primary human dermal fibroblasts phdf  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained <t>pHDF</t> cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers"

    Article Title: Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers

    Journal: ACS Applied Materials & Interfaces

    doi: 10.1021/acsami.1c17901

    Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained pHDF cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.
    Figure Legend Snippet: Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained pHDF cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.

    Techniques Used: Confocal Microscopy, Staining, Microscopy, Epifluorescence Microscopy

    Applicability of patterned poly(acrylamide) hydrogels for traction force microscopy applications. (A) Confocal microscopy image of fluorescent beads on the upper layer of the hydrogel, merged with the transmission image showing a live pHDF fibroblast adhering on a line pattern (schematically overlaid in gray). The hydrogel used had a Young’s modulus of 2 kPa and was functionalized with α 5 β 1 integrin-selective peptidomimetics. (B) Particle image velocimetry (PIV) analysis results from bead displacements observed before and after the removal of the cell shown in (A). The magnitude of the color-coded vectors is given in pixels. (C) Confocal microscopy image of a patterned and AlexaFluor 568-labeled hydrogel. A square grid pattern of 1 μm circles spaced 3 μm apart was used as the virtual mask. (D) Merged confocal microscopy image of a live REF YFP-PAX cell with a fluorescent pattern on a 7 kPa hydrogel. (E) Substrate deformation field calculated from PIV analysis of panel (D). Cell outlines (white) in (D, E) are a guide to the eye. Scale bar: 20 μm; inset: 5 μm.
    Figure Legend Snippet: Applicability of patterned poly(acrylamide) hydrogels for traction force microscopy applications. (A) Confocal microscopy image of fluorescent beads on the upper layer of the hydrogel, merged with the transmission image showing a live pHDF fibroblast adhering on a line pattern (schematically overlaid in gray). The hydrogel used had a Young’s modulus of 2 kPa and was functionalized with α 5 β 1 integrin-selective peptidomimetics. (B) Particle image velocimetry (PIV) analysis results from bead displacements observed before and after the removal of the cell shown in (A). The magnitude of the color-coded vectors is given in pixels. (C) Confocal microscopy image of a patterned and AlexaFluor 568-labeled hydrogel. A square grid pattern of 1 μm circles spaced 3 μm apart was used as the virtual mask. (D) Merged confocal microscopy image of a live REF YFP-PAX cell with a fluorescent pattern on a 7 kPa hydrogel. (E) Substrate deformation field calculated from PIV analysis of panel (D). Cell outlines (white) in (D, E) are a guide to the eye. Scale bar: 20 μm; inset: 5 μm.

    Techniques Used: Microscopy, Confocal Microscopy, Transmission Assay, Labeling

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    86/100 stars

    Images

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    86/100 stars

    Images

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    86/100 stars

    Images

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained <t>pHDF</t> cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers"

    Article Title: Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers

    Journal: ACS Applied Materials & Interfaces

    doi: 10.1021/acsami.1c17901

    Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained pHDF cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.
    Figure Legend Snippet: Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained pHDF cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.

    Techniques Used: Confocal Microscopy, Staining, Microscopy, Epifluorescence Microscopy

    Applicability of patterned poly(acrylamide) hydrogels for traction force microscopy applications. (A) Confocal microscopy image of fluorescent beads on the upper layer of the hydrogel, merged with the transmission image showing a live pHDF fibroblast adhering on a line pattern (schematically overlaid in gray). The hydrogel used had a Young’s modulus of 2 kPa and was functionalized with α 5 β 1 integrin-selective peptidomimetics. (B) Particle image velocimetry (PIV) analysis results from bead displacements observed before and after the removal of the cell shown in (A). The magnitude of the color-coded vectors is given in pixels. (C) Confocal microscopy image of a patterned and AlexaFluor 568-labeled hydrogel. A square grid pattern of 1 μm circles spaced 3 μm apart was used as the virtual mask. (D) Merged confocal microscopy image of a live REF YFP-PAX cell with a fluorescent pattern on a 7 kPa hydrogel. (E) Substrate deformation field calculated from PIV analysis of panel (D). Cell outlines (white) in (D, E) are a guide to the eye. Scale bar: 20 μm; inset: 5 μm.
    Figure Legend Snippet: Applicability of patterned poly(acrylamide) hydrogels for traction force microscopy applications. (A) Confocal microscopy image of fluorescent beads on the upper layer of the hydrogel, merged with the transmission image showing a live pHDF fibroblast adhering on a line pattern (schematically overlaid in gray). The hydrogel used had a Young’s modulus of 2 kPa and was functionalized with α 5 β 1 integrin-selective peptidomimetics. (B) Particle image velocimetry (PIV) analysis results from bead displacements observed before and after the removal of the cell shown in (A). The magnitude of the color-coded vectors is given in pixels. (C) Confocal microscopy image of a patterned and AlexaFluor 568-labeled hydrogel. A square grid pattern of 1 μm circles spaced 3 μm apart was used as the virtual mask. (D) Merged confocal microscopy image of a live REF YFP-PAX cell with a fluorescent pattern on a 7 kPa hydrogel. (E) Substrate deformation field calculated from PIV analysis of panel (D). Cell outlines (white) in (D, E) are a guide to the eye. Scale bar: 20 μm; inset: 5 μm.

