pqe30 expression vector  (Qiagen)

 
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    Name:
    pQE 30 Xa Vector
    Description:
    For expression of His tagged proteins containing a Factor Xa Protease recognition site Kit contents Qiagen pQE 30 Xa Vector DNA 25μg N terminal Tag 6xHis Tag Factor Xa Recognition Site In vivo Expression For Expression of His tagged Proteins Containing a Factor Xa Protease Recognition Site Ideal for Protein Crystallography Structure Determination Studies using NMR Benefits Easy cloning of a construct with a Factor Xa Protease recognition si
    Catalog Number:
    33203
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    pQE 30 Xa Vector
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    Structured Review

    Qiagen pqe30 expression vector
    pQE 30 Xa Vector
    For expression of His tagged proteins containing a Factor Xa Protease recognition site Kit contents Qiagen pQE 30 Xa Vector DNA 25μg N terminal Tag 6xHis Tag Factor Xa Recognition Site In vivo Expression For Expression of His tagged Proteins Containing a Factor Xa Protease Recognition Site Ideal for Protein Crystallography Structure Determination Studies using NMR Benefits Easy cloning of a construct with a Factor Xa Protease recognition si
    https://www.bioz.com/result/pqe30 expression vector/product/Qiagen
    Average 99 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    pqe30 expression vector - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "The N-Terminal Region of Arabidopsis Cystathionine ?-Synthase Plays an Important Regulatory Role in Methionine Metabolism 1"

    Article Title: The N-Terminal Region of Arabidopsis Cystathionine ?-Synthase Plays an Important Regulatory Role in Methionine Metabolism 1

    Journal: Plant Physiology

    doi: 10.1104/pp.010819

    Functional complementation of CGS-deficient E. coli mutant LE392 with Arabidopsis CGS cDNAs. The plasmid pQE30 (as control), the same plasmid containing the full-length Arabidopsis CGS, and the plasmid containing the truncated version (without the N-terminal region) of CGS were transformed into the E. coli mutant. The transformed bacteria were plated onto an M9 minimal medium with (right) or without (left) Met (40 μg mL −1 ). Plates were incubated at 37°C for 36 h.
    Figure Legend Snippet: Functional complementation of CGS-deficient E. coli mutant LE392 with Arabidopsis CGS cDNAs. The plasmid pQE30 (as control), the same plasmid containing the full-length Arabidopsis CGS, and the plasmid containing the truncated version (without the N-terminal region) of CGS were transformed into the E. coli mutant. The transformed bacteria were plated onto an M9 minimal medium with (right) or without (left) Met (40 μg mL −1 ). Plates were incubated at 37°C for 36 h.

    Techniques Used: Functional Assay, Mutagenesis, Plasmid Preparation, Transformation Assay, Incubation

    2) Product Images from "Plasmodium falciparum Hep1 Is Required to Prevent the Self Aggregation of PfHsp70-3"

    Article Title: Plasmodium falciparum Hep1 Is Required to Prevent the Self Aggregation of PfHsp70-3

