Structured Review

Waters Corporation preparative hplc
<t>Preparative-HPLC</t> chromatograms of six ethyl-linked anthocyanin-flavanol pigments. ( A ) 1: <t>malvidin-3-glucoside-ethyl-epicatechin</t> ( S ); 2: malvidin-3-glucoside-ethyl-epicatechin ( R ). ( B ) 1: cyanidin-3-glucoside-ethyl-epicatechin ( S ); 2: cyanidin-3-glucoside-ethyl-epicatechin ( R ). ( C ) 1: peonidin-3-glucoside-ethyl-epicatechin ( S ); 2: peonidin-3-glucoside-ethyl-epicatechin ( R ).
Preparative Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 168 article reviews
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preparative hplc - by Bioz Stars, 2020-11
94/100 stars

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1) Product Images from "Preparation and Antioxidant Activity of Ethyl-Linked Anthocyanin-Flavanol Pigments from Model Wine Solutions"

Article Title: Preparation and Antioxidant Activity of Ethyl-Linked Anthocyanin-Flavanol Pigments from Model Wine Solutions

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules23051066

Preparative-HPLC chromatograms of six ethyl-linked anthocyanin-flavanol pigments. ( A ) 1: malvidin-3-glucoside-ethyl-epicatechin ( S ); 2: malvidin-3-glucoside-ethyl-epicatechin ( R ). ( B ) 1: cyanidin-3-glucoside-ethyl-epicatechin ( S ); 2: cyanidin-3-glucoside-ethyl-epicatechin ( R ). ( C ) 1: peonidin-3-glucoside-ethyl-epicatechin ( S ); 2: peonidin-3-glucoside-ethyl-epicatechin ( R ).
Figure Legend Snippet: Preparative-HPLC chromatograms of six ethyl-linked anthocyanin-flavanol pigments. ( A ) 1: malvidin-3-glucoside-ethyl-epicatechin ( S ); 2: malvidin-3-glucoside-ethyl-epicatechin ( R ). ( B ) 1: cyanidin-3-glucoside-ethyl-epicatechin ( S ); 2: cyanidin-3-glucoside-ethyl-epicatechin ( R ). ( C ) 1: peonidin-3-glucoside-ethyl-epicatechin ( S ); 2: peonidin-3-glucoside-ethyl-epicatechin ( R ).

Techniques Used: High Performance Liquid Chromatography

Chromatograms of HPLC analysis of the reaction between cyanidin-3-glucoside and (–)-epicatechin at 0 h ( a ), 2 h ( b ), 224 h ( c ), 336 h ( d ); Chromatograms of HPLC analysis of the reaction between malvidin-3-glucoside and (–)-epicatechin at 0h ( e ), 2 h ( f ), 224 h ( g ), 336 h ( h ); Chromatograms of HPLC analysis of the reaction between peonidin-3-glucoside and (–)-epicatechin at 0 h ( i ), 2 h ( j ), 224 h ( k ), 336 h ( l ).
Figure Legend Snippet: Chromatograms of HPLC analysis of the reaction between cyanidin-3-glucoside and (–)-epicatechin at 0 h ( a ), 2 h ( b ), 224 h ( c ), 336 h ( d ); Chromatograms of HPLC analysis of the reaction between malvidin-3-glucoside and (–)-epicatechin at 0h ( e ), 2 h ( f ), 224 h ( g ), 336 h ( h ); Chromatograms of HPLC analysis of the reaction between peonidin-3-glucoside and (–)-epicatechin at 0 h ( i ), 2 h ( j ), 224 h ( k ), 336 h ( l ).

Techniques Used: High Performance Liquid Chromatography

2) Product Images from "Benzanthric Acid, a Novel Metabolite From Streptomyces albus Del14 Expressing the Nybomycin Gene Cluster"

Article Title: Benzanthric Acid, a Novel Metabolite From Streptomyces albus Del14 Expressing the Nybomycin Gene Cluster

Journal: Frontiers in Chemistry

doi: 10.3389/fchem.2019.00896

HPLC-MS analysis of benzanthric acid production. (A) Base peak chromatograms of crude extracts from the parent strain S. albus subsp. chlorinus , the recombinant strain S. albus 4N24 harboring the nybomycin gene cluster and the host strain S. albus Del14. Peaks corresponding to benzanthric acid and nybomycin are indicated by 1 and 2 , respectively. (B) UV spectrum of benzanthric acid. (C) Mass spectrum of benzanthric acid ( m/z 256.0 [M+H] + ). The signal at m/z 238.0 corresponds to the [M+H-H 2 O] + ion and the signal at m/z 511.1 corresponds to the [2M+H] + ion.
Figure Legend Snippet: HPLC-MS analysis of benzanthric acid production. (A) Base peak chromatograms of crude extracts from the parent strain S. albus subsp. chlorinus , the recombinant strain S. albus 4N24 harboring the nybomycin gene cluster and the host strain S. albus Del14. Peaks corresponding to benzanthric acid and nybomycin are indicated by 1 and 2 , respectively. (B) UV spectrum of benzanthric acid. (C) Mass spectrum of benzanthric acid ( m/z 256.0 [M+H] + ). The signal at m/z 238.0 corresponds to the [M+H-H 2 O] + ion and the signal at m/z 511.1 corresponds to the [2M+H] + ion.

