pre washed streptavidin agarose beads  (Millipore)


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    Structured Review

    Millipore pre washed streptavidin agarose beads
    Pre Washed Streptavidin Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre washed streptavidin agarose beads/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pre washed streptavidin agarose beads - by Bioz Stars, 2020-04
    91/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Hydrogen peroxide induces La cytoplasmic shuttling and increases hepatitis C virus internal ribosome entry site-dependent translation
    Article Snippet: Biotinylated RNA (10 µg) was mixed with 100 µg of S10 fraction in 500 µl binding buffer containing 10 mM Tris pH 7.4, 150 mM KCl, 1.5 mM MgCl2 , 0.5 mM DTT, 1 : 100 protease inhibitor cocktail (Sigma), 0.05 % NP40, 30 µg yeast tRNA (Sigma), 1 µl RNase inhibitor (New England Biolabs) for 30 min at room temperature followed by 2 h at 4 °C with slow rotation. .. To pulldown biotinylated RNA, 100 µl (1 : 1 slurry) of pre-washed streptavidin agarose beads (CL-4B, Sigma) were added to each reaction, and incubation continued at 4 °C for 2 h with slow rotation.

    Incubation:

    Article Title: Hydrogen peroxide induces La cytoplasmic shuttling and increases hepatitis C virus internal ribosome entry site-dependent translation
    Article Snippet: .. To pulldown biotinylated RNA, 100 µl (1 : 1 slurry) of pre-washed streptavidin agarose beads (CL-4B, Sigma) were added to each reaction, and incubation continued at 4 °C for 2 h with slow rotation. .. The beads were then washed five times with binding buffer and the pellets resuspended in 20 µl of SDS-PAGE loading buffer for electrophoretic separation on 12 % SDS-PAGE gel and Western blotting.

    Western Blot:

    Article Title: Hydrogen peroxide induces La cytoplasmic shuttling and increases hepatitis C virus internal ribosome entry site-dependent translation
    Article Snippet: To pulldown biotinylated RNA, 100 µl (1 : 1 slurry) of pre-washed streptavidin agarose beads (CL-4B, Sigma) were added to each reaction, and incubation continued at 4 °C for 2 h with slow rotation. .. The beads were then washed five times with binding buffer and the pellets resuspended in 20 µl of SDS-PAGE loading buffer for electrophoretic separation on 12 % SDS-PAGE gel and Western blotting.

    Binding Assay:

    Article Title: Hydrogen peroxide induces La cytoplasmic shuttling and increases hepatitis C virus internal ribosome entry site-dependent translation
    Article Snippet: Biotinylated RNA (10 µg) was mixed with 100 µg of S10 fraction in 500 µl binding buffer containing 10 mM Tris pH 7.4, 150 mM KCl, 1.5 mM MgCl2 , 0.5 mM DTT, 1 : 100 protease inhibitor cocktail (Sigma), 0.05 % NP40, 30 µg yeast tRNA (Sigma), 1 µl RNase inhibitor (New England Biolabs) for 30 min at room temperature followed by 2 h at 4 °C with slow rotation. .. To pulldown biotinylated RNA, 100 µl (1 : 1 slurry) of pre-washed streptavidin agarose beads (CL-4B, Sigma) were added to each reaction, and incubation continued at 4 °C for 2 h with slow rotation.

    SDS Page:

    Article Title: Hydrogen peroxide induces La cytoplasmic shuttling and increases hepatitis C virus internal ribosome entry site-dependent translation
    Article Snippet: To pulldown biotinylated RNA, 100 µl (1 : 1 slurry) of pre-washed streptavidin agarose beads (CL-4B, Sigma) were added to each reaction, and incubation continued at 4 °C for 2 h with slow rotation. .. The beads were then washed five times with binding buffer and the pellets resuspended in 20 µl of SDS-PAGE loading buffer for electrophoretic separation on 12 % SDS-PAGE gel and Western blotting.

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    Millipore streptavidin agarose beads
    Functional association of lytic granules with F-actin and effects of ATP and myosin IIA 1933x. Isolated lytic granules were added to flow chambers containing biotinylated <t>BSA/streptavidin</t> (BSA/SA) without actin, with actin and physiologic salt buffer,
    Streptavidin Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose beads/product/Millipore
    Average 97 stars, based on 293 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose beads - by Bioz Stars, 2020-04
    97/100 stars
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    Functional association of lytic granules with F-actin and effects of ATP and myosin IIA 1933x. Isolated lytic granules were added to flow chambers containing biotinylated BSA/streptavidin (BSA/SA) without actin, with actin and physiologic salt buffer,

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Myosin IIA associates with NK cell lytic granules to enable their interaction with F-actin and function at the immunological synapse 1

    doi: 10.4049/jimmunol.0804337

    Figure Lengend Snippet: Functional association of lytic granules with F-actin and effects of ATP and myosin IIA 1933x. Isolated lytic granules were added to flow chambers containing biotinylated BSA/streptavidin (BSA/SA) without actin, with actin and physiologic salt buffer,

    Article Snippet: Lysed granules were pre-cleared at 8200 g for 10 minutes, then incubated with streptavidin-agarose beads (Millipore) at 4°C for 1 hour.

    Techniques: Functional Assay, Isolation, Flow Cytometry