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Phenomenex preparative reversed phase hplc
Preparative Reversed Phase Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 91/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preparative reversed phase hplc/product/Phenomenex
Average 91 stars, based on 23 article reviews
Price from $9.99 to $1999.99
preparative reversed phase hplc - by Bioz Stars, 2020-11
91/100 stars

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High Performance Liquid Chromatography:

Article Title: Amino Acid Sequence in Constitutionally Isomeric Tetrapeptide Amphiphiles Dictates Architecture of One-Dimensional Nanostructures
Article Snippet: .. The peptides were purified by preparative RP-HPLC using a Phenomenex Gemini column (C18, 10 μm, 100 Å, 30 mm × 150 mm) at 25 °C on a Varian Prostar Model 210 preparative HPLC system. .. A water/acetonitrile gradient containing 0.1% v/v NH4 OH was used as an eluent at a flow rate of 25 mL/min.

Article Title: Cyclooxygenase Allosterism, Fatty Acid-mediated Cross-talk between Monomers of Cyclooxygenase Homodimers
Article Snippet: .. Preparative reversed-phase HPLC was used to separate the fatty acid methyl esters (7.34 g) present in the resulting colorless oil mixture of fatty acid methyl esters; the HPLC conditions were as follows: column Phenomenex Luna-C18 (2) (22 × 250 mm), 5 μm with a mobile phase of acetonitrile-water-acetic acid (65:35:0.1, v/v) and a flow rate of 30 ml/min. ..

Article Title: Cysteine-Rich Peptide Fingerprinting as a General Method for Herbal Analysis to Differentiate Radix Astragali and Radix Hedysarum
Article Snippet: .. Fractions with the desired peptides were pooled and purified by multiple runs of preparative RP-HPLC using a C18 column (particle size, 5 μm, 250 × 21 mm; Phenomenex, CA, United States) on a Shimadzu HPLC system (Shimadzu, Kyoto, Japan). ..

Article Title: Macrophilones from the Marine Hydroid Macrorhynchia philippina Can Inhibit ERK Cascade Signaling
Article Snippet: .. Preparative reversed-phase HPLC was run on a Varian PrepStar preparative HPLC system using a Phenomenex Jupiter C18 (5 μ m, 300 Å, 250 × 10 mm) column run with the indicated gradient. (+)HRESIMS data were acquired on an Agilent Technology 6530 Accurate-mass Q-TOF LC/MS. .. Specimens of the hydroid Macrorhynchia philippina were collected in Northwestern Australia in August 1988, under contract through the Coral Reef Research Foundation for the Natural Products Branch, National Center Institute.

Flow Cytometry:

Article Title: Cyclooxygenase Allosterism, Fatty Acid-mediated Cross-talk between Monomers of Cyclooxygenase Homodimers
Article Snippet: .. Preparative reversed-phase HPLC was used to separate the fatty acid methyl esters (7.34 g) present in the resulting colorless oil mixture of fatty acid methyl esters; the HPLC conditions were as follows: column Phenomenex Luna-C18 (2) (22 × 250 mm), 5 μm with a mobile phase of acetonitrile-water-acetic acid (65:35:0.1, v/v) and a flow rate of 30 ml/min. ..

Article Title: Cytotoxicity of Endoperoxides from the Caribbean Sponge Plakortis halichondrioides towards Sensitive and Multidrug-Resistant Leukemia Cells: Acids vs. Esters Activity Evaluation
Article Snippet: .. The raw product was further purified via preparative RP-HPLC (Phenomenex Hyperclone 5 µ) using methanol/acetonitrile/water 85:6:9 containing 0.1% formic acid (flow 9 mL/min). .. Plakortide E methyl ester eluted at 14 min. Colourless viscous oil (3.2 mg, 33%); [ α ] D 23 = +74.1 (c 0.00305, CHCl3 ); LC-MS: m /z 403.9 [M + K]+ , calcd. for C22 H36 O4 m /z 364.52.

Liquid Chromatography with Mass Spectroscopy:

Article Title: Macrophilones from the Marine Hydroid Macrorhynchia philippina Can Inhibit ERK Cascade Signaling
Article Snippet: .. Preparative reversed-phase HPLC was run on a Varian PrepStar preparative HPLC system using a Phenomenex Jupiter C18 (5 μ m, 300 Å, 250 × 10 mm) column run with the indicated gradient. (+)HRESIMS data were acquired on an Agilent Technology 6530 Accurate-mass Q-TOF LC/MS. .. Specimens of the hydroid Macrorhynchia philippina were collected in Northwestern Australia in August 1988, under contract through the Coral Reef Research Foundation for the Natural Products Branch, National Center Institute.

Purification:

Article Title: REG-O3 chimeric peptide combining growth hormone and somatostatin sequences improves joint function and prevents cartilage degradation in rat model of traumatic knee osteoarthritis
Article Snippet: .. The crude peptide was purified using a preparative RP-HPLC ( R everse P hase- H igh P erformance L iquid C hromatography) system, with a C18 column from Phenomenex (100 Å 15 μm, 250*50 mm–reference 00G-4273-V0-AX) working at 93 mL/min, a solvent system containing Buffer A: 0.1% TFA/water—Buffer B: 0.1% TFA /water/acetonitrile and a gradient of 25 to 53% of buffer B in 24 min. .. The semi pure peptide obtained above was finally purified (5 g per injection) using an ion exchange RP-HPLC system with a C18 column from Phenomenex (100 Å 15 μm, 250*50 mm–reference 00G-4273-V0-AX) working at 93 mL/min using as solvent system Buffer A: 0.1% Ac-OH/water—Buffer B: 0.1% Ac-OH/water/acetonitrile and a gradient of 0 to 100% of buffer B in 25 min.

Article Title: Amino Acid Sequence in Constitutionally Isomeric Tetrapeptide Amphiphiles Dictates Architecture of One-Dimensional Nanostructures
Article Snippet: .. The peptides were purified by preparative RP-HPLC using a Phenomenex Gemini column (C18, 10 μm, 100 Å, 30 mm × 150 mm) at 25 °C on a Varian Prostar Model 210 preparative HPLC system. .. A water/acetonitrile gradient containing 0.1% v/v NH4 OH was used as an eluent at a flow rate of 25 mL/min.

Article Title: Cysteine-Rich Peptide Fingerprinting as a General Method for Herbal Analysis to Differentiate Radix Astragali and Radix Hedysarum
Article Snippet: .. Fractions with the desired peptides were pooled and purified by multiple runs of preparative RP-HPLC using a C18 column (particle size, 5 μm, 250 × 21 mm; Phenomenex, CA, United States) on a Shimadzu HPLC system (Shimadzu, Kyoto, Japan). ..

Article Title: Cytotoxicity of Endoperoxides from the Caribbean Sponge Plakortis halichondrioides towards Sensitive and Multidrug-Resistant Leukemia Cells: Acids vs. Esters Activity Evaluation
Article Snippet: .. The raw product was further purified via preparative RP-HPLC (Phenomenex Hyperclone 5 µ) using methanol/acetonitrile/water 85:6:9 containing 0.1% formic acid (flow 9 mL/min). .. Plakortide E methyl ester eluted at 14 min. Colourless viscous oil (3.2 mg, 33%); [ α ] D 23 = +74.1 (c 0.00305, CHCl3 ); LC-MS: m /z 403.9 [M + K]+ , calcd. for C22 H36 O4 m /z 364.52.

Article Title: Identification of a Peptide for Systemic Brain Delivery of a Morpholino Oligonucleotide in Mouse Models of Spinal Muscular Atrophy
Article Snippet: .. All peptides were purified ( > 90%) using preparative RP-HPLC on a Phenomenex column (21.2 × 150 mm, C18, 5 μm) using a 0.1% TFA-based buffer. .. MALDI-TOF mass spectrometry (ABI Voyager DE Pro) was used to characterize the peptides using either α-cyano-4-hydroxycinnamic acid or sinapic acid matrices.

Article Title: NMR Structure of μ-Conotoxin GIIIC: Leucine 18 Induces Local Repacking of the N-Terminus Resulting in Reduced NaV Channel Potency
Article Snippet: .. The target peptide was purified by preparative RP-HPLC on a Phenomenex C18 column using linear gradients of solvent B (ACN:H2 O:TFA; 89.5:10:0.05) in solvent A (H2 O:TFA; 99.5:0.05). .. Peptide purity and identity were assessed by ESI-MS on a API-2000 mass spectrometer (Applied Biosystems, Foster City, CA, USA) and by analytical scale μHPLC on a Nexera system (Shimadzu, Melbourne, VIC, Australia) equipped with an Agilent Zorbax C18 column (1.8 μm, 2.1 × 100 mm).

