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Roche primer combinations
Primer Combinations, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primer combinations/product/Roche
Average 91 stars, based on 7 article reviews
Price from $9.99 to $1999.99
primer combinations - by Bioz Stars, 2020-07
91/100 stars

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Related Articles

Multiplex Assay:

Article Title: Topoisomerase II? promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation
Article Snippet: .. DNA was purified (IPure kit; Diagenode with Precipitor; Abnova) and analysed by qPCR (QuantiFast Multiplex PCR Mix; Qiagen) in triplicates using primer combinations and probes covering regions of rDNA repeat ( ) on Light Cycler 480-II (Roche). ..

Amplification:

Article Title: Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines
Article Snippet: .. Then, only primer combinations whose products were close to the expected sizes were selected for amplification with the Expand High Fidelity PCR system (Roche, Germany). .. Next, the desired bands were recovered with a gel extraction kit (Qiagen, Germany) and TA cloned in pDrive vector (Qiagen, Germany).

Article Title: Origin, diversity, and maturation of human antiviral antibodies analyzed by high-throughput sequencing
Article Snippet: .. For 454 sequencing, primer combinations used to amplify cDNA in separate reactions included the Roche A and B adaptor sequences along with target amplification sequence for heavy and light chain variable domains. .. The gene fragments were amplified in 20 cycles of PCR using the High Fidelity PCR Master from Roche.

Article Title: Expressed antibody repertoires in human cord blood cells: 454 sequencing and IMGT/HighV-QUEST analysis of germline gene usage, junctional diversity, and somatic mutations
Article Snippet: .. For 454 sequencing, primer combinations used to amplify cDNA in separate reactions included the Roche A and B adaptor sequences along with target amplification sequence for heavy and light chain variable domains. .. For heavy chain, rHF5: 5′-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG ATC AGA CAC GTG GGG CGG ATG CAC TCCC-3′ (sense, for cord blood 1), rHF6: 5′-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG ATA TCG CGA GTG GGG CGG ATG CAC TCCC-3′ (sense, for cord blood 2), and rHR1: 5′-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG GCT GCC CAA CCA GCC ATG GCC-3′ (antisense, for both cord blood).

Article Title: A comprehensive approach to zinc-finger recombinase customization enables genomic targeting in human cells
Article Snippet: .. ZFR targets were PCR amplified with the following primer combinations: ZFR–Target-1, 2 or 3–Fwd and ZFR–Target-1, 2 or 3–Rev (Unmodified target); ZFR–Target-1, 2 or 3–Fwd and CMV–Mid–Prim-1 (Forward integration); and CMV–Mid–Prim-1 and ZFR–Target-1, 2 or 3–Rev (Reverse integration) using the Expand High Fidelity Taq System (Roche). ..

Purification:

Article Title: Topoisomerase II? promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation
Article Snippet: .. DNA was purified (IPure kit; Diagenode with Precipitor; Abnova) and analysed by qPCR (QuantiFast Multiplex PCR Mix; Qiagen) in triplicates using primer combinations and probes covering regions of rDNA repeat ( ) on Light Cycler 480-II (Roche). ..

Real-time Polymerase Chain Reaction:

Article Title: Topoisomerase II? promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation
Article Snippet: .. DNA was purified (IPure kit; Diagenode with Precipitor; Abnova) and analysed by qPCR (QuantiFast Multiplex PCR Mix; Qiagen) in triplicates using primer combinations and probes covering regions of rDNA repeat ( ) on Light Cycler 480-II (Roche). ..

Polymerase Chain Reaction:

Article Title: Molecular Characterization of the Carboxypeptidase B1 of Anopheles stephensi and Its Evaluation as a Target for Transmission-Blocking Vaccines
Article Snippet: .. Then, only primer combinations whose products were close to the expected sizes were selected for amplification with the Expand High Fidelity PCR system (Roche, Germany). .. Next, the desired bands were recovered with a gel extraction kit (Qiagen, Germany) and TA cloned in pDrive vector (Qiagen, Germany).

