pp1  (MedChemExpress)


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    Structured Review

    MedChemExpress pp1
    Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or <t>PP1</t> (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels ( n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K ( n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG ( n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)
    Pp1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp1/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
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    pp1 - by Bioz Stars, 2022-07
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    1) Product Images from "Fyn kinase regulates dopaminergic neuronal apoptosis in animal and cell models of high glucose (HG) treatment"

    Article Title: Fyn kinase regulates dopaminergic neuronal apoptosis in animal and cell models of high glucose (HG) treatment

    Journal: BMC Molecular and Cell Biology

    doi: 10.1186/s12860-021-00398-y

    Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or PP1 (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels ( n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K ( n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG ( n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)
    Figure Legend Snippet: Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or PP1 (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels ( n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K ( n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG ( n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)

    Techniques Used: Inhibition, Flow Cytometry

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    MedChemExpress pp1
    Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or <t>PP1</t> (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels ( n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K ( n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG ( n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)
    Pp1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pp1/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pp1 - by Bioz Stars, 2022-07
    93/100 stars
      Buy from Supplier

    93
    MedChemExpress 1nm pp1
    Genetical inhibition of ErbB4 kinase activity eliminates the effects of KD on inhibitory synaptic activity and seizure resistance. A Intraperitoneal injection of <t>1NM-PP1</t> (0.1 g/g body weight) inhibited ErbB4 phosphorylation in T796G mice in 30 min and recovered in 60 min. B Quantitative analysis of data in A. One-way ANOVA, for ErbB4, F (2,6) =0.4903, P =0.635; for P-ErbB4/ErbB4, F (2,6) =70.20, P
    1nm Pp1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1nm pp1/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
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    1nm pp1 - by Bioz Stars, 2022-07
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    96
    MedChemExpress phosphatase inhibitor cocktail 1
    Genetical inhibition of ErbB4 kinase activity eliminates the effects of KD on inhibitory synaptic activity and seizure resistance. A Intraperitoneal injection of <t>1NM-PP1</t> (0.1 g/g body weight) inhibited ErbB4 phosphorylation in T796G mice in 30 min and recovered in 60 min. B Quantitative analysis of data in A. One-way ANOVA, for ErbB4, F (2,6) =0.4903, P =0.635; for P-ErbB4/ErbB4, F (2,6) =70.20, P
    Phosphatase Inhibitor Cocktail 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or PP1 (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels ( n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K ( n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG ( n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)

    Journal: BMC Molecular and Cell Biology

    Article Title: Fyn kinase regulates dopaminergic neuronal apoptosis in animal and cell models of high glucose (HG) treatment

    doi: 10.1186/s12860-021-00398-y

    Figure Lengend Snippet: Inhibition of Fyn alleviates HG-induced cell injury by suppressing the mTOR/S6K signaling pathway. (A) Elevated cleaved caspase 3 levels induced by HG were rescued by rapamycin (100 nM) or PP1 (10 μM) treatment. PP1 inhibited p-Y416, but rapamycin did not change p-Y416 levels ( n = 5). β-actin, cleaved caspase 3 and Fyn were cropped from different parts of the same gel, and p-Y416 was cropped from a different gel. (B) PP1 or rapamycin inhibited mTOR/S6K ( n = 5). β-actin and S6K were cropped from different parts of the same gel, and p-S6K, mTOR and p-mTOR were cropped from different gels. (C) Flow cytometry showed that rapamycin or PP1 decreased the elevated cell apoptotic death induced by HG ( n = 3). (D) Decreased cell viability in response to HG was rescued by rapamycin or PP1 administration (n = 5)

    Article Snippet: Anti-tyrosine hydroxylase (TH) (ab112, Abcam, Cambridge, UK); Anti-TH (sc-25,269, Santa Cruz, Santa Cruz, USA); anti-cleaved Caspase 3 (9664; CST, Danvers, USA); Anti-Fyn (ab184276, Abcam, Cambridge, UK); anti-p-Y416 (6943; CST, Danvers, USA); anti-mTOR (2972; CST, Danvers, USA), anti-p-mTOR (Ser2448) (5536; CST, Danvers, USA); anti-p70 S6 Kinase (9202; CST, Danvers, USA); anti-p-S6K(Thr389) (97,596; CST, Danvers, USA); anti-β-actin (20536–1-AP, Proteintech, Wuhan, China), FITC-conjugated goat anti-rabbit IgG (Proteintech, Wuhan, China); FITC-conjugated donkey anti-mouse (Proteintech, Wuhan, China); Alexa Fluor 555-conjugated donkey anti-rabbit (Beyotime, Haimen, China); PP1 (HY-13084,MCE,New Jersey, USA); rapamycin (HY-10219, MCE, New Jersey, USA); all-trans retinoic acid (RA) (R2625, Sigma–Aldrich, Missouri, USA); glucose (15,023,021,Thermofisher, Waltham, USA).

    Techniques: Inhibition, Flow Cytometry

    Genetical inhibition of ErbB4 kinase activity eliminates the effects of KD on inhibitory synaptic activity and seizure resistance. A Intraperitoneal injection of 1NM-PP1 (0.1 g/g body weight) inhibited ErbB4 phosphorylation in T796G mice in 30 min and recovered in 60 min. B Quantitative analysis of data in A. One-way ANOVA, for ErbB4, F (2,6) =0.4903, P =0.635; for P-ErbB4/ErbB4, F (2,6) =70.20, P

    Journal: Cell & Bioscience

    Article Title: The ketogenic diet increases Neuregulin 1 expression via elevating histone acetylation and its anti-seizure effect requires ErbB4 kinase activity

    doi: 10.1186/s13578-021-00611-7

    Figure Lengend Snippet: Genetical inhibition of ErbB4 kinase activity eliminates the effects of KD on inhibitory synaptic activity and seizure resistance. A Intraperitoneal injection of 1NM-PP1 (0.1 g/g body weight) inhibited ErbB4 phosphorylation in T796G mice in 30 min and recovered in 60 min. B Quantitative analysis of data in A. One-way ANOVA, for ErbB4, F (2,6) =0.4903, P =0.635; for P-ErbB4/ErbB4, F (2,6) =70.20, P

    Article Snippet: 1NM-PP1 (HY-13942) and ITSA-1 (HY-100508) were from MedChemExpress.

    Techniques: Inhibition, Activity Assay, Injection, Mouse Assay