    Techniques Used: Microscopy, Confocal Microscopy, Transmission Assay, Labeling

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    93/100 stars

    Images

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    A) Epifluorescence microscopy images of Phalloidin-stained <t>pHDF</t> seeded for 3 hours on soft elastomers (ζ=0.7), treated with 5 μM blebbistatin or equivalent volume of carrier (DMSO). Nuclei are stained with DAPI (blue color). B) Confocal microscopy images of fixed and immunostained pHDF seeded on soft, FN-coated elastomers (ζ=0.7), treated with blebbistatin or DMSO. Scale bars: 20 μm/5 μm (details). Quantification of C) cell area, D) aspect ratio and E) FA area of <t>pHDF</t> <t>cultured</t> for 3 hours on soft elastomers (ζ=0.7), treated with 5 μM blebbistatin or equivalent volume of carrier (DMSO). Each data point represents the mean value of an independent experiment. F) Typical PIV analysis of bead displacements on an elastomer seeded with blebbistatin-treated pHDF for 1 hour. The magnitude of the color-coded vectors is given in pixels and is the same as for Figure 5E for comparison; the white line denotes the cell outline. Scale bar: 20 μm. G) Deformation as a function of distance from the cell center for blebbistatin-treated pHDF cells. The data (mean ± SD, n=8) were fitted using an exponential decay function (red line) starting from a distance of 20 μm, which corresponds to the typical cell radius. One of two independent experiments is presented. G) Quantification of the boundary distance and AUC for the situation of blebbistatin-treated cells. Comparison with the mean value calculated for control cells (dashed line; data from figure 5G) shows how contractility inhibition lowers substrate deformations.
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    99/100 stars

    Images

    1) Product Images from "Substrate Resistance to Traction Forces Controls Fibroblast Polarization"

    Article Title: Substrate Resistance to Traction Forces Controls Fibroblast Polarization

    Journal: bioRxiv

    doi: 10.1101/2020.05.15.098046

    A) Epifluorescence microscopy images of Phalloidin-stained pHDF seeded for 3 hours on soft elastomers (ζ=0.7), treated with 5 μM blebbistatin or equivalent volume of carrier (DMSO). Nuclei are stained with DAPI (blue color). B) Confocal microscopy images of fixed and immunostained pHDF seeded on soft, FN-coated elastomers (ζ=0.7), treated with blebbistatin or DMSO. Scale bars: 20 μm/5 μm (details). Quantification of C) cell area, D) aspect ratio and E) FA area of pHDF cultured for 3 hours on soft elastomers (ζ=0.7), treated with 5 μM blebbistatin or equivalent volume of carrier (DMSO). Each data point represents the mean value of an independent experiment. F) Typical PIV analysis of bead displacements on an elastomer seeded with blebbistatin-treated pHDF for 1 hour. The magnitude of the color-coded vectors is given in pixels and is the same as for Figure 5E for comparison; the white line denotes the cell outline. Scale bar: 20 μm. G) Deformation as a function of distance from the cell center for blebbistatin-treated pHDF cells. The data (mean ± SD, n=8) were fitted using an exponential decay function (red line) starting from a distance of 20 μm, which corresponds to the typical cell radius. One of two independent experiments is presented. G) Quantification of the boundary distance and AUC for the situation of blebbistatin-treated cells. Comparison with the mean value calculated for control cells (dashed line; data from figure 5G) shows how contractility inhibition lowers substrate deformations.
    Figure Legend Snippet: A) Epifluorescence microscopy images of Phalloidin-stained pHDF seeded for 3 hours on soft elastomers (ζ=0.7), treated with 5 μM blebbistatin or equivalent volume of carrier (DMSO). Nuclei are stained with DAPI (blue color). B) Confocal microscopy images of fixed and immunostained pHDF seeded on soft, FN-coated elastomers (ζ=0.7), treated with blebbistatin or DMSO. Scale bars: 20 μm/5 μm (details). Quantification of C) cell area, D) aspect ratio and E) FA area of pHDF cultured for 3 hours on soft elastomers (ζ=0.7), treated with 5 μM blebbistatin or equivalent volume of carrier (DMSO). Each data point represents the mean value of an independent experiment. F) Typical PIV analysis of bead displacements on an elastomer seeded with blebbistatin-treated pHDF for 1 hour. The magnitude of the color-coded vectors is given in pixels and is the same as for Figure 5E for comparison; the white line denotes the cell outline. Scale bar: 20 μm. G) Deformation as a function of distance from the cell center for blebbistatin-treated pHDF cells. The data (mean ± SD, n=8) were fitted using an exponential decay function (red line) starting from a distance of 20 μm, which corresponds to the typical cell radius. One of two independent experiments is presented. G) Quantification of the boundary distance and AUC for the situation of blebbistatin-treated cells. Comparison with the mean value calculated for control cells (dashed line; data from figure 5G) shows how contractility inhibition lowers substrate deformations.

    Techniques Used: Epifluorescence Microscopy, Staining, Confocal Microscopy, Cell Culture, Inhibition

    primary human dermal fibroblasts phdf  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
    Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    ATCC primary human dermal fibroblasts phdf
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    99/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    ATCC primary human dermal fibroblasts phdf
    Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained <t>pHDF</t> cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.
    Primary Human Dermal Fibroblasts Phdf, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human dermal fibroblasts phdf/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary human dermal fibroblasts phdf - by Bioz Stars, 2024-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained pHDF cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers

    doi: 10.1021/acsami.1c17901

    Figure Lengend Snippet: Cell patterning on poly(acrylamide) hydrogels. Line patterns were prepared on 5% Am/0.1% Bis pAAm hydrogels incorporating 3 mM caged AEMA. Patterns were functionalized with indicated integrin ligands. Primary human dermal fibroblast adhered selectively to patterns of immobilized cyclic RGDfK peptides (A) and α 5 β 1 integrin-selective integrin peptidomimetics (B). (A) Confocal microscopy image of fixed and phalloidin-stained pHDF cells. Phalloidin stains filamentous actin; nuclei are stained in blue. (B) Phase contrast microscopy image of live pHDF cells on hydrogel patterned with α 5 β 1 integrin-selective peptidomimetics. (C) CHO-A5 cells adhered to patterned α IIb β 3 integrin-selective ligands as exemplified with the epifluorescence microscopy image of phalloidin-stained cells presented (nuclei stained in blue). (D) Phase contrast microscopy image of live pHDF recognizing line patterns of fibronectin immobilized through biotin–streptavidin chemistry. Scale bar (A,D): 100 μm, B: 75 μm, (C): 50 μm.

    Article Snippet: Primary human dermal fibroblasts (pHDF) were purchased from ATCC (Cat #PCS-201-010).

    Techniques: Confocal Microscopy, Staining, Microscopy, Epifluorescence Microscopy

    Applicability of patterned poly(acrylamide) hydrogels for traction force microscopy applications. (A) Confocal microscopy image of fluorescent beads on the upper layer of the hydrogel, merged with the transmission image showing a live pHDF fibroblast adhering on a line pattern (schematically overlaid in gray). The hydrogel used had a Young’s modulus of 2 kPa and was functionalized with α 5 β 1 integrin-selective peptidomimetics. (B) Particle image velocimetry (PIV) analysis results from bead displacements observed before and after the removal of the cell shown in (A). The magnitude of the color-coded vectors is given in pixels. (C) Confocal microscopy image of a patterned and AlexaFluor 568-labeled hydrogel. A square grid pattern of 1 μm circles spaced 3 μm apart was used as the virtual mask. (D) Merged confocal microscopy image of a live REF YFP-PAX cell with a fluorescent pattern on a 7 kPa hydrogel. (E) Substrate deformation field calculated from PIV analysis of panel (D). Cell outlines (white) in (D, E) are a guide to the eye. Scale bar: 20 μm; inset: 5 μm.

    Journal: ACS Applied Materials & Interfaces

    Article Title: Facile and Versatile Method for Micropatterning Poly(acrylamide) Hydrogels Using Photocleavable Comonomers

    doi: 10.1021/acsami.1c17901

    Figure Lengend Snippet: Applicability of patterned poly(acrylamide) hydrogels for traction force microscopy applications. (A) Confocal microscopy image of fluorescent beads on the upper layer of the hydrogel, merged with the transmission image showing a live pHDF fibroblast adhering on a line pattern (schematically overlaid in gray). The hydrogel used had a Young’s modulus of 2 kPa and was functionalized with α 5 β 1 integrin-selective peptidomimetics. (B) Particle image velocimetry (PIV) analysis results from bead displacements observed before and after the removal of the cell shown in (A). The magnitude of the color-coded vectors is given in pixels. (C) Confocal microscopy image of a patterned and AlexaFluor 568-labeled hydrogel. A square grid pattern of 1 μm circles spaced 3 μm apart was used as the virtual mask. (D) Merged confocal microscopy image of a live REF YFP-PAX cell with a fluorescent pattern on a 7 kPa hydrogel. (E) Substrate deformation field calculated from PIV analysis of panel (D). Cell outlines (white) in (D, E) are a guide to the eye. Scale bar: 20 μm; inset: 5 μm.

    Article Snippet: Primary human dermal fibroblasts (pHDF) were purchased from ATCC (Cat #PCS-201-010).

    Techniques: Microscopy, Confocal Microscopy, Transmission Assay, Labeling