preparation human fcγriii protein  (Sino Biological)


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    Name:
    DLL1 Protein Human Recombinant
    Description:
    A DNA sequence encoding the human DLL1 NP 005609 3 Met1 Gly540 was expressed with the Fc region of human IgG1 at the C terminus
    Catalog Number:
    11635-H02H
    Price:
    None
    Category:
    recombinant protein
    Product Aliases:
    Delta Protein Human, DELTA1 Protein Human, DL1 Protein Human
    Host:
    HEK293 Cells
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    Structured Review

    Sino Biological preparation human fcγriii protein
    (A) FPOP kinetic plots of representative residues of <t>FcγRIII.</t> The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.
    A DNA sequence encoding the human DLL1 NP 005609 3 Met1 Gly540 was expressed with the Fc region of human IgG1 at the C terminus
    https://www.bioz.com/result/preparation human fcγriii protein/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    preparation human fcγriii protein - by Bioz Stars, 2021-06
    92/100 stars

    Images

    1) Product Images from "Recognition of Human IgG1 by Fcγ Receptors: Structural Insights from Hydrogen-Deuterium Exchange and Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry"

    Article Title: Recognition of Human IgG1 by Fcγ Receptors: Structural Insights from Hydrogen-Deuterium Exchange and Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry

    Journal: Biochemistry

    doi: 10.1021/acs.biochem.8b01048

    (A) FPOP kinetic plots of representative residues of FcγRIII. The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.
    Figure Legend Snippet: (A) FPOP kinetic plots of representative residues of FcγRIII. The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.

    Techniques Used: Modification

    (A) HDX kinetic plots of representative peptides of FcγRIII in the unbound (black) and bound (red) states. The peptide, its charge state, and its amino acid sequence are labeled on each panel. (B) Differential deuterium uptake percentage of FcγRIII mapped onto the crystal structure. The color scale representing the difference between bound and unbound is shown in the bottom of the panel. Mirrored views are provided for clarity.
    Figure Legend Snippet: (A) HDX kinetic plots of representative peptides of FcγRIII in the unbound (black) and bound (red) states. The peptide, its charge state, and its amino acid sequence are labeled on each panel. (B) Differential deuterium uptake percentage of FcγRIII mapped onto the crystal structure. The color scale representing the difference between bound and unbound is shown in the bottom of the panel. Mirrored views are provided for clarity.

    Techniques Used: Sequencing, Labeling

    Related Articles

    Recombinant:

    Article Title: Human Domain Antibodies to Conserved Epitopes on HER2 Potently Inhibit Growth of HER2-Overexpressing Human Breast Cancer Cells In Vitro
    Article Snippet: Cells, Proteins, Plasmids, and Other ReagentsWe purchased 293 FreeStyle (293FS) cells and protein A agarose from Thermo Fisher Scientific. .. Recombinant human (hHER2), cynomolgus (cHER2), mouse (mHER2), and rat HER2 (rHER2) and other ErbB family member proteins were purchased from Sino Biological (Beijing, China). .. The pWC1 vector for phage display and bacterial expression and the pDin1 vector used for mammalian expression were designed and generated in our laboratory.

    Article Title: Development of homogeneous plasmonic potency assay using gold nanoparticle immunocomplexes.
    Article Snippet: .. We evaluated the use of gold nanoparticles (AuNPs) platform in a homogenous assay for a potency measurement of a therapeutic monoclonal antibody (mAb). .. We evaluated the use of gold nanoparticles (AuNPs) platform in a homogenous assay for a potency measurement of a therapeutic monoclonal antibody (mAb).

    Article Title: The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells
    Article Snippet: The Notch pathway signaling inhibitor DAPT (Sigma, D5942) was used at 5 μM. .. Construction of recombinant human DLL1 expression plasmid, protein production, and purification The cDNA fragment containing the human DLL1 signal peptide and sequences encoding the full extracellular domain was amplified by polymerase chain reaction from human DLL1 cDNA (Sino Biological, #HG11635-M, Beijing, China) using the primers: Fw: 5´- ATAGAATTCGCCGCCACCATGGGCAGTCGGTGCGCGCTGGC-3´ ; and Rev: 5´- GTCAGATCTGGATCCACGCGGAACCAGCCAGGGGAATGGCCCGCCCTG-3´ . .. The amplified cDNA was cloned into the mammalian pFUSE-IgG1-Fc vector (InvivoGen, pfuse-hg1fc1, Toulouse, France) in frame with Fc tag at the C-terminal region, using standard techniques.

    Expressing:

    Article Title: The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells
    Article Snippet: The Notch pathway signaling inhibitor DAPT (Sigma, D5942) was used at 5 μM. .. Construction of recombinant human DLL1 expression plasmid, protein production, and purification The cDNA fragment containing the human DLL1 signal peptide and sequences encoding the full extracellular domain was amplified by polymerase chain reaction from human DLL1 cDNA (Sino Biological, #HG11635-M, Beijing, China) using the primers: Fw: 5´- ATAGAATTCGCCGCCACCATGGGCAGTCGGTGCGCGCTGGC-3´ ; and Rev: 5´- GTCAGATCTGGATCCACGCGGAACCAGCCAGGGGAATGGCCCGCCCTG-3´ . .. The amplified cDNA was cloned into the mammalian pFUSE-IgG1-Fc vector (InvivoGen, pfuse-hg1fc1, Toulouse, France) in frame with Fc tag at the C-terminal region, using standard techniques.

    Plasmid Preparation:

    Article Title: The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells
    Article Snippet: The Notch pathway signaling inhibitor DAPT (Sigma, D5942) was used at 5 μM. .. Construction of recombinant human DLL1 expression plasmid, protein production, and purification The cDNA fragment containing the human DLL1 signal peptide and sequences encoding the full extracellular domain was amplified by polymerase chain reaction from human DLL1 cDNA (Sino Biological, #HG11635-M, Beijing, China) using the primers: Fw: 5´- ATAGAATTCGCCGCCACCATGGGCAGTCGGTGCGCGCTGGC-3´ ; and Rev: 5´- GTCAGATCTGGATCCACGCGGAACCAGCCAGGGGAATGGCCCGCCCTG-3´ . .. The amplified cDNA was cloned into the mammalian pFUSE-IgG1-Fc vector (InvivoGen, pfuse-hg1fc1, Toulouse, France) in frame with Fc tag at the C-terminal region, using standard techniques.

    Purification:

    Article Title: The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells
    Article Snippet: The Notch pathway signaling inhibitor DAPT (Sigma, D5942) was used at 5 μM. .. Construction of recombinant human DLL1 expression plasmid, protein production, and purification The cDNA fragment containing the human DLL1 signal peptide and sequences encoding the full extracellular domain was amplified by polymerase chain reaction from human DLL1 cDNA (Sino Biological, #HG11635-M, Beijing, China) using the primers: Fw: 5´- ATAGAATTCGCCGCCACCATGGGCAGTCGGTGCGCGCTGGC-3´ ; and Rev: 5´- GTCAGATCTGGATCCACGCGGAACCAGCCAGGGGAATGGCCCGCCCTG-3´ . .. The amplified cDNA was cloned into the mammalian pFUSE-IgG1-Fc vector (InvivoGen, pfuse-hg1fc1, Toulouse, France) in frame with Fc tag at the C-terminal region, using standard techniques.

    Amplification:

    Article Title: The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells
    Article Snippet: The Notch pathway signaling inhibitor DAPT (Sigma, D5942) was used at 5 μM. .. Construction of recombinant human DLL1 expression plasmid, protein production, and purification The cDNA fragment containing the human DLL1 signal peptide and sequences encoding the full extracellular domain was amplified by polymerase chain reaction from human DLL1 cDNA (Sino Biological, #HG11635-M, Beijing, China) using the primers: Fw: 5´- ATAGAATTCGCCGCCACCATGGGCAGTCGGTGCGCGCTGGC-3´ ; and Rev: 5´- GTCAGATCTGGATCCACGCGGAACCAGCCAGGGGAATGGCCCGCCCTG-3´ . .. The amplified cDNA was cloned into the mammalian pFUSE-IgG1-Fc vector (InvivoGen, pfuse-hg1fc1, Toulouse, France) in frame with Fc tag at the C-terminal region, using standard techniques.

    Polymerase Chain Reaction:

    Article Title: The Notch ligand DLL1 exerts carcinogenic features in human breast cancer cells
    Article Snippet: The Notch pathway signaling inhibitor DAPT (Sigma, D5942) was used at 5 μM. .. Construction of recombinant human DLL1 expression plasmid, protein production, and purification The cDNA fragment containing the human DLL1 signal peptide and sequences encoding the full extracellular domain was amplified by polymerase chain reaction from human DLL1 cDNA (Sino Biological, #HG11635-M, Beijing, China) using the primers: Fw: 5´- ATAGAATTCGCCGCCACCATGGGCAGTCGGTGCGCGCTGGC-3´ ; and Rev: 5´- GTCAGATCTGGATCCACGCGGAACCAGCCAGGGGAATGGCCCGCCCTG-3´ . .. The amplified cDNA was cloned into the mammalian pFUSE-IgG1-Fc vector (InvivoGen, pfuse-hg1fc1, Toulouse, France) in frame with Fc tag at the C-terminal region, using standard techniques.

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  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Sino Biological preparation human fcγriii protein
    (A) FPOP kinetic plots of representative residues of <t>FcγRIII.</t> The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.
    Preparation Human Fcγriii Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparation human fcγriii protein/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    preparation human fcγriii protein - by Bioz Stars, 2021-06
    92/100 stars
      Buy from Supplier

    Image Search Results


    (A) FPOP kinetic plots of representative residues of FcγRIII. The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.

    Journal: Biochemistry

    Article Title: Recognition of Human IgG1 by Fcγ Receptors: Structural Insights from Hydrogen-Deuterium Exchange and Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry

    doi: 10.1021/acs.biochem.8b01048

    Figure Lengend Snippet: (A) FPOP kinetic plots of representative residues of FcγRIII. The unbound and bound states of FcγIII receptor are shown in black and red, respectively. (B) FPOP kinetic plots of representative residues of IgG1 heavy chain (left) and light chain (right). The unbound and bound states of IgG1 are shown in blue and magenta, respectively. In each FPOP kinetic plot, the FPOP modified percentage is normalized by the modified percentage of reported peptide under each scavenging condition.

    Article Snippet: Materials and sample preparation Human FcγRIII protein was purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Modification

    (A) HDX kinetic plots of representative peptides of FcγRIII in the unbound (black) and bound (red) states. The peptide, its charge state, and its amino acid sequence are labeled on each panel. (B) Differential deuterium uptake percentage of FcγRIII mapped onto the crystal structure. The color scale representing the difference between bound and unbound is shown in the bottom of the panel. Mirrored views are provided for clarity.

    Journal: Biochemistry

    Article Title: Recognition of Human IgG1 by Fcγ Receptors: Structural Insights from Hydrogen-Deuterium Exchange and Fast Photochemical Oxidation of Proteins Coupled with Mass Spectrometry

    doi: 10.1021/acs.biochem.8b01048

    Figure Lengend Snippet: (A) HDX kinetic plots of representative peptides of FcγRIII in the unbound (black) and bound (red) states. The peptide, its charge state, and its amino acid sequence are labeled on each panel. (B) Differential deuterium uptake percentage of FcγRIII mapped onto the crystal structure. The color scale representing the difference between bound and unbound is shown in the bottom of the panel. Mirrored views are provided for clarity.

    Article Snippet: Materials and sample preparation Human FcγRIII protein was purchased from Sino Biological Inc. (Beijing, China).

    Techniques: Sequencing, Labeling