primer sequences  (Beckman Coulter)


Bioz Verified Symbol Beckman Coulter is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Beckman Coulter primer sequences
    Primer Sequences, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer sequences/product/Beckman Coulter
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    primer sequences - by Bioz Stars, 2020-07
    91/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: A comprehensive immune repertoire study for patients with pulmonary tuberculosis, et al. A comprehensive immune repertoire study for patients with pulmonary tuberculosis
    Article Snippet: .. The PCR products were purified using AMPure XP beads to remove primer sequences (Beckman Coulter, Inc., Brea, CA, USA). .. A second round of PCR was performed to add a sequencing index to each sample.

    Article Title: Profiling the TRB and IGH repertoire of patients with H5N6 Avian Influenza Virus Infection by high-throughput sequencing
    Article Snippet: .. The PCR products were purified by AMPure XP beads to remove the primer sequences (Beckman Coulter, Germany). .. A second round of PCR was performed to add a sequencing index to each sample.

    Article Title: Characterization of the T-cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer
    Article Snippet: .. The PCR products were purified by AMPure XP beads to remove primer sequences (Beckman Coulter, Inc., Brea, CA, USA). .. A second round of PCR was performed to add a sequencing index to each sample.

    Article Title: Estimating and mitigating amplification bias in qualitative and quantitative arthropod metabarcoding
    Article Snippet: .. After each round of PCR, the remaining primer sequences were cleaned from the product with 1X AMpure XP Beads (Beckman Coulter, Indianapolis, USA). .. The final libraries were quantified with a Qubit Fluorometer, then all samples pooled in equimolar amounts.

    Purification:

    Article Title: A comprehensive immune repertoire study for patients with pulmonary tuberculosis, et al. A comprehensive immune repertoire study for patients with pulmonary tuberculosis
    Article Snippet: .. The PCR products were purified using AMPure XP beads to remove primer sequences (Beckman Coulter, Inc., Brea, CA, USA). .. A second round of PCR was performed to add a sequencing index to each sample.

    Article Title: Epigenomic analysis of primary human T cells reveals enhancers associated with TH2 memory cell differentiation and asthma susceptibility
    Article Snippet: .. 1 µg of this amplified cDNA was treated with restriction enzyme (SEQR, Sigma), to remove the primer sequences and then purified using AmpureXP beads (Beckman Coulter). .. Efficiency of removal of SEQR primer sequences was assessed by PCR.

    Article Title: Profiling the TRB and IGH repertoire of patients with H5N6 Avian Influenza Virus Infection by high-throughput sequencing
    Article Snippet: .. The PCR products were purified by AMPure XP beads to remove the primer sequences (Beckman Coulter, Germany). .. A second round of PCR was performed to add a sequencing index to each sample.

    Article Title: Innate-like functions of natural killer T cell subsets result from highly divergent gene programs
    Article Snippet: .. 1 µg of this amplified cDNA was treated with the restriction enzyme (SEQR, Sigma-Aldrich) to remove the primer sequences and then purified using AMPure XP beads (Beckman Coulter). .. The efficiency of removal of SEQR primer sequences was assessed by quantitative PCR.

    Article Title: Molecular Signature of Prospero Homeobox 1 (PROX1) in Follicular Thyroid Carcinoma Cells
    Article Snippet: .. The amplicons were then partially digested at primer sequences followed by the adapters’ ligation to amplicons and purification on AMPure XP beads (Beckman Coulter Inc., Brea, CA, USA). ..

    Article Title: Characterization of the T-cell receptor repertoire by deep T cell receptor sequencing in tissues from patients with prostate cancer
    Article Snippet: .. The PCR products were purified by AMPure XP beads to remove primer sequences (Beckman Coulter, Inc., Brea, CA, USA). .. A second round of PCR was performed to add a sequencing index to each sample.

    Amplification:

    Article Title: Epigenomic analysis of primary human T cells reveals enhancers associated with TH2 memory cell differentiation and asthma susceptibility
    Article Snippet: .. 1 µg of this amplified cDNA was treated with restriction enzyme (SEQR, Sigma), to remove the primer sequences and then purified using AmpureXP beads (Beckman Coulter). .. Efficiency of removal of SEQR primer sequences was assessed by PCR.

