pre blocked wet streptavidin agarose beads  (Millipore)


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    Structured Review

    Millipore pre blocked wet streptavidin agarose beads
    Pre Blocked Wet Streptavidin Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pre blocked wet streptavidin agarose beads/product/Millipore
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pre blocked wet streptavidin agarose beads - by Bioz Stars, 2020-04
    91/100 stars

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    nuclear-enriched protein lysate

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    Western Blot:

    Article Title: Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation
    Article Snippet: One mg of nuclear-enriched protein lysate was incubated with 100μL pre-blocked wet streptavidin agarose beads (Novagen). .. After the final wash, equal amount of 2X Laemmli sample buffer was added to the dried beads and boiled for 10min to elute the EdU-bound fraction for western blot analysis.

    Incubation:

    Article Title: Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation
    Article Snippet: .. One mg of nuclear-enriched protein lysate was incubated with 100μL pre-blocked wet streptavidin agarose beads (Novagen). ..

    Labeling:

    Article Title: Chronic DNA Replication Stress Reduces Replicative Lifespan of Cells by TRP53-Dependent, microRNA-Assisted MCM2-7 Downregulation
    Article Snippet: EdU pulse labeling was performed for 30 min. for all experiments. .. One mg of nuclear-enriched protein lysate was incubated with 100μL pre-blocked wet streptavidin agarose beads (Novagen).

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    Millipore bovine serum albumin bsa
    Bovine Serum Albumin Bsa, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore biotinylated heparin
    Recombinant proteins F1 683 –F5 683 bind heparin. A , binding of recombinant protein to <t>biotinylated</t> heparin. Total binding (■) was determined by coating recombinant protein F1 683 –F5 683 (10 μg ml −1 ) to 96-well microtiter
    Biotinylated Heparin, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated heparin/product/Millipore
    Average 93 stars, based on 14 article reviews
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    biotinylated heparin - by Bioz Stars, 2020-04
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    97
    Millipore streptavidin agarose beads
    Functional association of lytic granules with F-actin and effects of ATP and myosin IIA 1933x. Isolated lytic granules were added to flow chambers containing biotinylated <t>BSA/streptavidin</t> (BSA/SA) without actin, with actin and physiologic salt buffer,
    Streptavidin Agarose Beads, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 293 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin agarose beads/product/Millipore
    Average 97 stars, based on 293 article reviews
    Price from $9.99 to $1999.99
    streptavidin agarose beads - by Bioz Stars, 2020-04
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    Recombinant proteins F1 683 –F5 683 bind heparin. A , binding of recombinant protein to biotinylated heparin. Total binding (■) was determined by coating recombinant protein F1 683 –F5 683 (10 μg ml −1 ) to 96-well microtiter

    Journal: The Journal of Biological Chemistry

    Article Title: Sequence TTKF?QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma hyopneumoniae *

    doi: 10.1074/jbc.M111.226084

    Figure Lengend Snippet: Recombinant proteins F1 683 –F5 683 bind heparin. A , binding of recombinant protein to biotinylated heparin. Total binding (■) was determined by coating recombinant protein F1 683 –F5 683 (10 μg ml −1 ) to 96-well microtiter

    Article Snippet: Pre-diluted biotinylated heparin (Calbiochem) was added to wells in serial 2-fold dilutions starting from 100 μg ml−1 , and plates were incubated at room temperature for 1 h with shaking.

    Techniques: Recombinant, Binding Assay

    Functional association of lytic granules with F-actin and effects of ATP and myosin IIA 1933x. Isolated lytic granules were added to flow chambers containing biotinylated BSA/streptavidin (BSA/SA) without actin, with actin and physiologic salt buffer,

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Myosin IIA associates with NK cell lytic granules to enable their interaction with F-actin and function at the immunological synapse 1

    doi: 10.4049/jimmunol.0804337

    Figure Lengend Snippet: Functional association of lytic granules with F-actin and effects of ATP and myosin IIA 1933x. Isolated lytic granules were added to flow chambers containing biotinylated BSA/streptavidin (BSA/SA) without actin, with actin and physiologic salt buffer,

    Article Snippet: Lysed granules were pre-cleared at 8200 g for 10 minutes, then incubated with streptavidin-agarose beads (Millipore) at 4°C for 1 hour.

    Techniques: Functional Assay, Isolation, Flow Cytometry