PCR in EWS cells after transfected with miR-638 or scramble mimic, respectively. ( D ) Protein levels of VEGFA and GAPDH were detected by Western blot in EWS cells after transfected with miR-638/scramble. * P

    Journal: Bioscience Reports

    Article Title: MicroRNA-638 inhibits cell growth and tubule formation by suppressing VEGFA expression in human Ewing sarcoma cells

    doi: 10.1042/BSR20171017

    Figure Lengend Snippet: MiR-638 directly targets VEGFA ( A ) Schematic representation of the 3′UTR of VEGFA mRNA containing the putative miR-638 target site. ( B ) Relative luciferase assays comparing the SK-ES-1 and RD-ES cells transfected with pGL3-mutant (VEGFA_MUT) or pGL3-wild-type (VEGFA_WT) or untreated pGL3 vector (miR-638_PC). ( C ) The mRNA levels of VEGFA were measured by Quantitative RT-PCR in EWS cells after transfected with miR-638 or scramble mimic, respectively. ( D ) Protein levels of VEGFA and GAPDH were detected by Western blot in EWS cells after transfected with miR-638/scramble. * P

    Article Snippet: The PCR primer sequences were as follows: VEGFA-F: 5′-CCCAAGCTTCGCCGCCGCTCGGCGCCCG-3′, VEGFA-R: 5′-CCGGAATTCTCACCGCTCGG CTTGTCACA-3′, the correct PCR products were verified by sequencing (Genscript, Beijing, China).

    Techniques: Luciferase, Transfection, Mutagenesis, Plasmid Preparation, Quantitative RT-PCR, Western Blot

    The polymorphism loci rs734553 sequencing figure in 7 introns of SLC2A9 gene A:heterozygoustype (A/C); B: homozygous type (A/A)

    Journal: Iranian Journal of Public Health

    Article Title: An Intron Variant of SLC2A9 Increases the Risk for Type 2 Diabetes Mellitus Complicated with Hyperuricemia in Chinese Male Population

    doi:

    Figure Lengend Snippet: The polymorphism loci rs734553 sequencing figure in 7 introns of SLC2A9 gene A:heterozygoustype (A/C); B: homozygous type (A/A)

    Article Snippet: The primers of rs734553 were as follows: forward 5′-GGAGAATCTGGAGCAAGT-3′ and reverse 5′-AAATAGTCCCAAAGAGTG-3′ (GenScript Biotech Co., Ltd., Nanjing, China).

    Techniques: Sequencing