    Article Title: Innate-like functions of natural killer T cell subsets result from highly divergent gene programs
    Article Snippet: .. 1 µg of this amplified cDNA was treated with the restriction enzyme (SEQR, Sigma-Aldrich) to remove the primer sequences and then purified using AMPure XP beads (Beckman Coulter). .. The efficiency of removal of SEQR primer sequences was assessed by quantitative PCR.

    Ligation:

    Article Title: Molecular Signature of Prospero Homeobox 1 (PROX1) in Follicular Thyroid Carcinoma Cells
    Article Snippet: .. The amplicons were then partially digested at primer sequences followed by the adapters’ ligation to amplicons and purification on AMPure XP beads (Beckman Coulter Inc., Brea, CA, USA). ..

    Software:

    Article Title: Further Examination of the Candidate Genes in Chromosome 12p13 Locus for Late-Onset Alzheimer Disease
    Article Snippet: .. Primer sequences were designed using software provided at (Beckman Coulter Inc., Fullerton, CA) and are available upon request. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Beckman Coulter anammox specific primers
    Denaturing gradient gel electrophoresis (DGGE) profiles of <t>anammox</t> bacterial 16S rRNA genes. The two main primer sets were used to generate the anammox-fingerprints (A and D) by direct PCR amplification. The additional four patterns (B, C, E and F) were produced by the nested PCR assay, using other two primer sets and followed by the two main sets. Triangles indicate a total of 55 bands, associated with anammox bacteria. Only three representative bands were shown in the phylogenetic tree. Bands 2-1, 2-2 and 2-3 were indicated by the blue, green and red triangles, respectively. Each coloured triangle indicates exactly the same phylogeny shown in Fig. 2 .
    Anammox Specific Primers, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anammox specific primers/product/Beckman Coulter
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anammox specific primers - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    85
    Beckman Coulter fli1 primer
    Genomic fusion site sequencing. (A) Genomic organization of the EWS and <t>FLI1</t> genes and corresponding breakpoint cluster regions (BCR). Nested primer sets for der22 are shown as double headed arrows. (B) Representative breakpoint sequencing workflow. Left: Gel electrophoresis of MLR-PCR products from two tumor samples in lane 1 and 2 (lane 3 negative control DNA; lane 4 ddH 2 O; lane 5 positive control DNA; M = DNA ladder). Center: Gel electrophoresis of single long-range PCR products from 1 st round MLR-PCR product of sample 1 (lane 1–11; lane 12 positive control) to identify FLI1 and EWS primers next to the fusion sites and to reduce amplification product size for direct sequencing. Right: Sequencing of the shortest amplification product and alignment to EWS and FLI1 reference sequences.
    Fli1 Primer, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fli1 primer/product/Beckman Coulter
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fli1 primer - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    80
    Beckman Coulter human bcl 2 genes
    Survival analysis of CrmA-transfected rat Schwann cells in vitro after survival factor withdrawal or treatment with exogenous NGF. A , CrmA-transfected cells show delayed but retained susceptibility to survival factor deprivation but are protected against NGF killing. Shown are the mean viabilities ±SEM of three separate CrmA-transfected Schwann cell lines (CrmA#7, #14, and #15) in comparison with the pEF#1 control cell line, as assessed in one of two similar survival experiments. Cell lines were cultured either in DMEM only or in DMEM containing 10 ng/ml NGF. Control pEF cells died rapidly in both conditions (only DMEM is shown). CrmA-transfected cells showed some abrogated death at 24 hr ( p = 0.025), but by 72 hr both the CrmA and control cells showed a similar death profile ( p values 0.1 at 48 hr and 0.15 at 72 hr; NS). B , CrmA-transfected cells are resistant to NGF killing in the presence of IGF-1, whereas <t>Bcl-2-transfected</t> Schwann cells remain susceptible to NGF under these conditions. Shown are means ± SEM of three separate experiments using the cell lines Bcl-2#1 and CrmA#7. The cells were cultured in either DMEM containing 100 ng/ml IGF-1 or DMEM containing 100 ng/ml IGF-1 and 10 ng/ml NGF. The difference in survival between the two culture conditions was significant for the Bcl-2#1 cell line ( p values from 0.025 to 0.005), but not for the CrmA#7 cell line ( p > 0.05).
    Human Bcl 2 Genes, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bcl 2 genes/product/Beckman Coulter
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human bcl 2 genes - by Bioz Stars, 2020-07
    80/100 stars
      Buy from Supplier

