Structured Review

MACHEREY NAGEL preparative hplc
(A) Percentage 68 Ga-radioactivity associated with plasma proteins or in solution 15 minutes after injection in mice. (B) High-performance liquid chromatography <t>(HPLC)</t> chromatograms showing the absence of metabolism and single peaks corresponding to 68 Ga-desferrioxamine B <t>(DFO)-Nsucc-PSMA</t> ( 68 Ga-4; blue) and 68 Ga-DFO- p NCS-Bn-PSMA ( 68 Ga-7; red) in the soluble blood component.
Preparative Hplc, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/preparative hplc/product/MACHEREY NAGEL
Average 93 stars, based on 42 article reviews
Price from $9.99 to $1999.99
preparative hplc - by Bioz Stars, 2020-11
93/100 stars

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1) Product Images from "Radiochemistry and Preclinical PET Imaging of 68Ga-Desferrioxamine Radiotracers Targeting Prostate-Specific Membrane Antigen"

Article Title: Radiochemistry and Preclinical PET Imaging of 68Ga-Desferrioxamine Radiotracers Targeting Prostate-Specific Membrane Antigen

Journal: Molecular Imaging

doi: 10.1177/1536012117737010

(A) Percentage 68 Ga-radioactivity associated with plasma proteins or in solution 15 minutes after injection in mice. (B) High-performance liquid chromatography (HPLC) chromatograms showing the absence of metabolism and single peaks corresponding to 68 Ga-desferrioxamine B (DFO)-Nsucc-PSMA ( 68 Ga-4; blue) and 68 Ga-DFO- p NCS-Bn-PSMA ( 68 Ga-7; red) in the soluble blood component.
Figure Legend Snippet: (A) Percentage 68 Ga-radioactivity associated with plasma proteins or in solution 15 minutes after injection in mice. (B) High-performance liquid chromatography (HPLC) chromatograms showing the absence of metabolism and single peaks corresponding to 68 Ga-desferrioxamine B (DFO)-Nsucc-PSMA ( 68 Ga-4; blue) and 68 Ga-DFO- p NCS-Bn-PSMA ( 68 Ga-7; red) in the soluble blood component.

Techniques Used: Radioactivity, Injection, Mouse Assay, High Performance Liquid Chromatography

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High Performance Liquid Chromatography:

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Article Snippet: .. The product was purified by solid phase extraction (SPE) using a Chromabond® C18 ec column (500 mg, Macherey-Nagel, Düren, Germany) followed by preparative HPLC using an EC 250/4.6 Nucleodur Sphinx RP column (250 × 4.6 mm, 5 μm particle size; Macherey-Nagel, Düren, Germany). .. The mobile phase consisted of 0.05% (v/v) formic acid in water as solvent A and acetonitrile as solvent B, with the flow rate set at 1 ml/min.

Article Title: Sfp-Type 4?-Phosphopantetheinyl Transferase Is Indispensable for Fungal Pathogenicity [W]
Article Snippet: .. Preparative HPLC (Nucleosil-7 C18; RP 18, 250 × 10 mm, 7 μm; Macherey-Nagel) with a MeCN gradient (10 to 90%) over 40 min and a flow of 20 mL/min yielded compounds 1 to 12 ( ). ..

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
Article Snippet: .. Peptides were purified by preparative and semi-preparative reversed-phase HPLC using a JASCO chromatography system (pumps PU-2080 Plus, degasser DG-2080-53, detector MD-2010 Plus) at flow rates of 10 mL min–1 and 3 mL min–1 , respectively, and a Nucleodur 100-5-C18 (250 mm by 21 mm, 5 μm) reversed-phase column from Macherey-Nagel for preparative HPLC and a Nucleodur 100-5-C18 ec, (250 mm by 10 mm, 5 μm) reversed-phase column from Macherey-Nagel for semi-preparative HPLC. .. Linear gradients of water and acetonitrile (buffer A: water, 0.1% TFA, buffer B: acetonitrile, 0.1% TFA) of 30–60% buffer B over 30 min for A peptides and 20–50% buffer B over 30 min for B peptides were used for purification.

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Article Snippet: .. Preparative HPLC was conducted on a Varian HPLC system (Pro Star 215) with a VP 250/21 Nucleosil C18 PPN column from Macherey-Nagel and monitored by UV at λ = 254 nm. .. Microwave-assisted reactions were carried out with a ‘Discover’ system from CEM Corporation.

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Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
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Article Title: Radiochemistry and Preclinical PET Imaging of 68Ga-Desferrioxamine Radiotracers Targeting Prostate-Specific Membrane Antigen
Article Snippet: .. The DFO-pNCS-Bn-PSMA ( 7 ) Urea (6) was isolated by preparative HPLC after TFA/TIS/H2 O deprotection of tris -tBu-protected urea (3). ..

Isolation:

Article Title: Radiochemistry and Preclinical PET Imaging of 68Ga-Desferrioxamine Radiotracers Targeting Prostate-Specific Membrane Antigen
Article Snippet: .. The DFO-pNCS-Bn-PSMA ( 7 ) Urea (6) was isolated by preparative HPLC after TFA/TIS/H2 O deprotection of tris -tBu-protected urea (3). ..

Flow Cytometry:

Article Title: Sfp-Type 4?-Phosphopantetheinyl Transferase Is Indispensable for Fungal Pathogenicity [W]
Article Snippet: .. Preparative HPLC (Nucleosil-7 C18; RP 18, 250 × 10 mm, 7 μm; Macherey-Nagel) with a MeCN gradient (10 to 90%) over 40 min and a flow of 20 mL/min yielded compounds 1 to 12 ( ). ..

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
Article Snippet: .. Peptides were purified by preparative and semi-preparative reversed-phase HPLC using a JASCO chromatography system (pumps PU-2080 Plus, degasser DG-2080-53, detector MD-2010 Plus) at flow rates of 10 mL min–1 and 3 mL min–1 , respectively, and a Nucleodur 100-5-C18 (250 mm by 21 mm, 5 μm) reversed-phase column from Macherey-Nagel for preparative HPLC and a Nucleodur 100-5-C18 ec, (250 mm by 10 mm, 5 μm) reversed-phase column from Macherey-Nagel for semi-preparative HPLC. .. Linear gradients of water and acetonitrile (buffer A: water, 0.1% TFA, buffer B: acetonitrile, 0.1% TFA) of 30–60% buffer B over 30 min for A peptides and 20–50% buffer B over 30 min for B peptides were used for purification.

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Article Snippet: .. Preparative HPLC was performed using VP 150/10 Nucleodur C-18, HTEC, 5 µm column (Macherey-Nagel, Düren, Germany) on a Gilson 333/334 Prep-Scale system with a flow rate of 7 mL/min, detection at 210 nm Gilson 151 (Gilson, Middleton, WI, USA), and an CH3 CN (0.005% HCO2 H)/H2 O (0.005% HCO2 H) eluent system. .. Analytical HPLC was performed using EC 150/4.6 Nucleodur C18 HTec, 5 µm column (Macherey-Nagel, Düren, Germany) on a Gilson 322 with a diode array detector Gilson 172 and evaporated light scattering detector Sedex 85 (Sedere, Oilvet, France).

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
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Chromatography:

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
Article Snippet: .. Peptides were purified by preparative and semi-preparative reversed-phase HPLC using a JASCO chromatography system (pumps PU-2080 Plus, degasser DG-2080-53, detector MD-2010 Plus) at flow rates of 10 mL min–1 and 3 mL min–1 , respectively, and a Nucleodur 100-5-C18 (250 mm by 21 mm, 5 μm) reversed-phase column from Macherey-Nagel for preparative HPLC and a Nucleodur 100-5-C18 ec, (250 mm by 10 mm, 5 μm) reversed-phase column from Macherey-Nagel for semi-preparative HPLC. .. Linear gradients of water and acetonitrile (buffer A: water, 0.1% TFA, buffer B: acetonitrile, 0.1% TFA) of 30–60% buffer B over 30 min for A peptides and 20–50% buffer B over 30 min for B peptides were used for purification.

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
Article Snippet: .. Peptide purification Peptides were purified by preparative and semi-preparative reversed-phase HPLC using a JASCO chromatography system (pumps PU-2080 Plus, degasser DG-2080-53, detector MD-2010 Plus) at flow rates of 10 mL min–1 and 3 mL min–1 , respectively, and a Nucleodur 100-5-C18 (250 mm by 21 mm, 5 μm) reversed-phase column from Macherey-Nagel for preparative HPLC and a Nucleodur 100-5-C18 ec, (250 mm by 10 mm, 5 μm) reversed-phase column from Macherey-Nagel for semi-preparative HPLC. .. Linear gradients of water and acetonitrile (buffer A: water, 0.1% TFA, buffer B: acetonitrile, 0.1% TFA) of 30–60% buffer B over 30 min for A peptides and 20–50% buffer B over 30 min for B peptides were used for purification.

Purification:

Article Title: One Pathway Is Not Enough: The Cabbage Stem Flea Beetle Psylliodes chrysocephala Uses Multiple Strategies to Overcome the Glucosinolate-Myrosinase Defense in Its Host Plants
Article Snippet: .. The product was purified by solid phase extraction (SPE) using a Chromabond® C18 ec column (500 mg, Macherey-Nagel, Düren, Germany) followed by preparative HPLC using an EC 250/4.6 Nucleodur Sphinx RP column (250 × 4.6 mm, 5 μm particle size; Macherey-Nagel, Düren, Germany). .. The mobile phase consisted of 0.05% (v/v) formic acid in water as solvent A and acetonitrile as solvent B, with the flow rate set at 1 ml/min.

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
Article Snippet: .. Peptides were purified by preparative and semi-preparative reversed-phase HPLC using a JASCO chromatography system (pumps PU-2080 Plus, degasser DG-2080-53, detector MD-2010 Plus) at flow rates of 10 mL min–1 and 3 mL min–1 , respectively, and a Nucleodur 100-5-C18 (250 mm by 21 mm, 5 μm) reversed-phase column from Macherey-Nagel for preparative HPLC and a Nucleodur 100-5-C18 ec, (250 mm by 10 mm, 5 μm) reversed-phase column from Macherey-Nagel for semi-preparative HPLC. .. Linear gradients of water and acetonitrile (buffer A: water, 0.1% TFA, buffer B: acetonitrile, 0.1% TFA) of 30–60% buffer B over 30 min for A peptides and 20–50% buffer B over 30 min for B peptides were used for purification.

Article Title: Kinetic studies on strand displacement in de novo designed parallel heterodimeric coiled coils designed parallel heterodimeric coiled coils †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc05342h
Article Snippet: .. Peptide purification Peptides were purified by preparative and semi-preparative reversed-phase HPLC using a JASCO chromatography system (pumps PU-2080 Plus, degasser DG-2080-53, detector MD-2010 Plus) at flow rates of 10 mL min–1 and 3 mL min–1 , respectively, and a Nucleodur 100-5-C18 (250 mm by 21 mm, 5 μm) reversed-phase column from Macherey-Nagel for preparative HPLC and a Nucleodur 100-5-C18 ec, (250 mm by 10 mm, 5 μm) reversed-phase column from Macherey-Nagel for semi-preparative HPLC. .. Linear gradients of water and acetonitrile (buffer A: water, 0.1% TFA, buffer B: acetonitrile, 0.1% TFA) of 30–60% buffer B over 30 min for A peptides and 20–50% buffer B over 30 min for B peptides were used for purification.

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    MACHEREY NAGEL preparative hplc
    (A) Percentage 68 Ga-radioactivity associated with plasma proteins or in solution 15 minutes after injection in mice. (B) High-performance liquid chromatography <t>(HPLC)</t> chromatograms showing the absence of metabolism and single peaks corresponding to 68 Ga-desferrioxamine B <t>(DFO)-Nsucc-PSMA</t> ( 68 Ga-4; blue) and 68 Ga-DFO- p NCS-Bn-PSMA ( 68 Ga-7; red) in the soluble blood component.
    Preparative Hplc, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative hplc/product/MACHEREY NAGEL
    Average 93 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    preparative hplc - by Bioz Stars, 2020-11
    93/100 stars
      Buy from Supplier

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    (A) Percentage 68 Ga-radioactivity associated with plasma proteins or in solution 15 minutes after injection in mice. (B) High-performance liquid chromatography (HPLC) chromatograms showing the absence of metabolism and single peaks corresponding to 68 Ga-desferrioxamine B (DFO)-Nsucc-PSMA ( 68 Ga-4; blue) and 68 Ga-DFO- p NCS-Bn-PSMA ( 68 Ga-7; red) in the soluble blood component.

    Journal: Molecular Imaging

    Article Title: Radiochemistry and Preclinical PET Imaging of 68Ga-Desferrioxamine Radiotracers Targeting Prostate-Specific Membrane Antigen

    doi: 10.1177/1536012117737010

    Figure Lengend Snippet: (A) Percentage 68 Ga-radioactivity associated with plasma proteins or in solution 15 minutes after injection in mice. (B) High-performance liquid chromatography (HPLC) chromatograms showing the absence of metabolism and single peaks corresponding to 68 Ga-desferrioxamine B (DFO)-Nsucc-PSMA ( 68 Ga-4; blue) and 68 Ga-DFO- p NCS-Bn-PSMA ( 68 Ga-7; red) in the soluble blood component.

    Article Snippet: The DFO-pNCS-Bn-PSMA ( 7 ) Urea (6) was isolated by preparative HPLC after TFA/TIS/H2 O deprotection of tris -tBu-protected urea (3).

    Techniques: Radioactivity, Injection, Mouse Assay, High Performance Liquid Chromatography