recombinant myristoylated hiv 1 nef protein rnef  (Jena Bioscience)


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    Name:
    HIV 1 Nef
    Description:

    Catalog Number:
    PR-1214
    Price:
    217.3
    Category:
    Proteins
    Size:
    100 µg
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    Structured Review

    Jena Bioscience recombinant myristoylated hiv 1 nef protein rnef
    <t>HIV-1</t> Nef inhibits translation in vitro . The level of translation of the luciferase mRNA has been measured in the presence of increasing concentrations of <t>rNef,</t> rVpr or recombinant ovalbumin in RRL. Recombinant HIV-1 Vpr and ovalbumin were used to test the specificity of Nef action on translation. In addition an inactive form of Nef was obtained by heating at 100°C for 5 min (HI).

    https://www.bioz.com/result/recombinant myristoylated hiv 1 nef protein rnef/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant myristoylated hiv 1 nef protein rnef - by Bioz Stars, 2021-06
    85/100 stars

    Images

    1) Product Images from "The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA"

    Article Title: The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA

    Journal: Virology Journal

    doi: 10.1186/1743-422X-9-103

    HIV-1 Nef inhibits translation in vitro . The level of translation of the luciferase mRNA has been measured in the presence of increasing concentrations of rNef, rVpr or recombinant ovalbumin in RRL. Recombinant HIV-1 Vpr and ovalbumin were used to test the specificity of Nef action on translation. In addition an inactive form of Nef was obtained by heating at 100°C for 5 min (HI).
    Figure Legend Snippet: HIV-1 Nef inhibits translation in vitro . The level of translation of the luciferase mRNA has been measured in the presence of increasing concentrations of rNef, rVpr or recombinant ovalbumin in RRL. Recombinant HIV-1 Vpr and ovalbumin were used to test the specificity of Nef action on translation. In addition an inactive form of Nef was obtained by heating at 100°C for 5 min (HI).

    Techniques Used: In Vitro, Luciferase, Recombinant

    Related Articles

    Produced:

    Article Title: HIV-1 proteins preferentially activate anti-inflammatory M2-type macrophages.
    Article Snippet: The gp120 protein (LAV strain) produced in insect cells was purchased from Protein Sciences (Meriden, CT). .. The C-terminal His-tagged 210-amino acid HIV-1 Nef proteins (SF2 strain) produced in E. coli were purchased from Jena Bioscience (Jena, Germany). .. The preparation was shown to be free of endotoxin (,0.03 U/ml), as determined by a Limulus assay (33, 34).

    Article Title: Macropinocytosis and TAK1 mediate anti-inflammatory to pro-inflammatory macrophage differentiation by HIV-1 Nef
    Article Snippet: .. Recombinant Nef proteins and cytokines C-terminally His-tagged 210-amino acid HIV-1 Nef proteins (SF2 strain) produced in E. coli were purchased from Jena Bioscience (Jena, Germany). ..

    Recombinant:

    Article Title: Macropinocytosis and TAK1 mediate anti-inflammatory to pro-inflammatory macrophage differentiation by HIV-1 Nef
    Article Snippet: .. Recombinant Nef proteins and cytokines C-terminally His-tagged 210-amino acid HIV-1 Nef proteins (SF2 strain) produced in E. coli were purchased from Jena Bioscience (Jena, Germany). ..

    Article Title: The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA
    Article Snippet: Peripheral blood mononuclear cells (PBMCs), primary monocyte-derived macrophages (MDMs) and peripheral blood lymphocytes (PBLs) were prepared from peripheral blood of healthy donors and were cultured in RPMI medium supplemented with 10% (v/v) pooled AB human serum (Sigma, Munich, Germany), as previously described [ ]. .. Recombinant Nef treatment Cells (5x106 cells) were treated with recombinant myristoylated HIV-1 Nef protein (rNef) (SF2 strain) from Jena Bioscience (cat # PR-382). .. Cell pellets were collected at various periods of time after treatment with rNef, washed extensively and lyzed before western-blot analysis.

    Article Title: Human and simian immunodeficiency viruses deregulate early hematopoiesis through a Nef/PPAR?/STAT5 signaling pathway in macaques
    Article Snippet: Recombinant HIV-1 Laï Nef and SIVmac251 Nef antibody (ARP3201) were provided by the National Institute for Biological Standards and Control (NIBSC) Centralized Facility for AIDS Reagents (repository reference, EVA650) or CEA/DIEP. .. Recombinant myristoylated HIV-1 Nef from strain SF2 (Jena Bioscience) was generated as reported ( ). .. Nef-derived peptides (15AA) were synthesized using standard Fmoc chemistry; peptide B and mutated peptide B were produced by NeoMPS.

