pre warmed 60°c tween 80 solution  (Millipore)


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    Name:
    TWEEN 80 solution
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    Catalog Number:
    p0343
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    Structured Review

    Millipore pre warmed 60°c tween 80 solution
    TWEEN 80 solution

    https://www.bioz.com/result/pre warmed 60°c tween 80 solution/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pre warmed 60°c tween 80 solution - by Bioz Stars, 2020-08
    94/100 stars

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    Injection:

    Article Title: Rapamycin is highly effective in murine models of immune-mediated bone marrow failure
    Article Snippet: .. Rapamycin was obtained from LC Laboratories (Woburn, MA, USA), dissolved in pure ethyl alcohol (The Warner-Graham Company, Cockeysville, MD, USA) at 50 mg/mL for storage at −30°C, diluted to 200 μg/mL with a 5% PEG-400 (polyethylene glycol MW 400, Sigma-Aldrich, St. Louis, MO, USA) and 5% Tween-80 (Sigma-Aldrich) solution before use, and administered through intraperitoneal injection (i.p.) once daily for 5–13 d at 2 mg/kg. .. CsA (NDC 0078-0109-01, Novartis Pharmaceutical Corporation, East Hanover, NJ, USA) was diluted in Iscove’s Modified Dulbecco’s Medium (IMDM) to 5 mg/mL and injected (i.p.) into animals at 50 mg/kg body weight once daily for 5–10 d. Both drugs were filtered through a 0.22 μM syringe-driven Millex-GS filter (Millipore Corporation, Billerica, MA, USA) before injection.

    other:

    Article Title: Toll-Like Receptor 7/8 (TLR7/8) and TLR9 Agonists Cooperate To Enhance HIV-1 Envelope Antibody Responses in Rhesus Macaques
    Article Snippet: The base adjuvant Span85-Tween 80-squalene (STS) was prepared by mixing Span85, Tween 80, and squalene (Sigma-Aldrich, St. Louis, MO; catalog numbers 85549, P8192, and 53626, respectively) at 0.5, 0.5, and 5% (vol/vol), respectively, in 1× phosphate-buffered saline (PBS; Gibco, Grand Island, NY) ( ).

    Article Title: Antiamoebic Activity of Adenophyllum aurantium (L.) Strother and Its Effect on the Actin Cytoskeleton of Entamoeba histolytica
    Article Snippet: Parasites Trophozoites of the HM1-IMSS E. histolytica strain and IP-1 Entamoeba invadens strain (ATCC 30994) were axenically cultivated in TYI-S-33 medium that was supplemented with 10% (v/v) adult bovine serum and 1% (v/v) Diamond Vitamin-Tween 80 solution (JRH Biosciences, Lenexa, KS, USA) at 37 and at 26°C, respectively ( ).

    Article Title: Structural and functional characterization of the divergent Entamoeba Src using Src inhibitor-1
    Article Snippet: Trophozoites of E. histolytica (HM1-IMSS) and E. invadens (IP-1) were cultivated in TYI-S-33 medium [ ] supplemented with 10% ( v / v ) bovine serum (BS) and 3% ( v / v ) Diamond vitamin-tween 80 solution (JRH Biosciences, Inc. St Louis, MO, USA) for 48 h in glass screw cap tubes (16 × 125 mm) at 37 °C.

    Article Title: Met Receptor Tyrosine Kinase and Chemoprevention of Oral Cancer
    Article Snippet: Crizotinib was dissolved in DMSO (P8192-5X10ML, Sigma Chemical, Sigma-Aldrich, Saint Louis, MO, USA).