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  • 90
    Phenomenex semi preparative reversed phase hplc
    Chromatography elution profiles and calcium binding capacities of calcium-binding peptides. ( A ) <t>Sephadex</t> G-25 gel filtration chromatography of SPH; ( B ) Semi-preparative C18 <t>RP-HPLC</t> of fraction III; ( C ) RP-HPLC of fraction 17 from semi-preparative HPLC.
    Semi Preparative Reversed Phase Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 90/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative reversed phase hplc/product/Phenomenex
    Average 90 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    semi preparative reversed phase hplc - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    89
    Phenomenex semi preparative reverse phase hplc
    LC-DAD-MS Analysis of wild-Type A. nidulans and alcA (p)- micA mutant metabolites. ( a ) <t>HPLC</t> profiles of extracts as detected by UV absorption. ( b ) UV-Vis and ESIMS spectra (positive mode) of <t>microperfuranone.</t>
    Semi Preparative Reverse Phase Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparative reverse phase hplc/product/Phenomenex
    Average 89 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    semi preparative reverse phase hplc - by Bioz Stars, 2020-11
    89/100 stars
      Buy from Supplier

    90
    Phenomenex semi preparation reversed phase hplc
    Elution profiles and calcium-binding capacities of calcium-binding peptides. ( a ) <t>Sephadex</t> G-25 gel filtration chromatography of SPH; ( b ) semi-preparative C18 <t>RP-HPLC</t> of fraction F3; and ( c ) analytic RP-HPLC of fraction 17 from semi-preparative HPLC.
    Semi Preparation Reversed Phase Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/semi preparation reversed phase hplc/product/Phenomenex
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    semi preparation reversed phase hplc - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    90
    Phenomenex α elapitoxin ot1a
    Effect of <t>α-elapitoxin-Ot1a</t> (0.1 µM or 1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) on contractile responses of the chick biventer cervicis nerve-muscle preparation to acetylcholine (ACh; 1 mM), carbachol (CCh; 20 µM) and potassium chloride (KCl; 40 mM).
    α Elapitoxin Ot1a, supplied by Phenomenex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/α elapitoxin ot1a/product/Phenomenex
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    α elapitoxin ot1a - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

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    Chromatography elution profiles and calcium binding capacities of calcium-binding peptides. ( A ) Sephadex G-25 gel filtration chromatography of SPH; ( B ) Semi-preparative C18 RP-HPLC of fraction III; ( C ) RP-HPLC of fraction 17 from semi-preparative HPLC.

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: A Specific Peptide with Calcium-Binding Capacity from Defatted Schizochytrium sp. Protein Hydrolysates and the Molecular Properties

    doi: 10.3390/molecules22040544

    Figure Lengend Snippet: Chromatography elution profiles and calcium binding capacities of calcium-binding peptides. ( A ) Sephadex G-25 gel filtration chromatography of SPH; ( B ) Semi-preparative C18 RP-HPLC of fraction III; ( C ) RP-HPLC of fraction 17 from semi-preparative HPLC.

    Article Snippet: The fraction with the highest calcium-binding activity from Sephadex G-25 chromatography was pooled and further purified by semi-preparative reversed phase HPLC on a C18 reversed-silica gel column (Gemini 5 μ C18, 250 × 10 mm; Phenomenex Inc., Torrance, CA, USA).

    Techniques: Chromatography, Binding Assay, Filtration, High Performance Liquid Chromatography

    LC-DAD-MS Analysis of wild-Type A. nidulans and alcA (p)- micA mutant metabolites. ( a ) HPLC profiles of extracts as detected by UV absorption. ( b ) UV-Vis and ESIMS spectra (positive mode) of microperfuranone.

    Journal: Applied microbiology and biotechnology

    Article Title: Molecular genetic analysis reveals that a nonribosomal peptide synthetase-like (NRPS-like) gene in Aspergillus nidulans is responsible for microperfuranone biosynthesis

    doi: 10.1007/s00253-012-4098-9

    Figure Lengend Snippet: LC-DAD-MS Analysis of wild-Type A. nidulans and alcA (p)- micA mutant metabolites. ( a ) HPLC profiles of extracts as detected by UV absorption. ( b ) UV-Vis and ESIMS spectra (positive mode) of microperfuranone.

    Article Snippet: Fraction C containing microperfuranone was further subjected to semi-preparative reverse phase HPLC (Phenomenex Luna 5 μm C18 , 250 × 21.2 mm) with a flow rate of 5.0 ml/min and measured by a UV detector at 254 nm.

    Techniques: Mass Spectrometry, Mutagenesis, High Performance Liquid Chromatography

    Elution profiles and calcium-binding capacities of calcium-binding peptides. ( a ) Sephadex G-25 gel filtration chromatography of SPH; ( b ) semi-preparative C18 RP-HPLC of fraction F3; and ( c ) analytic RP-HPLC of fraction 17 from semi-preparative HPLC.

    Journal: Marine Drugs

    Article Title: Novel Peptide with Specific Calcium-Binding Capacity from Schizochytrium sp. Protein Hydrolysates and Calcium Bioavailability in Caco-2 Cells

    doi: 10.3390/md15010003

    Figure Lengend Snippet: Elution profiles and calcium-binding capacities of calcium-binding peptides. ( a ) Sephadex G-25 gel filtration chromatography of SPH; ( b ) semi-preparative C18 RP-HPLC of fraction F3; and ( c ) analytic RP-HPLC of fraction 17 from semi-preparative HPLC.

    Article Snippet: The fraction with the highest calcium-binding activity from Sephadex G-25 chromatography was pooled and further purified by semi-preparation reversed phase HPLC on a C18 reversed-silica gel chromatograph (Gemini 5 μ C18, 250 × 10 mm; Phenomenex Inc.; Torrance, CA, USA).

    Techniques: Binding Assay, Filtration, Chromatography, High Performance Liquid Chromatography

    Effect of α-elapitoxin-Ot1a (0.1 µM or 1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) on contractile responses of the chick biventer cervicis nerve-muscle preparation to acetylcholine (ACh; 1 mM), carbachol (CCh; 20 µM) and potassium chloride (KCl; 40 mM).

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: Effect of α-elapitoxin-Ot1a (0.1 µM or 1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) on contractile responses of the chick biventer cervicis nerve-muscle preparation to acetylcholine (ACh; 1 mM), carbachol (CCh; 20 µM) and potassium chloride (KCl; 40 mM).

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques:

    Effect of increasing concentrations of α-elapitoxin-Ot1a (10–30 nM; n = 3–4) on cumulative concentration-response curves to carbachol (CCh) in the chick biventer cervicis nerve-muscle preparation. * p

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: Effect of increasing concentrations of α-elapitoxin-Ot1a (10–30 nM; n = 3–4) on cumulative concentration-response curves to carbachol (CCh) in the chick biventer cervicis nerve-muscle preparation. * p

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques: Concentration Assay

    RP-HPLC chromatograph of ( a ) O. temporalis venom run on a Jupiter semi preparative C18 column and ( b ) α-elapitoxin-Ot1a run on a Jupiter analytical C18 column.

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: RP-HPLC chromatograph of ( a ) O. temporalis venom run on a Jupiter semi preparative C18 column and ( b ) α-elapitoxin-Ot1a run on a Jupiter analytical C18 column.

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques: High Performance Liquid Chromatography

    Effect of ( a ) α-elapitoxin-Ot1a (0.1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) or ( b ) α-elapitoxin-Ot1a (1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) on indirect twitches of the chick biventer cervicis nerve-muscle preparation. * p

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: Effect of ( a ) α-elapitoxin-Ot1a (0.1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) or ( b ) α-elapitoxin-Ot1a (1 µM) alone and in the presence of taipan antivenom (AV; 10 U/mL) on indirect twitches of the chick biventer cervicis nerve-muscle preparation. * p

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques:

    Sequence alignment (from BLAST search) of α-elapitoxin-Ot1a with short-chain postsynaptic neurotoxins from Oxyuranus spp. Shaded amino acids are similar to α-elapitoxin-Ot1a. Amino acids with (*) are fully conserved in all toxins, conserved amino acids with (.) are weakly similar properties group and amino acids with (:) are strongly similar properties group.

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: Sequence alignment (from BLAST search) of α-elapitoxin-Ot1a with short-chain postsynaptic neurotoxins from Oxyuranus spp. Shaded amino acids are similar to α-elapitoxin-Ot1a. Amino acids with (*) are fully conserved in all toxins, conserved amino acids with (.) are weakly similar properties group and amino acids with (:) are strongly similar properties group.

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques: Sequencing

    MALDI-TOF of α-elapitoxin-Ot1a indicating a molecular weight of 6712 Da. Proteins were analysed in Linear mode with a mass range of 5 kDa to 120 kDa.

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: MALDI-TOF of α-elapitoxin-Ot1a indicating a molecular weight of 6712 Da. Proteins were analysed in Linear mode with a mass range of 5 kDa to 120 kDa.

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques: Molecular Weight

    Structure of α-elapitoxin Ot1a based on the structure of erabutoxin B [ 24 ].

    Journal: Toxins

    Article Title: Isolation and Pharmacological Characterization of α-Elapitoxin-Ot1a, a Short-Chain Postsynaptic Neurotoxin from the Venom of the Western Desert Taipan, Oxyuranus temporalis

    doi: 10.3390/toxins8030058

    Figure Lengend Snippet: Structure of α-elapitoxin Ot1a based on the structure of erabutoxin B [ 24 ].

    Article Snippet: Individual peaks were collected manually, frozen at −80 °C and then subsequently freeze-dried. α-Elapitoxin-Ot1a (i.e. , the peak at 15 min from the semi preparative reverse-phase HPLC) was further purified on a Phenomenex Jupiter analytical (150 mm × 2 mm, 5 µm, 300 Å) C18 after equilibrating with solvent A (0.1% TFA).

    Techniques: