Journal: bioRxiv
Article Title: Cytochrome P450 inhibition impedes pyrethroid effects on insects through Nav channel regulation
doi: 10.1101/2025.02.03.636193
Figure Lengend Snippet: a,d. Light photomicrographs of isolated adult DUM neurons. b-f. Isolated DUM neuron cell bodies labeled with anti-SP19 (Nav) antibody, which was detected with FITC conjugated secondary antibody (positive green fluorescence, white arrows) ( b,e ) and anti-Cyp4A11 antibody detected with Alexa Fluor 633 conjugated secondary antibody (positive red fluorescence, white arrows) (c,f). Both positive staining and co-localization of the two proteins present a granular appearance and is most intense in the same basal region of the soma close to the initial segment (open circles). Negative control experiments showing the specificity of the primary antibodies binding to the sodium channels and the Cyp4A11, together with the secondary antibody controls that show that the label is specific to the primary antibodies are illustrated in Supplementary Information .
Article Snippet: To block non-specific binding of the primary antibodies, cell bodies were preincubated with 4% bovine serum albumin (BSA) in PBS-T for 1 h. Primary antisera rabbit polyclonal anti-voltage-gated sodium channel (SP19 Segment) antibody (Sigma Chemicals, L’isle d’Abeau Chesnes, France) or mouse monoclonal anti-Cyp4A11 (Bio-Techne Ltd., Abingdon, UK), both diluted 1/100 in PBS-T, were applied overnight at 4°C.
Techniques: Isolation, Labeling, Fluorescence, Staining, Negative Control, Binding Assay