Techniques Used: High Performance Liquid Chromatography, Recombinant

3) Product Images from "β-Mannanase-catalyzed synthesis of alkyl mannooligosides"

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides

Journal: Applied Microbiology and Biotechnology

doi: 10.1007/s00253-018-8997-2

Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)
Figure Legend Snippet: Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

4) Product Images from "Uncovering production of specialized metabolites by Streptomyces argillaceus: Activation of cryptic biosynthesis gene clusters using nutritional and genetic approaches"

Article Title: Uncovering production of specialized metabolites by Streptomyces argillaceus: Activation of cryptic biosynthesis gene clusters using nutritional and genetic approaches

Journal: PLoS ONE

doi: 10.1371/journal.pone.0198145

Production of specialized metabolites by S . argillaceus . (A) antimycins (peaks 1 to 8 ): UPLC chromatogram at 230 nm of S . argillaceus grown in R5A (black) and in SM30 (red) media; (B) carotenoids: (left) Erlenmeyer flasks containing cultures of S . albus -pKC505 and S . albus -pKC505-C25, and (right) HPLC chromatograms at 450 nm showing production of carotenoids (peaks 9 to 15 ) of S . albus -pKC505 (black) and S . albus -pKC505-C25 (red) in SM17; (C) germicidins (peaks 16 and 17 ): UPLC chromatogram at 290 nm of S . argillaceus Δ adpAa (red) and S . argillaceus wild type (black). M , mithramycin; (D) desferrioxamine B (peak 18 ): HPLC chromatogram at 210 nm of S . argillaceus -pHJLAbrC3c (red) and S . argillaceus -pHJL401c (black). Asterisks correspond to increased production of unknown compounds.
Figure Legend Snippet: Production of specialized metabolites by S . argillaceus . (A) antimycins (peaks 1 to 8 ): UPLC chromatogram at 230 nm of S . argillaceus grown in R5A (black) and in SM30 (red) media; (B) carotenoids: (left) Erlenmeyer flasks containing cultures of S . albus -pKC505 and S . albus -pKC505-C25, and (right) HPLC chromatograms at 450 nm showing production of carotenoids (peaks 9 to 15 ) of S . albus -pKC505 (black) and S . albus -pKC505-C25 (red) in SM17; (C) germicidins (peaks 16 and 17 ): UPLC chromatogram at 290 nm of S . argillaceus Δ adpAa (red) and S . argillaceus wild type (black). M , mithramycin; (D) desferrioxamine B (peak 18 ): HPLC chromatogram at 210 nm of S . argillaceus -pHJLAbrC3c (red) and S . argillaceus -pHJL401c (black). Asterisks correspond to increased production of unknown compounds.

Techniques Used: High Performance Liquid Chromatography

5) Product Images from "Identification of the Potential Biological Preservative Tetramycin A-Producing Strain and Enhancing Its Production"

Article Title: Identification of the Potential Biological Preservative Tetramycin A-Producing Strain and Enhancing Its Production

Journal: Frontiers in Microbiology

doi: 10.3389/fmicb.2019.02925

Purification and determination the structure of the active metabolite. Different fractions obtained by Waters’ preparative HPLC and fraction VII showed significant antifungal activity (A) . Structure of TMA according to NMR (B) .
Figure Legend Snippet: Purification and determination the structure of the active metabolite. Different fractions obtained by Waters’ preparative HPLC and fraction VII showed significant antifungal activity (A) . Structure of TMA according to NMR (B) .

Techniques Used: Purification, High Performance Liquid Chromatography, Activity Assay, Nuclear Magnetic Resonance

6) Product Images from "β-Mannanase-catalyzed synthesis of alkyl mannooligosides"

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides

Journal: Applied Microbiology and Biotechnology

doi: 10.1007/s00253-018-8997-2

Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)
Figure Legend Snippet: Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

7) Product Images from "The Nonantibiotic Small Molecule Cyslabdan Enhances the Potency of ?-Lactams against MRSA by Inhibiting Pentaglycine Interpeptide Bridge Synthesis"

Article Title: The Nonantibiotic Small Molecule Cyslabdan Enhances the Potency of ?-Lactams against MRSA by Inhibiting Pentaglycine Interpeptide Bridge Synthesis

