primary drg cultures  (Roche)


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    Roche primary drg cultures
    MOR agonists increase MMP-9 expression in dissociated neurons of <t>DRG</t> cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P
    Primary Drg Cultures, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary drg cultures/product/Roche
    Average 85 stars, based on 2 article reviews
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    primary drg cultures - by Bioz Stars, 2020-09
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    Images

    1) Product Images from "Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice"

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    Journal: Molecular Pain

    doi: 10.1186/1744-8069-8-19

    MOR agonists increase MMP-9 expression in dissociated neurons of DRG cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P
    Figure Legend Snippet: MOR agonists increase MMP-9 expression in dissociated neurons of DRG cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P

    Techniques Used: Expressing, Staining, Marker, Double Staining

    Up-regulation of MMP-9 expression in DRGs but not spinal cords after intrathecal morphine or remifentanil and potentiation of intrathecal morphine analgesia in Mmp9 deficient mice . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) 2 h after intrathecal injection of morphine (10 nmol) or remifentanil (1 nmol). B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P
    Figure Legend Snippet: Up-regulation of MMP-9 expression in DRGs but not spinal cords after intrathecal morphine or remifentanil and potentiation of intrathecal morphine analgesia in Mmp9 deficient mice . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) 2 h after intrathecal injection of morphine (10 nmol) or remifentanil (1 nmol). B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P

    Techniques Used: Expressing, Mouse Assay, Western Blot, Injection

    Intrathecal administration of MMP-9 siRNA reduces MMP-9 expression in DRGs and enhances morphine analgesia . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) after intrathecal injections of MMP-9 siRNA or control siRNA (3 μg, 24 and 2 h before the s.c. morphine injection. B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P
    Figure Legend Snippet: Intrathecal administration of MMP-9 siRNA reduces MMP-9 expression in DRGs and enhances morphine analgesia . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) after intrathecal injections of MMP-9 siRNA or control siRNA (3 μg, 24 and 2 h before the s.c. morphine injection. B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P

    Techniques Used: Expressing, Western Blot, Injection

    MMP-9 deficiency does not alter opioid-induced hyperalgesia after subcutaneous morphine . (A, B) Western blot gel (A) and band density (B) showing MMP-9 expression in lumbar DRGs 24 h after morphine (MORPH) injection. * P > 0.05, compared to naive control (CTRL), Student's t-test, n = 4 mice. (C, D) Subcutaneous morphine (5 mg/kg) induces comparable heat hyperalgesia in both wild-type and Mmp9 -KO mice (C) and also in wild-type mice pretreated with intrathecal MMP-9 inhibitor-I (0.2 μg) or vehicle (10% DMSO). * P
    Figure Legend Snippet: MMP-9 deficiency does not alter opioid-induced hyperalgesia after subcutaneous morphine . (A, B) Western blot gel (A) and band density (B) showing MMP-9 expression in lumbar DRGs 24 h after morphine (MORPH) injection. * P > 0.05, compared to naive control (CTRL), Student's t-test, n = 4 mice. (C, D) Subcutaneous morphine (5 mg/kg) induces comparable heat hyperalgesia in both wild-type and Mmp9 -KO mice (C) and also in wild-type mice pretreated with intrathecal MMP-9 inhibitor-I (0.2 μg) or vehicle (10% DMSO). * P

    Techniques Used: Western Blot, Expressing, Injection, Mouse Assay

    Subcutaneous morphine induces MMP-9 and TIMP-1 mRNA up-regulation in DRGs . (A, B) RT-PCR showing time course of subcutaneous morphine-induced MMP-9 (A) and TIMP-1 (B) mRNA expression in DRGs. * P
    Figure Legend Snippet: Subcutaneous morphine induces MMP-9 and TIMP-1 mRNA up-regulation in DRGs . (A, B) RT-PCR showing time course of subcutaneous morphine-induced MMP-9 (A) and TIMP-1 (B) mRNA expression in DRGs. * P

