primary antibodies against nanog  (Millipore)


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    Name:
    Anti NANOG
    Description:

    Catalog Number:
    sab4301837
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    Structured Review

    Millipore primary antibodies against nanog
    Anti NANOG

    https://www.bioz.com/result/primary antibodies against nanog/product/Millipore
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against nanog - by Bioz Stars, 2020-08
    94/100 stars

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    1) Product Images from "Nanog induces suppression of senescence through downregulation of p27KIP1 expression"

    Article Title: Nanog induces suppression of senescence through downregulation of p27KIP1 expression

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.167932

    ChIP analysis reveals Nanog - binding sites regulating p27 KIP1 expression. (A) Schematic representation (not drawn to scale) of the p27 genomic locus (RefSeq: NM_009875) highlighting the location of putative Nanog-binding sites designated ‘D’ (primer pairs designated as D1 and D2) and ‘P’ (primer pairs designated as P1 and P2) in the upstream region of the p27 KIP1 gene ( Chen et al., 2008 ; Marson et al., 2008 ). PCR primer pairs were designed for these sites for ChIP analyses (dumbbell shaped; Table S2 ). (B) ChIP analysis reveals that Nanog protein in ESCs binds within the upstream region of the p27 KIP1 gene. Oct4-GiP MEF and Oct4-GiP ESCs were cultured, harvested and processed for ChIP analysis with beads only, IgG and Nanog antibody. The Oct4-GiP MEF cell line was used as a negative control. Input DNA (10%) was used as a control for ChIP. Beads only and IgG served as negative controls. Putative Nanog-binding regions were amplified by the designed primer pairs (D1, D2, P1 and P2). Primer pairs were also designed randomly in the 3′UTR region of the p27 KIP1 gene to serve as a negative (desert) control (Dc). (C) RT-qPCR analysis on the ChIP samples explained in B using primers pairs ‘P’ (P1) and ‘D’ (D1) to amplify the Nanog-binding p27 KIP1 sites. RT-qPCR was also performed on the Dc primer set but no amplification was observed other than the input samples (data not shown). Two independent biological replicates were performed for ChIP analysis. ** P
    Figure Legend Snippet: ChIP analysis reveals Nanog - binding sites regulating p27 KIP1 expression. (A) Schematic representation (not drawn to scale) of the p27 genomic locus (RefSeq: NM_009875) highlighting the location of putative Nanog-binding sites designated ‘D’ (primer pairs designated as D1 and D2) and ‘P’ (primer pairs designated as P1 and P2) in the upstream region of the p27 KIP1 gene ( Chen et al., 2008 ; Marson et al., 2008 ). PCR primer pairs were designed for these sites for ChIP analyses (dumbbell shaped; Table S2 ). (B) ChIP analysis reveals that Nanog protein in ESCs binds within the upstream region of the p27 KIP1 gene. Oct4-GiP MEF and Oct4-GiP ESCs were cultured, harvested and processed for ChIP analysis with beads only, IgG and Nanog antibody. The Oct4-GiP MEF cell line was used as a negative control. Input DNA (10%) was used as a control for ChIP. Beads only and IgG served as negative controls. Putative Nanog-binding regions were amplified by the designed primer pairs (D1, D2, P1 and P2). Primer pairs were also designed randomly in the 3′UTR region of the p27 KIP1 gene to serve as a negative (desert) control (Dc). (C) RT-qPCR analysis on the ChIP samples explained in B using primers pairs ‘P’ (P1) and ‘D’ (D1) to amplify the Nanog-binding p27 KIP1 sites. RT-qPCR was also performed on the Dc primer set but no amplification was observed other than the input samples (data not shown). Two independent biological replicates were performed for ChIP analysis. ** P

    Techniques Used: Chromatin Immunoprecipitation, Binding Assay, Expressing, Polymerase Chain Reaction, Cell Culture, Negative Control, Amplification, Quantitative RT-PCR

    Related Articles

    Immunoprecipitation:

    Article Title: Nanog induces suppression of senescence through downregulation of p27KIP1 expression
    Article Snippet: .. After dilution, the protein–DNA complexes were immunoprecipitated overnight at 4°C with rotation using primary antibodies against Nanog (AB5731, Millipore) and rabbit control IgG ChIP grade (ab46540, Abcam). .. Immunoprecipitated chromatin was incubated with Protein A/G Plus agarose beads (sc-2003; Santa Cruz Biotechnology) for another 1 h at 4°C and then washed three times with low-salt washing buffer (20 mM Tris-HCl pH 8.0, 1% Triton X-100, 0.1% SDS, 150 mM NaCl, 2 mM EDTA and 0.01% Tween 20) and twice with high-salt washing buffer (20 mM Tris-HCl pH 8.0, 1% Triton X-100, 0.1% SDS, 500 mM NaCl, 2 mM EDTA and 0.01% Tween 20).