    85
    Beckman Coulter cyslt1r primers
    Images of agarose gels (1.5%) stained with EtBr. Expression of cysteine leukotriene ( <t>CysLT1R</t> ) and von Willebrand factor ( vWF ) genes in A ) normal chinchilla middle ear mucosa (MEM) and B ) Haemophilus influenzae –infected MEM. –T — no template RNA/DNA added to polymerase chain reaction (PCR); –RT — PCR performed without prior reverse transcriptase step in presence of template RNA; +T — template RNA plus reverse transcription (RT) before PCR. +T reaction using CysLT1R-specific primers from both normal and infected MEM yield predicted 211 bp PCR product, and +T reactions for vWF gene–specific primers yielded expected 266 bp PCR product.
    Cyslt1r Primers, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyslt1r primers/product/Beckman Coulter
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cyslt1r primers - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    Denaturing gradient gel electrophoresis (DGGE) profiles of anammox bacterial 16S rRNA genes. The two main primer sets were used to generate the anammox-fingerprints (A and D) by direct PCR amplification. The additional four patterns (B, C, E and F) were produced by the nested PCR assay, using other two primer sets and followed by the two main sets. Triangles indicate a total of 55 bands, associated with anammox bacteria. Only three representative bands were shown in the phylogenetic tree. Bands 2-1, 2-2 and 2-3 were indicated by the blue, green and red triangles, respectively. Each coloured triangle indicates exactly the same phylogeny shown in Fig. 2 .

    Journal: PLoS ONE

    Article Title: Evaluating Primers for Profiling Anaerobic Ammonia Oxidizing Bacteria within Freshwater Environments

    doi: 10.1371/journal.pone.0057242

    Figure Lengend Snippet: Denaturing gradient gel electrophoresis (DGGE) profiles of anammox bacterial 16S rRNA genes. The two main primer sets were used to generate the anammox-fingerprints (A and D) by direct PCR amplification. The additional four patterns (B, C, E and F) were produced by the nested PCR assay, using other two primer sets and followed by the two main sets. Triangles indicate a total of 55 bands, associated with anammox bacteria. Only three representative bands were shown in the phylogenetic tree. Bands 2-1, 2-2 and 2-3 were indicated by the blue, green and red triangles, respectively. Each coloured triangle indicates exactly the same phylogeny shown in Fig. 2 .

    Article Snippet: Representative bands were excised and sequenced by the corresponding anammox-specific primers at Beckman Coulter Genomics using an ABI 3730XL sequencer.

    Techniques: Denaturing Gradient Gel Electrophoresis, Polymerase Chain Reaction, Amplification, Produced, Nested PCR

    Abundance of anammox bacterial 16S rRNA genes quantified by two anammox specific primer sets, and general bacterial qPCR data for comparison. The qPCR efficiency ( E ) and coefficient of determination ( R 2 ) of each primer set are shown in parentheses.

    Journal: PLoS ONE

    Article Title: Evaluating Primers for Profiling Anaerobic Ammonia Oxidizing Bacteria within Freshwater Environments

    doi: 10.1371/journal.pone.0057242

    Figure Lengend Snippet: Abundance of anammox bacterial 16S rRNA genes quantified by two anammox specific primer sets, and general bacterial qPCR data for comparison. The qPCR efficiency ( E ) and coefficient of determination ( R 2 ) of each primer set are shown in parentheses.

    Article Snippet: Representative bands were excised and sequenced by the corresponding anammox-specific primers at Beckman Coulter Genomics using an ABI 3730XL sequencer.

    Techniques: Real-time Polymerase Chain Reaction

    Denaturing gradient gel electrophoresis (DGGE) profiles of bacterial and anammox bacterial 16S rRNA genes in comparison. Together with six environmental samples, positive control template ( Ca. Jettenia, Ca. Brocadia and Ca. Scalindua) and a negative control ( E. coli ) were included. Triangles indicate sequenced bands. Band 1-1, indicated by a yellow triangle, was associated with Unknown anammox cluster 1 [17] and band 1-2, indicated by a purple triangle, was affiliated with Unknown anammox cluster 2 [17] . Bands indicated by black triangles were not affiliated with anammox bacteria. A star indicates a band with low quality sequence, which was excluded from subsequent phylogenetic analysis.

