Journal: Frontiers in Microbiology

Article Title: Pushing the upper temperature limit of methanotrophy in continental hydrothermal ecosystems, active biological methane oxidation in hot springs of Yellowstone National Park

doi: 10.3389/fmicb.2026.1736896

Figure Lengend Snippet: Distribution and abundance of aerobic bacterial methanotroph phylotypes as a function of pH, temperature, chemical, and energy supply data. The size of the circle scales with the summed relative abundance of the methanotroph phylotypes. Gray circles are sites where relative abundance is <1%. Circles with an “x” are sites where bacterial aerobic methanotroph phylotypes were not detected. (A) The distribution across pH and temperature. (B) The distribution across the concentrations of dissolved O 2 and CH 4 in hot spring fluids. Included are the K m CH4 of Methylococcus capsulatus Bath and the K m O2 of Methylocystis . The line for the stoichiometric ratio of CH 4 to O 2 is also shown. (C) The distribution across energy supply for aerobic methane oxidation and the log ratio of the concentration of the electron donor (CH 4 ) and electron acceptor (O 2 ). The concentrations are corrected by the stoichiometry of the redox reaction and the line represents when they are stoichiometrically equal (ratio = 1).

Article Snippet: , Methylocystis sp. strain ATCC 49242 + 5,000 ppm CH4 ( ) , , 1950* , .

Techniques: Concentration Assay

Journal: Frontiers in Microbiology

Article Title: Pushing the upper temperature limit of methanotrophy in continental hydrothermal ecosystems, active biological methane oxidation in hot springs of Yellowstone National Park

doi: 10.3389/fmicb.2026.1736896

Figure Lengend Snippet: Reactant concentrations for anaerobic methane oxidation. (A) Concentrations of NO 2 − and CH 4 in hot spring fluids. Included are the K m NO 2 − and K m CH4 of “Candidatus Methylomirabilis oxyfera ” reported by . (B) Concentrations of NO 3 − and CH 4 in hot spring fluids. Included is the K m CH4 for an anaerobic methane oxidizing consortium using NO 3 − as an electron acceptor reported by . (C) Concentrations of SO 4 −2 and CH 4 in hot spring fluids. Included is the K m CH4 for an anaerobic methane oxidizing consortium using SO 4 −2 as an electron acceptor reported by .

Article Snippet: , Methylocystis sp. strain ATCC 49242 + 5,000 ppm CH4 ( ) , , 1950* , .

Techniques:

Journal: Frontiers in Microbiology

Article Title: Pushing the upper temperature limit of methanotrophy in continental hydrothermal ecosystems, active biological methane oxidation in hot springs of Yellowstone National Park

doi: 10.3389/fmicb.2026.1736896

Figure Lengend Snippet: Multivariate view of rates. The size of the circle scales with the square-root of the measured MOR. Rates for both the ambient gas (blue circles) and air-amended microcosms (red circles) are shown. (A) The measured rates of methane oxidation plotted as a function of the log ratio of the reactants and energy supply for aerobic methane oxidation. (B) The measured rates are plotted as a function of the log ratio of the concentrations of CH 4 and NH 3 and energy supply for aerobic methane oxidation. Sites where rates were measured and methane oxidizing taxa were detected are labeled with the detected taxa. Archaeal ammonia oxidizers are AOA and bacterial methanotrophs are MOB. Red MOB are sites where Verrucomicrobia methanotrophs were detected. Blue MOB are sites where Alphaproteobacteria were detected. Also shown is the [CH 4 ]:[NH 3 ] ratio which corresponds to the observed k cat / K m of the bacterial ammonia oxidizer Nitrosomonas europaea for either NH 3 oxidation ([CH 4 ]/[NH 3 ] < 0.004) or CH 4 oxidation ([CH4]/[NH 3 ] > 0.004) . The site labeled “Outlier” is a site with a relatively high rate of CH 4 oxidation despite have a [CH 4 ] to [NH 3 ] ratio <0.004.

Article Snippet: , Methylocystis sp. strain ATCC 49242 + 5,000 ppm CH4 ( ) , , 1950* , .

Techniques: Labeling