    Incubation:

    Article Title: Transposon-mediated BAC transgenesis in human ES cells
    Article Snippet: .. Immunostaining and microscopy The incubation with primary antibodies was for 1 h at room temperature with mouse anti-Oct4 (1:50, sc-5279; Santa Cruz), rabbit anti-Nanog (1:30, AB5731; Chemicon) or overnight at +4°C with mouse anti-Pax6 (1:30; Developmental Studies Hybridoma Bank). .. The cells were incubated with secondary antibodies diluted 1:500 (FITC goat anti-rabbit and TRITC goat anti-mouse; Jackson Immunoresearch Laboratories and Alexa633 goat anti-mouse; Molecular Probes, Invitrogen) for 1 h at room temperature.

    Article Title: Characterization of Bovine Induced Pluripotent Stem Cells by Lentiviral Transduction of Reprogramming Factor Fusion Proteins
    Article Snippet: .. The primary antibodies anti-Oct4 in rabbit (Abcam, ab19875), anti-Nanog in goat (R & D, BAF1997), anti-SSEA1 in mouse (Chemicon, MAB4301), anti-SSEA3 in mouse (Chemicon, MAB4303), anti-SSEA4 in mouse (Chemicon, MAB4304), anti-TRA-1-60 (Chemicon, MAB4360) and TRA-1-80 in mouse (Chemicon, MAB4381), anti-α-Actinin (Sarcomeric) in mouse (Sigma, A7811), Anti-α-Fetoprotein (AFP) in mouse (Sigma, A8452), Anti-Neurofilament 200 in rabbit (Sigma, N4142), anti-Nobox (Abcam, ab41521) in rabbit and anti-Vasa (Abcam, ab13840) in rabbit were diluted 1:200, added onto cells and incubated at 4°C overnight. .. Cells were washed with PBS, incubated with CY3-labeled secondary antibody (1:200 dilution) at room temperature for 1 h and then washed with PBS.

    Chromatin Immunoprecipitation:

    Article Title: Nanog induces suppression of senescence through downregulation of p27KIP1 expression
    Article Snippet: .. After dilution, the protein–DNA complexes were immunoprecipitated overnight at 4°C with rotation using primary antibodies against Nanog (AB5731, Millipore) and rabbit control IgG ChIP grade (ab46540, Abcam). .. Immunoprecipitated chromatin was incubated with Protein A/G Plus agarose beads (sc-2003; Santa Cruz Biotechnology) for another 1 h at 4°C and then washed three times with low-salt washing buffer (20 mM Tris-HCl pH 8.0, 1% Triton X-100, 0.1% SDS, 150 mM NaCl, 2 mM EDTA and 0.01% Tween 20) and twice with high-salt washing buffer (20 mM Tris-HCl pH 8.0, 1% Triton X-100, 0.1% SDS, 500 mM NaCl, 2 mM EDTA and 0.01% Tween 20).

    Immunostaining:

    Article Title: Transposon-mediated BAC transgenesis in human ES cells
    Article Snippet: .. Immunostaining and microscopy The incubation with primary antibodies was for 1 h at room temperature with mouse anti-Oct4 (1:50, sc-5279; Santa Cruz), rabbit anti-Nanog (1:30, AB5731; Chemicon) or overnight at +4°C with mouse anti-Pax6 (1:30; Developmental Studies Hybridoma Bank). .. The cells were incubated with secondary antibodies diluted 1:500 (FITC goat anti-rabbit and TRITC goat anti-mouse; Jackson Immunoresearch Laboratories and Alexa633 goat anti-mouse; Molecular Probes, Invitrogen) for 1 h at room temperature.

    Microscopy:

    Article Title: Transposon-mediated BAC transgenesis in human ES cells
    Article Snippet: .. Immunostaining and microscopy The incubation with primary antibodies was for 1 h at room temperature with mouse anti-Oct4 (1:50, sc-5279; Santa Cruz), rabbit anti-Nanog (1:30, AB5731; Chemicon) or overnight at +4°C with mouse anti-Pax6 (1:30; Developmental Studies Hybridoma Bank). .. The cells were incubated with secondary antibodies diluted 1:500 (FITC goat anti-rabbit and TRITC goat anti-mouse; Jackson Immunoresearch Laboratories and Alexa633 goat anti-mouse; Molecular Probes, Invitrogen) for 1 h at room temperature.

