Structured Review

Phenomenex preparative hplc
Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) <t>HPLC</t> chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: <t>Phenomenex</t> Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Images

1) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

2) Product Images from "Structure of ?-conotoxin BuIA: influences of disulfide connectivity on structural dynamics"

Article Title: Structure of ?-conotoxin BuIA: influences of disulfide connectivity on structural dynamics

Journal: BMC Structural Biology

doi: 10.1186/1472-6807-7-28

Disulfide isomers of BuIA. (A) A schematic representation of the ribbon, globular and beads disulfide connectivities of BuIA, a conotoxin having the unique 4/4 loop spacing. (B) HPLC traces of ribbon and globular BuIA. Analytical HPLC was carried out using a C 18 Phenomenex analytical column (250 × 4.6 mm) with 90% acetonitrile and 0.043% trifluroacetic acid as the eluting solvent B and 1% B per minute as the gradient. Ribbon BuIA elutes earlier than the globular form but has a slightly broadened peak under the conditions utilized.
Figure Legend Snippet: Disulfide isomers of BuIA. (A) A schematic representation of the ribbon, globular and beads disulfide connectivities of BuIA, a conotoxin having the unique 4/4 loop spacing. (B) HPLC traces of ribbon and globular BuIA. Analytical HPLC was carried out using a C 18 Phenomenex analytical column (250 × 4.6 mm) with 90% acetonitrile and 0.043% trifluroacetic acid as the eluting solvent B and 1% B per minute as the gradient. Ribbon BuIA elutes earlier than the globular form but has a slightly broadened peak under the conditions utilized.

Techniques Used: High Performance Liquid Chromatography

3) Product Images from "Post-synthetic and site-specific modification of endocyclic nitrogen atoms of purines in DNA and its potential for biological and structural studies"

Article Title: Post-synthetic and site-specific modification of endocyclic nitrogen atoms of purines in DNA and its potential for biological and structural studies

Journal: Nucleic Acids Research

doi: 10.1093/nar/gki315

( a ) The converted modified nucleosides [N1-hydroxy-2′-deoxyinsoine 6 (Rt = 4.2 min), N1-methyl-2′-deoxyinosine 4 (Rt = 10.3 min) and 2′-deoxyoxanosine 7 (Rt = 14.8 min)] can be easily separated from the starting material 2 (Rt = 23.5 min) by HPLC. HPLC conditions: Atlantis dC 18 column (5 μm, 250 × 4.6 mm); mobile phase A was water; mobile phase B was CH 3 CN; and mobile phase C was 10 mM KH 2 PO 4 , pH 6.52. The mobile phase C was kept constant at 20%, while mobile phase B was increased from 5 to 30% over a period of 30 min. ( b ) These modified nucleosides have distinct UV spectra (λ max ): N1-hydroxy-2′-deoxyinosine 6 (227, 252 and 294.6 nm); N1-methyl-2′-deoxyinosine 4 (265 nm); 2′-deoxyoxanosine 7 (247 and 285 nm) and N1-dinitrophenyl-2′-deoxyinosine 2 (245 nm) in H 2 O/CH 3 OH (1:1).
Figure Legend Snippet: ( a ) The converted modified nucleosides [N1-hydroxy-2′-deoxyinsoine 6 (Rt = 4.2 min), N1-methyl-2′-deoxyinosine 4 (Rt = 10.3 min) and 2′-deoxyoxanosine 7 (Rt = 14.8 min)] can be easily separated from the starting material 2 (Rt = 23.5 min) by HPLC. HPLC conditions: Atlantis dC 18 column (5 μm, 250 × 4.6 mm); mobile phase A was water; mobile phase B was CH 3 CN; and mobile phase C was 10 mM KH 2 PO 4 , pH 6.52. The mobile phase C was kept constant at 20%, while mobile phase B was increased from 5 to 30% over a period of 30 min. ( b ) These modified nucleosides have distinct UV spectra (λ max ): N1-hydroxy-2′-deoxyinosine 6 (227, 252 and 294.6 nm); N1-methyl-2′-deoxyinosine 4 (265 nm); 2′-deoxyoxanosine 7 (247 and 285 nm) and N1-dinitrophenyl-2′-deoxyinosine 2 (245 nm) in H 2 O/CH 3 OH (1:1).

Techniques Used: Modification, High Performance Liquid Chromatography

4) Product Images from "Structurally divergent dynamic combinatorial chemistry on racemic mixtures"

Article Title: Structurally divergent dynamic combinatorial chemistry on racemic mixtures

Journal: Nature Communications

doi: 10.1038/s41467-020-17321-2

Heterochiral DCL. a Reverse-phase HPLC analysis at 389 nm of S,S - 1 : R,R - 2 (1:1 molar ratio, 5 mM total concentration) library in the presence of 1 M NaNO 3 ; b schematic representation of equilibria showing the pathway for the formation of Cat II RSRR and c table containing the yield for each species in DCL; the integrations were done in triplicate and the RMSD is reported in parenthesis along with the value.
Figure Legend Snippet: Heterochiral DCL. a Reverse-phase HPLC analysis at 389 nm of S,S - 1 : R,R - 2 (1:1 molar ratio, 5 mM total concentration) library in the presence of 1 M NaNO 3 ; b schematic representation of equilibria showing the pathway for the formation of Cat II RSRR and c table containing the yield for each species in DCL; the integrations were done in triplicate and the RMSD is reported in parenthesis along with the value.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay

5) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

6) Product Images from "Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging"

Article Title: Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging

Journal: Pharmaceuticals

doi: 10.3390/ph10040099

HPLC chromatograms of the crude 5-[ 18 F]FDR-AOA-peptide conjugates prepared using ammonium acetate (( A , C , E ); reaction conditions I) and anilinium acetate (( B , D , F , H ); reaction conditions II) as well as purified 5-[ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( G )). Reaction conditions: I: 0.2 mol·L −1 NH 4 OAc buffer, pH 4, 75 °C, 20 min; II: 0.25 mol·L −1 anilinium acetate buffer, pH 4.6, RT, 10 min, for the applied amounts of AOA-peptides, refer to Table 2 and Table 3 . HPLC conditions: column: Phenomenex Kinetex C18 100 × 4.6 mm, 2.6 μm/100 Å; eluent: 0–15 min 10 → 40% MeCN (0.1% TFA); flow rate: 1 mL/min; t r : [ 18 F]FDR-AOA-C-CPE-17-KKK ([ 18 F] 8 , ( A , B ))—11.5 min, [ 18 F]FDR-AOA-CC4P-5 ([ 18 F] 10 , ( C , D ))—10.3 min, [ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( E , F ))—7.5 min, [ 18 F]FDR-AOA-M19 ([ 18 F] 9 , ( H ))—11.6 min, side product: 5-[ 18 F]FDR—1.4 min. Radioactivity and UV- (λ = 210 nm; only ( G )) traces are shown in black and violet, respectively.
Figure Legend Snippet: HPLC chromatograms of the crude 5-[ 18 F]FDR-AOA-peptide conjugates prepared using ammonium acetate (( A , C , E ); reaction conditions I) and anilinium acetate (( B , D , F , H ); reaction conditions II) as well as purified 5-[ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( G )). Reaction conditions: I: 0.2 mol·L −1 NH 4 OAc buffer, pH 4, 75 °C, 20 min; II: 0.25 mol·L −1 anilinium acetate buffer, pH 4.6, RT, 10 min, for the applied amounts of AOA-peptides, refer to Table 2 and Table 3 . HPLC conditions: column: Phenomenex Kinetex C18 100 × 4.6 mm, 2.6 μm/100 Å; eluent: 0–15 min 10 → 40% MeCN (0.1% TFA); flow rate: 1 mL/min; t r : [ 18 F]FDR-AOA-C-CPE-17-KKK ([ 18 F] 8 , ( A , B ))—11.5 min, [ 18 F]FDR-AOA-CC4P-5 ([ 18 F] 10 , ( C , D ))—10.3 min, [ 18 F]FDR-AOA-Clone 27 ([ 18 F] 11 , ( E , F ))—7.5 min, [ 18 F]FDR-AOA-M19 ([ 18 F] 9 , ( H ))—11.6 min, side product: 5-[ 18 F]FDR—1.4 min. Radioactivity and UV- (λ = 210 nm; only ( G )) traces are shown in black and violet, respectively.

Techniques Used: High Performance Liquid Chromatography, Purification, Flow Cytometry, Radioactivity

HPLC chromatograms of the crude and purified [ 18 F] 6 (( A , B ), respectively), and 5-[ 18 F]FDR ([ 18 F] 7 , ( C )). Conditions: column: Phenomenex Luna C18(2), 250 × 4.6 mm, 5 µm; eluent: 0–5.5 min 55 → 95% MeCN; 5.5–8.0 min 95% MeCN; flow rate: 1.3 mL/min; t r = 4.5 min ([ 18 F] 6 ), t r = 2.0 min (5-[ 18 F]FDR). Radioactivity, UV- (λ = 210 nm), and ELSD-traces are shown in black, violet, and green, respectively.
Figure Legend Snippet: HPLC chromatograms of the crude and purified [ 18 F] 6 (( A , B ), respectively), and 5-[ 18 F]FDR ([ 18 F] 7 , ( C )). Conditions: column: Phenomenex Luna C18(2), 250 × 4.6 mm, 5 µm; eluent: 0–5.5 min 55 → 95% MeCN; 5.5–8.0 min 95% MeCN; flow rate: 1.3 mL/min; t r = 4.5 min ([ 18 F] 6 ), t r = 2.0 min (5-[ 18 F]FDR). Radioactivity, UV- (λ = 210 nm), and ELSD-traces are shown in black, violet, and green, respectively.

Techniques Used: High Performance Liquid Chromatography, Purification, Flow Cytometry, Radioactivity

7) Product Images from "Anti-Human Rhinoviral Activity of Polybromocatechol Compounds Isolated from the Rhodophyta, Neorhodomela aculeata"

Article Title: Anti-Human Rhinoviral Activity of Polybromocatechol Compounds Isolated from the Rhodophyta, Neorhodomela aculeata

Journal: Marine Drugs

doi: 10.3390/md10102222

RP-HPLC profile of sub-fractions of EtOAc-soluble fraction (ESF) of N. aculeata . Performed on an Agilent 1300 HPLC system fitted with a Phenomenex Luna C18 (2) column (150 × 4.6 mm, 5 μm). The elution solvent system was binary gradient of solvent A (0.02% trifluoroacetic acid (TFA) in water); solvent B (0.02% TFA in acetonitrile). The gradient flow program was, as follows: 0 min, 10% B; 30 min. The flow rate was 0.7 mL/min and detection wavelength was set at 280 nm and column temperature was 25 °C. The chromatogram of F1 was not shown.
Figure Legend Snippet: RP-HPLC profile of sub-fractions of EtOAc-soluble fraction (ESF) of N. aculeata . Performed on an Agilent 1300 HPLC system fitted with a Phenomenex Luna C18 (2) column (150 × 4.6 mm, 5 μm). The elution solvent system was binary gradient of solvent A (0.02% trifluoroacetic acid (TFA) in water); solvent B (0.02% TFA in acetonitrile). The gradient flow program was, as follows: 0 min, 10% B; 30 min. The flow rate was 0.7 mL/min and detection wavelength was set at 280 nm and column temperature was 25 °C. The chromatogram of F1 was not shown.

Techniques Used: High Performance Liquid Chromatography, Flow Cytometry

8) Product Images from "Structurally divergent dynamic combinatorial chemistry on racemic mixtures"

Article Title: Structurally divergent dynamic combinatorial chemistry on racemic mixtures

Journal: Nature Communications

doi: 10.1038/s41467-020-17321-2

Homochiral DCL. a Reverse-phase HPLC analysis at 389 nm of R,R - 1 : R,R - 2 (1:1 molar ratio, 5 mM total concentration) library in the presence of 1 M NaNO 3 ; b schematic representation of equilibria showing the pathway for the formation of Cat I RRRR and c table containing the yields for each species in DCL; the integrations were done in triplicate and the RMSD is reported in parenthesis along with the value.
Figure Legend Snippet: Homochiral DCL. a Reverse-phase HPLC analysis at 389 nm of R,R - 1 : R,R - 2 (1:1 molar ratio, 5 mM total concentration) library in the presence of 1 M NaNO 3 ; b schematic representation of equilibria showing the pathway for the formation of Cat I RRRR and c table containing the yields for each species in DCL; the integrations were done in triplicate and the RMSD is reported in parenthesis along with the value.

Techniques Used: High Performance Liquid Chromatography, Concentration Assay

9) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

10) Product Images from "Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model"

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

Journal: Phytotherapy Research

doi: 10.1002/ptr.6767

Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
Figure Legend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

Techniques Used: High Performance Liquid Chromatography

Related Articles

High Performance Liquid Chromatography:

Article Title: Post-synthetic and site-specific modification of endocyclic nitrogen atoms of purines in DNA and its potential for biological and structural studies
Article Snippet: .. The last eluting peak, which had the same UV profile as reported in literature ( ) for 2′-deoxyoxanosine, was isolated on preparative HPLC as described above. ..

Article Title: Structurally divergent dynamic combinatorial chemistry on racemic mixtures
Article Snippet: .. Preparative HPLC method for DCLs of Cat II RSRR (5 mM) Column : Phenomenex Develosil Combi-RP C30, 5 × 2 cm, 5 μm; injection volume: 600 μL; flow rate: 6 mL/min; temperature: 39 °C; run time: 24 min; elution profile: See Supplementary Table . .. Analytical HPLC method for DCLs of all libraries (5 mM) Column : Restek Ultra BiPhenyl, 5 × 0.3 cm, 3 μm.

Article Title: Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging
Article Snippet: .. Further purification of the peptides was achieved by preparative HPLC on RP18 Phenomenex column (Jupiter Proteo, 250 × 15 mm, 4 μm/90 Å) using linear gradients of 10–60% B in A (A = 0.1% TFA in water; B = 0.1% TFA in acetonitrile) over 45 min and a flow rate of 6 mL min−1 . ..

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Fraction C11‐13 (190.5 mg) was subjected to preparative HPLC (Phenomenex Luna C18 column [250 × 21.2 mm, 5 μm], using isocratic elution 35, 80% CH3 CN in H2 O) to obtain two fractions (C11‐13‐1, C11‐13‐2). ..

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Fraction H11‐3 and H11‐5 were subjected to preparative HPLC (Phenomenex Luna C18 column (150 × 21.2 mm, 5 μm). ..

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Fraction C6‐7 (14.9 mg) was subjected to preparative HPLC (Phenomenex Luna C18 column (150 × 21.2 mm, 5 μm), using isocratic elution 50% CH3 CN in H2 O to afford compound 5 (tR 43 min, 2.1 mg, 80.3% area). .. Compound 10 (3.0 mg, 79.2% area) was purified from fraction C6‐11 (30.1 mg) by semi‐preparative HPLC (Phenomenex Luna 5 μm C8, 150 × 21.2 mm, 80% CH3 CN) at 27 min.

Article Title: Structure of ?-conotoxin BuIA: influences of disulfide connectivity on structural dynamics
Article Snippet: .. Preparative HPLC was carried out using a Phenomenex C18 column (250 × 21.20 mm, 10 μm). .. Analytical HPLC was performed on a Phenomenex C18 (250 × 4.6 mm, 10 μm) column.

Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model
Article Snippet: .. Fraction C11‐12 (321.4 mg) was subjected to preparative HPLC (Phenomenex Luna C18 column [250 × 21.2 mm, 5 μm], using isocratic elution 70% CH3 CN in H2 O) to purify compound 8 (tR 14 min, 26.7 mg, 86.9% area), 11 (tR 48 min, 12.3 mg, 94.3% area). .. Fraction C11‐13 (190.5 mg) was subjected to preparative HPLC (Phenomenex Luna C18 column [250 × 21.2 mm, 5 μm], using isocratic elution 35, 80% CH3 CN in H2 O) to obtain two fractions (C11‐13‐1, C11‐13‐2).

Isolation:

Article Title: Post-synthetic and site-specific modification of endocyclic nitrogen atoms of purines in DNA and its potential for biological and structural studies
Article Snippet: .. The last eluting peak, which had the same UV profile as reported in literature ( ) for 2′-deoxyoxanosine, was isolated on preparative HPLC as described above. ..

Injection:

Article Title: Structurally divergent dynamic combinatorial chemistry on racemic mixtures
Article Snippet: .. Preparative HPLC method for DCLs of Cat II RSRR (5 mM) Column : Phenomenex Develosil Combi-RP C30, 5 × 2 cm, 5 μm; injection volume: 600 μL; flow rate: 6 mL/min; temperature: 39 °C; run time: 24 min; elution profile: See Supplementary Table . .. Analytical HPLC method for DCLs of all libraries (5 mM) Column : Restek Ultra BiPhenyl, 5 × 0.3 cm, 3 μm.

Flow Cytometry:

Article Title: Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging
Article Snippet: .. Further purification of the peptides was achieved by preparative HPLC on RP18 Phenomenex column (Jupiter Proteo, 250 × 15 mm, 4 μm/90 Å) using linear gradients of 10–60% B in A (A = 0.1% TFA in water; B = 0.1% TFA in acetonitrile) over 45 min and a flow rate of 6 mL min−1 . ..

Purification:

Article Title: Convenient Preparation of 18F-Labeled Peptide Probes for Potential Claudin-4 PET Imaging
Article Snippet: .. Further purification of the peptides was achieved by preparative HPLC on RP18 Phenomenex column (Jupiter Proteo, 250 × 15 mm, 4 μm/90 Å) using linear gradients of 10–60% B in A (A = 0.1% TFA in water; B = 0.1% TFA in acetonitrile) over 45 min and a flow rate of 6 mL min−1 . ..

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    Phenomenex preparative hplc
    Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) <t>HPLC</t> chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: <t>Phenomenex</t> Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]
    Preparative Hplc, supplied by Phenomenex, used in various techniques. Bioz Stars score: 94/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

    Journal: Phytotherapy Research

    Article Title: Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model, et al. Rugosic acid A, derived from Rosa rugosa Thunb., is novel inhibitory agent for NF‐κB and IL‐6/ STAT3 axis in acute lung injury model

    doi: 10.1002/ptr.6767

    Figure Lengend Snippet: Quantitative analysis of rugosic acid A in R. rugosa extract. (a) Structure of rugosic acid A. (b) Calibration curve of rugosic aicd A standard. (c) HPLC chromatogram pattern of rugosic acid A in extract from hexan fraction of R.rugosa .; HPLC model: Agilent 1100series (Agilent), column: Phenomenex Luna C18 (5 μm, 4.6 × 250 mm), column temperature: 25°C, mobile phase: (a) H 2 O, (b) ACN, gradient: 0–0, 5–0, 45–100, 65(min)‐100(B%) [Colour figure can be viewed at wileyonlinelibrary.com ]

    Article Snippet: Fraction C6‐7 (14.9 mg) was subjected to preparative HPLC (Phenomenex Luna C18 column (150 × 21.2 mm, 5 μm), using isocratic elution 50% CH3 CN in H2 O to afford compound 5 (tR 43 min, 2.1 mg, 80.3% area).

    Techniques: High Performance Liquid Chromatography