Journal: PLoS ONE

doi: 10.1371/journal.pone.0048981

HPLC analysis of the peptidoglycan composition of cyslabdan-treated MRSA. (A) HPLC analysis of the muropeptides extracted from MRSA peptidoglycans. Peptidoglycans were prepared from MRSA in the presence or absence of cyslabdan (4 µg/mL). After treating the peptidoglycans with mutanolysin and sodium tetrahydroborate, the resultant monomer-, dimer-, trimer-, and oligomer-type muropeptide products were analyzed by HPLC under the established conditions using an ODS column. The upper chromatogram indicates the results obtained for control MRSA, whereas the lower chromatogram indicates those obtained for cyslabdan-treated MRSA. (B) Structures of monomer-type muropeptides identified by ESI-MS. The HPLC results obtained for the monomeric fraction have been enlarged on the left-hand side. Peaks 1–3 were recovered and analyzed by ESI-MS. The structures of peaks 1–3 are shown on the right-hand side. Peaks 1 and 2 (asterisks) accumulated in the cyslabdan-treated MRSA.
Figure Legend Snippet: HPLC analysis of the peptidoglycan composition of cyslabdan-treated MRSA. (A) HPLC analysis of the muropeptides extracted from MRSA peptidoglycans. Peptidoglycans were prepared from MRSA in the presence or absence of cyslabdan (4 µg/mL). After treating the peptidoglycans with mutanolysin and sodium tetrahydroborate, the resultant monomer-, dimer-, trimer-, and oligomer-type muropeptide products were analyzed by HPLC under the established conditions using an ODS column. The upper chromatogram indicates the results obtained for control MRSA, whereas the lower chromatogram indicates those obtained for cyslabdan-treated MRSA. (B) Structures of monomer-type muropeptides identified by ESI-MS. The HPLC results obtained for the monomeric fraction have been enlarged on the left-hand side. Peaks 1–3 were recovered and analyzed by ESI-MS. The structures of peaks 1–3 are shown on the right-hand side. Peaks 1 and 2 (asterisks) accumulated in the cyslabdan-treated MRSA.

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

8) Product Images from "β-Mannanase-catalyzed synthesis of alkyl mannooligosides"

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides

Journal: Applied Microbiology and Biotechnology

doi: 10.1007/s00253-018-8997-2

Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)
Figure Legend Snippet: Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

9) Product Images from "Production of Group Specific Monoclonal Antibody to Aflatoxins and its Application to Enzyme-linked Immunosorbent Assay"

Article Title: Production of Group Specific Monoclonal Antibody to Aflatoxins and its Application to Enzyme-linked Immunosorbent Assay

Journal: Toxicological Research

doi: 10.5487/TR.2011.27.2.125

HPLC chromatogram and UV scanning spectrum of aflatoxin B1 (A) and aflatoxin B1-CMO (B).
Figure Legend Snippet: HPLC chromatogram and UV scanning spectrum of aflatoxin B1 (A) and aflatoxin B1-CMO (B).

Techniques Used: High Performance Liquid Chromatography

10) Product Images from "Preparation and Antioxidant Activity of Ethyl-Linked Anthocyanin-Flavanol Pigments from Model Wine Solutions"

Article Title: Preparation and Antioxidant Activity of Ethyl-Linked Anthocyanin-Flavanol Pigments from Model Wine Solutions

Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

doi: 10.3390/molecules23051066

Preparative-HPLC chromatograms of six ethyl-linked anthocyanin-flavanol pigments. ( A ) 1: malvidin-3-glucoside-ethyl-epicatechin ( S ); 2: malvidin-3-glucoside-ethyl-epicatechin ( R ). ( B ) 1: cyanidin-3-glucoside-ethyl-epicatechin ( S ); 2: cyanidin-3-glucoside-ethyl-epicatechin ( R ). ( C ) 1: peonidin-3-glucoside-ethyl-epicatechin ( S ); 2: peonidin-3-glucoside-ethyl-epicatechin ( R ).
Figure Legend Snippet: Preparative-HPLC chromatograms of six ethyl-linked anthocyanin-flavanol pigments. ( A ) 1: malvidin-3-glucoside-ethyl-epicatechin ( S ); 2: malvidin-3-glucoside-ethyl-epicatechin ( R ). ( B ) 1: cyanidin-3-glucoside-ethyl-epicatechin ( S ); 2: cyanidin-3-glucoside-ethyl-epicatechin ( R ). ( C ) 1: peonidin-3-glucoside-ethyl-epicatechin ( S ); 2: peonidin-3-glucoside-ethyl-epicatechin ( R ).

Techniques Used: High Performance Liquid Chromatography

Chromatograms of HPLC analysis of the reaction between cyanidin-3-glucoside and (–)-epicatechin at 0 h ( a ), 2 h ( b ), 224 h ( c ), 336 h ( d ); Chromatograms of HPLC analysis of the reaction between malvidin-3-glucoside and (–)-epicatechin at 0h ( e ), 2 h ( f ), 224 h ( g ), 336 h ( h ); Chromatograms of HPLC analysis of the reaction between peonidin-3-glucoside and (–)-epicatechin at 0 h ( i ), 2 h ( j ), 224 h ( k ), 336 h ( l ).
Figure Legend Snippet: Chromatograms of HPLC analysis of the reaction between cyanidin-3-glucoside and (–)-epicatechin at 0 h ( a ), 2 h ( b ), 224 h ( c ), 336 h ( d ); Chromatograms of HPLC analysis of the reaction between malvidin-3-glucoside and (–)-epicatechin at 0h ( e ), 2 h ( f ), 224 h ( g ), 336 h ( h ); Chromatograms of HPLC analysis of the reaction between peonidin-3-glucoside and (–)-epicatechin at 0 h ( i ), 2 h ( j ), 224 h ( k ), 336 h ( l ).

Techniques Used: High Performance Liquid Chromatography

11) Product Images from "β-Mannanase-catalyzed synthesis of alkyl mannooligosides"

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides

Journal: Applied Microbiology and Biotechnology

doi: 10.1007/s00253-018-8997-2

Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)
Figure Legend Snippet: Chromatogram showing separation of hexyl mannooligosides with reversed-phase HPLC using a C 18 column. The two inserts show MALDI-TOF MS spectra with peak mass identifications corresponding to monoisotopic sodium adduct masses of hexyl-M 3 (theoretical mass 611.25) and hexyl-M 2 (theoretical mass 449.20)

Techniques Used: High Performance Liquid Chromatography, Mass Spectrometry

Related Articles

High Performance Liquid Chromatography:

Article Title: Benzanthric Acid, a Novel Metabolite From Streptomyces albus Del14 Expressing the Nybomycin Gene Cluster
Article Snippet: .. Resulting fractions were analyzed by LC-MS and those containing benzanthric acid were further separated by preparative HPLC (Waters 2545 Binary Gradient module, Waters, Milford, MA, USA) using a Nucleodur® C18 HTec column (5 μm, 250 × 21 mm, Macherey-Nagel, Düren, Germany) with a linear gradient of 0.1% formic acid solution in methanol against 0.1% formic acid solution in water, yielding 5 mg of benzanthric acid. .. UV spectra were recorded with a PAD detector (Photodiode Array Detector, Waters 2998, Waters, Milford, MA, USA).

Article Title: Uncovering production of specialized metabolites by Streptomyces argillaceus: Activation of cryptic biosynthesis gene clusters using nutritional and genetic approaches
Article Snippet: .. The germicidins were purified by preparative HPLC using a SunFire C18 column (10 μm, 10 x 250 mm, Waters). ..

Article Title: Identification of the Potential Biological Preservative Tetramycin A-Producing Strain and Enhancing Its Production
Article Snippet: .. The different fractions were obtained by Waters’ preparative HPLC and termed as I–VII fractions ( ). ..

Article Title: The Nonantibiotic Small Molecule Cyslabdan Enhances the Potency of ?-Lactams against MRSA by Inhibiting Pentaglycine Interpeptide Bridge Synthesis
Article Snippet: .. To identify these muropeptides, the samples were collected by preparative HPLC under the same conditions and were then desalted with a Sep-Pak cartridge (Waters, Milford, MA, USA) and analyzed via ESI-MS. ..

Article Title: Preparation and Antioxidant Activity of Ethyl-Linked Anthocyanin-Flavanol Pigments from Model Wine Solutions
Article Snippet: .. The further purification of malvidin-3-glucoside and peonidin-3-glucoside was performed using preparative HPLC, with the mobile phase consisting of 0.2% formic acid-water (solvent A) and 0.2% formic acid-acetonitrile (solvent B). ..

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides
Article Snippet: .. Hexyl mannooligosides were synthesized from β-mannan and 1-hexanol and purified using preparative HPLC. ..

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides
Article Snippet: .. The obtained hexyl mannooligosides were then purified with preparative HPLC using a Waters Symmetry C18 Prep column using a 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA), with a 10–45% gradient of ACN versus 0.1% formic acid in water over 9 min at room temperature, followed by a steeper gradient of 45–90% ACN over 2 min and, finally, a wash step with 90% ACN for 2 min. Fractions were collected during the entire separation and analyzed with MALDI-TOF MS to identify fractions containing hexyl mannooligosides. ..

Purification:

Article Title: Uncovering production of specialized metabolites by Streptomyces argillaceus: Activation of cryptic biosynthesis gene clusters using nutritional and genetic approaches
Article Snippet: .. The germicidins were purified by preparative HPLC using a SunFire C18 column (10 μm, 10 x 250 mm, Waters). ..

Article Title: Preparation and Antioxidant Activity of Ethyl-Linked Anthocyanin-Flavanol Pigments from Model Wine Solutions
Article Snippet: .. The further purification of malvidin-3-glucoside and peonidin-3-glucoside was performed using preparative HPLC, with the mobile phase consisting of 0.2% formic acid-water (solvent A) and 0.2% formic acid-acetonitrile (solvent B). ..

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides
Article Snippet: .. Hexyl mannooligosides were synthesized from β-mannan and 1-hexanol and purified using preparative HPLC. ..

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides
Article Snippet: .. The obtained hexyl mannooligosides were then purified with preparative HPLC using a Waters Symmetry C18 Prep column using a 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA), with a 10–45% gradient of ACN versus 0.1% formic acid in water over 9 min at room temperature, followed by a steeper gradient of 45–90% ACN over 2 min and, finally, a wash step with 90% ACN for 2 min. Fractions were collected during the entire separation and analyzed with MALDI-TOF MS to identify fractions containing hexyl mannooligosides. ..

Liquid Chromatography with Mass Spectroscopy:

Article Title: Benzanthric Acid, a Novel Metabolite From Streptomyces albus Del14 Expressing the Nybomycin Gene Cluster
Article Snippet: .. Resulting fractions were analyzed by LC-MS and those containing benzanthric acid were further separated by preparative HPLC (Waters 2545 Binary Gradient module, Waters, Milford, MA, USA) using a Nucleodur® C18 HTec column (5 μm, 250 × 21 mm, Macherey-Nagel, Düren, Germany) with a linear gradient of 0.1% formic acid solution in methanol against 0.1% formic acid solution in water, yielding 5 mg of benzanthric acid. .. UV spectra were recorded with a PAD detector (Photodiode Array Detector, Waters 2998, Waters, Milford, MA, USA).

Mass Spectrometry:

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides
Article Snippet: .. The obtained hexyl mannooligosides were then purified with preparative HPLC using a Waters Symmetry C18 Prep column using a 1260 Infinity system (Agilent Technologies, Santa Clara, CA, USA), with a 10–45% gradient of ACN versus 0.1% formic acid in water over 9 min at room temperature, followed by a steeper gradient of 45–90% ACN over 2 min and, finally, a wash step with 90% ACN for 2 min. Fractions were collected during the entire separation and analyzed with MALDI-TOF MS to identify fractions containing hexyl mannooligosides. ..

Synthesized:

Article Title: β-Mannanase-catalyzed synthesis of alkyl mannooligosides
Article Snippet: .. Hexyl mannooligosides were synthesized from β-mannan and 1-hexanol and purified using preparative HPLC. ..

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    Waters Corporation preparative reverse phase high performance liquid chromatography hplc
    Two-step downstream purification scheme for <t>rhTIMP-2–</t> 6XHis. (A) Immobilized metal ion affinity chromatography (HisTrap column) for batchwise elution yields a single sharp elution peak using a step gradient of 20 mM (nonspecific) and 250 mM (specific, buffer B) elution. (B) Preparative reverse phase <t>HPLC</t> also yields a single Gaussian peak. The blue line indicates the % solvent B (acetonitrile, 0.1% TFA) composition of the mobile phase, with initial isocratic elution using 10% solvent B until 2 min and then a 10 to 60% solvent B gradient between 2 and 6 min. The column was then washed with a gradient of 60 to 100% solvent B between 6 and 10 min, before reequilibration with 10% solvent B.
    Preparative Reverse Phase High Performance Liquid Chromatography Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Waters Corporation semi preparative hplc
    ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] <t>10a</t> from crude reaction mixture by semi-preparative <t>HPLC</t> (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .
    Semi Preparative Hplc, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Waters Corporation semi preparative strong anion exchange sax high performance liquid chromatography
    Chromatograms of heparin lyases produced oligosaccharides for heparin mapping. <t>SAX-HPLC</t> chromatograms are: a. heparin lyase 1 treated heparin; b. heparin lyase 2-treated heparin; and c. heparin lyase 3 treated heparin. The structures determined for each peak are: 2a ΔUA-GlcNAc, 2b ΔUA-GlcNS6S, 2c ΔUA2S-GlcNS, 2d ΔUA2S-GlcNS6S, 2e ΔUA-GlcNS, 2f ΔUA-GlcNAc6S, 2g ΔUA2S-GlcNAc, 2h ΔUA2S-GlcNAc6S, 4a ΔUA2S-GlcNS-IdoA2S-GlcNS, 4b ΔUA2S-GlcNS6S-IdoA2S-GlcNS, 4c ΔUA2S-GlcNS6S-GlcA-GlcNS6S, 4d ΔUA2S-GlcNS6S-IdoA2S-GlcNS6S, 4e ΔUA-Gal-Gal-Xyl- O -CH 2 CONHCH 2 COOH, 4f ΔUA-GlcNAc6S-GlcA-GlcNS3S, 4g ΔUA-GlcNAc6S-GlcA-GlcNS3S6S, 4h ΔUA-GlcNS6S-GlcA-GlcNS3S6S, 4i ΔUA-Gal-Gal-Xyl- O -Ser, 6a ΔUA2S-GlcNS6S-IdoA-GlcNAc6S-GlcA-GlcNS3S6S.
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    Waters Corporation hplc preparative system
    Influence of MeCN amount on <t>HPPTCA</t> release from plasma proteins, expressed as a peak height of HPPTCA. Samples were analyzed according to a previously published <t>HPLC-UV</t> assay [ 21 ].
    Hplc Preparative System, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 80/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Two-step downstream purification scheme for rhTIMP-2– 6XHis. (A) Immobilized metal ion affinity chromatography (HisTrap column) for batchwise elution yields a single sharp elution peak using a step gradient of 20 mM (nonspecific) and 250 mM (specific, buffer B) elution. (B) Preparative reverse phase HPLC also yields a single Gaussian peak. The blue line indicates the % solvent B (acetonitrile, 0.1% TFA) composition of the mobile phase, with initial isocratic elution using 10% solvent B until 2 min and then a 10 to 60% solvent B gradient between 2 and 6 min. The column was then washed with a gradient of 60 to 100% solvent B between 6 and 10 min, before reequilibration with 10% solvent B.

    Journal: Biochemistry

    Article Title: Tissue Inhibitor of Metalloprotease-2 (TIMP-2): Bioprocess Development, Physicochemical, Biochemical, and Biological Characterization of Highly Expressed Recombinant Protein

    doi: 10.1021/acs.biochem.7b00700

    Figure Lengend Snippet: Two-step downstream purification scheme for rhTIMP-2– 6XHis. (A) Immobilized metal ion affinity chromatography (HisTrap column) for batchwise elution yields a single sharp elution peak using a step gradient of 20 mM (nonspecific) and 250 mM (specific, buffer B) elution. (B) Preparative reverse phase HPLC also yields a single Gaussian peak. The blue line indicates the % solvent B (acetonitrile, 0.1% TFA) composition of the mobile phase, with initial isocratic elution using 10% solvent B until 2 min and then a 10 to 60% solvent B gradient between 2 and 6 min. The column was then washed with a gradient of 60 to 100% solvent B between 6 and 10 min, before reequilibration with 10% solvent B.

    Article Snippet: Purified rhTIMP-2-6XHis was further purified by preparative reverse phase high-performance liquid chromatography (HPLC) using a Waters Delta Prep HPLC instrument.

    Techniques: Purification, Affinity Chromatography, High Performance Liquid Chromatography

    Analysis of two-step downstream purification of rhTIMP-2– 6XHis following bioprocess scale expression. Samples from the bioprocess purification were analyzed as shown in the (A) PageBlue Protein-stained SDS−PAGE gel of the fractions obtained from the IMAC (HisTrap column) purification step. Lane M contained the molecular weight standards (SeeBlue Plus 2 Prestained Standards, Invitrogen, catalog no. LC 5925). Lane CM contained the starting condition medium sample obtained from the HEK-293-F suspension culture. Lane FT contained the flow-through (unbound) fraction obtained during sample loading. Lane NSE contained the nonspecific elution obtained during step gradient elution with 20 mM imidazole. Lane SE contained the specific elution fraction obtained following 250 mM imidazole step elution. (B) PageBlue stained SDS−PAGE gel showing that the rhTIMP-2-6XHis-containing fractions from the IMAC (HisTrap) purification contain a predominant 22 kDa band with minor higher-molecular weight contaminants. The reverse phase HPLC purification effectively removed these higher-molecular weight contaminants, resulting in a single 22 kDa band with > 95% purity as estimated by densitometry using a Bio-Rad ChemiDoc XRS + instrument with Image Lab software. (C) Western Blot analysis of the IMAC fractions using anti-TIMP-2 (top) anti-6XHis tag (bottom) antibodies. The lanes are labeled the same as in panel A.

    Journal: Biochemistry

    Article Title: Tissue Inhibitor of Metalloprotease-2 (TIMP-2): Bioprocess Development, Physicochemical, Biochemical, and Biological Characterization of Highly Expressed Recombinant Protein

    doi: 10.1021/acs.biochem.7b00700

    Figure Lengend Snippet: Analysis of two-step downstream purification of rhTIMP-2– 6XHis following bioprocess scale expression. Samples from the bioprocess purification were analyzed as shown in the (A) PageBlue Protein-stained SDS−PAGE gel of the fractions obtained from the IMAC (HisTrap column) purification step. Lane M contained the molecular weight standards (SeeBlue Plus 2 Prestained Standards, Invitrogen, catalog no. LC 5925). Lane CM contained the starting condition medium sample obtained from the HEK-293-F suspension culture. Lane FT contained the flow-through (unbound) fraction obtained during sample loading. Lane NSE contained the nonspecific elution obtained during step gradient elution with 20 mM imidazole. Lane SE contained the specific elution fraction obtained following 250 mM imidazole step elution. (B) PageBlue stained SDS−PAGE gel showing that the rhTIMP-2-6XHis-containing fractions from the IMAC (HisTrap) purification contain a predominant 22 kDa band with minor higher-molecular weight contaminants. The reverse phase HPLC purification effectively removed these higher-molecular weight contaminants, resulting in a single 22 kDa band with > 95% purity as estimated by densitometry using a Bio-Rad ChemiDoc XRS + instrument with Image Lab software. (C) Western Blot analysis of the IMAC fractions using anti-TIMP-2 (top) anti-6XHis tag (bottom) antibodies. The lanes are labeled the same as in panel A.

    Article Snippet: Purified rhTIMP-2-6XHis was further purified by preparative reverse phase high-performance liquid chromatography (HPLC) using a Waters Delta Prep HPLC instrument.

    Techniques: Purification, Expressing, Staining, SDS Page, Molecular Weight, Flow Cytometry, High Performance Liquid Chromatography, Software, Western Blot, Labeling

    ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] 10a from crude reaction mixture by semi-preparative HPLC (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .

    Journal: Molecules

    Article Title: A Promising PET Tracer for Imaging of α7 Nicotinic Acetylcholine Receptors in the Brain: Design, Synthesis, and in Vivo Evaluation of a Dibenzothiophene-Based Radioligand

    doi: 10.3390/molecules201018387

    Figure Lengend Snippet: ( a ) Representative radio- and UV-chromatograms obtained for isolation of [ 18 F] 10a from crude reaction mixture by semi-preparative HPLC (Reprosil-Pur C18-AQ column, 35% MeCN/H 2 O/0.05% TFA, Flow rate: 10 mL∙min −1 ); (b ) Analytical radio-chromatogram (top) and UV-chromatogram (bottom) of purified [ 18 F] 10a spiked with the reference compound 10a .

    Article Snippet: After isolation via semi-preparative HPLC, [18 F] 10a was trapped on a pre-conditioned Sep-Pak® (Waters, Milford, MA, USA) C18 light cartridge, and formulated in isotonic saline containing 10% of EtOH (v /v ).

    Techniques: Isolation, High Performance Liquid Chromatography, Flow Cytometry, Purification

    Chromatograms of heparin lyases produced oligosaccharides for heparin mapping. SAX-HPLC chromatograms are: a. heparin lyase 1 treated heparin; b. heparin lyase 2-treated heparin; and c. heparin lyase 3 treated heparin. The structures determined for each peak are: 2a ΔUA-GlcNAc, 2b ΔUA-GlcNS6S, 2c ΔUA2S-GlcNS, 2d ΔUA2S-GlcNS6S, 2e ΔUA-GlcNS, 2f ΔUA-GlcNAc6S, 2g ΔUA2S-GlcNAc, 2h ΔUA2S-GlcNAc6S, 4a ΔUA2S-GlcNS-IdoA2S-GlcNS, 4b ΔUA2S-GlcNS6S-IdoA2S-GlcNS, 4c ΔUA2S-GlcNS6S-GlcA-GlcNS6S, 4d ΔUA2S-GlcNS6S-IdoA2S-GlcNS6S, 4e ΔUA-Gal-Gal-Xyl- O -CH 2 CONHCH 2 COOH, 4f ΔUA-GlcNAc6S-GlcA-GlcNS3S, 4g ΔUA-GlcNAc6S-GlcA-GlcNS3S6S, 4h ΔUA-GlcNS6S-GlcA-GlcNS3S6S, 4i ΔUA-Gal-Gal-Xyl- O -Ser, 6a ΔUA2S-GlcNS6S-IdoA-GlcNAc6S-GlcA-GlcNS3S6S.

    Journal: Journal of medicinal chemistry

    Article Title: Heparin mapping using heparin lyases and the generation of a novel low molecular weight heparin

    doi: 10.1021/jm101381k

    Figure Lengend Snippet: Chromatograms of heparin lyases produced oligosaccharides for heparin mapping. SAX-HPLC chromatograms are: a. heparin lyase 1 treated heparin; b. heparin lyase 2-treated heparin; and c. heparin lyase 3 treated heparin. The structures determined for each peak are: 2a ΔUA-GlcNAc, 2b ΔUA-GlcNS6S, 2c ΔUA2S-GlcNS, 2d ΔUA2S-GlcNS6S, 2e ΔUA-GlcNS, 2f ΔUA-GlcNAc6S, 2g ΔUA2S-GlcNAc, 2h ΔUA2S-GlcNAc6S, 4a ΔUA2S-GlcNS-IdoA2S-GlcNS, 4b ΔUA2S-GlcNS6S-IdoA2S-GlcNS, 4c ΔUA2S-GlcNS6S-GlcA-GlcNS6S, 4d ΔUA2S-GlcNS6S-IdoA2S-GlcNS6S, 4e ΔUA-Gal-Gal-Xyl- O -CH 2 CONHCH 2 COOH, 4f ΔUA-GlcNAc6S-GlcA-GlcNS3S, 4g ΔUA-GlcNAc6S-GlcA-GlcNS3S6S, 4h ΔUA-GlcNS6S-GlcA-GlcNS3S6S, 4i ΔUA-Gal-Gal-Xyl- O -Ser, 6a ΔUA2S-GlcNS6S-IdoA-GlcNAc6S-GlcA-GlcNS3S6S.

    Article Snippet: The resulting mixture was fractionated on a 20 × 250 mm semi-preparative strong anion exchange (SAX)-high performance liquid chromatography (HPLC) column (Waters Spherisorb S5) eluted with a salt gradient (see below) over 60 min at a flow rate of 4.0 mL/min with absorbance detected at 232 nm.

    Techniques: Produced, High Performance Liquid Chromatography

    Heparin lyase 1 and heparin lyase 2 action pattern studies using decasaccharide 1 0a as a model substrate. 1 0a ΔUA2S-[-GlcNS6S-IdoA2S-] 4 -GlcNS6S. SAX-HPLC chromatograms of a digestion time course are shown for a. heparin lyase 1 and b. for heparin lyase 2. The red arrows indicate increasing digestion time. PAGE (22% total acrylamide) analyses of 1 0a treated by heparin lyase 1 and heparin lyase 2 are shown in c. where lane 1 is 4d ; lane 2 is 8b ΔUA2S-[-GlcNS6S-IdoA2S-] 3 -GlcNS6S; lane 3 through lane 8 are 1 0a incubated with heparin lyase 1 from time-point 0 to time-point 5; lane 9 through lane 13 are 1 0a treated by heparin lyase 2 from time-point 0 to time-point 5; lane 8 and lane 13 are 1 0a exhaustively digested by heparin lyase 1 and heparin lyase 2, respectively. The mole percent of each size product (indicated with different symbols) is plotted as a function of percent reaction completion in d. for heparin lyase 1 (dotted lines) and heparin lyase 2 (solid lines).

    Journal: Journal of medicinal chemistry

    Article Title: Heparin mapping using heparin lyases and the generation of a novel low molecular weight heparin

    doi: 10.1021/jm101381k

    Figure Lengend Snippet: Heparin lyase 1 and heparin lyase 2 action pattern studies using decasaccharide 1 0a as a model substrate. 1 0a ΔUA2S-[-GlcNS6S-IdoA2S-] 4 -GlcNS6S. SAX-HPLC chromatograms of a digestion time course are shown for a. heparin lyase 1 and b. for heparin lyase 2. The red arrows indicate increasing digestion time. PAGE (22% total acrylamide) analyses of 1 0a treated by heparin lyase 1 and heparin lyase 2 are shown in c. where lane 1 is 4d ; lane 2 is 8b ΔUA2S-[-GlcNS6S-IdoA2S-] 3 -GlcNS6S; lane 3 through lane 8 are 1 0a incubated with heparin lyase 1 from time-point 0 to time-point 5; lane 9 through lane 13 are 1 0a treated by heparin lyase 2 from time-point 0 to time-point 5; lane 8 and lane 13 are 1 0a exhaustively digested by heparin lyase 1 and heparin lyase 2, respectively. The mole percent of each size product (indicated with different symbols) is plotted as a function of percent reaction completion in d. for heparin lyase 1 (dotted lines) and heparin lyase 2 (solid lines).

    Article Snippet: The resulting mixture was fractionated on a 20 × 250 mm semi-preparative strong anion exchange (SAX)-high performance liquid chromatography (HPLC) column (Waters Spherisorb S5) eluted with a salt gradient (see below) over 60 min at a flow rate of 4.0 mL/min with absorbance detected at 232 nm.

    Techniques: High Performance Liquid Chromatography, Polyacrylamide Gel Electrophoresis, Incubation

    Influence of MeCN amount on HPPTCA release from plasma proteins, expressed as a peak height of HPPTCA. Samples were analyzed according to a previously published HPLC-UV assay [ 21 ].

    Journal: International Journal of Molecular Sciences

    Article Title: 2-(3-Hydroxy-5-phosphonooxymethyl-2-methyl-4-pyridyl)-1,3-thiazolidine-4-carboxylic Acid, Novel Metabolite of Pyridoxal 5′-Phosphate and Cysteine Is Present in Human Plasma—Chromatographic Investigations

    doi: 10.3390/ijms21103548

    Figure Lengend Snippet: Influence of MeCN amount on HPPTCA release from plasma proteins, expressed as a peak height of HPPTCA. Samples were analyzed according to a previously published HPLC-UV assay [ 21 ].

    Article Snippet: Purification of HPPTCA was performed with the use of an HPLC preparative system from Waters (USA) equipped with a 2545 binary gradient pump and a 2998 DAD detector.

    Techniques: High Performance Liquid Chromatography, UV Assay

    Influence of temperature on HPPTCA stability, expressed as a peak height of HPPTCA. Samples were assayed according to a previously published procedure based on HPLC-UV measurements [ 21 ].

    Journal: International Journal of Molecular Sciences

    Article Title: 2-(3-Hydroxy-5-phosphonooxymethyl-2-methyl-4-pyridyl)-1,3-thiazolidine-4-carboxylic Acid, Novel Metabolite of Pyridoxal 5′-Phosphate and Cysteine Is Present in Human Plasma—Chromatographic Investigations

    doi: 10.3390/ijms21103548

    Figure Lengend Snippet: Influence of temperature on HPPTCA stability, expressed as a peak height of HPPTCA. Samples were assayed according to a previously published procedure based on HPLC-UV measurements [ 21 ].

    Article Snippet: Purification of HPPTCA was performed with the use of an HPLC preparative system from Waters (USA) equipped with a 2545 binary gradient pump and a 2998 DAD detector.

    Techniques: High Performance Liquid Chromatography

    Stability of HPPTCA in human plasma spiked with the analyte (100 µmol L −1 ) at room temperature, expressed as a peak area. Samples were processed according to previously published methods based on HPLC-UV [ 21 , 22 ].

    Journal: International Journal of Molecular Sciences

    Article Title: 2-(3-Hydroxy-5-phosphonooxymethyl-2-methyl-4-pyridyl)-1,3-thiazolidine-4-carboxylic Acid, Novel Metabolite of Pyridoxal 5′-Phosphate and Cysteine Is Present in Human Plasma—Chromatographic Investigations

    doi: 10.3390/ijms21103548

    Figure Lengend Snippet: Stability of HPPTCA in human plasma spiked with the analyte (100 µmol L −1 ) at room temperature, expressed as a peak area. Samples were processed according to previously published methods based on HPLC-UV [ 21 , 22 ].

    Article Snippet: Purification of HPPTCA was performed with the use of an HPLC preparative system from Waters (USA) equipped with a 2545 binary gradient pump and a 2998 DAD detector.

    Techniques: High Performance Liquid Chromatography