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Illumina Inc primer oligonucleotides
Primer Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer oligonucleotides/product/Illumina Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
primer oligonucleotides - by Bioz Stars, 2020-04
86/100 stars

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Sequencing:

Article Title: Exome Sequencing of Germline DNA from Non-BRCA1/2 Familial Breast Cancer Cases Selected on the Basis of aCGH Tumor Profiling
Article Snippet: .. Primer oligonucleotides for paired-end sequencing (Illumina) were ligated to both ends of the fragment. ..

Flow Cytometry:

Article Title: Exome Sequencing of Germline DNA from Non-BRCA1/2 Familial Breast Cancer Cases Selected on the Basis of aCGH Tumor Profiling
Article Snippet: Primer oligonucleotides for paired-end sequencing (Illumina) were ligated to both ends of the fragment. .. Paired-end flow cells were then prepared on a cluster station according to the manufactures protocol (Illumina), using one lane per sample.

Amplification:

Article Title: Exome Sequencing of Germline DNA from Non-BRCA1/2 Familial Breast Cancer Cases Selected on the Basis of aCGH Tumor Profiling
Article Snippet: Primer oligonucleotides for paired-end sequencing (Illumina) were ligated to both ends of the fragment. .. After multiple washing steps, the captured DNA was amplified in order to get sufficient DNA for the sequencing experiment.

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  • 98
    Illumina Inc illumina miniseq sequencer
    Effect of phiX spike-in on observed microbial communities with amplicons generated using ShortEMP primers. Genus-level annotations of sequence data generated on an <t>Illumina</t> <t>MiniSeq</t> instrument were visualized using mMDS ordination employing a distance matrix based on Bray–Curtis similarity. Rarefaction was performed to a depth of 30,000 sequences per sample. For each ShortEMP PCR condition, six technical replicates were analyzed using either 20% or 1.75% phiX spike-in. Small differences, consistent with run-to-run variation, were observed between the two sequencing runs (ANOSIM: 40 °C R = 0.146, p = 0.052; 45 °C R = 0.394, p = 0.011; 50 °C R = 0.178, p = 0.100). Ellipses represent a 95% confidence interval around the centroid.
    Illumina Miniseq Sequencer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 98/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina miniseq sequencer/product/Illumina Inc
    Average 98 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    illumina miniseq sequencer - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    99
    Illumina Inc illumina sequencing primer
    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the <t>Illumina</t> sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.
    Illumina Sequencing Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina sequencing primer/product/Illumina Inc
    Average 99 stars, based on 89 article reviews
    Price from $9.99 to $1999.99
    illumina sequencing primer - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    87
    Illumina Inc qrt pcr primers
    Validation of RNA-Seq data by <t>qRT–PCR.</t> Nine PTI-, ETI-, and defence-related genes were selected for validation.
    Qrt Pcr Primers, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qrt pcr primers/product/Illumina Inc
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qrt pcr primers - by Bioz Stars, 2020-04
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    99
    Illumina Inc transcriptomes
    Gene ontology (GO) classification for the P . delavayi <t>transcriptome.</t> The transcripts (33,249) were categorized into 55 function groups. The right y-axis indicates the number of genes in a category, whereas the left y-axis indicates the percentage of a specific category of genes in the corresponding main category.
    Transcriptomes, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 352 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptomes/product/Illumina Inc
    Average 99 stars, based on 352 article reviews
    Price from $9.99 to $1999.99
    transcriptomes - by Bioz Stars, 2020-04
    99/100 stars
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    Image Search Results


    Effect of phiX spike-in on observed microbial communities with amplicons generated using ShortEMP primers. Genus-level annotations of sequence data generated on an Illumina MiniSeq instrument were visualized using mMDS ordination employing a distance matrix based on Bray–Curtis similarity. Rarefaction was performed to a depth of 30,000 sequences per sample. For each ShortEMP PCR condition, six technical replicates were analyzed using either 20% or 1.75% phiX spike-in. Small differences, consistent with run-to-run variation, were observed between the two sequencing runs (ANOSIM: 40 °C R = 0.146, p = 0.052; 45 °C R = 0.394, p = 0.011; 50 °C R = 0.178, p = 0.100). Ellipses represent a 95% confidence interval around the centroid.

    Journal: PeerJ

    Article Title: PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

    doi: 10.7717/peerj.6570

    Figure Lengend Snippet: Effect of phiX spike-in on observed microbial communities with amplicons generated using ShortEMP primers. Genus-level annotations of sequence data generated on an Illumina MiniSeq instrument were visualized using mMDS ordination employing a distance matrix based on Bray–Curtis similarity. Rarefaction was performed to a depth of 30,000 sequences per sample. For each ShortEMP PCR condition, six technical replicates were analyzed using either 20% or 1.75% phiX spike-in. Small differences, consistent with run-to-run variation, were observed between the two sequencing runs (ANOSIM: 40 °C R = 0.146, p = 0.052; 45 °C R = 0.394, p = 0.011; 50 °C R = 0.178, p = 0.100). Ellipses represent a 95% confidence interval around the centroid.

    Article Snippet: When amplicons generated with the modified primers were sequenced on an Illumina MiniSeq sequencer with < 2% phiX, the data quality and community analyses were consistent with the same amplicons generated on a run with 20% phiX.

    Techniques: Generated, Sequencing, Polymerase Chain Reaction

    Effect of annealing temperature and primer set on observed microbial community in Lake Michigan sediment. A composite sample of gDNA from Lake Michigan sediment was amplified using EMP or ShortEMP primer pools at annealing temperatures of 40, 45 and 50 °C and sequenced on an Illumina MiniSeq instrument. Sequence data were rarefied to a depth of 17,500 sequences per sample. (A) Genus-level annotations of sequence data were visualized using metric multidimensional scaling (mMDS) employing a distance matrix based on Bray–Curtis similarity. Across all temperatures, the use of EMP and ShortEMP primers resulted in slightly, but significantly, different observed microbial communities (ANOSIM R = 0.473; p

    Journal: PeerJ

    Article Title: PCR effects of melting temperature adjustment of individual primers in degenerate primer pools

    doi: 10.7717/peerj.6570

    Figure Lengend Snippet: Effect of annealing temperature and primer set on observed microbial community in Lake Michigan sediment. A composite sample of gDNA from Lake Michigan sediment was amplified using EMP or ShortEMP primer pools at annealing temperatures of 40, 45 and 50 °C and sequenced on an Illumina MiniSeq instrument. Sequence data were rarefied to a depth of 17,500 sequences per sample. (A) Genus-level annotations of sequence data were visualized using metric multidimensional scaling (mMDS) employing a distance matrix based on Bray–Curtis similarity. Across all temperatures, the use of EMP and ShortEMP primers resulted in slightly, but significantly, different observed microbial communities (ANOSIM R = 0.473; p

    Article Snippet: When amplicons generated with the modified primers were sequenced on an Illumina MiniSeq sequencer with < 2% phiX, the data quality and community analyses were consistent with the same amplicons generated on a run with 20% phiX.

    Techniques: Amplification, Sequencing

    Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the Illumina sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.

    Journal: PLoS Genetics

    Article Title: The Serum Resistome of a Globally Disseminated Multidrug Resistant Uropathogenic Escherichia coli Clone

    doi: 10.1371/journal.pgen.1003834

    Figure Lengend Snippet: Experimental design to identify serum resistance genes in EC958. (A) Selection steps employed using fresh serum as test and inactivated serum as control. (B) Schematic illustration of the Illumina sequencing procedure including the use of a custom oligo for indexing and enrichment of insert sites.

    Article Snippet: This oligonucleotide incorporates the Illumina sequencing primer-binding site into transposon specific DNA fragments, enabling the use of the standard Illumina sequencing primer and eliminating the need to design and optimize another sequencing primer for each new transposon sequence.

    Techniques: Selection, Sequencing

    Validation of RNA-Seq data by qRT–PCR. Nine PTI-, ETI-, and defence-related genes were selected for validation.

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: RNA-Seq Analysis of a Soybean Near-Isogenic Line Carrying Bacterial Leaf Pustule-Resistant and -Susceptible Alleles

    doi: 10.1093/dnares/dsr033

    Figure Lengend Snippet: Validation of RNA-Seq data by qRT–PCR. Nine PTI-, ETI-, and defence-related genes were selected for validation.

    Article Snippet: However, both of these methods rely primarily on existing expressed gene sequences for the synthesis of oligonucleotides and for generating qRT–PCR primers., Next-generation sequencing technologies, such as the widely used Illumina Genome Analyzer, , provide powerful alternative strategies for transcriptome analysis using direct mRNA-sequencing (RNA-Seq).

    Techniques: RNA Sequencing Assay, Quantitative RT-PCR

    Gene ontology (GO) classification for the P . delavayi transcriptome. The transcripts (33,249) were categorized into 55 function groups. The right y-axis indicates the number of genes in a category, whereas the left y-axis indicates the percentage of a specific category of genes in the corresponding main category.

    Journal: PLoS ONE

    Article Title: Transcriptomic Analysis of Paeonia delavayi Wild Population Flowers to Identify Differentially Expressed Genes Involved in Purple-Red and Yellow Petal Pigmentation

    doi: 10.1371/journal.pone.0135038

    Figure Lengend Snippet: Gene ontology (GO) classification for the P . delavayi transcriptome. The transcripts (33,249) were categorized into 55 function groups. The right y-axis indicates the number of genes in a category, whereas the left y-axis indicates the percentage of a specific category of genes in the corresponding main category.

    Article Snippet: In the present work, individuals with purple-red and yellow flowers within a wild P . delavayi population in Yunnan Province, China were used as experimental materials, and two transcriptomes (of petals of each color mixed at different flower opening stages) were sequenced on the Illumina HiSeq 2000 platform.

    Techniques: