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primary rabbit polyclonal antibodies  (Danaher Inc)


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    Structured Review

    Danaher Inc primary rabbit polyclonal antibodies
    Primary Rabbit Polyclonal Antibodies, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary rabbit polyclonal antibodies/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    primary rabbit polyclonal antibodies - by Bioz Stars, 2025-02
    86/100 stars

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    Millipore ab 94882 millipore tris edta iba 1 ihc rabbit polyclonal primary
    a,d. Light photomicrographs of isolated adult DUM neurons. b-f. Isolated DUM neuron cell bodies labeled with <t>anti-SP19</t> (Nav) antibody, which was detected with FITC conjugated secondary antibody (positive green fluorescence, white arrows) ( b,e ) and anti-Cyp4A11 antibody detected with Alexa Fluor 633 conjugated secondary antibody (positive red fluorescence, white arrows) (c,f). Both positive staining and co-localization of the two proteins present a granular appearance and is most intense in the same basal region of the soma close to the initial segment (open circles). Negative control experiments showing the specificity of the primary antibodies binding to the sodium channels and the Cyp4A11, together with the secondary antibody controls that show that the label is specific to the primary antibodies are illustrated in Supplementary Information .
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    a,d. Light photomicrographs of isolated adult DUM neurons. b-f. Isolated DUM neuron cell bodies labeled with anti-SP19 (Nav) antibody, which was detected with FITC conjugated secondary antibody (positive green fluorescence, white arrows) ( b,e ) and anti-Cyp4A11 antibody detected with Alexa Fluor 633 conjugated secondary antibody (positive red fluorescence, white arrows) (c,f). Both positive staining and co-localization of the two proteins present a granular appearance and is most intense in the same basal region of the soma close to the initial segment (open circles). Negative control experiments showing the specificity of the primary antibodies binding to the sodium channels and the Cyp4A11, together with the secondary antibody controls that show that the label is specific to the primary antibodies are illustrated in Supplementary Information .

    Journal: bioRxiv

    Article Title: Cytochrome P450 inhibition impedes pyrethroid effects on insects through Nav channel regulation

    doi: 10.1101/2025.02.03.636193

    Figure Lengend Snippet: a,d. Light photomicrographs of isolated adult DUM neurons. b-f. Isolated DUM neuron cell bodies labeled with anti-SP19 (Nav) antibody, which was detected with FITC conjugated secondary antibody (positive green fluorescence, white arrows) ( b,e ) and anti-Cyp4A11 antibody detected with Alexa Fluor 633 conjugated secondary antibody (positive red fluorescence, white arrows) (c,f). Both positive staining and co-localization of the two proteins present a granular appearance and is most intense in the same basal region of the soma close to the initial segment (open circles). Negative control experiments showing the specificity of the primary antibodies binding to the sodium channels and the Cyp4A11, together with the secondary antibody controls that show that the label is specific to the primary antibodies are illustrated in Supplementary Information .

    Article Snippet: To block non-specific binding of the primary antibodies, cell bodies were preincubated with 4% bovine serum albumin (BSA) in PBS-T for 1 h. Primary antisera rabbit polyclonal anti-voltage-gated sodium channel (SP19 Segment) antibody (Sigma Chemicals, L’isle d’Abeau Chesnes, France) or mouse monoclonal anti-Cyp4A11 (Bio-Techne Ltd., Abingdon, UK), both diluted 1/100 in PBS-T, were applied overnight at 4°C.

    Techniques: Isolation, Labeling, Fluorescence, Staining, Negative Control, Binding Assay

    a-c . Light photomicrograph of isolated DUM neuron cell bodies (a), treated with anti-SP19 (Nav) antibody were incubated with Alexa Fluor 633 conjugated secondary antibody and recorded with the wavelength of excitation of FITC (477 nm), ( b ) and the wavelength of excitation of Alexa Fluor 633 (633 nm), ( c ). d-f. Isolated DUM neuron cell body (light photomicrograph ( d ), treated with anti-Cyp4A11 antibody were incubated with FITC conjugated secondary antibody and recorded with the wavelength of excitation of FITC (477 nm, e ) and the wavelength of excitation of Alexa Fluor 633 (638 nm, f ).

    Journal: bioRxiv

    Article Title: Cytochrome P450 inhibition impedes pyrethroid effects on insects through Nav channel regulation

    doi: 10.1101/2025.02.03.636193

    Figure Lengend Snippet: a-c . Light photomicrograph of isolated DUM neuron cell bodies (a), treated with anti-SP19 (Nav) antibody were incubated with Alexa Fluor 633 conjugated secondary antibody and recorded with the wavelength of excitation of FITC (477 nm), ( b ) and the wavelength of excitation of Alexa Fluor 633 (633 nm), ( c ). d-f. Isolated DUM neuron cell body (light photomicrograph ( d ), treated with anti-Cyp4A11 antibody were incubated with FITC conjugated secondary antibody and recorded with the wavelength of excitation of FITC (477 nm, e ) and the wavelength of excitation of Alexa Fluor 633 (638 nm, f ).

    Article Snippet: To block non-specific binding of the primary antibodies, cell bodies were preincubated with 4% bovine serum albumin (BSA) in PBS-T for 1 h. Primary antisera rabbit polyclonal anti-voltage-gated sodium channel (SP19 Segment) antibody (Sigma Chemicals, L’isle d’Abeau Chesnes, France) or mouse monoclonal anti-Cyp4A11 (Bio-Techne Ltd., Abingdon, UK), both diluted 1/100 in PBS-T, were applied overnight at 4°C.

    Techniques: Isolation, Incubation