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Expressing

    Subcutaneous morphine-induced MMP-9 increase is enriched in mu opioid receptor (MOR)-expressing DRG neurons . (A, B) Double staining showing colocalization of MMP-9 and MOR in DRG neurons of saline (A) and morphine (B) treated mice (s.c., 10 mg/kg). Scale, 50 μm. (C, D) Percentage of MMP-9+ (C) and MOR + (D) neurons in DRGs 2 h after saline and morphine injection. (E) Percentage of MMP-9 and MOR double-positive neurons in DRGs 2 h after saline and morphine injection. (F) Percentage of MMP-9-positive neurons expressing MOR and percentage of MOR-positive neurons expressing MMP-9 in DRGs 2 h after saline and morphine injection. S, saline; M, morphine. * P
    Figure Legend Snippet: Subcutaneous morphine-induced MMP-9 increase is enriched in mu opioid receptor (MOR)-expressing DRG neurons . (A, B) Double staining showing colocalization of MMP-9 and MOR in DRG neurons of saline (A) and morphine (B) treated mice (s.c., 10 mg/kg). Scale, 50 μm. (C, D) Percentage of MMP-9+ (C) and MOR + (D) neurons in DRGs 2 h after saline and morphine injection. (E) Percentage of MMP-9 and MOR double-positive neurons in DRGs 2 h after saline and morphine injection. (F) Percentage of MMP-9-positive neurons expressing MOR and percentage of MOR-positive neurons expressing MMP-9 in DRGs 2 h after saline and morphine injection. S, saline; M, morphine. * P

    Techniques Used: Expressing, Double Staining, Mouse Assay, Injection

    Actue morphine treatment does not change the sizes of MMP-9- and MOR-positive neurons in DRGs . (A, B) Size frequency distribution (percentage) of MMP-9-positive neurons (A) and MOR-positive neurons (B) in DRGs 2 h after subcutaneous saline and morphine. 300-500 neurons from 3 animals were plotted in each group. Note that MMP-9 and MOR are mainly expressed in small- and medium-sized neurons.
    Figure Legend Snippet: Actue morphine treatment does not change the sizes of MMP-9- and MOR-positive neurons in DRGs . (A, B) Size frequency distribution (percentage) of MMP-9-positive neurons (A) and MOR-positive neurons (B) in DRGs 2 h after subcutaneous saline and morphine. 300-500 neurons from 3 animals were plotted in each group. Note that MMP-9 and MOR are mainly expressed in small- and medium-sized neurons.

    Techniques Used:

    Subcutaneous morphine increases MMP-9 expression and activity in DRGs . (A) Western blotting showing the time course of MMP-9 expression in lumbar DRGs after morphine (s.c., 10 mg/kg). (B) Gelatin zymography showing the time course of MMP-9 activity in lumbar DRGs after morphine. Note that MMP-2 activity remains unchanged after morphine injection. (C) Western blotting showing the time course of MMP-9 expression in lumbar spinal cord dorsal horns after morphine (s.c., 10 mg/kg). Note that subcutaneous morphine only induces MMP-9 expression in DRGs but not spinal cords. * P
    Figure Legend Snippet: Subcutaneous morphine increases MMP-9 expression and activity in DRGs . (A) Western blotting showing the time course of MMP-9 expression in lumbar DRGs after morphine (s.c., 10 mg/kg). (B) Gelatin zymography showing the time course of MMP-9 activity in lumbar DRGs after morphine. Note that MMP-2 activity remains unchanged after morphine injection. (C) Western blotting showing the time course of MMP-9 expression in lumbar spinal cord dorsal horns after morphine (s.c., 10 mg/kg). Note that subcutaneous morphine only induces MMP-9 expression in DRGs but not spinal cords. * P

    Techniques Used: Expressing, Activity Assay, Western Blot, Zymography, Injection

    Related Articles

    Incubation:

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice
    Article Snippet: .. Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma). .. Cells were then mechanically dissociated with a flame polished Pasteur pipette in the presence of 0.05% DNAse I (Sigma).

    Immunocytochemistry:

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice
    Article Snippet: .. Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma). .. Cells were then mechanically dissociated with a flame polished Pasteur pipette in the presence of 0.05% DNAse I (Sigma).

    Mouse Assay:

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice
    Article Snippet: .. Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma). .. Cells were then mechanically dissociated with a flame polished Pasteur pipette in the presence of 0.05% DNAse I (Sigma).

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    Roche primary drg cultures
    MOR agonists increase MMP-9 expression in dissociated neurons of <t>DRG</t> cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P
    Primary Drg Cultures, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary drg cultures/product/Roche
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    primary drg cultures - by Bioz Stars, 2020-09
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    Roche primary drg neuron culture drgs
    MOR agonists increase MMP-9 expression in dissociated neurons of <t>DRG</t> cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P
    Primary Drg Neuron Culture Drgs, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary drg neuron culture drgs/product/Roche
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Roche drg neuron enriched cultures
    MOR agonists increase MMP-9 expression in dissociated neurons of <t>DRG</t> cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P
    Drg Neuron Enriched Cultures, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/drg neuron enriched cultures/product/Roche
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    drg neuron enriched cultures - by Bioz Stars, 2020-09
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    MOR agonists increase MMP-9 expression in dissociated neurons of DRG cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: MOR agonists increase MMP-9 expression in dissociated neurons of DRG cultures . (A) Triple staining of MMP-9, NeuN (neuronal marker), and DAPI (nuclei marker for all cells). Note that MMP-9 is only expressed in neurons (arrows). Scale, 100 μm. (B) Double staining showing MMP-9 and NeuN expression in dissociated DRG neurons in control cultures and cultures treated with morphine (10 μM, 2 h). Left panels show all neurons (NeuN+) in the field. Right panels show high magnification images of middle panels. Scales, 100 μm (middle) and 25 μm (right). (C) Intensity of MMP-9-positive neurons in DRG cultures after treatment of morphine (1, 10, and 50 μM, 2 h), DAMGO and remifentanil (1 and 10 μM, 2 h), together with the opioid receptor antagonist naloxone and MOR antagonist CTAT (10 and 50 μM). Note that morphine-induced MMP-9 increase is inhibited by naloxone and CTAP. * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Expressing, Staining, Marker, Double Staining

    Up-regulation of MMP-9 expression in DRGs but not spinal cords after intrathecal morphine or remifentanil and potentiation of intrathecal morphine analgesia in Mmp9 deficient mice . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) 2 h after intrathecal injection of morphine (10 nmol) or remifentanil (1 nmol). B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: Up-regulation of MMP-9 expression in DRGs but not spinal cords after intrathecal morphine or remifentanil and potentiation of intrathecal morphine analgesia in Mmp9 deficient mice . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) 2 h after intrathecal injection of morphine (10 nmol) or remifentanil (1 nmol). B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Expressing, Mouse Assay, Western Blot, Injection

    Intrathecal administration of MMP-9 siRNA reduces MMP-9 expression in DRGs and enhances morphine analgesia . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) after intrathecal injections of MMP-9 siRNA or control siRNA (3 μg, 24 and 2 h before the s.c. morphine injection. B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: Intrathecal administration of MMP-9 siRNA reduces MMP-9 expression in DRGs and enhances morphine analgesia . (A-D) Western blotting showing MMP-9 expression in DRGs (A, B) and spinal cord dorsal horns (C, D) after intrathecal injections of MMP-9 siRNA or control siRNA (3 μg, 24 and 2 h before the s.c. morphine injection. B and D are quantification of the MMP-9 bands in DRGs (B) and spinal cords (D). * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Expressing, Western Blot, Injection

    MMP-9 deficiency does not alter opioid-induced hyperalgesia after subcutaneous morphine . (A, B) Western blot gel (A) and band density (B) showing MMP-9 expression in lumbar DRGs 24 h after morphine (MORPH) injection. * P > 0.05, compared to naive control (CTRL), Student's t-test, n = 4 mice. (C, D) Subcutaneous morphine (5 mg/kg) induces comparable heat hyperalgesia in both wild-type and Mmp9 -KO mice (C) and also in wild-type mice pretreated with intrathecal MMP-9 inhibitor-I (0.2 μg) or vehicle (10% DMSO). * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: MMP-9 deficiency does not alter opioid-induced hyperalgesia after subcutaneous morphine . (A, B) Western blot gel (A) and band density (B) showing MMP-9 expression in lumbar DRGs 24 h after morphine (MORPH) injection. * P > 0.05, compared to naive control (CTRL), Student's t-test, n = 4 mice. (C, D) Subcutaneous morphine (5 mg/kg) induces comparable heat hyperalgesia in both wild-type and Mmp9 -KO mice (C) and also in wild-type mice pretreated with intrathecal MMP-9 inhibitor-I (0.2 μg) or vehicle (10% DMSO). * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Western Blot, Expressing, Injection, Mouse Assay

    Subcutaneous morphine induces MMP-9 and TIMP-1 mRNA up-regulation in DRGs . (A, B) RT-PCR showing time course of subcutaneous morphine-induced MMP-9 (A) and TIMP-1 (B) mRNA expression in DRGs. * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: Subcutaneous morphine induces MMP-9 and TIMP-1 mRNA up-regulation in DRGs . (A, B) RT-PCR showing time course of subcutaneous morphine-induced MMP-9 (A) and TIMP-1 (B) mRNA expression in DRGs. * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    Subcutaneous morphine-induced MMP-9 increase is enriched in mu opioid receptor (MOR)-expressing DRG neurons . (A, B) Double staining showing colocalization of MMP-9 and MOR in DRG neurons of saline (A) and morphine (B) treated mice (s.c., 10 mg/kg). Scale, 50 μm. (C, D) Percentage of MMP-9+ (C) and MOR + (D) neurons in DRGs 2 h after saline and morphine injection. (E) Percentage of MMP-9 and MOR double-positive neurons in DRGs 2 h after saline and morphine injection. (F) Percentage of MMP-9-positive neurons expressing MOR and percentage of MOR-positive neurons expressing MMP-9 in DRGs 2 h after saline and morphine injection. S, saline; M, morphine. * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: Subcutaneous morphine-induced MMP-9 increase is enriched in mu opioid receptor (MOR)-expressing DRG neurons . (A, B) Double staining showing colocalization of MMP-9 and MOR in DRG neurons of saline (A) and morphine (B) treated mice (s.c., 10 mg/kg). Scale, 50 μm. (C, D) Percentage of MMP-9+ (C) and MOR + (D) neurons in DRGs 2 h after saline and morphine injection. (E) Percentage of MMP-9 and MOR double-positive neurons in DRGs 2 h after saline and morphine injection. (F) Percentage of MMP-9-positive neurons expressing MOR and percentage of MOR-positive neurons expressing MMP-9 in DRGs 2 h after saline and morphine injection. S, saline; M, morphine. * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Expressing, Double Staining, Mouse Assay, Injection

    Actue morphine treatment does not change the sizes of MMP-9- and MOR-positive neurons in DRGs . (A, B) Size frequency distribution (percentage) of MMP-9-positive neurons (A) and MOR-positive neurons (B) in DRGs 2 h after subcutaneous saline and morphine. 300-500 neurons from 3 animals were plotted in each group. Note that MMP-9 and MOR are mainly expressed in small- and medium-sized neurons.

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: Actue morphine treatment does not change the sizes of MMP-9- and MOR-positive neurons in DRGs . (A, B) Size frequency distribution (percentage) of MMP-9-positive neurons (A) and MOR-positive neurons (B) in DRGs 2 h after subcutaneous saline and morphine. 300-500 neurons from 3 animals were plotted in each group. Note that MMP-9 and MOR are mainly expressed in small- and medium-sized neurons.

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques:

    Subcutaneous morphine increases MMP-9 expression and activity in DRGs . (A) Western blotting showing the time course of MMP-9 expression in lumbar DRGs after morphine (s.c., 10 mg/kg). (B) Gelatin zymography showing the time course of MMP-9 activity in lumbar DRGs after morphine. Note that MMP-2 activity remains unchanged after morphine injection. (C) Western blotting showing the time course of MMP-9 expression in lumbar spinal cord dorsal horns after morphine (s.c., 10 mg/kg). Note that subcutaneous morphine only induces MMP-9 expression in DRGs but not spinal cords. * P

    Journal: Molecular Pain

    Article Title: Acute morphine induces matrix metalloproteinase-9 up-regulation in primary sensory neurons to mask opioid-induced analgesia in mice

    doi: 10.1186/1744-8069-8-19

    Figure Lengend Snippet: Subcutaneous morphine increases MMP-9 expression and activity in DRGs . (A) Western blotting showing the time course of MMP-9 expression in lumbar DRGs after morphine (s.c., 10 mg/kg). (B) Gelatin zymography showing the time course of MMP-9 activity in lumbar DRGs after morphine. Note that MMP-2 activity remains unchanged after morphine injection. (C) Western blotting showing the time course of MMP-9 expression in lumbar spinal cord dorsal horns after morphine (s.c., 10 mg/kg). Note that subcutaneous morphine only induces MMP-9 expression in DRGs but not spinal cords. * P

    Article Snippet: Primary DRG cultures and immunocytochemistry DRGs were removed aseptically from 4-week old mice and first incubated with collagenase (1.25 mg/ml, Roche)/dispase-II (2.4 units/ml, Roche) at 37°C for 90 min, then digested with 0.25% trypsin (Cellgro) for 8 min at 37°C, followed by 0.25% trypsin inhibitor (Sigma).

    Techniques: Expressing, Activity Assay, Western Blot, Zymography, Injection