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0156446

    PfHep1 enhanced the solubility of PfHsp70-3 and facilitated native purification. ( A ) SDS-PAGE (10%) analysis of the solubility of PfHsp70-3 in the presence and absence of PfHep1. Lane M: protein markers in kDa, lane 1: E . coli BL21(DE3) [pQE30-PfHsp70-3] 4 hrs post IPTG induction (total protein), lanes 2–3: supernatant and pellet fractions of cells harvested and lysed 4 hrs post IPTG induction, lane 4: E . coli BL21(DE3) [pQE30-PfHsp70-3; pACYCDuet1-PfHep1] 4 hrs post IPTG induction (total protein), lanes 5–6: supernatant and pellet fractions from lysed cells co-transformed with pQE30-PfHsp70-3 and pACYCDuet-1-PfHep1 4 hrs post IPTG induction. ( B ) Western analysis using anti-His antibody. ( C ) SDS-PAGE (10%) analysis of the purification of PfHsp70-3, after co-expression with PfHep1, by nickel affinity chromatography. Lane M: protein markers in kDa, lane 1: E . coli BL21 (DE3) [pQE30-PfHsp70-3; pACYCDuet1-PfHep1] 4hrs post IPTG induction, lane 2: fraction unbound to the cOmplete His-tag purification resin, lanes 3–5: washes containing 50 mM imidazole, lanes 6–8: elutions of PfHsp70-3 and PfHep1 using 750 mM imidazole, lane 9: bead fraction. ( D ) Western analysis for detection of PfHsp70-3 (top) and PfHep1 (bottom) using anti-His antibodies.
    Figure Legend Snippet: PfHep1 enhanced the solubility of PfHsp70-3 and facilitated native purification. ( A ) SDS-PAGE (10%) analysis of the solubility of PfHsp70-3 in the presence and absence of PfHep1. Lane M: protein markers in kDa, lane 1: E . coli BL21(DE3) [pQE30-PfHsp70-3] 4 hrs post IPTG induction (total protein), lanes 2–3: supernatant and pellet fractions of cells harvested and lysed 4 hrs post IPTG induction, lane 4: E . coli BL21(DE3) [pQE30-PfHsp70-3; pACYCDuet1-PfHep1] 4 hrs post IPTG induction (total protein), lanes 5–6: supernatant and pellet fractions from lysed cells co-transformed with pQE30-PfHsp70-3 and pACYCDuet-1-PfHep1 4 hrs post IPTG induction. ( B ) Western analysis using anti-His antibody. ( C ) SDS-PAGE (10%) analysis of the purification of PfHsp70-3, after co-expression with PfHep1, by nickel affinity chromatography. Lane M: protein markers in kDa, lane 1: E . coli BL21 (DE3) [pQE30-PfHsp70-3; pACYCDuet1-PfHep1] 4hrs post IPTG induction, lane 2: fraction unbound to the cOmplete His-tag purification resin, lanes 3–5: washes containing 50 mM imidazole, lanes 6–8: elutions of PfHsp70-3 and PfHep1 using 750 mM imidazole, lane 9: bead fraction. ( D ) Western analysis for detection of PfHsp70-3 (top) and PfHep1 (bottom) using anti-His antibodies.

    Techniques Used: Solubility, Purification, SDS Page, Transformation Assay, Western Blot, Expressing, Affinity Chromatography

    Solubilization and purification of PfHep1. ( A ) SDS-PAGE (10%) analysis of the solubility of PfHep1 before and after addition of sarcosyl. Lane M: protein markers in kDa, lanes 1–2: supernatant and pellet fractions of cells not treated with sarcosyl, lanes 3–4: supernatant and pellet fraction of cells treated with 3% sarcosyl. ( B ) Western analysis using anti-His antibody. ( C ) Purification of PfHep1 after solubilization with 3% sarcosyl, lane M: protein markers in kDa, lane 1: E . coli M15 ([pREP4]; pQE30-PfHep1) 4 hrs post IPTG induction, lane 2: fraction unbound to the cOmplete His-tag purification resin, lanes 3–5: washes using 50 mM imidazole, lanes 6–8: elutions of PfHep1 using 750 mM imidazole, and lane 9: bead fraction. ( D ) Western analysis for detection of PfHep1 using anti-His antibodies.
    Figure Legend Snippet: Solubilization and purification of PfHep1. ( A ) SDS-PAGE (10%) analysis of the solubility of PfHep1 before and after addition of sarcosyl. Lane M: protein markers in kDa, lanes 1–2: supernatant and pellet fractions of cells not treated with sarcosyl, lanes 3–4: supernatant and pellet fraction of cells treated with 3% sarcosyl. ( B ) Western analysis using anti-His antibody. ( C ) Purification of PfHep1 after solubilization with 3% sarcosyl, lane M: protein markers in kDa, lane 1: E . coli M15 ([pREP4]; pQE30-PfHep1) 4 hrs post IPTG induction, lane 2: fraction unbound to the cOmplete His-tag purification resin, lanes 3–5: washes using 50 mM imidazole, lanes 6–8: elutions of PfHep1 using 750 mM imidazole, and lane 9: bead fraction. ( D ) Western analysis for detection of PfHep1 using anti-His antibodies.