    Article Title: Identification of N6,N6-Dimethyladenosine in Transfer RNA from Mycobacterium bovis Bacille Calmette-Guérin
    Article Snippet: 2’-O -Methyladenosine (Am), N4 -acetylcytidine (ac4 C), 5-methyluridine (m5 U), inosine (I), 2-methylguanosine (m2 G), N7 -methylguanosine (m7 G), 2’-O -methylcytidine (Cm), 3-methylcytidine (m3 C), 5-methylcytidine (m5 C), alkaline phosphatase, RNase A, ammonium acetate, geneticine, bovine serum albumin, deferoxamine mesylate, butylated hydroxytoluene, glucose, sodium chloride, nuclease P1, formic acid, and 20% Tween80 solution were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

    In Vitro:

    Article Title: Structural and functional characterization of the divergent Entamoeba Src using Src inhibitor-1
    Article Snippet: .. Parasite cultures and in vitro encystation Trophozoites of E. histolytica (HM1-IMSS) and E. invadens (IP-1) were cultivated in TYI-S-33 medium [ ] supplemented with 10% (v /v ) bovine serum (BS) and 3% (v /v ) Diamond vitamin-tween 80 solution (JRH Biosciences, Inc. St Louis, MO, USA) for 48 h in glass screw cap tubes (16 × 125 mm) at 37 °C. .. Trophozoites were subsequently incubated on ice for 10 min, collected by centrifugation at 1100 rpm for 10 min, and washed three times in TYI-S-33 medium without serum.

    Cell Culture:

    Article Title: Entamoeba histolytica Induce Signaling via Raf/MEK/ERK for Neutrophil Extracellular Trap (NET) Formation
    Article Snippet: .. Entamoeba histolytica trophozoites (strain HM1:IMSS) were cultured axenically at 37°C in TYIS-33 medium supplemented with 15% heat-inactivated adult bovine serum and Diamond vitamin Tween® 80 solution (Sigma-Aldrich; St. Louis, MO) (Diamond et al., ). ..

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  • 99
    Millipore mrc 5 cells
    (A) Percentage of viability, necrosis and early apoptosis in lung cancer and fibroblast cells determined by AnnexinV/7-AAD assay. (B) Cell cycle profile of <t>MRC-5</t> fibroblast cells, A-549, A-427, and MCF-7 tumor cells. ( 1 ) Normoxia, pH 7.2; ( 2 ) Normoxia, pH 6.2; ( 3 ) Hypoxia, pH 7.2 and ( 4 ) Hypoxia, pH 6.2. Bars represent mean+SEM. *p
    Mrc 5 Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mrc 5 cells/product/Millipore
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    mrc 5 cells - by Bioz Stars, 2020-08
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    91
    Millipore polysorbate 20
    Bacteriostatic and bactericidal activity of monocaprin-containing formulations. ( A – C ) Growth inhibition zones of all formulations. Sizes of zones of inhibited  N. gonorrhoeae  strain NCCP11945 growth represented in 2 mm rings of green around an 8 mm well with actual sizes in mm, as indicated. ( A ) Ointment formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( B ) CMC-based formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( C ) HPMC-based formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( D ) Log reduction of viable  N. gonorrhoeae  exposed for 2 min to HPMC-based formulations containing 0%, 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and 0%, 0.1%, 0.2%, 0.5%, 1%, and 2% polysorbate 20. Values over 4 log 10  are bactericidal (blue dashed line); those at 6 achieved total killing of all gonococci in the experiment (black dashed line).
    Polysorbate 20, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polysorbate 20/product/Millipore
    Average 91 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    polysorbate 20 - by Bioz Stars, 2020-08
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    Image Search Results


    (A) Percentage of viability, necrosis and early apoptosis in lung cancer and fibroblast cells determined by AnnexinV/7-AAD assay. (B) Cell cycle profile of MRC-5 fibroblast cells, A-549, A-427, and MCF-7 tumor cells. ( 1 ) Normoxia, pH 7.2; ( 2 ) Normoxia, pH 6.2; ( 3 ) Hypoxia, pH 7.2 and ( 4 ) Hypoxia, pH 6.2. Bars represent mean+SEM. *p

    Journal: Frontiers in Oncology

    Article Title: Lactic Acidosis in the Presence of Glucose Diminishes Warburg Effect in Lung Adenocarcinoma Cells

    doi: 10.3389/fonc.2020.00807

    Figure Lengend Snippet: (A) Percentage of viability, necrosis and early apoptosis in lung cancer and fibroblast cells determined by AnnexinV/7-AAD assay. (B) Cell cycle profile of MRC-5 fibroblast cells, A-549, A-427, and MCF-7 tumor cells. ( 1 ) Normoxia, pH 7.2; ( 2 ) Normoxia, pH 6.2; ( 3 ) Hypoxia, pH 7.2 and ( 4 ) Hypoxia, pH 6.2. Bars represent mean+SEM. *p

    Article Snippet: A-427, A-549 and MCF-7 cells were seeded at a density of 1 × 105 cells/mL, and 5 × 104 cells/mL were seeded for MRC-5 cells.

    Techniques:

    Glucose and lactate metabolism in tumor and fibroblast cells. (A) Kinetics of glucose and lactate levels from cultures of MRC-5, A-549, A-427, and MCF-7 followed through 96 h, using RPMI-1640 at pH 7.2 or pH 6.2 under normoxia (21% O 2 ) or hypoxia (2% O 2 ). (B) Specific rate of lactate production obtained during exponential phase of growth of tumor and fibroblast cells. (C) Specific rate of glucose consumption obtained during exponential phase of growth of tumor and fibroblast cells. Bars represent mean + SEM. * p

    Journal: Frontiers in Oncology

    Article Title: Lactic Acidosis in the Presence of Glucose Diminishes Warburg Effect in Lung Adenocarcinoma Cells

    doi: 10.3389/fonc.2020.00807

    Figure Lengend Snippet: Glucose and lactate metabolism in tumor and fibroblast cells. (A) Kinetics of glucose and lactate levels from cultures of MRC-5, A-549, A-427, and MCF-7 followed through 96 h, using RPMI-1640 at pH 7.2 or pH 6.2 under normoxia (21% O 2 ) or hypoxia (2% O 2 ). (B) Specific rate of lactate production obtained during exponential phase of growth of tumor and fibroblast cells. (C) Specific rate of glucose consumption obtained during exponential phase of growth of tumor and fibroblast cells. Bars represent mean + SEM. * p

    Article Snippet: A-427, A-549 and MCF-7 cells were seeded at a density of 1 × 105 cells/mL, and 5 × 104 cells/mL were seeded for MRC-5 cells.

    Techniques:

    Growth curves of MRC-5 fibroblast cells and A-549, A-427, and MCF-7 tumor cells followed through 96 h, using RPMI-1640 glucose (10 mM) and lactate (2 mM) adjusted at pH 7.2 or pH 6.2 under normoxia (21% O 2 ) or hypoxia (2% O 2 ).

    Journal: Frontiers in Oncology

    Article Title: Lactic Acidosis in the Presence of Glucose Diminishes Warburg Effect in Lung Adenocarcinoma Cells

    doi: 10.3389/fonc.2020.00807

    Figure Lengend Snippet: Growth curves of MRC-5 fibroblast cells and A-549, A-427, and MCF-7 tumor cells followed through 96 h, using RPMI-1640 glucose (10 mM) and lactate (2 mM) adjusted at pH 7.2 or pH 6.2 under normoxia (21% O 2 ) or hypoxia (2% O 2 ).

    Article Snippet: A-427, A-549 and MCF-7 cells were seeded at a density of 1 × 105 cells/mL, and 5 × 104 cells/mL were seeded for MRC-5 cells.

    Techniques:

    Left panel: representative flow cytometry profiles of AF83-7 aptamer at different concentrations in HT-29 and MRC-5 cells. Profiles are generated based on the data from six independent experiments. Right panel: binding of fluorescein-labeled AF83-7 and a scrambled negative control aptamer to HT-29 and MRC-5 cells under hypoxic conditions. Images are representative of three independent experiments.

    Journal: Nucleic Acids Research

    Article Title: Evoking picomolar binding in RNA by a single phosphorodithioate linkage

    doi: 10.1093/nar/gkw725

    Figure Lengend Snippet: Left panel: representative flow cytometry profiles of AF83-7 aptamer at different concentrations in HT-29 and MRC-5 cells. Profiles are generated based on the data from six independent experiments. Right panel: binding of fluorescein-labeled AF83-7 and a scrambled negative control aptamer to HT-29 and MRC-5 cells under hypoxic conditions. Images are representative of three independent experiments.

    Article Snippet: The HT-29 cells and MRC-5 cells were seeded in a 8-well slide (EMD Millipore, Billerica, MA, USA) in RPMI-1640 (Lonza, Basel, Switzerland)/10% FCS (Seradigm, Randor, PA, USA).

    Techniques: Flow Cytometry, Cytometry, Generated, Binding Assay, Labeling, Negative Control

    SIRT2 impact on protein levels of membrane trafficking proteins in human fibroblasts. A , SIRT2 mRNA levels upon shRNA-mediated knockdown in MRC5 cells. MRC5 cells stably expressing nontargeting shRNA (shCtrl) or shSIRT2 were subjected to mRNA extraction

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking *

    doi: 10.1074/mcp.M116.061333

    Figure Lengend Snippet: SIRT2 impact on protein levels of membrane trafficking proteins in human fibroblasts. A , SIRT2 mRNA levels upon shRNA-mediated knockdown in MRC5 cells. MRC5 cells stably expressing nontargeting shRNA (shCtrl) or shSIRT2 were subjected to mRNA extraction

    Article Snippet: Lentivirus-containing media was collected and used for transduction of MRC5 cells in the presence of 4 μg/ml polybrene (Millipore, Billerica, MA).

    Techniques: shRNA, Stable Transfection, Expressing

    SIRT2 interacts and colocalizes with liprin-β1 (PPFiBP1). A , SIRT2 colocalizes with PPFiBP1. SIRT2-EGFP MRC5 cells were imaged by direct fluorescence (SIRT2-EGFP, green) or immunofluorescence microscopy (anti-PPFiBP1, red). Bars - 25 μm.

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking *

    doi: 10.1074/mcp.M116.061333

    Figure Lengend Snippet: SIRT2 interacts and colocalizes with liprin-β1 (PPFiBP1). A , SIRT2 colocalizes with PPFiBP1. SIRT2-EGFP MRC5 cells were imaged by direct fluorescence (SIRT2-EGFP, green) or immunofluorescence microscopy (anti-PPFiBP1, red). Bars - 25 μm.

    Article Snippet: Lentivirus-containing media was collected and used for transduction of MRC5 cells in the presence of 4 μg/ml polybrene (Millipore, Billerica, MA).

    Techniques: Fluorescence, Immunofluorescence, Microscopy

    SIRT2 interaction network in human fibroblasts. A , Isolation efficiency of SIRT2-EGFP from IP with optimized conditions. SIRT2-EGFP IP was performed from MRC5 cells with optimized lysis buffer using anti-GFP antibody conjugated to magnetic beads via epoxy

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking *

    doi: 10.1074/mcp.M116.061333

    Figure Lengend Snippet: SIRT2 interaction network in human fibroblasts. A , Isolation efficiency of SIRT2-EGFP from IP with optimized conditions. SIRT2-EGFP IP was performed from MRC5 cells with optimized lysis buffer using anti-GFP antibody conjugated to magnetic beads via epoxy

    Article Snippet: Lentivirus-containing media was collected and used for transduction of MRC5 cells in the presence of 4 μg/ml polybrene (Millipore, Billerica, MA).

    Techniques: Isolation, Lysis, Magnetic Beads

    Experimental design for characterizing SIRT2 interactions in human fibroblasts. A , SIRT2-EGFP is localized to the cytoplasm. MRC5 cells stably expressing SIRT2-EGFP and EGFP were imaged by direct fluorescence microscopy, using DAPI as nuclear marker (blue).

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Human Sirtuin 2 Localization, Transient Interactions, and Impact on the Proteome Point to Its Role in Intracellular Trafficking *

    doi: 10.1074/mcp.M116.061333

    Figure Lengend Snippet: Experimental design for characterizing SIRT2 interactions in human fibroblasts. A , SIRT2-EGFP is localized to the cytoplasm. MRC5 cells stably expressing SIRT2-EGFP and EGFP were imaged by direct fluorescence microscopy, using DAPI as nuclear marker (blue).

    Article Snippet: Lentivirus-containing media was collected and used for transduction of MRC5 cells in the presence of 4 μg/ml polybrene (Millipore, Billerica, MA).

    Techniques: Stable Transfection, Expressing, Fluorescence, Microscopy, Marker

    Bacteriostatic and bactericidal activity of monocaprin-containing formulations. ( A – C ) Growth inhibition zones of all formulations. Sizes of zones of inhibited  N. gonorrhoeae  strain NCCP11945 growth represented in 2 mm rings of green around an 8 mm well with actual sizes in mm, as indicated. ( A ) Ointment formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( B ) CMC-based formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( C ) HPMC-based formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( D ) Log reduction of viable  N. gonorrhoeae  exposed for 2 min to HPMC-based formulations containing 0%, 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and 0%, 0.1%, 0.2%, 0.5%, 1%, and 2% polysorbate 20. Values over 4 log 10  are bactericidal (blue dashed line); those at 6 achieved total killing of all gonococci in the experiment (black dashed line).

    Journal: Scientific Reports

    Article Title: Monocaprin eye drop formulation to combat antibiotic resistant gonococcal blindness

    doi: 10.1038/s41598-020-68722-8

    Figure Lengend Snippet: Bacteriostatic and bactericidal activity of monocaprin-containing formulations. ( A – C ) Growth inhibition zones of all formulations. Sizes of zones of inhibited N. gonorrhoeae strain NCCP11945 growth represented in 2 mm rings of green around an 8 mm well with actual sizes in mm, as indicated. ( A ) Ointment formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( B ) CMC-based formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( C ) HPMC-based formulations with 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and a no monocaprin control. ( D ) Log reduction of viable N. gonorrhoeae exposed for 2 min to HPMC-based formulations containing 0%, 0.062%, 0.125%, 0.188%, and 0.25% monocaprin and 0%, 0.1%, 0.2%, 0.5%, 1%, and 2% polysorbate 20. Values over 4 log 10 are bactericidal (blue dashed line); those at 6 achieved total killing of all gonococci in the experiment (black dashed line).

    Article Snippet: The polymer was then placed at 4 °C for an hour and then added to the double concentrate of polysorbate 20, monocaprin, and propan-1,2-diol as for the CMC-based formulations.

    Techniques: Activity Assay, Inhibition

    Comparison of the influence of polymer and polysorbate 20 on the solubility of monocaprin in thickened eye drop formulations. In CMC-based formulations, monocaprin forms clumps (indicated by a red asterisk) at 0.125% and above. Addition of 0.1% polysorbate 20 evenly distributed the monocaprin, although it did not fully solubilise. In HPMC-based formulations, monocaprin forms a fine precipitate just under the meniscus at 0.125% and above (indicated by a red hash). Addition of 0.1% polysorbate 20 solubilised the monocaprin.

    Journal: Scientific Reports

    Article Title: Monocaprin eye drop formulation to combat antibiotic resistant gonococcal blindness

    doi: 10.1038/s41598-020-68722-8

    Figure Lengend Snippet: Comparison of the influence of polymer and polysorbate 20 on the solubility of monocaprin in thickened eye drop formulations. In CMC-based formulations, monocaprin forms clumps (indicated by a red asterisk) at 0.125% and above. Addition of 0.1% polysorbate 20 evenly distributed the monocaprin, although it did not fully solubilise. In HPMC-based formulations, monocaprin forms a fine precipitate just under the meniscus at 0.125% and above (indicated by a red hash). Addition of 0.1% polysorbate 20 solubilised the monocaprin.

    Article Snippet: The polymer was then placed at 4 °C for an hour and then added to the double concentrate of polysorbate 20, monocaprin, and propan-1,2-diol as for the CMC-based formulations.

    Techniques: Solubility