    Journal: PLoS ONE

    Article Title: Evaluating Primers for Profiling Anaerobic Ammonia Oxidizing Bacteria within Freshwater Environments

    doi: 10.1371/journal.pone.0057242

    Figure Lengend Snippet: Denaturing gradient gel electrophoresis (DGGE) profiles of bacterial and anammox bacterial 16S rRNA genes in comparison. Together with six environmental samples, positive control template ( Ca. Jettenia, Ca. Brocadia and Ca. Scalindua) and a negative control ( E. coli ) were included. Triangles indicate sequenced bands. Band 1-1, indicated by a yellow triangle, was associated with Unknown anammox cluster 1 [17] and band 1-2, indicated by a purple triangle, was affiliated with Unknown anammox cluster 2 [17] . Bands indicated by black triangles were not affiliated with anammox bacteria. A star indicates a band with low quality sequence, which was excluded from subsequent phylogenetic analysis.

    Article Snippet: Representative bands were excised and sequenced by the corresponding anammox-specific primers at Beckman Coulter Genomics using an ABI 3730XL sequencer.

    Techniques: Denaturing Gradient Gel Electrophoresis, Environmental Sampling, Positive Control, Negative Control, Sequencing

    Phylogenetic tree of anammox bacterial 16S rRNA genes retrieved from both DGGE and clone library methods (shown in bold). All DGGE band sequences are indicated by “DGGE” with representative band ID, related to Fig. 1 and 3 . The number of individual DGGE bands is indicated by the triangles with corresponding colour. Clone sequences with 97% identity from each library were grouped together; the representative clones from each operational taxonomic unit (OTU) were included in the analysis. The number of sequences belonging to each OTU is indicated in parentheses. The maximum likelihood tree was constructed with the GTR model. Branch support values (aLRT) greater than 50% are indicated at the nodes.

    Journal: PLoS ONE

    Article Title: Evaluating Primers for Profiling Anaerobic Ammonia Oxidizing Bacteria within Freshwater Environments

    doi: 10.1371/journal.pone.0057242

    Figure Lengend Snippet: Phylogenetic tree of anammox bacterial 16S rRNA genes retrieved from both DGGE and clone library methods (shown in bold). All DGGE band sequences are indicated by “DGGE” with representative band ID, related to Fig. 1 and 3 . The number of individual DGGE bands is indicated by the triangles with corresponding colour. Clone sequences with 97% identity from each library were grouped together; the representative clones from each operational taxonomic unit (OTU) were included in the analysis. The number of sequences belonging to each OTU is indicated in parentheses. The maximum likelihood tree was constructed with the GTR model. Branch support values (aLRT) greater than 50% are indicated at the nodes.

    Article Snippet: Representative bands were excised and sequenced by the corresponding anammox-specific primers at Beckman Coulter Genomics using an ABI 3730XL sequencer.

    Techniques: Denaturing Gradient Gel Electrophoresis, Clone Assay, Construct

    Genomic fusion site sequencing. (A) Genomic organization of the EWS and FLI1 genes and corresponding breakpoint cluster regions (BCR). Nested primer sets for der22 are shown as double headed arrows. (B) Representative breakpoint sequencing workflow. Left: Gel electrophoresis of MLR-PCR products from two tumor samples in lane 1 and 2 (lane 3 negative control DNA; lane 4 ddH 2 O; lane 5 positive control DNA; M = DNA ladder). Center: Gel electrophoresis of single long-range PCR products from 1 st round MLR-PCR product of sample 1 (lane 1–11; lane 12 positive control) to identify FLI1 and EWS primers next to the fusion sites and to reduce amplification product size for direct sequencing. Right: Sequencing of the shortest amplification product and alignment to EWS and FLI1 reference sequences.

    Journal: PLoS ONE

    Article Title: Genomic EWS-FLI1 Fusion Sequences in Ewing Sarcoma Resemble Breakpoint Characteristics of Immature Lymphoid Malignancies

    doi: 10.1371/journal.pone.0056408

    Figure Lengend Snippet: Genomic fusion site sequencing. (A) Genomic organization of the EWS and FLI1 genes and corresponding breakpoint cluster regions (BCR). Nested primer sets for der22 are shown as double headed arrows. (B) Representative breakpoint sequencing workflow. Left: Gel electrophoresis of MLR-PCR products from two tumor samples in lane 1 and 2 (lane 3 negative control DNA; lane 4 ddH 2 O; lane 5 positive control DNA; M = DNA ladder). Center: Gel electrophoresis of single long-range PCR products from 1 st round MLR-PCR product of sample 1 (lane 1–11; lane 12 positive control) to identify FLI1 and EWS primers next to the fusion sites and to reduce amplification product size for direct sequencing. Right: Sequencing of the shortest amplification product and alignment to EWS and FLI1 reference sequences.

