preparative hsccc separation  (Agilent technologies)

 
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    Agilent technologies preparative hsccc separation
    (a) <t>HPLC</t> chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with <t>HSCCC.</t> Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.
    Preparative Hsccc Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative hsccc separation/product/Agilent technologies
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    preparative hsccc separation - by Bioz Stars, 2020-08
    92/100 stars

    Related Products / Commonly Used Together

    agilent 1100/msg1946
    dpph-hplc assay
    electrospray ionization mass spectrometry esi-ms
    hplc

    Images

    1) Product Images from "Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities"

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

    Journal: Journal of Analytical Methods in Chemistry

    doi: 10.1155/2018/3158293

    (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.
    Figure Legend Snippet: (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Techniques Used: High Performance Liquid Chromatography, Purification, High Speed Counter-current Chromatography, Flow Cytometry, Injection

    2) Product Images from "Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities"

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

    Journal: Journal of Analytical Methods in Chemistry

    doi: 10.1155/2018/3158293

    (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.
    Figure Legend Snippet: (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Techniques Used: High Performance Liquid Chromatography, Purification, High Speed Counter-current Chromatography, Flow Cytometry, Injection

    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. Analysis and Identification of the Target Compound The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. Antioxidant Capacity Assay by Luminol Chemiluminescence The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    Nuclear Magnetic Resonance:

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. Analysis and Identification of the Target Compound The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. Antioxidant Capacity Assay by Luminol Chemiluminescence The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    High Speed Counter-current Chromatography:

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. Analysis and Identification of the Target Compound The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. Antioxidant Capacity Assay by Luminol Chemiluminescence The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    Mass Spectrometry:

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. Analysis and Identification of the Target Compound The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. Antioxidant Capacity Assay by Luminol Chemiluminescence The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities
    Article Snippet: .. The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA). .. The antioxidant activity of the target compound was evaluated by measuring the scavenging percentage of superoxide anion, hydrogen peroxide, and hydroxyl radical with luminol chemiluminescence according to the previous reference [ ].

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    • preparative hsccc separation  (Agilent technologies)
    92
    Agilent technologies preparative hsccc separation
    (a) <t>HPLC</t> chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with <t>HSCCC.</t> Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.
    Preparative Hsccc Separation, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative hsccc separation/product/Agilent technologies
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    preparative hsccc separation - by Bioz Stars, 2020-08
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

    doi: 10.1155/2018/3158293

    Figure Lengend Snippet: (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Article Snippet: The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA).

    Techniques: High Performance Liquid Chromatography, Purification, High Speed Counter-current Chromatography, Flow Cytometry, Injection

    (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Journal: Journal of Analytical Methods in Chemistry

    Article Title: Rapid Screening and Preparative Isolation of Antioxidants from Alpinia officinarum Hance Using HSCCC Coupled with DPPH-HPLC Assay and Evaluation of Their Antioxidant Activities

    doi: 10.1155/2018/3158293

    Figure Lengend Snippet: (a) HPLC chromatogram of the ethyl acetate extract from A. officinarum Hance; (b, c) HPLC analyses of target-separated antioxidants purified with HSCCC. Experimental conditions: column, Shim-pack VP-ODS column (250 × 4.6 mm I.D., 5 μm); column temperature, 25°C; mobile phase, 0.2% acetic acid-acetonitrile with the gradient (0–5 min, 55–68% B; 5–15 min, 68% B; 15–30 min, 68–75% B); flow rate, 1.0 mL/min; detection, 254 nm; injection volume, 20 μL.

    Article Snippet: Analysis and Identification of the Target Compound The target compound from the preparative HSCCC separation was analyzed by HPLC using the chromatographic separations as described in DPPH-HPLC assay and identified by electrospray ionization mass spectrometry (ESI-MS) on an Agilent 1100/MSG1946 (Agilent, California, USA) and 1 H and 13 C NMR spectra on a Varian-600 NMR spectrometer (Varian, Palo Alto, USA).

    Techniques: High Performance Liquid Chromatography, Purification, High Speed Counter-current Chromatography, Flow Cytometry, Injection