Article Title: SOX9 Duplication Linked to Intersex in Deer
Article Snippet: .. To investigate the localization and organization of the extra copy of the SOX9 gene, the respective regions were analyzed with several primer combinations by long-range PCR analyses using expand high fidelity PCR system (Roche). ..

Article Title: Fratricide activity of MafB protein of N. meningitidis strain B16B6
Article Snippet: .. PCRs were performed using 1–2 μl of extracted DNA, 200 μM dNTPs (Fermentas), 0.25 μM of different primer combinations (see in Additional file : Table S1), 0.5 U of Expand High Fidelity Enzyme Mix, and PCR buffer of the Expand High Fidelity PCR System (Roche). .. Thermal cycling conditions were 30 cycles of 1 min at 95 °C, 0.5 min at 58 °C and elongation at 72 °C during 1 min per kbp of expected amplicon size.

Article Title: Topoisomerase II? promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation
Article Snippet: .. DNA was purified (IPure kit; Diagenode with Precipitor; Abnova) and analysed by qPCR (QuantiFast Multiplex PCR Mix; Qiagen) in triplicates using primer combinations and probes covering regions of rDNA repeat ( ) on Light Cycler 480-II (Roche). ..

Article Title: A comprehensive approach to zinc-finger recombinase customization enables genomic targeting in human cells
Article Snippet: .. ZFR targets were PCR amplified with the following primer combinations: ZFR–Target-1, 2 or 3–Fwd and ZFR–Target-1, 2 or 3–Rev (Unmodified target); ZFR–Target-1, 2 or 3–Fwd and CMV–Mid–Prim-1 (Forward integration); and CMV–Mid–Prim-1 and ZFR–Target-1, 2 or 3–Rev (Reverse integration) using the Expand High Fidelity Taq System (Roche). ..

Sequencing:

Article Title: Origin, diversity, and maturation of human antiviral antibodies analyzed by high-throughput sequencing
Article Snippet: .. For 454 sequencing, primer combinations used to amplify cDNA in separate reactions included the Roche A and B adaptor sequences along with target amplification sequence for heavy and light chain variable domains. .. The gene fragments were amplified in 20 cycles of PCR using the High Fidelity PCR Master from Roche.

Article Title: Expressed antibody repertoires in human cord blood cells: 454 sequencing and IMGT/HighV-QUEST analysis of germline gene usage, junctional diversity, and somatic mutations
Article Snippet: .. For 454 sequencing, primer combinations used to amplify cDNA in separate reactions included the Roche A and B adaptor sequences along with target amplification sequence for heavy and light chain variable domains. .. For heavy chain, rHF5: 5′-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG ATC AGA CAC GTG GGG CGG ATG CAC TCCC-3′ (sense, for cord blood 1), rHF6: 5′-CCA TCT CAT CCC TGC GTG TCT CCG ACT CAG ATA TCG CGA GTG GGG CGG ATG CAC TCCC-3′ (sense, for cord blood 2), and rHR1: 5′-CCT ATC CCC TGT GTG CCT TGG CAG TCT CAG GCT GCC CAA CCA GCC ATG GCC-3′ (antisense, for both cord blood).

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  • 91
    Roche primer combination rps5a fw
    Genetic analysis of LCF1. ( a ) Southern blot on Nco I digested genomic DNA (gDNA) of empty vector control plants (Col-0 transformed with pRF-VP16-Kana not containing a 3F fragment; lane 2) and LCF1 (lane 3). Lane 1 contains DIG-labelled DNA ladder. The DIG-labelled probe was expected to hybridize with fragments of the promoter of the endogenous <t>RPS5a</t> gene (10695 bp fragment), the RPS5a promoter (p RPS5a ) from the T-DNA construct (2590 bp fragment with intensity depending on the copy number of the insert), and the (3F)-VP16 fusion encoding part of each T-DNA insert in the genome (fragments of ≥~1500 bp for LCF1 and ≥~800 bp for the empty vector control without 3F). ( b ) Map of the 3F-VP16 encoding T-DNA construct in MORC2 (At4g36280) of LCF1. MORC2 is presented to scale. Exons are presented as grey boxes. 5′ and 3′ untranslated regions (UTRs) are presented as white boxes. The T-DNA insert is not presented to scale. ( c ) RT-PCR analysis of MORC2 expression in Col-0 and LCF1 plants. The genes ATG6 (At3g61710) and SCAMP5 (At1g32050) serve as a controls for gene expression. Expression of the 3F-VP16 construct in LCF1 was verified as a positive control. ( d ) False color φPSII images of Col-0 and LCF1 plants (T1 generation; obtained through selection with PPT) harboring T-DNA constructs with either the GUS gene under control of the CaMV 35S promoter ( p35S::GUS ), the full length genomic sequence of MORC2 under control of its own promoter and terminator sequences (p MORC::MORC2 ) to assay for complementation, or the cDNA encoding MORC2 under control of the NOS promoter and terminator sequences ( pNOS::MORC2 ) to assay for complementation. The variation in size among the transformants was due to herbicide selection and subsequent transfer from selection medium to soil. ( e ) RT-qPCR analysis of the expression of the 5′ end of MORC2 ( MORC2Δ3 ′) and of the two neighboring genes At4g36270 and At4g36290 ( MORC1 ). Expression of the 3F-VP16 construct in LCF1 was quantified as a positive control. Relative expression values were obtained by normalizing to the expression values of the genes ATG6 and SCAMP5 . Error bars represent SEM values (n = 4). The presented data are the average of two technical replicates.
    Primer Combination Rps5a Fw, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer combination rps5a fw/product/Roche
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primer combination rps5a fw - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    91
    Roche primer probe combinations
    Genetic analysis of LCF1. ( a ) Southern blot on Nco I digested genomic DNA (gDNA) of empty vector control plants (Col-0 transformed with pRF-VP16-Kana not containing a 3F fragment; lane 2) and LCF1 (lane 3). Lane 1 contains DIG-labelled DNA ladder. The DIG-labelled probe was expected to hybridize with fragments of the promoter of the endogenous <t>RPS5a</t> gene (10695 bp fragment), the RPS5a promoter (p RPS5a ) from the T-DNA construct (2590 bp fragment with intensity depending on the copy number of the insert), and the (3F)-VP16 fusion encoding part of each T-DNA insert in the genome (fragments of ≥~1500 bp for LCF1 and ≥~800 bp for the empty vector control without 3F). ( b ) Map of the 3F-VP16 encoding T-DNA construct in MORC2 (At4g36280) of LCF1. MORC2 is presented to scale. Exons are presented as grey boxes. 5′ and 3′ untranslated regions (UTRs) are presented as white boxes. The T-DNA insert is not presented to scale. ( c ) RT-PCR analysis of MORC2 expression in Col-0 and LCF1 plants. The genes ATG6 (At3g61710) and SCAMP5 (At1g32050) serve as a controls for gene expression. Expression of the 3F-VP16 construct in LCF1 was verified as a positive control. ( d ) False color φPSII images of Col-0 and LCF1 plants (T1 generation; obtained through selection with PPT) harboring T-DNA constructs with either the GUS gene under control of the CaMV 35S promoter ( p35S::GUS ), the full length genomic sequence of MORC2 under control of its own promoter and terminator sequences (p MORC::MORC2 ) to assay for complementation, or the cDNA encoding MORC2 under control of the NOS promoter and terminator sequences ( pNOS::MORC2 ) to assay for complementation. The variation in size among the transformants was due to herbicide selection and subsequent transfer from selection medium to soil. ( e ) RT-qPCR analysis of the expression of the 5′ end of MORC2 ( MORC2Δ3 ′) and of the two neighboring genes At4g36270 and At4g36290 ( MORC1 ). Expression of the 3F-VP16 construct in LCF1 was quantified as a positive control. Relative expression values were obtained by normalizing to the expression values of the genes ATG6 and SCAMP5 . Error bars represent SEM values (n = 4). The presented data are the average of two technical replicates.
    Primer Probe Combinations, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer probe combinations/product/Roche
    Average 91 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    primer probe combinations - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    89
    Roche primer probe combination
    Genetic analysis of LCF1. ( a ) Southern blot on Nco I digested genomic DNA (gDNA) of empty vector control plants (Col-0 transformed with pRF-VP16-Kana not containing a 3F fragment; lane 2) and LCF1 (lane 3). Lane 1 contains DIG-labelled DNA ladder. The DIG-labelled probe was expected to hybridize with fragments of the promoter of the endogenous <t>RPS5a</t> gene (10695 bp fragment), the RPS5a promoter (p RPS5a ) from the T-DNA construct (2590 bp fragment with intensity depending on the copy number of the insert), and the (3F)-VP16 fusion encoding part of each T-DNA insert in the genome (fragments of ≥~1500 bp for LCF1 and ≥~800 bp for the empty vector control without 3F). ( b ) Map of the 3F-VP16 encoding T-DNA construct in MORC2 (At4g36280) of LCF1. MORC2 is presented to scale. Exons are presented as grey boxes. 5′ and 3′ untranslated regions (UTRs) are presented as white boxes. The T-DNA insert is not presented to scale. ( c ) RT-PCR analysis of MORC2 expression in Col-0 and LCF1 plants. The genes ATG6 (At3g61710) and SCAMP5 (At1g32050) serve as a controls for gene expression. Expression of the 3F-VP16 construct in LCF1 was verified as a positive control. ( d ) False color φPSII images of Col-0 and LCF1 plants (T1 generation; obtained through selection with PPT) harboring T-DNA constructs with either the GUS gene under control of the CaMV 35S promoter ( p35S::GUS ), the full length genomic sequence of MORC2 under control of its own promoter and terminator sequences (p MORC::MORC2 ) to assay for complementation, or the cDNA encoding MORC2 under control of the NOS promoter and terminator sequences ( pNOS::MORC2 ) to assay for complementation. The variation in size among the transformants was due to herbicide selection and subsequent transfer from selection medium to soil. ( e ) RT-qPCR analysis of the expression of the 5′ end of MORC2 ( MORC2Δ3 ′) and of the two neighboring genes At4g36270 and At4g36290 ( MORC1 ). Expression of the 3F-VP16 construct in LCF1 was quantified as a positive control. Relative expression values were obtained by normalizing to the expression values of the genes ATG6 and SCAMP5 . Error bars represent SEM values (n = 4). The presented data are the average of two technical replicates.
    Primer Probe Combination, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primer probe combination/product/Roche
    Average 89 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    primer probe combination - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    Image Search Results


    Genetic analysis of LCF1. ( a ) Southern blot on Nco I digested genomic DNA (gDNA) of empty vector control plants (Col-0 transformed with pRF-VP16-Kana not containing a 3F fragment; lane 2) and LCF1 (lane 3). Lane 1 contains DIG-labelled DNA ladder. The DIG-labelled probe was expected to hybridize with fragments of the promoter of the endogenous RPS5a gene (10695 bp fragment), the RPS5a promoter (p RPS5a ) from the T-DNA construct (2590 bp fragment with intensity depending on the copy number of the insert), and the (3F)-VP16 fusion encoding part of each T-DNA insert in the genome (fragments of ≥~1500 bp for LCF1 and ≥~800 bp for the empty vector control without 3F). ( b ) Map of the 3F-VP16 encoding T-DNA construct in MORC2 (At4g36280) of LCF1. MORC2 is presented to scale. Exons are presented as grey boxes. 5′ and 3′ untranslated regions (UTRs) are presented as white boxes. The T-DNA insert is not presented to scale. ( c ) RT-PCR analysis of MORC2 expression in Col-0 and LCF1 plants. The genes ATG6 (At3g61710) and SCAMP5 (At1g32050) serve as a controls for gene expression. Expression of the 3F-VP16 construct in LCF1 was verified as a positive control. ( d ) False color φPSII images of Col-0 and LCF1 plants (T1 generation; obtained through selection with PPT) harboring T-DNA constructs with either the GUS gene under control of the CaMV 35S promoter ( p35S::GUS ), the full length genomic sequence of MORC2 under control of its own promoter and terminator sequences (p MORC::MORC2 ) to assay for complementation, or the cDNA encoding MORC2 under control of the NOS promoter and terminator sequences ( pNOS::MORC2 ) to assay for complementation. The variation in size among the transformants was due to herbicide selection and subsequent transfer from selection medium to soil. ( e ) RT-qPCR analysis of the expression of the 5′ end of MORC2 ( MORC2Δ3 ′) and of the two neighboring genes At4g36270 and At4g36290 ( MORC1 ). Expression of the 3F-VP16 construct in LCF1 was quantified as a positive control. Relative expression values were obtained by normalizing to the expression values of the genes ATG6 and SCAMP5 . Error bars represent SEM values (n = 4). The presented data are the average of two technical replicates.

    Journal: Scientific Reports

    Article Title: An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence

    doi: 10.1038/s41598-017-03611-1

    Figure Lengend Snippet: Genetic analysis of LCF1. ( a ) Southern blot on Nco I digested genomic DNA (gDNA) of empty vector control plants (Col-0 transformed with pRF-VP16-Kana not containing a 3F fragment; lane 2) and LCF1 (lane 3). Lane 1 contains DIG-labelled DNA ladder. The DIG-labelled probe was expected to hybridize with fragments of the promoter of the endogenous RPS5a gene (10695 bp fragment), the RPS5a promoter (p RPS5a ) from the T-DNA construct (2590 bp fragment with intensity depending on the copy number of the insert), and the (3F)-VP16 fusion encoding part of each T-DNA insert in the genome (fragments of ≥~1500 bp for LCF1 and ≥~800 bp for the empty vector control without 3F). ( b ) Map of the 3F-VP16 encoding T-DNA construct in MORC2 (At4g36280) of LCF1. MORC2 is presented to scale. Exons are presented as grey boxes. 5′ and 3′ untranslated regions (UTRs) are presented as white boxes. The T-DNA insert is not presented to scale. ( c ) RT-PCR analysis of MORC2 expression in Col-0 and LCF1 plants. The genes ATG6 (At3g61710) and SCAMP5 (At1g32050) serve as a controls for gene expression. Expression of the 3F-VP16 construct in LCF1 was verified as a positive control. ( d ) False color φPSII images of Col-0 and LCF1 plants (T1 generation; obtained through selection with PPT) harboring T-DNA constructs with either the GUS gene under control of the CaMV 35S promoter ( p35S::GUS ), the full length genomic sequence of MORC2 under control of its own promoter and terminator sequences (p MORC::MORC2 ) to assay for complementation, or the cDNA encoding MORC2 under control of the NOS promoter and terminator sequences ( pNOS::MORC2 ) to assay for complementation. The variation in size among the transformants was due to herbicide selection and subsequent transfer from selection medium to soil. ( e ) RT-qPCR analysis of the expression of the 5′ end of MORC2 ( MORC2Δ3 ′) and of the two neighboring genes At4g36270 and At4g36290 ( MORC1 ). Expression of the 3F-VP16 construct in LCF1 was quantified as a positive control. Relative expression values were obtained by normalizing to the expression values of the genes ATG6 and SCAMP5 . Error bars represent SEM values (n = 4). The presented data are the average of two technical replicates.

    Article Snippet: As a probe, DIG-labelled PCR product was generated with the primer combination RPS5a FW and tNOS REV (Table ) from LCF genomic DNA, using PCR DIG Labelling Mix (Roche).

    Techniques: Southern Blot, Plasmid Preparation, Transformation Assay, Construct, Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control, Selection, Sequencing, Quantitative RT-PCR