    Article Snippet: Subsequently, the appropriate FLI1 primer was used in a series of single LR-PCRs in combination with additional internal sense EWS primers to further reduce the size of the specific amplification product for direct sequencing on a Beckman Coulter CEQ 8800 Genetic Analysis System ( middle, right side).

    Techniques: Sequencing, Nucleic Acid Electrophoresis, Polymerase Chain Reaction, Negative Control, Positive Control, Amplification

    Breakpoint distribution in the BCR of EWS and FLl1 . (A) Vertical bars above or below the breakpoint regions indicate individual breakpoint positions of Ewing sarcoma patients. Black boxes represent exons, and gray boxes correspond to repeat elements. (B) Results of Kernel density analysis (dashed line = breakpoint density; gray line = lower limit of 95% confidence band determined by bootstrapping procedure; black line = 95% confidence interval of a density function resulting from simulations at randomly distributed pseudo-breakpoints). X-axes indicate the BCR nucleotide positions within the respective reference gene. (C) Scatterblot of gender-specific EWS-FLI1 breakpoints. Circles represent female, and squares represent male subjects. (D) Number of microhomologies and filler nucleotides at EWS-FLI1 (der22; black bar) and FLI1-EWS (der11; gray bar) fusion sites. Each bar on the x-axis represents one individual.

    Journal: PLoS ONE

    Article Title: Genomic EWS-FLI1 Fusion Sequences in Ewing Sarcoma Resemble Breakpoint Characteristics of Immature Lymphoid Malignancies

    doi: 10.1371/journal.pone.0056408

    Figure Lengend Snippet: Breakpoint distribution in the BCR of EWS and FLl1 . (A) Vertical bars above or below the breakpoint regions indicate individual breakpoint positions of Ewing sarcoma patients. Black boxes represent exons, and gray boxes correspond to repeat elements. (B) Results of Kernel density analysis (dashed line = breakpoint density; gray line = lower limit of 95% confidence band determined by bootstrapping procedure; black line = 95% confidence interval of a density function resulting from simulations at randomly distributed pseudo-breakpoints). X-axes indicate the BCR nucleotide positions within the respective reference gene. (C) Scatterblot of gender-specific EWS-FLI1 breakpoints. Circles represent female, and squares represent male subjects. (D) Number of microhomologies and filler nucleotides at EWS-FLI1 (der22; black bar) and FLI1-EWS (der11; gray bar) fusion sites. Each bar on the x-axis represents one individual.

    Article Snippet: Subsequently, the appropriate FLI1 primer was used in a series of single LR-PCRs in combination with additional internal sense EWS primers to further reduce the size of the specific amplification product for direct sequencing on a Beckman Coulter CEQ 8800 Genetic Analysis System ( middle, right side).

    Techniques:

    Survival analysis of CrmA-transfected rat Schwann cells in vitro after survival factor withdrawal or treatment with exogenous NGF. A , CrmA-transfected cells show delayed but retained susceptibility to survival factor deprivation but are protected against NGF killing. Shown are the mean viabilities ±SEM of three separate CrmA-transfected Schwann cell lines (CrmA#7, #14, and #15) in comparison with the pEF#1 control cell line, as assessed in one of two similar survival experiments. Cell lines were cultured either in DMEM only or in DMEM containing 10 ng/ml NGF. Control pEF cells died rapidly in both conditions (only DMEM is shown). CrmA-transfected cells showed some abrogated death at 24 hr ( p = 0.025), but by 72 hr both the CrmA and control cells showed a similar death profile ( p values 0.1 at 48 hr and 0.15 at 72 hr; NS). B , CrmA-transfected cells are resistant to NGF killing in the presence of IGF-1, whereas Bcl-2-transfected Schwann cells remain susceptible to NGF under these conditions. Shown are means ± SEM of three separate experiments using the cell lines Bcl-2#1 and CrmA#7. The cells were cultured in either DMEM containing 100 ng/ml IGF-1 or DMEM containing 100 ng/ml IGF-1 and 10 ng/ml NGF. The difference in survival between the two culture conditions was significant for the Bcl-2#1 cell line ( p values from 0.025 to 0.005), but not for the CrmA#7 cell line ( p > 0.05).