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  • 94
    Millipore primary antibodies against nanog
    ChIP analysis reveals <t>Nanog</t> - binding sites regulating p27 KIP1 expression. (A) Schematic representation (not drawn to scale) of the p27 genomic locus (RefSeq: NM_009875) highlighting the location of putative Nanog-binding sites designated ‘D’ (primer pairs designated as D1 and D2) and ‘P’ (primer pairs designated as P1 and P2) in the upstream region of the p27 KIP1 gene ( Chen et al., 2008 ; Marson et al., 2008 ). PCR primer pairs were designed for these sites for ChIP analyses (dumbbell shaped; Table S2 ). (B) ChIP analysis reveals that Nanog protein in ESCs binds within the upstream region of the p27 KIP1 gene. Oct4-GiP MEF and Oct4-GiP ESCs were cultured, harvested and processed for ChIP analysis with beads only, IgG and Nanog antibody. The Oct4-GiP MEF cell line was used as a negative control. Input <t>DNA</t> (10%) was used as a control for ChIP. Beads only and IgG served as negative controls. Putative Nanog-binding regions were amplified by the designed primer pairs (D1, D2, P1 and P2). Primer pairs were also designed randomly in the 3′UTR region of the p27 KIP1 gene to serve as a negative (desert) control (Dc). (C) RT-qPCR analysis on the ChIP samples explained in B using primers pairs ‘P’ (P1) and ‘D’ (D1) to amplify the Nanog-binding p27 KIP1 sites. RT-qPCR was also performed on the Dc primer set but no amplification was observed other than the input samples (data not shown). Two independent biological replicates were performed for ChIP analysis. ** P
    Primary Antibodies Against Nanog, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against nanog/product/Millipore
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against nanog - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    Image Search Results


    ChIP analysis reveals Nanog - binding sites regulating p27 KIP1 expression. (A) Schematic representation (not drawn to scale) of the p27 genomic locus (RefSeq: NM_009875) highlighting the location of putative Nanog-binding sites designated ‘D’ (primer pairs designated as D1 and D2) and ‘P’ (primer pairs designated as P1 and P2) in the upstream region of the p27 KIP1 gene ( Chen et al., 2008 ; Marson et al., 2008 ). PCR primer pairs were designed for these sites for ChIP analyses (dumbbell shaped; Table S2 ). (B) ChIP analysis reveals that Nanog protein in ESCs binds within the upstream region of the p27 KIP1 gene. Oct4-GiP MEF and Oct4-GiP ESCs were cultured, harvested and processed for ChIP analysis with beads only, IgG and Nanog antibody. The Oct4-GiP MEF cell line was used as a negative control. Input DNA (10%) was used as a control for ChIP. Beads only and IgG served as negative controls. Putative Nanog-binding regions were amplified by the designed primer pairs (D1, D2, P1 and P2). Primer pairs were also designed randomly in the 3′UTR region of the p27 KIP1 gene to serve as a negative (desert) control (Dc). (C) RT-qPCR analysis on the ChIP samples explained in B using primers pairs ‘P’ (P1) and ‘D’ (D1) to amplify the Nanog-binding p27 KIP1 sites. RT-qPCR was also performed on the Dc primer set but no amplification was observed other than the input samples (data not shown). Two independent biological replicates were performed for ChIP analysis. ** P

    Journal: Journal of Cell Science

    Article Title: Nanog induces suppression of senescence through downregulation of p27KIP1 expression

    doi: 10.1242/jcs.167932

    Figure Lengend Snippet: ChIP analysis reveals Nanog - binding sites regulating p27 KIP1 expression. (A) Schematic representation (not drawn to scale) of the p27 genomic locus (RefSeq: NM_009875) highlighting the location of putative Nanog-binding sites designated ‘D’ (primer pairs designated as D1 and D2) and ‘P’ (primer pairs designated as P1 and P2) in the upstream region of the p27 KIP1 gene ( Chen et al., 2008 ; Marson et al., 2008 ). PCR primer pairs were designed for these sites for ChIP analyses (dumbbell shaped; Table S2 ). (B) ChIP analysis reveals that Nanog protein in ESCs binds within the upstream region of the p27 KIP1 gene. Oct4-GiP MEF and Oct4-GiP ESCs were cultured, harvested and processed for ChIP analysis with beads only, IgG and Nanog antibody. The Oct4-GiP MEF cell line was used as a negative control. Input DNA (10%) was used as a control for ChIP. Beads only and IgG served as negative controls. Putative Nanog-binding regions were amplified by the designed primer pairs (D1, D2, P1 and P2). Primer pairs were also designed randomly in the 3′UTR region of the p27 KIP1 gene to serve as a negative (desert) control (Dc). (C) RT-qPCR analysis on the ChIP samples explained in B using primers pairs ‘P’ (P1) and ‘D’ (D1) to amplify the Nanog-binding p27 KIP1 sites. RT-qPCR was also performed on the Dc primer set but no amplification was observed other than the input samples (data not shown). Two independent biological replicates were performed for ChIP analysis. ** P

    Article Snippet: After dilution, the protein–DNA complexes were immunoprecipitated overnight at 4°C with rotation using primary antibodies against Nanog (AB5731, Millipore) and rabbit control IgG ChIP grade (ab46540, Abcam).

    Techniques: Chromatin Immunoprecipitation, Binding Assay, Expressing, Polymerase Chain Reaction, Cell Culture, Negative Control, Amplification, Quantitative RT-PCR