prepared filipin  (Millipore)


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    Structured Review

    Millipore prepared filipin
    Prepared Filipin, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prepared filipin/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    prepared filipin - by Bioz Stars, 2020-04
    91/100 stars

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    Related Articles

    Fluorescence:

    Article Title: GP73 regulates Hepatic Steatosis by enhancing SCAP-SREBPs interaction
    Article Snippet: Paragraph title: Fluorescence imaging and Filipin staining ... For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma) solution (50 μg/ml).

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). .. For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL).

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: Filipin staining For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). .. Filipin staining For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL).

    Microscopy:

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). .. For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL).

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: Filipin staining For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). .. Filipin staining For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL).

    Article Title: Modeling Smith-Lemli-Opitz syndrome with iPS cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes
    Article Snippet: Chambers were fixed with 4% PFA for 30 min, PBS rinsed, permeabilized with 0.2% saponin (Sigma) in 10% goat serum, PBS rinsed, and incubated overnight at 4 °C with 50 μg/mL freshly prepared filipin (Sigma) and rabbit anti-OCT4A diluted in 0.05% saponin/10% goat serum. .. Slides were mounted, imaged with a Leica DM6000B microscope, and processed with Volocity (PerkinElmer) and Adobe Photoshop (Adobe Systems) software.

    Incubation:

    Article Title: GP73 regulates Hepatic Steatosis by enhancing SCAP-SREBPs interaction
    Article Snippet: .. For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma) solution (50 μg/ml). ..

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: .. For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). ..

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: .. Filipin staining For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). ..

    Article Title: Modeling Smith-Lemli-Opitz syndrome with iPS cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes
    Article Snippet: .. Chambers were fixed with 4% PFA for 30 min, PBS rinsed, permeabilized with 0.2% saponin (Sigma) in 10% goat serum, PBS rinsed, and incubated overnight at 4 °C with 50 μg/mL freshly prepared filipin (Sigma) and rabbit anti-OCT4A diluted in 0.05% saponin/10% goat serum. ..

    Imaging:

    Article Title: GP73 regulates Hepatic Steatosis by enhancing SCAP-SREBPs interaction
    Article Snippet: Paragraph title: Fluorescence imaging and Filipin staining ... For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma) solution (50 μg/ml).

    Staining:

    Article Title: GP73 regulates Hepatic Steatosis by enhancing SCAP-SREBPs interaction
    Article Snippet: .. For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma) solution (50 μg/ml). ..

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: .. For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). ..

    Article Title: Sauchinone controls hepatic cholesterol homeostasis by the negative regulation of PCSK9 transcriptional network
    Article Snippet: .. Filipin staining For Filipin staining, cells grown on coverslips were fixed with 4% paraformaldehyde for 30 min at room temperature, followed by 2 hrs incubation in a freshly prepared Filipin III (Sigma-Aldrich, St. Louis, MO, USA) solution (50 μg/mL). ..

    Article Title: Modeling Smith-Lemli-Opitz syndrome with iPS cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes
    Article Snippet: Paragraph title: Filipin staining of NPC iPS cells ... Chambers were fixed with 4% PFA for 30 min, PBS rinsed, permeabilized with 0.2% saponin (Sigma) in 10% goat serum, PBS rinsed, and incubated overnight at 4 °C with 50 μg/mL freshly prepared filipin (Sigma) and rabbit anti-OCT4A diluted in 0.05% saponin/10% goat serum.

    Software:

    Article Title: Modeling Smith-Lemli-Opitz syndrome with iPS cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes
    Article Snippet: Chambers were fixed with 4% PFA for 30 min, PBS rinsed, permeabilized with 0.2% saponin (Sigma) in 10% goat serum, PBS rinsed, and incubated overnight at 4 °C with 50 μg/mL freshly prepared filipin (Sigma) and rabbit anti-OCT4A diluted in 0.05% saponin/10% goat serum. .. Slides were mounted, imaged with a Leica DM6000B microscope, and processed with Volocity (PerkinElmer) and Adobe Photoshop (Adobe Systems) software.

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  • 99
    Millipore filipin iii
    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL <t>filipin</t> <t>III</t> for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p
    Filipin Iii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/filipin iii/product/Millipore
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    filipin iii - by Bioz Stars, 2020-04
    99/100 stars
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    Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL filipin III for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p

    Journal: Laboratory investigation; a journal of technical methods and pathology

    Article Title: Anandamide exerts its antiproliferative actions on cholangiocarcinoma by activation of the GPR55 receptor

    doi: 10.1038/labinvest.2011.62

    Figure Lengend Snippet: Disruption of lipid rafts inhibits the antiproliferative effects of GPR55 activation. ( A ) Mz-ChA-1 cells were pretreated with lipid raft disrupters 0.1 mM β–methylcyclodextrin, or 1 μg/mL filipin III for 1 hr prior to the addition of O-1602 (10 −5 M). Cell viability was determined by MTS assays. Data are expressed as average ± SEM (*p

    Article Snippet: The inhibitors utilized were the lipid raft disruptors, methyl-β-cyclodextrin (0.1 mM), , and filipin III (1 mg/mL) ( , ), and the JNK inhibitor (10−7 M; SP600125; EMD Chemicals, Gibbstown, NJ) ( ).

    Techniques: Activation Assay