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: Survival analysis of CrmA-transfected rat Schwann cells in vitro after survival factor withdrawal or treatment with exogenous NGF. A , CrmA-transfected cells show delayed but retained susceptibility to survival factor deprivation but are protected against NGF killing. Shown are the mean viabilities ±SEM of three separate CrmA-transfected Schwann cell lines (CrmA#7, #14, and #15) in comparison with the pEF#1 control cell line, as assessed in one of two similar survival experiments. Cell lines were cultured either in DMEM only or in DMEM containing 10 ng/ml NGF. Control pEF cells died rapidly in both conditions (only DMEM is shown). CrmA-transfected cells showed some abrogated death at 24 hr ( p = 0.025), but by 72 hr both the CrmA and control cells showed a similar death profile ( p values 0.1 at 48 hr and 0.15 at 72 hr; NS). B , CrmA-transfected cells are resistant to NGF killing in the presence of IGF-1, whereas Bcl-2-transfected Schwann cells remain susceptible to NGF under these conditions. Shown are means ± SEM of three separate experiments using the cell lines Bcl-2#1 and CrmA#7. The cells were cultured in either DMEM containing 100 ng/ml IGF-1 or DMEM containing 100 ng/ml IGF-1 and 10 ng/ml NGF. The difference in survival between the two culture conditions was significant for the Bcl-2#1 cell line ( p values from 0.025 to 0.005), but not for the CrmA#7 cell line ( p > 0.05).

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Transfection, In Vitro, Cell Culture

    Nerve growth factor does not induce cleavage of Bcl-2. Western blotting of Bcl-2-transfected and control Schwann cells was performed to determine whether NGF induces cleavage of Bcl-2. Lane 1 , Wild-type rat Schwann cells; lane 2 , Bcl-2-transfected Schwann cells in serum, neuregulin-β, and forskolin; lanes 3 and 4 , survival factor-deprived condition for 24 hr; lanes 5 and 6 , survival factor-deprived condition for 72 hr. No cleavage product was detected when 10 ng/ml NGF was present in the survival factor-deprived conditions ( lanes 4 and 6 ).

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: Nerve growth factor does not induce cleavage of Bcl-2. Western blotting of Bcl-2-transfected and control Schwann cells was performed to determine whether NGF induces cleavage of Bcl-2. Lane 1 , Wild-type rat Schwann cells; lane 2 , Bcl-2-transfected Schwann cells in serum, neuregulin-β, and forskolin; lanes 3 and 4 , survival factor-deprived condition for 24 hr; lanes 5 and 6 , survival factor-deprived condition for 72 hr. No cleavage product was detected when 10 ng/ml NGF was present in the survival factor-deprived conditions ( lanes 4 and 6 ).

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Western Blot, Transfection

    The transfected Schwann cells express the astroglial protein S-100. Immunoperoxidase staining of Bcl-2#1 cells ( B ) and CrmA#14-cells ( C ) with rabbit anti-cow-S-100 protein is shown. As negative controls, the same cell lines were stained with secondary antibody only, as shown for the Bcl-2#1 cell line in A . Scale bar (shown in A ): A , B , 50 μm; C , 100 μm.

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: The transfected Schwann cells express the astroglial protein S-100. Immunoperoxidase staining of Bcl-2#1 cells ( B ) and CrmA#14-cells ( C ) with rabbit anti-cow-S-100 protein is shown. As negative controls, the same cell lines were stained with secondary antibody only, as shown for the Bcl-2#1 cell line in A . Scale bar (shown in A ): A , B , 50 μm; C , 100 μm.

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Transfection, Immunoperoxidase Staining, Staining

    Transcripts of the high-affinity NGF receptor TrkA are not detectable in the Bcl-2-transfected Schwann cells. Northern blots were prepared using mRNA (∼0.5 μg/lane) isolated from Bcl-2#1 Schwann cells cultured in DMEM containing 10 ng/ml NGF for 4 hr ( lane 1 ) or on survival factor withdrawal for 4 hr ( lane 2 ) or in DMEM containing serum, neuregulin-β, and forskolin ( lane 3 ). Approximately 10 μg of total RNA isolated from PC12 neuronal cells was loaded as a positive control in lane 5 . The arrow indicates the TrkA transcript between the 28S and 18S ribosomal RNA bands that was detected in RNA isolated from the PC12 cells.

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: Transcripts of the high-affinity NGF receptor TrkA are not detectable in the Bcl-2-transfected Schwann cells. Northern blots were prepared using mRNA (∼0.5 μg/lane) isolated from Bcl-2#1 Schwann cells cultured in DMEM containing 10 ng/ml NGF for 4 hr ( lane 1 ) or on survival factor withdrawal for 4 hr ( lane 2 ) or in DMEM containing serum, neuregulin-β, and forskolin ( lane 3 ). Approximately 10 μg of total RNA isolated from PC12 neuronal cells was loaded as a positive control in lane 5 . The arrow indicates the TrkA transcript between the 28S and 18S ribosomal RNA bands that was detected in RNA isolated from the PC12 cells.

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Transfection, Northern Blot, Isolation, Cell Culture, Positive Control

    Bcl-2-transfected, CrmA-transfected, and control rat Schwann cells express the low-affinity NGF receptor p75 on the cell surface. Approximately 10 6 pEF, Bcl-2#1, Bcl-2#3, Bcl-2#4, CrmA#7, or CrmA#14 cells were stained with mAb MC192 against the rat p75 receptor or with mouse IgG1 control mAb, followed by FITC-conjugated sheep anti-mouse IgG. Fluorescence was measured using a flow cytometer. The background fluorescence obtained with the isotype-matched control mAb ( dotted lines ) was subtracted from the total pool to enable calculation of the percentages of cells from each cell line that expressed p75 (labeled as the M1 population).

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: Bcl-2-transfected, CrmA-transfected, and control rat Schwann cells express the low-affinity NGF receptor p75 on the cell surface. Approximately 10 6 pEF, Bcl-2#1, Bcl-2#3, Bcl-2#4, CrmA#7, or CrmA#14 cells were stained with mAb MC192 against the rat p75 receptor or with mouse IgG1 control mAb, followed by FITC-conjugated sheep anti-mouse IgG. Fluorescence was measured using a flow cytometer. The background fluorescence obtained with the isotype-matched control mAb ( dotted lines ) was subtracted from the total pool to enable calculation of the percentages of cells from each cell line that expressed p75 (labeled as the M1 population).

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Transfection, Staining, Fluorescence, Flow Cytometry, Cytometry, Labeling

    Intracellular expression of the human Bcl-2 protein and the poxvirus caspase inhibitor CrmA in the transfected rat Schwann cell lines Bcl-2#1, -#3, -#4, and CrmA#7, #14, and #15. Immunofluorescence staining was performed with the mouse mAb Bcl-2–100 against the human Bcl-2 (for the Bcl-2 cell lines) or with mouse anti-Flag mAb (for the CrmA cell lines), and binding of primary antibody was detected with FITC-conjugated sheep anti-mouse IgG. As negative controls, rat Schwann cells transfected with the empty expression plasmid pEF were similarly stained (indicated in the histograms with a dashed line ).

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: Intracellular expression of the human Bcl-2 protein and the poxvirus caspase inhibitor CrmA in the transfected rat Schwann cell lines Bcl-2#1, -#3, -#4, and CrmA#7, #14, and #15. Immunofluorescence staining was performed with the mouse mAb Bcl-2–100 against the human Bcl-2 (for the Bcl-2 cell lines) or with mouse anti-Flag mAb (for the CrmA cell lines), and binding of primary antibody was detected with FITC-conjugated sheep anti-mouse IgG. As negative controls, rat Schwann cells transfected with the empty expression plasmid pEF were similarly stained (indicated in the histograms with a dashed line ).

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Expressing, Transfection, Immunofluorescence, Staining, Binding Assay, Plasmid Preparation

    Regulation of Bcl-2 expression in wild-type rat Schwann cells as studied by RT-PCR. Bcl-2 expression was detected in cells cultured in IGF-1 for 4 hr ( lane 4 ), IGF-1 for 16 hr ( lane 5 ), NGF for 4 hr ( lane 6 ), and after withdrawal of FCS, neuregulin-β, and forskolin for 1 hr ( lane 7 ) or 2 hr ( lane 8 ). Bcl-2 was downregulated to undetectable levels after 4, 8, and 24 hr of survival factor deprivation ( lanes 9–11 ). Rat β-actin was amplified as control to verify the fidelity of the cDNA. A φX174– Hae III digest in lane 0 served as a molecular weight marker. Negative controls included no DNA in lane 1 and tail DNA from a Bcl-2 knockout mouse (Bcl-2 −/− ) in lane 2 . Tail DNA from a Bcl-2 wild-type littermate of the Bcl-2 −/− mouse served as a positive control ( lane 3 , Bcl-2 +/+ ).