    Article Title: Human immunodeficiency virus 1 Nef suppresses CD40-dependent immunoglobulin class switching in bystander B cells.
    Article Snippet: Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines. .. Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines. .. Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines.

    Article Title: Nef Inhibits Glucose Uptake in Adipocytes and Contributes to Insulin Resistance in Human Immunodeficiency Virus Type I Infection
    Article Snippet: .. Adipocytes were inoculated onto 24-well culture plates and treated overnight with 0.005, 0.01, 0.05, and 0.1 μg/mL recombinant myristoylated HIV-1 Nef. .. Cells were serum-starved, washed twice with Krebs–Ringer bicarbonate buffer containing 30 mM HEPES, pH 7.4, and 0.1% bovine seroalbumin (BSA) (Krebs–Ringer bicarbonate/HEPES [KRBH]/BSA) and incubated in KRBH/BSA for 30 min. Two wells per treatment were incubated in KRBH/BSA/0.4 mM Cytochalasin B (Sigma-Aldrich) to determine nonspecific glucose uptake.

    Article Title: Nef Inhibits Glucose Uptake in Adipocytes and Contributes to Insulin Resistance in Human Immunodeficiency Virus Type I Infection
    Article Snippet: .. Before experiments, cells were treated overnight with 0.1 μg/mL recombinant myristoylated HIV-1 Nef (Jena Biosciences) or nonmyristoylated Nef (National Institutes of Health AIDS Reference and Reagent Repository) unless noted otherwise. ..

    Generated:

    Article Title: Human and simian immunodeficiency viruses deregulate early hematopoiesis through a Nef/PPAR?/STAT5 signaling pathway in macaques
    Article Snippet: Recombinant HIV-1 Laï Nef and SIVmac251 Nef antibody (ARP3201) were provided by the National Institute for Biological Standards and Control (NIBSC) Centralized Facility for AIDS Reagents (repository reference, EVA650) or CEA/DIEP. .. Recombinant myristoylated HIV-1 Nef from strain SF2 (Jena Bioscience) was generated as reported ( ). .. Nef-derived peptides (15AA) were synthesized using standard Fmoc chemistry; peptide B and mutated peptide B were produced by NeoMPS.

    Article Title: Human immunodeficiency virus 1 Nef suppresses CD40-dependent immunoglobulin class switching in bystander B cells.
    Article Snippet: Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines. .. Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines. .. Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines.

    Concentration Assay:

    Article Title: Human immunodeficiency virus 1 Nef suppresses CD40-dependent immunoglobulin class switching in bystander B cells.
    Article Snippet: Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines. .. Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines. .. Immunoglobulin class switching from immunoglobulin M (IgM) to IgG and IgA is central to immunity against viruses and requires the activation of B cells by T cells via CD154 (CD40 ligand) and cytokines.

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  • 85
    Jena Bioscience recombinant myristoylated hiv 1 nef protein rnef
    <t>HIV-1</t> Nef inhibits translation in vitro . The level of translation of the luciferase mRNA has been measured in the presence of increasing concentrations of <t>rNef,</t> rVpr or recombinant ovalbumin in RRL. Recombinant HIV-1 Vpr and ovalbumin were used to test the specificity of Nef action on translation. In addition an inactive form of Nef was obtained by heating at 100°C for 5 min (HI).
    Recombinant Myristoylated Hiv 1 Nef Protein Rnef, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant myristoylated hiv 1 nef protein rnef/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant myristoylated hiv 1 nef protein rnef - by Bioz Stars, 2021-06
    85/100 stars
      Buy from Supplier

    Image Search Results


    HIV-1 Nef inhibits translation in vitro . The level of translation of the luciferase mRNA has been measured in the presence of increasing concentrations of rNef, rVpr or recombinant ovalbumin in RRL. Recombinant HIV-1 Vpr and ovalbumin were used to test the specificity of Nef action on translation. In addition an inactive form of Nef was obtained by heating at 100°C for 5 min (HI).

    Journal: Virology Journal

    Article Title: The HIV-1 Nef Protein Interacts with two components of the 40S small ribosomal subunit, the RPS10 protein and the 18S rRNA

    doi: 10.1186/1743-422X-9-103

    Figure Lengend Snippet: HIV-1 Nef inhibits translation in vitro . The level of translation of the luciferase mRNA has been measured in the presence of increasing concentrations of rNef, rVpr or recombinant ovalbumin in RRL. Recombinant HIV-1 Vpr and ovalbumin were used to test the specificity of Nef action on translation. In addition an inactive form of Nef was obtained by heating at 100°C for 5 min (HI).

    Article Snippet: Recombinant Nef treatment Cells (5x106 cells) were treated with recombinant myristoylated HIV-1 Nef protein (rNef) (SF2 strain) from Jena Bioscience (cat # PR-382).

    Techniques: In Vitro, Luciferase, Recombinant