    Techniques Used: Purification, SDS Page, Solubility, Western Blot

    3) Product Images from "Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus"

    Article Title: Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus

    Journal: Journal of Virological Methods

    doi: 10.1016/S0166-0934(03)00064-8

    SDS-PAGE analysis of the expression of the rM protein in E. coli . Lanes 1 and 2: crude material from the bacterial cultures containing the pQE30 expression vectors with the cDNA insert encoding the M protein, before (lane 1) and after (lane 2) the induction. Lane 3: purified electroeluted rM protein. M, markers (phosphorylase b, serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor and lysozyme).
    Figure Legend Snippet: SDS-PAGE analysis of the expression of the rM protein in E. coli . Lanes 1 and 2: crude material from the bacterial cultures containing the pQE30 expression vectors with the cDNA insert encoding the M protein, before (lane 1) and after (lane 2) the induction. Lane 3: purified electroeluted rM protein. M, markers (phosphorylase b, serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor and lysozyme).

    Techniques Used: SDS Page, Expressing, Purification

    4) Product Images from "Recombinant 56-Kilodalton Major Outer Membrane Protein Antigen of Orientia tsutsugamushi Shanxi and Its Antigenicity "

    Article Title: Recombinant 56-Kilodalton Major Outer Membrane Protein Antigen of Orientia tsutsugamushi Shanxi and Its Antigenicity

    Journal: Infection and Immunity

    doi: 10.1128/IAI.71.8.4772-4779.2003

    SDS-10% PAGE analysis of the expression products of E. coli M15 harboring pQE30/ sxh56 . Lane M, protein molecular weight marker; lanes 1 to 3, E. coli M15 harboring pQE30/ sxh 56 induced by IPTG; lane 4, E. coli M15 harboring pQE30 induced by IPTG; lane 5, E. coli M15 induced by IPTG; lane 6, protein expressed as inclusion bodies; lane 7, supernatant of supersonic crash.
    Figure Legend Snippet: SDS-10% PAGE analysis of the expression products of E. coli M15 harboring pQE30/ sxh56 . Lane M, protein molecular weight marker; lanes 1 to 3, E. coli M15 harboring pQE30/ sxh 56 induced by IPTG; lane 4, E. coli M15 harboring pQE30 induced by IPTG; lane 5, E. coli M15 induced by IPTG; lane 6, protein expressed as inclusion bodies; lane 7, supernatant of supersonic crash.

    Techniques Used: Polyacrylamide Gel Electrophoresis, Expressing, Molecular Weight, Marker

    Strategy for cloning and construction of pQE30/56, which expresses the recombinant 56-kDa protein of the O. tsutsugamushi Shanxi strain.
    Figure Legend Snippet: Strategy for cloning and construction of pQE30/56, which expresses the recombinant 56-kDa protein of the O. tsutsugamushi Shanxi strain.

    Techniques Used: Clone Assay, Recombinant

    Identification of the recombinant plasmid by digestion with Bam HI plus Hin dIII and PCR. Lane M, λ DNA/EcoT14I standard marker; lane 1, recombinant plasmid pQE30/56 digested with Bam HI plus Hin dIII; lane 2, pQE30 digested with Bam HI plus Hin dIII; lane 3, PCR product of recombinant plasmid pQE30/56.
    Figure Legend Snippet: Identification of the recombinant plasmid by digestion with Bam HI plus Hin dIII and PCR. Lane M, λ DNA/EcoT14I standard marker; lane 1, recombinant plasmid pQE30/56 digested with Bam HI plus Hin dIII; lane 2, pQE30 digested with Bam HI plus Hin dIII; lane 3, PCR product of recombinant plasmid pQE30/56.

    Techniques Used: Recombinant, Plasmid Preparation, Polymerase Chain Reaction, Marker

    Immunoblot analysis of the recombinant Sxh56 protein produced by the pQE30/ sxh 56 clone. Crude cell extracts were obtained from E. coli cells containing recombinant pQE30/ sxh 56 plasmids, separated by SDS-10% PAGE, transferred to nitrocellulose membranes, and reacted with anti- O. tsutsugamushi Sxh951 antibodies (see Materials and Methods). Molecular masses are indicated on the left. Lane M, protein molecular weight marker; lane 1, E. coli M15 harboring pQE30/ sxh 56 induced by IPTG; lane 2, E. coli M15 harboring pQE30 induced by IPTG; lane 3, E. coli M15 induced by IPTG.
    Figure Legend Snippet: Immunoblot analysis of the recombinant Sxh56 protein produced by the pQE30/ sxh 56 clone. Crude cell extracts were obtained from E. coli cells containing recombinant pQE30/ sxh 56 plasmids, separated by SDS-10% PAGE, transferred to nitrocellulose membranes, and reacted with anti- O. tsutsugamushi Sxh951 antibodies (see Materials and Methods). Molecular masses are indicated on the left. Lane M, protein molecular weight marker; lane 1, E. coli M15 harboring pQE30/ sxh 56 induced by IPTG; lane 2, E. coli M15 harboring pQE30 induced by IPTG; lane 3, E. coli M15 induced by IPTG.

    Techniques Used: Recombinant, Produced, Polyacrylamide Gel Electrophoresis, Molecular Weight, Marker

    5) Product Images from "Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus"

    Article Title: Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus

    Journal: Journal of Virological Methods

    doi: 10.1016/S0166-0934(03)00064-8

    SDS-PAGE analysis of the expression of the rM protein in E. coli . Lanes 1 and 2: crude material from the bacterial cultures containing the pQE30 expression vectors with the cDNA insert encoding the M protein, before (lane 1) and after (lane 2) the induction. Lane 3: purified electroeluted rM protein. M, markers (phosphorylase b, serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor and lysozyme).
    Figure Legend Snippet: SDS-PAGE analysis of the expression of the rM protein in E. coli . Lanes 1 and 2: crude material from the bacterial cultures containing the pQE30 expression vectors with the cDNA insert encoding the M protein, before (lane 1) and after (lane 2) the induction. Lane 3: purified electroeluted rM protein. M, markers (phosphorylase b, serum albumin, ovalbumin, carbonic anhydrase, trypsin inhibitor and lysozyme).

    Techniques Used: SDS Page, Expressing, Purification

    Related Articles

    Clone Assay:

    Article Title: A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus
    Article Snippet: .. Construction of expression plasmids For the expression of HCV proteins in E. coli , corresponding coding sequences were cloned into the histidine fusion expression vectors, pET32a(+) and pQE30, or the GST-tag expression vector, pGEX-4T-2, as shown in and . .. Recombinant plasmids were examined and confirmed by PCR amplification, restriction enzyme digestion and DNA sequencing.

    Article Title: Monoclonal Antibodies against the Fusion Peptide of Hemagglutinin Protect Mice from Lethal Influenza A Virus H5N1 Infection ▿
    Article Snippet: .. The HA gene and the HA2 gene were amplified from the cDNA and cloned into pQE-30 vector (Qiagen, Germany), using standard cloning techniques for expression in bacteria. .. The clones were transformed into Escherichia coli M15/pREP4 competent cells for protein expression.

    Article Title: A thermostable endonuclease III homolog from the archaeon Pyrobaculum aerophilum
    Article Snippet: .. The PCR product was cloned into pCR2.1-TOPO vector using TOPO TA Cloning kit (Invitrogen, Carlsbad, CA) and subsequently subcloned into the bacterial expression vector pQE30 (Qiagen, Chatsworth, CA) between the Bam HI and Hin dIII sites. ..

    Article Title: Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis
    Article Snippet: .. Expression vector pQE30 (Qiagen) contains the T5 promoter for high-level expression, a ribosome binding site, and × histidine coding sequences followed by a multiple cloning site. .. The vector also contains two lac operator sequences.

    Article Title: Unique Epitope of Bovine Immunodeficiency Virus Gag Protein Spans the Cleavage Site between p16MA and p2L †
    Article Snippet: .. For cloning of the deduced epitope into the pQE30Xa expression vector (QIAGEN), the forward primer had an Stu I restriction site at the 5′ end, and the reverse primer had an Hin dIII site at the 3′ end. .. PCR amplification with the primers added the restriction sites to the ends of the PCR products.

    Amplification:

    Article Title: Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
    Article Snippet: .. The amplified fragment was inserted into the pQE30 vector (Qiagen, Hilden, Germany), which was then transformed into Escherichia coli M15 cells. .. The ChACBP protein was induced, ultrasonicated, and loaded onto Ni–NTA Sefinose (Qiagen).

    Article Title: Monoclonal Antibodies against the Fusion Peptide of Hemagglutinin Protect Mice from Lethal Influenza A Virus H5N1 Infection ▿
    Article Snippet: .. The HA gene and the HA2 gene were amplified from the cDNA and cloned into pQE-30 vector (Qiagen, Germany), using standard cloning techniques for expression in bacteria. .. The clones were transformed into Escherichia coli M15/pREP4 competent cells for protein expression.

    Article Title: Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
    Article Snippet: .. The amplified fragment was inserted into the pQE30 vector (Qiagen, Hilden, Germany), which was then transformed into Escherichia coli M15 cells. .. The ChACBP protein was induced, ultrasonicated, and loaded onto Ni–NTA Sefinose (Qiagen).

    TA Cloning:

    Article Title: A thermostable endonuclease III homolog from the archaeon Pyrobaculum aerophilum
    Article Snippet: .. The PCR product was cloned into pCR2.1-TOPO vector using TOPO TA Cloning kit (Invitrogen, Carlsbad, CA) and subsequently subcloned into the bacterial expression vector pQE30 (Qiagen, Chatsworth, CA) between the Bam HI and Hin dIII sites. ..

    Purification:

    Article Title: Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
    Article Snippet: .. PCR product was then digested by Sac I and Hin dIII, purified and ligated with the pQE-30 vector, which was digested with the same endonucleases. ..

    Expressing:

    Article Title: A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus
    Article Snippet: .. Construction of expression plasmids For the expression of HCV proteins in E. coli , corresponding coding sequences were cloned into the histidine fusion expression vectors, pET32a(+) and pQE30, or the GST-tag expression vector, pGEX-4T-2, as shown in and . .. Recombinant plasmids were examined and confirmed by PCR amplification, restriction enzyme digestion and DNA sequencing.

    Article Title: Monoclonal Antibodies against the Fusion Peptide of Hemagglutinin Protect Mice from Lethal Influenza A Virus H5N1 Infection ▿
    Article Snippet: .. The HA gene and the HA2 gene were amplified from the cDNA and cloned into pQE-30 vector (Qiagen, Germany), using standard cloning techniques for expression in bacteria. .. The clones were transformed into Escherichia coli M15/pREP4 competent cells for protein expression.

    Article Title: A thermostable endonuclease III homolog from the archaeon Pyrobaculum aerophilum
    Article Snippet: .. The PCR product was cloned into pCR2.1-TOPO vector using TOPO TA Cloning kit (Invitrogen, Carlsbad, CA) and subsequently subcloned into the bacterial expression vector pQE30 (Qiagen, Chatsworth, CA) between the Bam HI and Hin dIII sites. ..

    Article Title: Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis
    Article Snippet: .. Expression vector pQE30 (Qiagen) contains the T5 promoter for high-level expression, a ribosome binding site, and × histidine coding sequences followed by a multiple cloning site. .. The vector also contains two lac operator sequences.

    Article Title: Unique Epitope of Bovine Immunodeficiency Virus Gag Protein Spans the Cleavage Site between p16MA and p2L †
    Article Snippet: .. For cloning of the deduced epitope into the pQE30Xa expression vector (QIAGEN), the forward primer had an Stu I restriction site at the 5′ end, and the reverse primer had an Hin dIII site at the 3′ end. .. PCR amplification with the primers added the restriction sites to the ends of the PCR products.

    Polymerase Chain Reaction:

    Article Title: A thermostable endonuclease III homolog from the archaeon Pyrobaculum aerophilum
    Article Snippet: .. The PCR product was cloned into pCR2.1-TOPO vector using TOPO TA Cloning kit (Invitrogen, Carlsbad, CA) and subsequently subcloned into the bacterial expression vector pQE30 (Qiagen, Chatsworth, CA) between the Bam HI and Hin dIII sites. ..

    Article Title: Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
    Article Snippet: .. PCR product was then digested by Sac I and Hin dIII, purified and ligated with the pQE-30 vector, which was digested with the same endonucleases. ..

    Transformation Assay:

    Article Title: Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
    Article Snippet: .. The amplified fragment was inserted into the pQE30 vector (Qiagen, Hilden, Germany), which was then transformed into Escherichia coli M15 cells. .. The ChACBP protein was induced, ultrasonicated, and loaded onto Ni–NTA Sefinose (Qiagen).

    Article Title: Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
    Article Snippet: .. The amplified fragment was inserted into the pQE30 vector (Qiagen, Hilden, Germany), which was then transformed into Escherichia coli M15 cells. .. The ChACBP protein was induced, ultrasonicated, and loaded onto Ni–NTA Sefinose (Qiagen).

    Binding Assay:

    Article Title: Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis
    Article Snippet: .. Expression vector pQE30 (Qiagen) contains the T5 promoter for high-level expression, a ribosome binding site, and × histidine coding sequences followed by a multiple cloning site. .. The vector also contains two lac operator sequences.

    Plasmid Preparation:

    Article Title: Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
    Article Snippet: .. The amplified fragment was inserted into the pQE30 vector (Qiagen, Hilden, Germany), which was then transformed into Escherichia coli M15 cells. .. The ChACBP protein was induced, ultrasonicated, and loaded onto Ni–NTA Sefinose (Qiagen).

    Article Title: A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus
    Article Snippet: .. Construction of expression plasmids For the expression of HCV proteins in E. coli , corresponding coding sequences were cloned into the histidine fusion expression vectors, pET32a(+) and pQE30, or the GST-tag expression vector, pGEX-4T-2, as shown in and . .. Recombinant plasmids were examined and confirmed by PCR amplification, restriction enzyme digestion and DNA sequencing.

    Article Title: Monoclonal Antibodies against the Fusion Peptide of Hemagglutinin Protect Mice from Lethal Influenza A Virus H5N1 Infection ▿
    Article Snippet: .. The HA gene and the HA2 gene were amplified from the cDNA and cloned into pQE-30 vector (Qiagen, Germany), using standard cloning techniques for expression in bacteria. .. The clones were transformed into Escherichia coli M15/pREP4 competent cells for protein expression.

    Article Title: Overexpression of Acyl-CoA-Binding Protein 1 (ChACBP1) From Saline-Alkali-Tolerant Chlorella sp. Enhances Stress Tolerance in Arabidopsis
    Article Snippet: .. The amplified fragment was inserted into the pQE30 vector (Qiagen, Hilden, Germany), which was then transformed into Escherichia coli M15 cells. .. The ChACBP protein was induced, ultrasonicated, and loaded onto Ni–NTA Sefinose (Qiagen).

    Article Title: A thermostable endonuclease III homolog from the archaeon Pyrobaculum aerophilum
    Article Snippet: .. The PCR product was cloned into pCR2.1-TOPO vector using TOPO TA Cloning kit (Invitrogen, Carlsbad, CA) and subsequently subcloned into the bacterial expression vector pQE30 (Qiagen, Chatsworth, CA) between the Bam HI and Hin dIII sites. ..