    Journal: The Journal of Neuroscience

    Article Title: Nerve Growth Factor Signaling through p75 Induces Apoptosis in Schwann Cells via a Bcl-2-Independent Pathway

    doi: 10.1523/JNEUROSCI.19-12-04828.1999

    Figure Lengend Snippet: Regulation of Bcl-2 expression in wild-type rat Schwann cells as studied by RT-PCR. Bcl-2 expression was detected in cells cultured in IGF-1 for 4 hr ( lane 4 ), IGF-1 for 16 hr ( lane 5 ), NGF for 4 hr ( lane 6 ), and after withdrawal of FCS, neuregulin-β, and forskolin for 1 hr ( lane 7 ) or 2 hr ( lane 8 ). Bcl-2 was downregulated to undetectable levels after 4, 8, and 24 hr of survival factor deprivation ( lanes 9–11 ). Rat β-actin was amplified as control to verify the fidelity of the cDNA. A φX174– Hae III digest in lane 0 served as a molecular weight marker. Negative controls included no DNA in lane 1 and tail DNA from a Bcl-2 knockout mouse (Bcl-2 −/− ) in lane 2 . Tail DNA from a Bcl-2 wild-type littermate of the Bcl-2 −/− mouse served as a positive control ( lane 3 , Bcl-2 +/+ ).

    Article Snippet: These primers were designed to maximize amplification of sequences similar in the rat, mouse, and human Bcl-2 genes, with minimal homology to other Bcl family members, and were synthesized by Beckman Oligonucleotide Synthesis Service (Gladesville, NSW, Australia).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Amplification, Molecular Weight, Marker, Knock-Out, Positive Control

    Images of agarose gels (1.5%) stained with EtBr. Expression of cysteine leukotriene ( CysLT1R ) and von Willebrand factor ( vWF ) genes in A ) normal chinchilla middle ear mucosa (MEM) and B ) Haemophilus influenzae –infected MEM. –T — no template RNA/DNA added to polymerase chain reaction (PCR); –RT — PCR performed without prior reverse transcriptase step in presence of template RNA; +T — template RNA plus reverse transcription (RT) before PCR. +T reaction using CysLT1R-specific primers from both normal and infected MEM yield predicted 211 bp PCR product, and +T reactions for vWF gene–specific primers yielded expected 266 bp PCR product.

    Journal: The Annals of otology, rhinology, and laryngology

    Article Title: Partial Characterization of Normal and Haemophilus influenzae-Infected Mucosal Complementary DNA Libraries in Chinchilla Middle Ear Mucosa

    doi:

    Figure Lengend Snippet: Images of agarose gels (1.5%) stained with EtBr. Expression of cysteine leukotriene ( CysLT1R ) and von Willebrand factor ( vWF ) genes in A ) normal chinchilla middle ear mucosa (MEM) and B ) Haemophilus influenzae –infected MEM. –T — no template RNA/DNA added to polymerase chain reaction (PCR); –RT — PCR performed without prior reverse transcriptase step in presence of template RNA; +T — template RNA plus reverse transcription (RT) before PCR. +T reaction using CysLT1R-specific primers from both normal and infected MEM yield predicted 211 bp PCR product, and +T reactions for vWF gene–specific primers yielded expected 266 bp PCR product.

    Article Snippet: The nucleotide sequence of cDNA generated from the CysLT1R primers was determined on a Beckman CEQ8000 automated capillary DNA sequencer.

    Techniques: Staining, Expressing, Infection, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    Phylogenetic trees demonstrate that chinchilla CysLT1R , CysLT2R , and LTA4 hydrolase genes have generally greater similarity to humans than other rodents.

    Journal: The Annals of otology, rhinology, and laryngology

    Article Title: Partial Characterization of Normal and Haemophilus influenzae-Infected Mucosal Complementary DNA Libraries in Chinchilla Middle Ear Mucosa

    doi:

    Figure Lengend Snippet: Phylogenetic trees demonstrate that chinchilla CysLT1R , CysLT2R , and LTA4 hydrolase genes have generally greater similarity to humans than other rodents.

    Article Snippet: The nucleotide sequence of cDNA generated from the CysLT1R primers was determined on a Beckman CEQ8000 automated capillary DNA sequencer.

    Techniques: