Journal: Science Advances

Article Title: Beta cell–targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices

doi: 10.1126/sciadv.aec9029

Figure Lengend Snippet: ( A ) Time series line plot representing the mean speed per well of T cell clone 4b (PPI 6–14 specific) in the presence of EndoC-βH2 red cells, 10 nM PPI ImmTAAI, 10 nM Tel ImmTAAI, or without molecule. ( B ) Boxplot representing the mean speed per well of all the time points shown in (A) (ns, not significant; **** P < 0.0001; one-way ANOVA with multiple comparisons analyzed by Tukey’s test). Data are representative of three independent experiments. ( C ) Growth curves of EndoC-βH2 red cells in the presence of T cell clone 4b at 1:1 E to T ratio, 10 nM PPI ImmTAAI, 10 nM Tel ImmTAAI, or no molecule over time. Time series line plot of live EndoC-βH2 red cell number over time normalized to the initial cell count at time = 0. ( D ) Bar plots representing the mean per well ± SD of live EndoC-βH2 red cells count at time = 30 hours normalized to the initial cell count at time = 0. Statistical differences were determined by one way ANOVA with multiple comparisons analyzed by Tukey’s test. ns, not significant. ** P < 0.01, *** P < 0.001. Data are representative of three independent experiments.

Article Snippet: The ImmTAAI molecules and PPI 6–14 TCRs can be provided by Immunocore for noncommercial, research purposes pending scientific review and a completed materials transfer agreement (MTA).

Techniques: Cell Characterization

Journal: Science Advances

Article Title: Beta cell–targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices

doi: 10.1126/sciadv.aec9029

Figure Lengend Snippet: ( A ) Experimental scheme depicts the coculture assay system used to evaluate the immunogenicity of sBC treated with ImmTAAI in the presence of HLA-A2 CAR, MelanA 27–35 –reactive (MART-1), IGRP 265–273 –reactive (clone 32), or PPI 6–14 –reactive (clone 15b) CD8 + avatars. Created in BioRender. Phelps, E. (2026) https://BioRender.com/n60mjw7 . ( B ) Specific lysis of sBCs by IGRP avatars after 24 hours of coculture or ( C ) PPI avatars after 48 hours of coculture with or without ImmTAAI treatment at each effector:target (E:T) ratio. n = 4. ( D ) Specific lysis for the 10:1 E:T ratio compared across all groups. **** P < 0.0001 by two-way ANOVA with multiple comparisons. n = 4. ( E and F ) Line plots show cell culture supernatant concentrations of proinflammatory T cell effector molecules and cytokines (granzyme A, granzyme B, granulysin, perforin, TNF, and FasL) produced by T cell avatars. P values are reported for paired samples using two-way ANOVA with Bonferroni’s correction for multiple comparisons.

Article Snippet: The ImmTAAI molecules and PPI 6–14 TCRs can be provided by Immunocore for noncommercial, research purposes pending scientific review and a completed materials transfer agreement (MTA).

Techniques: Co-culture Assay, Immunopeptidomics, Lysis, Cell Culture, Produced