    Article Title: Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis
    Article Snippet: .. Expression vector pQE30 (Qiagen) contains the T5 promoter for high-level expression, a ribosome binding site, and × histidine coding sequences followed by a multiple cloning site. .. The vector also contains two lac operator sequences.

    Article Title: Lactobionic acid production by glucose–fructose oxidoreductase from Zymomonas mobilis expressed in Escherichia coli
    Article Snippet: .. PCR product was then digested by Sac I and Hin dIII, purified and ligated with the pQE-30 vector, which was digested with the same endonucleases. ..

    Article Title: Unique Epitope of Bovine Immunodeficiency Virus Gag Protein Spans the Cleavage Site between p16MA and p2L †
    Article Snippet: .. For cloning of the deduced epitope into the pQE30Xa expression vector (QIAGEN), the forward primer had an Stu I restriction site at the 5′ end, and the reverse primer had an Hin dIII site at the 3′ end. .. PCR amplification with the primers added the restriction sites to the ends of the PCR products.

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  • 99
    Qiagen expression vector pqe30
    Electrophoretic analysis of E. coli -expressed LF. (a) Proteins separated by SDS-10% PAGE and stained with Coomassie blue; (b) Western blot of the E. coli proteins containing LF, developed with a rabbit polyclonal LF antibody. Lanes: A, E. coli SG13009 cells without the vector; B, cells containing the vector <t>pQE30</t> without the LF gene; C, cells containing the construct pPG-LF1 (uninduced); D, cells expressing LF; E, periplasmic proteins of cells expressing LF; F, cytosolic proteins of cells expressing LF; G, inclusion bodies of cells expressing LF; H, LF purified from B. anthracis .
    Expression Vector Pqe30, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/expression vector pqe30/product/Qiagen
    Average 99 stars, based on 316 article reviews
    Price from $9.99 to $1999.99
    expression vector pqe30 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Electrophoretic analysis of E. coli -expressed LF. (a) Proteins separated by SDS-10% PAGE and stained with Coomassie blue; (b) Western blot of the E. coli proteins containing LF, developed with a rabbit polyclonal LF antibody. Lanes: A, E. coli SG13009 cells without the vector; B, cells containing the vector pQE30 without the LF gene; C, cells containing the construct pPG-LF1 (uninduced); D, cells expressing LF; E, periplasmic proteins of cells expressing LF; F, cytosolic proteins of cells expressing LF; G, inclusion bodies of cells expressing LF; H, LF purified from B. anthracis .

    Journal: Infection and Immunity

    Article Title: Expression and Purification of the Recombinant Lethal Factor of Bacillus anthracis

    doi:

    Figure Lengend Snippet: Electrophoretic analysis of E. coli -expressed LF. (a) Proteins separated by SDS-10% PAGE and stained with Coomassie blue; (b) Western blot of the E. coli proteins containing LF, developed with a rabbit polyclonal LF antibody. Lanes: A, E. coli SG13009 cells without the vector; B, cells containing the vector pQE30 without the LF gene; C, cells containing the construct pPG-LF1 (uninduced); D, cells expressing LF; E, periplasmic proteins of cells expressing LF; F, cytosolic proteins of cells expressing LF; G, inclusion bodies of cells expressing LF; H, LF purified from B. anthracis .

    Article Snippet: Expression vector pQE30 (Qiagen) contains the T5 promoter for high-level expression, a ribosome binding site, and × histidine coding sequences followed by a multiple cloning site.

    Techniques: Polyacrylamide Gel Electrophoresis, Staining, Western Blot, Plasmid Preparation, Construct, Expressing, Purification

    Expression of r-EDIII-T protein in E. coli . (A) Map of the expression plasmid. The synthetic rEDIII-T gene was inserted into the BamHI and HindIII sites of plasmid pQE30, in-frame with the vector-provided ATG and six-His tag-encoding sequence. Abbreviations

    Journal:

    Article Title: Single Antigen Detects both Immunoglobulin M (IgM) and IgG Antibodies Elicited by All Four Dengue Virus Serotypes ▿Single Antigen Detects both Immunoglobulin M (IgM) and IgG Antibodies Elicited by All Four Dengue Virus Serotypes ▿ †

    doi: 10.1128/CVI.00145-07

    Figure Lengend Snippet: Expression of r-EDIII-T protein in E. coli . (A) Map of the expression plasmid. The synthetic rEDIII-T gene was inserted into the BamHI and HindIII sites of plasmid pQE30, in-frame with the vector-provided ATG and six-His tag-encoding sequence. Abbreviations

    Article Snippet: E. coli expression strain SG13009 (pREP4 [Kanr ]), the expression plasmid pQE30 (Ampr ), Ni-nitrilotriacetic acid (NTA) Super-flow resin, and anti-His (penta-His) monoclonal antibody were from QIAGEN (Hilden, Germany).

    Techniques: Expressing, Plasmid Preparation, Sequencing

    HCV genome and recombinant proteins. (A) The HCV genome contains a single major open reading frame (ORF) flanked by untranslated regions (UTRs). The 10 proteins encoded within the main ORF are indicated by alternated shading. (B) Genomic fragments of HCV were cloned into the prokaryotic expression vectors, pET32a(+), pQE30, or pGEX-4T-2, in-frame downstream of the 6-His-tag or glutathione S-transferase (GST)-tag coding sequence.

    Journal: International Journal of Molecular Medicine

    Article Title: A novel double antibody sandwich-lateral flow immunoassay for the rapid and simple detection of hepatitis C virus

    doi: 10.3892/ijmm.2012.1121

    Figure Lengend Snippet: HCV genome and recombinant proteins. (A) The HCV genome contains a single major open reading frame (ORF) flanked by untranslated regions (UTRs). The 10 proteins encoded within the main ORF are indicated by alternated shading. (B) Genomic fragments of HCV were cloned into the prokaryotic expression vectors, pET32a(+), pQE30, or pGEX-4T-2, in-frame downstream of the 6-His-tag or glutathione S-transferase (GST)-tag coding sequence.

    Article Snippet: Construction of expression plasmids For the expression of HCV proteins in E. coli , corresponding coding sequences were cloned into the histidine fusion expression vectors, pET32a(+) and pQE30, or the GST-tag expression vector, pGEX-4T-2, as shown in and .

    Techniques: Recombinant, Clone Assay, Expressing, Sequencing

    Expression and purification of PaNth protein. The 10% SDS–polyacrylamide gel contains the following samples: lysate of CSH100/pQE30 cells (lane 1), lysate of CSH100/pQE30 PaNth after IPTG induction (lane 2), PaNth protein eluted from Ni 2+ -NTA column (lane 3), 70°C heat-treated PaNth (lane 4) and 90°C heat-treated PaNth (lane 5). The gel was visualized by Coomassie Blue staining. PaNth protein is indicated on the right. Lane M contains molecular mass standards (Bio-Rad) as indicated on the left.

    Journal: Nucleic Acids Research

    Article Title: A thermostable endonuclease III homolog from the archaeon Pyrobaculum aerophilum

    doi:

    Figure Lengend Snippet: Expression and purification of PaNth protein. The 10% SDS–polyacrylamide gel contains the following samples: lysate of CSH100/pQE30 cells (lane 1), lysate of CSH100/pQE30 PaNth after IPTG induction (lane 2), PaNth protein eluted from Ni 2+ -NTA column (lane 3), 70°C heat-treated PaNth (lane 4) and 90°C heat-treated PaNth (lane 5). The gel was visualized by Coomassie Blue staining. PaNth protein is indicated on the right. Lane M contains molecular mass standards (Bio-Rad) as indicated on the left.

    Article Snippet: The PCR product was cloned into pCR2.1-TOPO vector using TOPO TA Cloning kit (Invitrogen, Carlsbad, CA) and subsequently subcloned into the bacterial expression vector pQE30 (Qiagen, Chatsworth, CA) between the Bam HI and Hin dIII sites.

    Techniques: Expressing, Purification, Staining